class titers to MSP-119 was significantly higher than total IgG (p=0.033;. 95%CI= -0.144 to ...... good malaria microscopy requires extensive training, vast technical knowledge ...... unit can review all safety parameters online. This overview ...... at baseline with a TBE - ELISA tests in secondary analyses in the ITT population.
150 domains of cg2 gene was analysed by nested PCR. Pfcrt-K76T mutation showed significant and complete association with in vitro CQ susceptibility status with 100% sensitivity and specificity. Pfmdr1-N86Y mutation also showed strong association with in vitro CQ resistance with 99.05% sensitivity and 100% specificity. Length polymorphism in cg2-kappa and omega repeat domains showed significant but incomplete association with CQ resistance with lower sensitivity (88.57% and 67.61% respectively) and specificity (65.22% and 60.87 respectively). Furthermore, strong linkage disequilibrium was observed between alleles of codon 76 of pfcrt and alleles of codon 86 of pfmdr1 gene. The results show that Pfcrt-K76T and pfmdr1-N86Y mutations are good diagnostic molecular markers of CQ resistance in NE India while length polymorphism in kappa and omega repeat domains of cg2 gene are not good markers. Thus, Pfcrt-K76T and pfmdr1-N86Y mutations either alone or in combination can be used as epidemiological tools for surveillance of CQ resistance in India, especially in areas like NE Indian states where falciparum malaria is highly endemic and CQ resistance is high.
522 RESPONSE OF FALCIPARUM MALARIAL PARASITE TO STANDARD TEST DOSE OF CHLOROQUINE IN PUNJAB, PAKISTAN Saleem M. Rana Directorate General Health Services Punjab, Lahore, Pakistan Present study represents the results of a 28-day follow-up of 404 patients treated with chloroquine for uncomplicated falciparum malaria in five districts of Punjab, Pakistan. Chloroquine resistance checked in the subjects harboring Plasmodium falciparum, using in vivo techniques was 35.4%, with 31.2% RI and 4.2% RII and nil RIII. During the non-transmission seasons for the years 1999-2005, among the rural populations Among five districts maximum P. falciparum (%) noted in Muzaffargarh (29.2) and minimum in Jhang (20.8). Among the five districts, maximum RI (35.2%) and RII (5.4%) were noted in Multan. Maximum resistance was noted in 5-15 year age group, but with a low RII (3%) value. Two patient characteristics were found to be independent and important predictors of resistance in a Cox proportional hazards model i.e. 5-15 years age group and parasitemia count >6000/μl. The weekly risk of resistance (%) was 18.7, 35.4, 19.0 and 25.0 during 1st, 2nd, 3rd and 4th week respectively, with lowest in 1st and highest in 2nd week. During the 28-day test 5 cases (1.2%) did not complete follow-up. From seven districts of the Punjab, 604 cases of P. falciparum satisfied the entry criteria for in vitro test. Out of 604 subjects with uncomplicated malaria 228 were treated with chloroquine, showed overall 38.6% resistance, with higher values in males (79.5%) than females (20.4%).192 subjects tested with basoquine in six districts of Punjab, showed 34.8% overall resistance with higher values in males (72%) than in females (28.35%). Another 192 subjects tested with sulphadoxine / pyrimethamine in six districts of Punjab, showed 5.7% resistance with higher values in males (63.6%) than females (36.4%) (p
