1 Table of Contents Supporting Information Figure 1 2 Supporting

Table of Contents Supporting Information Figure 1

2

Supporting Information Figure 2

3


4


5


6


8


9


10

1



Macroscopic aspect of lungs of BALB/c mice that have been saline-injected (left), or infected with 2.107 K. rhinoscleromatis (middle) or 2.104 Kp52.145 (right) five days post-infection.

2


A

resident monocytes

Granulocytes 07

2.0×10

Kr Kp Control

08

1.0×10

Kp

1.5×1007

7.5×1007

Control

1.0×1007

5.0×1007

5.0×1006

2.5×1007 0.0

Kr

Number of cells/lungs


1.2×1008

0

1

2

3

4

5

0.0

6

Days

0

Alveolar macrophages Number of cells/lungs


Kp Control

6×1005 4×1005 2×1005

2

3

4

5

Kp

3×1007

1×1007 0

6

Control

2×1007

C

0

1

2

Percent of total cells

3

4

5

6

Number of Mikulicz cells / mm2

Inflammatory Monocytes 2000

***

15

1500

10

1000

5

500

BALB/c

6

Days

B

0

5

Kr

Days

20

4

4×1007

Kr

1

3

Inﬂammatory monocytes

8×1005

0

2

Days

1×1006

0

1

C57BL/6

0

**

BALB/c

C57BL6

A) Kinetic of cells recruitment after infection with K. rhinoscleromatis and Kp52.145. Lung cells of BALB/c mice infected with 2.107 K. rhinoscleromatis, 2.104 Kp52.145 or salineinjected controls were isolated 1, 3 and 5 days post inoculation and stained for granulocytes (Gr1+ F4/80- CD11b+ CD11c-), resident monocytes (Gr1- F4/80+ CD11b+ CD11c-), alveolar macrophages (Gr1- F4/80+ CD11b- CD11c+) or inflammatory monocytes (Gr1+ F4/80+ CD11b+ CD11c-). Results show the number of each cell population in the total lung cells. Data are mean +/- sem and represent between 6 and 12 mice for each point from at least three independent experiments. B) Comparison of percentage of inflammatory monocytes between BALB/c and C57BL/6 mice infected with 2.107 K. rhinoscleromatis at three days post-infection. Data are mean +/sem. (*** , p