poison ivy extract is applied to the skin and bi- .... (Sepratech Corp., Oklahoma City, OK) as described .... in vivo model of inflammation, a poison ivy/oak mix-.
American Journal of Pathologv, Vol. 144, No. 2, February 1994 Copyrnght (0 American Society for Investigative Pathology
4A1 1, A Monoclonal Antibody Recognizing a Novel Antigen Expressed on Aberrant Vascular Endothelium Upregulation in an in Vivo Model of Contact Dermatitis
Alisa E. Koch,*I1¶ Brian J. Nickoloff,tt Jan Holgersson,t** Brian Seed,t** G. Kenneth Haines,tll James C. Burrows,*II and S. Joseph Leibovich§11 From the Departments of Medicine,* Pathology,t Molecular Biology,* and Basic Sciences,§ Nortbwestern University Medical and Dental Schools,11 and V. A. Lakeside Medical
Center,9 Chicago, Illinois; Massachusetts General Hospital, Boston, Massachusetts;** and The University of Michigan Medical Center,tt Ann Arbor, Michigan
We describe the production and characterization of a novel monoclonal antibody (MAb) that recognizes a human endothelial ceU antigen expressed mainly in inflamed and malignant disease states. We have used immunohistochemistry to determine the spectrum of reactivity of this MAb compared with that of a MAb to factor VIIIrelated antigen (MAb FVIII). MAb 4AII does not react with several myeloid or lymphoid ceU lines or with peripheral blood ceUs. Unlike MAb FVIII, MAb 4AII does not react with platelets. MAb 4AII reacts with most vascular endothelial ceUs in lymphoid tissue but withfew (
