Page 1 Articles of 44 in PresS. Am J Physiol Endocrinol Metab (April 24, 2007). doi:10.1152/ajpendo.00627.2006
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Glucocorticosteroid-induced osteoporosis in adult primiparous Göttingen miniature pigs:
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effects on bone mineral and mineral metabolism
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K.E. Scholz-Ahrens 1, G. Delling 2, B. Stampa,3, A. Helfenstein 4, H.-J. Hahne 4 , Y. Açil 5, W.
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Timm 3, R. Barkmann 3, J. Hassenpflug 4, J. Schrezenmeir 1, and C.-C. Glüer3
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and Food, Kiel, Germany
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2
Department of Osteopathology, University Medical Center Hamburg-Eppendorf, Germany
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3
Medical Physics Research Group, Department of Diagnostic Radiology, University Hospital
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Institute of Physiology and Biochemistry of Nutrition, Federal Research Centre for Nutrition
Schleswig-Holstein, Campus Kiel
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4
Department of Orthopaedics, University Hospital Schleswig-Holstein, Campus Kiel
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5
Department of Oral and Maxillofacial Surgery, Kiel University, Germany
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Running title: Glucocorticosteroid induced osteoporosis in minipigs
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Correspondence: Dr. Katharina E. Scholz-Ahrens; Institute of Physiology and Biochemistry of
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Nutrition; Federal Research Centre for Nutrition and Food - Location Kiel; Hermann-
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Weigmann-Str. 1; D-24103 Kiel; Phone: +49 431 609 2474 /2460; Fax: +49 431 609 2472;
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E-mail:
[email protected];
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1 Copyright © 2007 by the American Physiological Society.
Page 2 of 44
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ABSTRACT
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Information on the pathophysiology of glucocorticoid-induced osteoporosis (GIO) is limited,
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since its clinical picture often reflects a combined effect of glucocorticoids (GC) and the
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treated systemic disease (i.e. inflammation and immobility). In 50 healthy adult (30 month old)
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primiparous Göttingen minipigs we studied the short-term (8 months, n=30) and long-term
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effect of GC (15 months, n=10) on bone and mineral metabolism longitudinally and cross-
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sectionally, as compared to a control group (n=10). All animals on GC treatment received
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prednisolone orally at a dose of 1.0 mg/kg body weight/d for 8 weeks, and thereafter at 0.5
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mg/kg body weight/d. In the short-term GC reduced bone mineral density (BMD) at the lumbar
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spine by -47.5±5.1 mg/cm3 from baseline (P