Oct 2, 2014 - Ansarin - Khalil. Kadivar - Mohammad Rahim. Aslan Abadi - Saeed. Karimi - Abdollah. Badiee - Parisa. Kazemi - Abdolhasan. Bayat Makou - ...
8thInternational Congress of Clinical Microbiology September30 - October2, 2014
TABRIZ-IRAN
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In the Name of Allah, The Most Beneficent, The Most Merciful
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Special thanks go to: . Iranian society of pediatrician,EastAzarbaijan . Pediatric health research center . Prof.Alborzi clinical microbiology center . Research Center of Infection &Tropical Diseases . Pediatric Infections Research Center (PIRC) . Iranian Society of Medical Microbiology (ISMM)
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President: Abdolvahab.Alborzi, MD Professor of pediatric infectious Academic secretary: Shahram.Abdoli Oskuei, MD Professor of pediatric infectious Executive secretary: Babak.Abdi Nia, MD Professor of pediatric infectious Academic and Executive director: Mohammad.Khaneshi Medical student of Tabriz University of medical science 4
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Abdinia - Babak
Ghanaee - Roksana
Abdoli Oskuee - Shahram
Gharashi - Ziaeddin
Aghazadeh - Mohammad
Ghooya - Mohammad Mahdi
Ahangharzadeh Rezaee -
Hamedi - Abdolkarim
Mohammad
Hasani - Alka
Akbarzadeh - Amir Hosein
Hejazi - Mohammad Esmaeel
Alami - Mohammad Hasan
Hiradfar - Amir Ataollah
Alborzi - Abdolvahhab
Hosein Poorfeiz - Abbas Ali
Amirian - Mohammad Hadi
Irajian - Mohammad Mahdi
Ansarin - Khalil
Jamali Doost - Marziyeh
Aslan Abadi - Saeed
Kadivar - Mohammad Rahim
Badiee - Parisa
Karimi - Abdollah
Bayat Makou - Zhinoos
Kazemi - Abdolhasan
Chavoshzadeh - Zahra
Khaneshi - Mohammad
Ebrahimi - Elaheh
Mardani - Masood
Fahimzad - Seyyed Ali Reza
Moaddab - Reza
Fallah - Fatemeh
Moghaddaspoor - Seyyed
Farajnia - Safar
Esmaeel
Ghalee Gholab Behbahan -
Mokhtari - Talaat
Afshin
Moltajaee - Mohammad Sadeq
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Mostafavi - Seyyed Naser
Varshochi - Mojtaba
Naghili - Behrooz
Zahraee - Mohsen
Nahayi - Mohammad Reza Nazemiyyeh - Masood Oboodi - Barat Pooladfar - Gholam Reza Poorabbas - Bahman Rafiee Tabatabayi - Seddiqeh Rahbar - Mohammad Rahmani - Asaad Rashedi Rasooli - Parisa Rasoolinezhad - Mehrnaz Rezazadeh - Shahin Sanayi - Anahita Sayyahfar - Shirin Shiva - Siamak Shirvani - Fariba Shokri - Nazila Soltani - Jafar Soroosh - Hosein
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Abbasi - Mohammad
Farajpoor - Ramin
Abdinia - Babak
Habibi - Mohammad
Abdoli Oskuee - Shahram
Hesari - Elham
Abolhasani - Hesam
Hoseini - Saeed
Afsari - Foroohar
Irankhah - Shadi
Aghajamal - Nahal
Jadidi - Mahdiyeh
Aghazadeh - Mohammad
Javadzadeh - Mohammad
Ahangharzadeh Rezaee -
Reza
Mohammad
Javan - Akbar
Akbarzadeh - Amir Hosein
Karimzadeh - Arezoo
Azimpoor - Saeedeh
Khaneshi - Mohammad
Davarpanah - Tala
Khorsand - Mohammad
Ebrahimi - Elaheh
Mahdipoor - Shahab
Ebrahimi - Pooya
Moltajaee - Mohammad Sadeq
Esmaeeli - Saeed
Nazari - Roghayyeh
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Panjeh - Farshad Pasha Abgharmi - Ali Reza Rahimzadeh - Maryam Rahmani - Asaad Rasooli - Parisa Rezazadeh - Shahin Sadeghifard - Sepehr Salari - Saghar Shahbazi - Roghayyeh Shahi - Mohammad Shokri - Nazila Shokri - Parisa Soltani - Zahra Taheri - Tahereh Tasdighi - Fereshteh Yoosefi - Behzad
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Bacteriology Virology Parasitology Mycology Immunology Tropical infections Drug-resistance Nosocomial infections Infection control Vaccination Zoonotic infections
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PRESENTATION 11
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O-1 Diarrheagenic Escherichia coli (DEC), as a main cause but less considered in children diarrhea Mohammad Ahangarzadeh Rezaee, Gholamhasan Shahbazi, Alka Hasani, Mohammad Asgharzadeh, Ali Akbar Rahim-Rahimi, Masoud Alebouyeh Tabriz Infectious and Tropical Diseases Research Center, Department of Microbiology, Department of Laboratory Sciences, Tabriz University of Medical Sciences, Tabriz, Iran Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Introduction and objectives: Despite the obvious role of pathogenic E.coli in children diarrhea; however, due to traditional beliefs of most physicians about the main causes of diarrhea and also inability of usual laboratory methods in differentiation of DEC from the commensal kinds, the importance of these entropathogens are often overlooked. This study aims to investigate the role of pathogenic E.coli as diarrheal causes in children. Materials and methods: In a nine months period, 303 diarrheal samples from children with gastroenteritis were collected. All of samples were negative for parasitical agents, Salmonella and Shigella species. Stool specimens were processed according to standard microbiological methods. Confirmed isolates as E.coli were evaluated for harboring virulence genes using multiplex-PCR. Antibiotic resistance pattern of DEC isolates were determined using disk diffusion method according to CLSI standards. Results: 194 isolates were identified as E.coli using conventional phenotypic tests. Of these, 35.05% isolates were identified as DEC based on the results of PCR reactions. The highest and lowest rate of antibiotic resistance was observed against ampicillin (83.8%) and gentamicin along with ciprofloxacin (10.3%), respectively. 38.2% of isolates were resistant against ceftriaxone. Conclusion: Similar to previous studies, our results confirmed the significant contribution of DEC as one of the major causes of gastroenteritis in children. Therefore, given the ineffectiveness of conventional methods in identifying these strains, equip of pediatric referral centers with molecular tests are necessary. Moreover, physicians should be more considering about these entropathogens in their differential diagnosis, especially when the report of S/C is “NORMAL FLORA”.
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O-2 Cytoplamic membrane proteomics of fluconazole resistance gene in Aspergillus fumigatus Abdolhassan Kazemi Tropical & Infectious Disease Research Center, Tabriz Uni. of Med. Sci., Tabriz, Iran Introduction and objective: Clinical treatments of Aspergillus fumigatus as an opportunistic pathogen with fluconazole may fail due to the facade of resistance to this azole. This survey demonstrated that antifungals with a basic mechanism of action lead to comparable impact at the proteome level and that a proteomics approach can be used to discriminate different antifungals, with the assure to become a valuable utensil to study azole of unknown mechanism of effect to opportunistic fungi. Materials and methods: Protein dots were in-gel digested and resulting peptides were analyzed using MALDI-TOF MS and/or nano-ESI MS/MS. MS data were aligned against the NCBInr and the Genome Therapeutics Corporation PathogenomeTM databases using the MS-Fit and MS-Seq and/or MS-Edman programs respectively. Results: 567 amino acid obtained chain was matched with identity and unambiguously with an amino acid sequence present in the protein database. Analyzing of obtained protein using bioinformatics tools showed that fluconazole resistance gene encoded a structural protein for cell membrane with exact proteomic characters as shown at above diagram. It is notable that some of the induced proteins such as enolase, HSP90p and Fbalp have been shown to be present in the cell wall of A. fumigatus. More expression of these proteins may reproduce stress at the cell surface and is maybe directly linked to the effect of mulundocandin. Conclusion: Further studies with antifungal compounds acting at the same cellular level such as the nikkomycins, which inhibit chitin synthesis, would be helpful to confirm this hypothesis.
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O-3 Multi-drug resistant Acinetobacter baumannii: molecular analysis of emerging foe in Tabriz hospitals Alka Hasani, Akbar Hasani, Mahdieh Sadegifard Research Center of Infectious Diseases and Tropical Medicine and Department of Microbiology, Faculty of Medicine Sciences, Tabriz University of Medicine Sciences, Iran Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medicine Sciences, Iran Department of Microbiology, Islamic Azad University of Zanjan, Iran Introduction and objectives: Multidrug-resistant Acinetobacter baumannii, a rapidly emerging pathogen in the health care setting belong to one of three European clones I, II and III, which correspond to sequence type groups 2, 1 and 3 in sequence typing scheme. Last five years have witnessed this bacterium as multi drug or even pan resistant. To analyze the emergence of beta lactam, carbapenem, rifampin and aminoglycoside resistance among hospital strains of A. baumanii and type them by sequence-based typing. Materials and methods: Genotypically identified 95 A. baumannii were subjected to antibiotic susceptibility by disk agar diffusion according to CLSI. PCR was performed for detection of PER, IMI, VIM and ANT, AAC, APH resistance genes and class 1,2 and 3 integrons. Later, strains were typed by sequence typing and efficacy of pipercillin, meropenem, rifampin and ampicillin- clavulanic acid was analyzed by E- test. Results: On genotypic identification, 77 strains harbored both blaOXA-51-like and blaOXA23like genes. The aminoglycoside resistance ranged from 3.2- 43%. Class 1 integron was found in 47 strains. Interestingly, 23 strains carried integron of class 2. Around 72.13% and 49.18% of them showed resistance toward imipenem and pipercilin/tazobactam respectively. Resistance of A.baumanii strains towards were pipercillin, meropenem, rifampin and ampicillin- clavulanic acid ranged from 60- 84% on MIC test. By sequence typing strains belonged to type 1 or European clone II. Conclusion: Acinetobacter baumanii isolated in our geographical region belong to the clone persisting in most of the European and neighboring countries and are highly resistant towards most of the antibiotics.
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O-4 Ventilator associated pneumonia in neonates and children Aelami MH, Khademi GR, Alamdaran A, Lotfi M Aelami Mohammad Hassan MD, Department of pediatrics & Infection Control & Hand Hygiene Research Center, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Introduction and objectives: Ventilator-associated pneumonia (VAP) is common in mechanically ventilated neonates and children and has high morbidity and mortality in these patients. There is little information about rates and risk factors of VAP in Iran. Materials and methods: In a prospective study from 2012 to 2013 all neonates and children that mechanically ventilated for ≥48 hours in two ICUs of Dr Sheikh hospital in Mashhad enrolled in this study. VAP was defined for neonates and children according to CDC criteria. Results: Among 130 ventilated patients for ≥48 hours, 29 developed VAP (VAP Rate=22.3%).Mean age was 1 month. Sixty percent of the patients were male. The mean duration of admission was longer in patients with VAP compared to patients without VAP (29.31 days vs.8.45, P value0-15 year(s). The most common Shigella isolates were S.flexneri 28(53.8%) followed by S.sonnei14 (26.9%), S.dysenteriae 6(11.5%) and S.boydii 4(7.7%). The most common drug resistance among all age-groups was to Trimethoprim-sulphamethoxazole. All strains of S. flexneri, S. boydii, and S. dysenteriae were fully susceptible to Ceftriaxone and Ciprofloxacin.
Conclusion: These findings suggest reconsideration of the experimental use of these antimicrobial agents for shigellosis. A further study is required to assess additional antimicrobial agents.
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P-4 Extended-spectrum beta-lactamases detection in clinical specimens Rashid Ramazanzadeh, Pegah Shakib, Samaneh Rouhi, Babak Shahbazi, Noushin Abdolmaleki, Maryam Karimi Cellular & Molecular Research Center and Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran Introduction and objectives: Extended-spectrum beta-lactamases (ESBL) are enzymes that are produced by gram negative bacteria such as Enterobacter cloacae, Citrobacter freundii, Serratia marcescens, and Pseudomonas aeruginosa. More than 340 beta-lactamase enzymes are detected. Bacteria that produce ESBL are resistant to different antibiotics. So, aim of this study was ESBL detection from isolated bacteria in clinical specimens, Kurdisatan Hospitals, Sanandaj, Iran. Materials and methods: 2000 blood samples were collected from Kurdistan province Hospitals, Sanandaj, Iran (January 2007 to January 2008) (October 2012 to November 2012). PCR method for ESBL detection was applied in this study by using from ampicillin and penicillin resistance gene (TEM) and sulfhydryl variable (SHV) gene primers. Result: Different bacteria were isolated of samples: E. coli, Enterobacter spp., Klebsiella spp., P. aeruginosa, Salmonella spp., C. freundii, S. maltophilia, also S. aureus, and S.epidermis. 11.46% and 12.5% of bacteria were positive for TEM and SHV enzymes, respectively. Conclusion: ESBL producer strains among clinical isolates are increasing, appropriate method for ESBLs detection and appropriate use of antibiotic are necessary.
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P-5 Determination of vancomycin minimum inhibitory concentration in Staphylococcus aureus by E-test Rashid Ramazanzadeh, Samaneh Rouhi, Babak Shahbazi, Mazaher Khodabandehloo, Abbas Manafi, Hanar Narenji, Pegah Shakib Cellular & Molecular Research Center and Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran Introduction and objectives: Staphylococcus aureus is an important pathogen. This organism has resistance to different antibiotics. Different disease caused by drug-resistant S. aureus in hospitalized patients and community. Vancomycin is a antibiotic for infections treatment that caused by S. aureus. A vancomycin treatment failure with increased vancomycin minimum inhibitory concentration (MIC) is a problem in S. aureus infection. Aim of this study was determination of vancomycin MIC in isolated S. aureus. Materials and methods: 88 isolates of S. aureus strains were isolated from clinical and environmental specimens that were collected during 2011 and 2012 years. These strains were recognized with standard biochemical test including; coagulase, DNase, manitol salt agar test. Vancomycin MIC determination was performed E-test method in isolated S. aureus in Sanandaj city, Kurdistan province, Iran. Results: of 88 isolated S.aureus strains, 34% had MIC 0.5 mg/L, 5.6% had MIC 1.5 mg/L and MIC 2mg/L. The range of MIC was 0.19 - 1.5 mg/L. In this study no vancomycin resistant S.aureus or vancomycin intermediate were identified and all of isolates were sensitive. Conclusions: Vancomycin resistance is a concern in S.aureus treatment. Vancomycin is as effective drug for staphylococcal infections and also, resistant isolates are increasing, so necessary accurate and continuous surveillance should be considered in health programs.
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P-6 The prevalence of nosocomial infection and their resistance against antibiotics Nayyereh Agakhani Sanagoo, Azat Gaemi Department of the treatment center, Golestan University of Medical Sciences Introduction and objectives: The main problem of hospital infections - care centers in the world that is different in different countries in terms of intensity and weaknesses. Therefore, studies examining the function of the quality of hospital infection control measures can be improved by understanding the weaknesses of the. Materials and methods: The paper summarizes 21 years of research from 1373 to 1389 the incidence of nosocomial infections in the site IranMedex.com set is provided. Results: The findings showed that most bacteria, Klebsiella, Pseudomonas, Staphylococcus aureus, Kelly Shea was Asher. And imipenem were the most active antibiotics. Was the most common bacterium Pseudomonas burn Drzkhm. Venous catheter was 98% of the Enterobacteriaceae. The resistance patterns of gram-negative bacilli in nosocomial infection with Klebsiella Ian were 40% common germs. Antibiotic resistance in bacterial causes of neonatal sepsis is the most common germs resistant coagulase-negative staphylococci, 93% of the CLS has never Asylyn. Golden Staph infection is the most common germs in the intensive care unit and all of them were resistant to methicillin. NICU and PICU nosocomial infections in the study of the most common bacterium Pseudomonas aeruginosa 3/24% of the hospitals use surgical suction action of the common risk factors of nosocomial infection. Conclusion: The incidence of infection in patients with antibiotic-resistant bacteria and their resistance in vitro more attention to disinfecting hospital environments, managing these patients and requires the availability of appropriate antibiotics.
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P-7 Hepatitis B vaccination and how to respond to Iran N. Aghakhani, Abdul Moradi Department of the treatment center, Golestan University of Medical Sciences Introduction and objectives: Chronic type B hepatitis is an important public health around the world from an asymptomatic infection to symptomatic chronic hepatitis - cirrhosis and hepatocellular cancer varies. More than two billion of the world population has to deal with the hepatitis B virus in HBV carriers Andvdr estimated 350-400 million people worldwide have chronic hepatitis B infection leads to the death of 500,000 to 1,200,000 years. Disease in the National Health Plan in 1378, which was 7/1% of the general population, seems HBSAg and 35% were positive for HBV deal with the Iranians, and 3-2% of the populations are chronic carriers. Because there is a good vaccine against the disease, vaccination is the best way to prevent this disease. Materials and methods: A review of studies conducted in searching through different sites with a total of 4,031 people about 15 articles that were included in the study of vaccination against HBV was studied. Results: A review of studies conducted in searching through different sites with a total of 4,031 people about 15 articles that were included in the study of vaccination against HBV was studied. Conclusion: A total of 15 papers on hepatitis B vaccination on the 4,031 people surveyed, about 54/15% partial or no response to vaccination antibody titers were low. About 9/65% complete vaccination and antibody response or protection was good. Disease in the community thought.
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P-8 Cedar epidemiology of hepatitis B (Review) N. Aghakhani, Abdul Moradi Department of the treatment center, Golestan University of Medical Sciences Introduction and objectives: Hepatitis is a general term inflammation of the liver. Among the major hepatitis, Hepatitis B (HBV) is asymptomatic hepatitis B appears through skin contact or relatives by blood, sexual fluids Vamyzsh body is transferred. Those who are successfully vaccinated against HBV and anti-HBs is high, they are safe. Seroepidemiological Survey of Hepatitis B in the area of health care can guide the treatment of cholera and disease due to routine immunization in the assessment done in the case show. Thus the evaluation of the above-mentioned took objectives. Materials and methods: The papers on this site iranmedex. ir about Hepatitis B is presented in this paper. In this study, we studied over 260 articles on hepatitis B in terms of the highest percentage of positive HBSAg populations are. Results: This study showed that the prevalence rate of 88.7%, the highest percentage in Tehran there. Studies could also investigate the minimum 35 per cent prevalence of 0.0% in Kashan there Bamyzan HBSAg positive rate among addicts in the province Bamyzan 2.7% to 46% and lowest in the province, there is a maximum rate of 88.7% among patients in Tehran and at least 8. 0% in Bushehr is located in a Table is shown. Conclusion: The rate of infection in the community is high. Vmkhsvsa Among high-risk patients such as addicted to certain diseases seen Vmbtlayan seem to control the disease in the community and people with the disease are screening positive HBSAg people are in contact, should be vaccinated.
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P-9 Development of a sensitive and rapid Nested RT-PCR method for detection of all three types of poliovirus as an adventitious agent in cell substrates used in vaccine production Ali Salimi Jeda, Ashraf Mohammadi, Behnam Alirezaie Department of Human Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran Introduction and objectives: Although cell cultures are suitable substrate for production of biological products such as vaccines, they might be contaminated with adventitious agents and add them to the final product unexpectedly. Major sources of contamination are all raw materials such as virus seeds and cell banks. So every sensitive cell culture might theoretically be unexpectedly contaminated with exogenous viruses in the site of production. The aim of this study was to describe a rapid and sensitive method for detection of poliovirus as an adventitious agent in primate cells and virus seeds. Material and methods: The most highly conserved regions in the genome of the three serotypes were identified and primers were designed for these region. So the RT-PCR and Nested RT-PCR method programs for simultaneous detection of all three serotype of polioviruses was developed. In order to determine the limit of detection of the RT-PCR and Nested RT-PCR methods 10-fold serial dilutions of all three types of poliovirus with known titers were prepared. All steps including RNA extraction, cDNA synthesis, RT- PCR and Nested RT-PCR were employed for each serial dilutes of all three types of poliovirus. Results: The use of nested RT-PCR can successfully detect all three serotypes of polioviruses. The detection limit was calculated as 316.3 CCID50/ml, and 0.003 CCID50/ml in RT-PCR and Nested RT-PCR, respectively. Conclusion: The results showed that the established Nested RT-PCR method is sensitive and relatively rapid, which can be used for simultaneous detection of polioviruses in primate cells and virus seeds.
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P-10 Evaluation the antibiotic stewardship in Ghotbeddin Shirazi burn center during 5 years Amir Emami, Neda Pirbonyeh, Mitra Zardosht, Nahal Hadi, Zahra Zardosht, Maryam Kamali, Maryam Naderi Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Department of microbiology, Shiraz burn research center, Shiraz University of Medical Science, Shiraz, Iran Introduction and objectives: At present, drug resistance in the world, has become one of the most significant problems. The main predominant factor in enhancement of antimicrobial resistance is inappropriate use of this important compound especially in clinical centers and willfully drug consumption by the general public. According to the importance of antimicrobial stewardship programs we evaluate the prescribing of antibacterial agents in Ghotbeddin Shirazi burn center affiliated with Shiraz University of medical Sciences. Materials and methods: This cross sectional and questionnaire dependent study was retrospectively done in association between antimicrobial use and the kind of infection. In this evaluation total of 1350 file in over 5 years was evaluated. The outcomes of extensive implementation of antimicrobial stewardship were evaluated from the standpoint of antimicrobial use density, treatment duration, duration of hospital stay, occurrence of antimicrobial-resistant bacteria and medical expenses. Results: During the study period, 575 patients were admitted to the Ghotbeddin Shirazi burn hospital that 225 (39.2%) females and 350 (60.8%) males were included. Their mean age was 28.18±0.03 years (1 to 92 years). Antibiotic prescription for patients were vancomycin 250(43.6%) , keflex209(36.4%), cefazolin 206(35.9%), imipenem 187(32.6%), erythromycin 119(20.7%), ciprofloxacin 92(16%) , ceftazidime 82(14.3%), colestine 47(8.2%), keflin 47(8.2%), ampisulbactam 45(7.8%), gentamycin 44(7.7%), amikacin 28(4.9%), ceftriaxone 15(2.6%), clindamycin 10(1.7%) , cephalexin 10(1.7%), chlramphenicel 6(1%), tetracyclin 4(0.7%), metronidozol 3(0.5%), mapirocin 2(0.3%), coamoxiclove 1(0.2%). Conclusion: Extensive implementation of antimicrobial stewardship led to a decrease in the inappropriate use of antibiotics, saving in medical expenses, reduction in the development of antimicrobial resistance and shortening of hospital stay.
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P-11 Diagnosis of toxoplasmic infection in aborted fetuses by polymerase chain reaction method (Arak, IRAN) Zahra Eslamirad, Mahdi Mosayebi, Reza HajiHossein Assistant Professor, Department of Parasitology, Arak University of Medical Sciences, Arak, Iran MSc in Parasitology, Department of Parasitology, Arak University of Medical Sciences, Arak, Iran Introduction and objectives: Toxoplasma gondii is a protozoan parasite that can infect humans and warm blooded animals. In spite of toxoplasmosis being generally clinically asymptomatic, a primary infection during pregnancy in women and animals may cause abortion or perinatal death. Recently molecular techniques such as PCR have emerged as reliable methods of Toxoplasma gondii diagnosis. This study describes the performance of molecular test for diagnosis of toxoplasmic infection in tissues of human aborted fetuses. Materials and methods: The samples were collected from delivery room of maternity hospital, Arak- Iran, during one year. 87 spontaneous aborted fetuses were collected in separate container of preservative solution. Written consent was obtained from their mothers and demographic questionnaire was completed. Then 3 ml of blood sample was collected from mothers’ aborted fetus separately. DNA extraction from tissues of fetuses was performed and stored in -20° C for further use. The PCR reaction was performed by a pair of primers and PCR products were analyzed by electrophoresis. Results: The results showed that most of mothers that have abortion were in the age group of 20-29. There was not significant different between locations of living, contact with cats and eating undercooked food with abortion. The results of PCR amplification showed that none of the 87 samples from aborted fetuses were infected with T. gondii. Conclusion: In this study was not found evidence of Toxoplasmosis trace in aborted fetuses, and it appears that Toxoplasma was not cause to spontaneous abortion in this area of Iran.
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P-12 Toxocara canis IgG in asthmatic children and juvenile Mahdi Mosayebi, Latif Moeinim, Reza Hajihossein, Mojtaba Didehdar, Zahra Eslamirad Parasitology and Mycology Dept., Medicine faculty, Arak medical Sciences University, Sardasht Area,Arak,Iran Internal Medicine Dept., Medicine faculty, Arak medical Sciences University, Sardasht Area,Arak,Iran
Introduction and objectives: Human toxocariasis is a helminthozoonosis infection due to the migration of Toxocara larvae in to human organs such as the lungs or liver. Toxocara may be a possible etiologic agent of asthma. The aim of this study was to evaluation of anti Toxocara canis IgG antibodies in patients with Asthma (5-15 years) refered to pulmonary clinic in Arak city by Enzyme linked immune sorbent assay (ELISA). Materials and methods: serum samples from 110 children with confirmed asthma and 70 blank persons (without asthma) collected during one year and Toxocara IgG antibodies were examined by Enzyme linked immune sorbent assay (ELISA) test. Results were analyzed by SPSS software version 16. Results: Seroprevalence antibodies against Toxocara in children with asthma, with ELISA test were obtained in 1.8% (2 male persons), Among the 70 children (without asthma) in the control group were not antibodies against Toxocara Canis and were not significant gender and ،differences between the frequency of antibodies against Toxocara with age location (P>0.05). There were not significant differences between the frequency of antibodies against Toxocara with contact with dog, eating unwashed fruits and vegetables and eating raw or undercooked liver sheep (P>0.05). Conclusion: The Present study showed anti toxocara antibody in 2 children with asthma and determined the seroprevalence of Toxocariasis in Asthmatic children and juvenile in Arak.
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P-13 Hard ticks (family: Ixodidae) laboratory rearing from egg to larva emerge Abbas Bahrami, Mahdi Soliman Fallah, Zahra Dadashi, Darush Jalilian Savodjbolagh Health Network, Alborz University of Medical Sciences
Introduction and objectives: One of the most important groups of arthropods is ticks. Ticks transmission some diseases of bacterial, viral, rickettsial and parasitic to humans and animals. To understand the biology and ecology of the vectors, Laboratory rearing and eggs production is very essential. Materials and methods: In this study 45 hard ticks were collected from host animal (sheep, goats and camels) in fields of Alborz province and placed into a sterile test tube. We collected 17 ticks of Rhipicephalus sanguineus, 12 ticks of Rh. Bursa and 16 ticks of Hyalomma dromedarry. They have been reared at laboratory in incubator 25 °C. Results: A total of 45 ticks, 15 ticks were blood fed and 30 ticks were not blood fed. Ticks of feed have been reared at laboratory in incubator 25 °. The preoviposition periods were 12 days for Rhipicephalus sanguineus and Rh. Bursa. For Hyalomma dromedarry preoviposition were 10 days. Larva emerged in eggs to Rhipicephalus sanguineus and Rh. Bursa after 16 days and larva emerged in eggs to Hyalomma dromedarry after 18 days. Conclusions: We can study of ticks in laboratory and collection data about biology, time of oviposition, time of larva emerge and blood feed of ticks to manage of control of this disease vector and the best use from acaricides in control programs.
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P-14 Assessment of immunogenecity of alginate microparticle containing lipopolysacharide Salmonella paratyphi B with Diphtheria toxoid in mice Derafshrazm Azita, Shapouri Reza NO23, Alley kaj3, st shahid babaie,sq tavoos,mohammadiyeh city,Qazvin Introduction and objectives: Human salmonellosis is one of the most common and economically important zoonotic diseases.Salmonella organisms may also be found in animal feedstuffs, causing subclinical gastro-intestinal carriage or infectious disease in animals, particularly poultry and pigs.Today, developing modern vaccines is one the important challenges in medicine. The objective of this study was to prepare alginate microparticle containing conjugate of Lipopolysaccharide Salmonella paratyphi B with Diphtheria Toxoid. Materials and methods: After cultivation of bacteria, LPS was extracted with modified hot phenol method, dialysis, concentration, and electrophoresis and detoxified were done. To improve immunogenicity, the detoxified LPS were coupled to rec Exo A with ADH and EDAC and this conjugate purified by CL-2B sepharose column of gel filtration. Then four groups of female BALB/c mice (each group was included 15 mice) was selected. Vaccination was performed by three doses with two week interval. Then serum samples was collected and antibodies response against LPS was measured by ELISA method for total IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3. Results: Two weeks after first dose there was no significance different between antibodies titers in groups that were immunized by LPS and DT-LPS. But after second and third doses, DT-LPS showed significance increasing in all types of antibodies titers concentration against LPS in versus D-LPS. The results of anti-LPS inductions for total IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3 were observed LPS-DT > LPS>DT Conclusion: These results indicated that LPS from Salmonella paratyphi B increases antiLPS antibodies in conjugate form with Diphtheria Toxoid, and can be appropriate effective conjugate.
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P-15 Study on the prevalence of Giardia infection in humans referred to laboratory of Tabriz, Iran, hygiene center since April-March, 2012-2014 Sedigeh Sarafraz, Mahdi Parsaie, Reza Ansari, Samad Motiei Tabriz University of Medical Sciences,Central Laboratory of Tabriz Introduction and objectives: Giardia is one of the most important causes of debilitating diarrhea as epidemic and endemic in all over the world especially among children and tourists. As this parasite disturbs the absorption of fat- soluble vitamins in severe infection, steatorrhea, Anemia, weight loss can be seen. Cyst of giardia is resistant to unapropitions environmental condition and also this parasite has high resistance to cold, chlor hn drinkable water has no effect on cysts. Materials and methods: This study was done between different age groups reffered to laboratory of Tabriz hygiene center. Due to the facilitier and equipments of this center direct examination of smears normal salin for detection of this parasite was done. Results: Eventually 86000 samples which were sent to this center from April 2012- March 2014 (27 months), 392 cases, (0.4%) of them wer infected to giardia and males and children (7-11) have larger portion of these cases. Conclusion: Due to the high prevalence of this parasite in summer and among children became of susceptibility, diagnosis and refeered to physicians can be effective on prevention of increased prevalence of this parasite.
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P-16 Antibiotic resistance pattern and evaluation of metallo-beta lactamase genes including blaIMP-1 and blaVIM-1 types in Pseudomonas aeruginosa isolated from patients in Zabol hospitals Abbas Ganjali, Zahra Rashki Ghalehnoo, Forogh Heydari Zabol University of Medical Science Introduction and objectives: Nosocomial infection is one of the most important medical problems in developed and under developing countries which cause the spread of infectious diseases in society. Pseudomonas aeruginosa is an important opportunistic pathogen causes clinical infection among patients. The target of this study, determination frequency of Metallo- β-lactamase (MBL) genes (blaIMP-1 and blaVIM-1) in P. aeruginosa strains isolated from hospitalized patients in Zabol of PCR method. Material and methods: In this study, 500 clinical swabs were obtained from patients attending teaching hospital in Zabol, Iran. A total 100 P. aeruginosa isolates were identified by conventional biochemical test. Antibacterial susceptibility test for Cefexim, Ceftazidim, Ciprofloxacin, Imipenem, Piperacillin and Tobramycin was performed using disk diffusion (kirby-Buer) method. All Imipenem resistant isolates were screened for MBLs production by IPM-EDTA disk. DNA was extracted by boiling method and then DNA was used to determine the presence of MBLs genes (bla IMP- 1 and blaVIM-1) by PCR method. Results: Resistance rate of the isolated strains were as follows: Cefexim 98%, Ceftazidim 15%, Ciprofloxacin 7%, Imipenem 11%, Piperacillin 7% and Tobramycin 4%. IPM-EDTA disk method showed that 5 out of 11 (45/4%) of Imipenem resistant P. aeruginosa isolates were MBLs producers. The PCR method showed that 77 out of 100 and 12 out of 100 P. aeruginosa isolates were positive for blaIMP-1 and blaVIM-1 respectively.
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P-17 Aspergillus species is a frequent finding in the sputum of Iranian CFpatients Hamid Badali, Soheila Khalilzadeh 1 Invasive Fungi Research Centre (IFRC)/Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran, 2Pediatric Respiratory Diseases Research Center, NRITLD, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Introduction and objectives: Mutations in CFTR result in a defective mucociliary clearance with production of viscous and sticky bronchial mucus that facilitates colonization of the airways by various microorganisms which contribute to the progressive deterioration of lung function. Therefore, considerable attention has been paid to prevention and control of microbial growth, which has resulted in improvement of patient management and in a marked increase in the life expectancy of the patient. The objective of this study was to investigate the frequency of aspergillus species from respiratory specimens of Iranian CF patient, with in vitro susceptibility testing Material and methods: 42 patients diagnosed to have CF, based on positive sweat chloride tests, a characteristic clinical manifestation, or on mutations in CFTR protein, enrolled in the Respiratory Research Centre. Results: The most frequently isolated filamentous fungi were A. terreus (37.5%), followed by A. fumigatus and A. oryzae (25%). A higher proportion of patients with persistent Aspergillus colonization had ABPA than those with no organisms. Finally, male were numerically more often persistently colonized with Aspergillus. A. terreus was the predominant species which colonized in the majority of CF cases that probably due to resistant to antifungal drug like AmB. Conclusion: However, in the present study the frequency of Aspergillus species are low, the possibility of fungal colonization and consideration of their clinical significance in these patients is highly recommended. Future studies and more factors which may be responsible for the increased isolation of fungi in CF need to be investigated.
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P-18 Knowledge, attitudes and practice towards HIV/AIDS among students of the University of Tabriz, Iran Payman Zare, Hemn Mohammadpour, Amin Azadian, Amir Mohammad Fadaei, Samad Hamidi Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Department of Clinical Psychology, Faculty of Education Sciences, University of Tabriz, Tabriz, Iran Introduction and objectives: Regular monitoring of knowledge, attitudes and practice for significant diseases like HIV/AIDS is helpful for policy makers to develop better control strategies and health education programs. Materials and methods: To evaluate the knowledge, attitudes and practice of students of University of Tabriz on HIV/AIDS, 420 students participated in a questionnaire-based, crosssectional survey during spring, 2011. Results: More than half of all participants had poor knowledge, attitudes and practice. None of the technician level students had a good knowledge level. However, 87.3% of participants had good attitudes towards the infection prevention tools and admitted to have access these tools through pharmacies and stores. Conclusion: Regardig the emerging HIV/AIDS threat and the study results, it is proposed to urgently set up some courses in universities on HIV/AIDS and practical guidelines for the personal protection against the disease and an increase of user-friendly facilities and access to written sources, books and health journals.
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P-19 Prevalence of nosocomial infections and microbial causes in Torbat heydariyeh 9dey educational and clinical hospital 2013-2014 Karim Darvishpoor, Mohammad Reza Rezaeimanesh, Mohammad Myrhsny, Hassan Ramezani, Hossein Rahmanpoor Medical Sciences University of Torbat Heydariyeh Introduction and objectives: Nosocomial infections are a major problem in modern medicine and an important cause of morbidity, length of hospital stay, and increased costs imposed on hospitals and health risks incidence and mortality are considered. This study aimed to determine the prevalence of nosocomial infections by pathogens, and infection of hospitalized patients admitted to various hospitals and treatment Torbat heydariyeh in 2013 and 2014 respectively. Materials and methods: This study is a cross - section Azfrvrdyn May 1391 to March 1392 on all patients with nosocomial infections in hospitals Torbat th January had been done. Instruments for gathering information, a standard demographic questionnaire nosocomial infection was the. Data collection, self-researcher andThrough patient's medical records were obtained. Data were analyzed using discriptive statistical methods and chi-square test by SPSS statistical software version 19. Results: the average prevalence of nosocomial infection was %0.7. The most common bacterial causes of nosocomial infection were observed in the orthopedic surgery. Most common microorganisms isolated Entrohemorrhagic Escherichia Cola (9/9%), Klebsiella (7/7%), and Gram-negative (7/7 %), enterohaemorrhagic bacteria (7 / 7 %) Vastafylvkvk Kvaklazmsbt (7/7 percent) .The highest incidence site of hospital infections was related to orthopedic surgery (33%) and pneumonia (4/26%) and the most common underlying disease with was high blood pressure. Conclusion: Prevalence of nosocomial infections was relatively high in Torbat heydariyeh 9dey hospital. In order to reduce nosocomial infections, early diagnosis of infection, continuous assessment of microorganisms by sanitation workers, especially in the orthopedic.
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P-20 Identification of vaginal Lactobacillus species from patients with fungal vaginitis and healthy individual Kharazi M, Zomorodian K, Dehghan P, Yazdani M Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan,Iran Department of Medical Parasitology and Mycology, Department of obstetrics & gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Introduction and objectives: The healthy human vagina is consisting predominantly of Lactobacillus species. Lactobacilli are 107 to 108 CFU/g of vaginal fluid in healthy premenopausal women. This may play an important role in the prevention of vaginal infections. The present study aimed to determine the presence of different species of Lactobacilli in patients with vaginal candidiasis and healthy individuals. Materials and methods: The study conducted 200 women at reproductive age recruited at a gynecology clinic located in Shiraz, Iran. Of these women, 42 women were selected as patient group and 21 age-matched asymptomatic women were also selected as control group. Following the clinical examination by a gynecologist, samples were taken from the vaginal discharges for culturing on MRS agar, gram staining, and determining of pH, The API 50 CH test kit were used to identify vaginal strains of Lactobacillus. Result: Among the 63 samples of vaginal discharge, Lactobacilli were isolated in 58 samples (88.1%), the dominant species isolated in the vaginal microflora of healthy women and patient group were L.acidophilus and L.delbrueckii. The mean lactobacilli colonies in control group were 980± (820) cfu ∕ ml and in patient group were 750± (825) cfu ∕ ml. Conclusion: To the best our knowledge there is no previous report on distribution of both vaginal Lactobacillus in healthy women and patient in Iran. These data add to the limited information exiting in Iran on the correct identity of Lactobacillus spp in the vaginal flora in patients with fungal Vaginitis and Healthy individual.
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P-21 Prevalence of TEM-1, SHV-1 genes & the patterns of susceptibility antibiotic among Klebsiella pneumonia Extended-Spectrum β-lactamases production isolated from cilinical samples obtained from university hospitals of Urmia city, 2013. Parsania S, Sohrabpour M Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran Department of Microbiology, Faculty of Sciences, Zanjan Islamic Azad University, Zanjan, Iran Introduction and objectives: Klebsiella pneumoniae is a frequent nosocomial pathogen. The gene TEM-1 is one of the common genes that produce extended spectrum β-lactamase (ESBL).SHV-1 is universally found in K. pneumonia, evolved as a chromosomal gene. Aim of this study was to determine the prevalence of SHV-1 and TEM-1 genes and the patterns of susceptibility antibiotic among K. pneumoniae ESBL production isolated from clinical samples obtained from university hospitals of urmia city 2013. Materials and methods: Totally 105 isolates were identified as K. pneumonia from clinical samples hospitals. K. pneumonia was identified using standard Methods. The ESBL status was determined by double disk diffusion test (DDDT). The DNA extraction was taken by boiling and PCR detected the samples producing ESBL. The susceptibility of the isolates to 11 different antibiotics was examined by disk diffusion test. Results: The maximum antibiotic resistances were recorded against Cefalotin (49.7%) and maximum antibiotic susceptivity were recorded aginst Imipenem (98.1%). Among 105 isolates, 38 isolates (36.19%) were found ESBL positive by DDDT. From 38 isolates ESBL positive, 31 isolates were positive for SHV-1 and TEM-1. 21.2% had SHV-1 gene alone, 11.1% had TEM-1 gene alone and 44% had both of TEM-1 and SHV-1 genes. 23.7% isolates did not show either TEM-1 or SHV-1 genes. Conclusion: The spread of ESBL K. pneumonia at the study is worrisome and usage of cephalosporins against these isolates is in-effective. Therefore, it is necessary to have continuous control of K. pneumonia.
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P-22 ESBL mediated resistance in Pseudomonas aeruginosa from university hospitals of Urmia city Parsania S, Sohrabpour M Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran Department of Microbiology, Faculty of Sciences, Zanjan Islamic Azad University, Zanjan, Iran Introduction and objectives: Pseudomonas aeruginosa is an opportunistic pathogen, which causes severe infections in burned patients. Resistance to extended spectrum cephalosporins in this organism complicates treatment of infections.The understanding of beta-lactamase identification and detection in these bacteria is very valuable. The present study was undertaken to detect the extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa by phenotypic method in urmia hospitals. Materials and methods: A total of 140 strains of Pseudomonas aeruginosa were isolated from burn wound of patients in university hospitals of urmia city.The antibacterial susceptibility testing was carried out by disk agar diffusion method.The presence of the ESBL enzyme was detected by the phenotypic confirmatory test. Results: Out of 140 isolates of Pseudomonas aeruginosa, 45(%32.14) were ESBL producers and practically many of them were resistance to multiple antibiotics. Conclusion: Noticing the increasing rate of the ESBLs Producing strains,choosing the appropriate protocol for use of antibiotics, and also avoid spreading of the resistance genes among bacteria is highly recommended.
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P-23 Antimicrobial susceptibility of Streptococcus pneumoniae isolates from nasopharynx of healthy children Baratali Sharifi, Mehrnaz Rad, Mosavi F, Ghazvini K Azadi Square, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Introduction and objectives: Streptococcus pneumonia remains a common pathogen and a leading cause of morbidity and mortality. In recent years, pneumococcal strains with high level resistance to penicillin have emerged. These strains have shown resistance to other multy antibiotics. The aim of this study was to characterize the antimicrobial susceptibility of different serotypes of Streptococcus pneumoniae which were collected from nasopharynx of healthy children under 6 years old in Mashhad, Iran. Materials and methods: A total of 260 nasopharyngeal swabs were collected from healthy children under 6 years old, attending day care centers in Mashhad, Iran.The antimicrobial resistance were determined by using disk diffusion method, according to CLSI guidelines. Serotyping of Streptococcus pneumoniae isolates was performed by multiplex PCR in Pasteur Institute of Tehran, Iran. Results: The results showed that 51 isolates were confirmed as Streptococcus pneumoniae. All of these isolates were multiple resistant. Resistance of isolates against penicillin, oxacillin, cotrimoxazole, cefotaxime, erythromycin, tetracycline, clindamycin, ceftriaxone, chloramphenicol, levofloxacin were 100%, 84.31%, 84.31%, 72.55%, 68,63%, 60.78%, 47.06%, 23.53%, 23.53%, 3.92%, respectively. All of the Streptococcus pneumoniae serotypes were sensitive to augmentin and vancomycin. Fourteen serotypes of Streptococcus pneumonia were identified among the isolates. The most predominant serotypes were as follows: 3(13.73%), 6AB (13.73%), 19A (11.76%), 18(7.4%). Conclusion: In conclusion this study clearly documents increase in the prevalence of antibiotic resistance and multiple drug resistance of Streptococcus pneumoniae in this region. All of the Streptococcus pneumoniae isolates were resistant against penicillin. Continuous surveillance of antimicrobial resistance among pneumococcal isolates in local regions, pneumococcal vaccination, and appropriate use of antibiotics are essential to control this problem
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P-24 The assessment of anti-biofilm susceptibility of methanol extract of Rumex -dentatus against Pseudomonas aeruginosa isolated from burn wound infections Maryam Pezeshki Najafabadi, Maryam Mohammadi-Sichani, Mohammad Javad Kazemi, Mohammadsadegh Shirsalimian, Majid Tavakoli Msc in microbiology, Department of biology, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran Department of microbiology, Falavarjan Branch, Islamic Azad university, Esfahan,Iran Department of biology, Ashkezar Branch, Islamic Azad University, ashkezar, Yazd, Iran PhD student, North Tehran Branch, Islamic Azad University, Tehran, Iran Lorestan Agricultural and Natural Resources Research Center, Khorramabad, lorestan, Iran Introduction and objectives: Pseudomonas aeruginosa plays a role as an agent involved in serious infections in burned patients. The extracellular polymeric substance of P. aeruginosa biofilms is an ill-defined mix of polysaccharides, nucleic acids, and proteins. Rumex dentatus is belonging to polygonaceae family. This large family is growing in many countries especially in Middle East. The aim of this present study was to assess the effect of various concentration of this extract on biofilm formation of Pseudomonas aeruginosa after 48 and 72 hours. Also bacterial effect of this extract was determined by Triphenyl Tetrazolium Chloride. Materials and methods: In this study we collected this plant from Khoramabad (Iran) in May-June 2013 and dried at room temperature for 30 days. The working extracts concentrations were 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml. 30 isolates of Pseudomonas aeruginosa from burn wound swabs were obtained from EmamMosaie-Kazem hospital in Esfahan, Iran during July 2013-March 2014. Results: The results indicated that 250 mg/ml methanol extract is more effective on biofilm of these bacteria. Conclusion: There were statistically significant correlations between anti-biofilm activity and concentration of the extracts after 48 and 72 hours. This finding suggested that methanol extract of this plant could be used as the natural anti biofilm activity for burn wound infections.
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P-25 Prevalence of Helicobacter pylori vacA allels and cagA genes in the feces of seropositive childrenin Kermanshah city Shahveh, Mohsen OmraniMahmood Social Security Organization, Kermanshah, Iran Introduction and objectives: Helicobacter pylori are an important factor digestive disease. cagA and vacA genes of H. pylori strains cause increased risk of stomach cancer. Many studies on children who have symptoms of H. pylori infection by Endoscopy indicate that more virulent strains may also have his stomach. Material and methods: In this study, 300 children aged 2 to 9 years were examined and tested for detction of anti IgG. After DNA extraction from stool, samples tested by PCR for detection of vacA genotypes including allele frequency, (s1a, s1b, s1c, s2) and (m1a, m1b, m2) and for the presence of cagA gene Results and discussion: The findings showed that most strains of H. pylori genotypes vacA s2/m2 are isolated. But the frequency of cagA gene sees in only 10 %. According to the results, the most frequent allele of the gene vacA s2/m2 this area has existing children. To ensure these wider molecular studies in other populations is recommended.
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P-26 Isolation of potentially pathogenic Vahlkpfamiids from recreational water sources in Ilam city Reza Saberi, Alireza Latifi, Samira Shahdost, Zohreh Lasgerdi, Maryam Niyyati Department of Parasitology and Mycology, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran Introduction and objectives: Free-living amoebae contain Vahlkampfiidae family. Their niches are mostly in fresh water. Vahlkamfiids contain pathogenic genera causing brain and eye disturbance. The aim of the current study was to determine the presence of Vahlkamphiidae in the recreational water sources in Ilam city. There were no previous researches in Ilam city. Material and methods: Twenty five samples were collected from surface waters of recreational centers including parks and ponds of Ilam city. Each sample was filtered through a cellulose nitrate membrane and cultured on 1 % non-nutrient agar. These plates were followed for 4 weeks and the morphological criteria were tested using page key. Result: Of 25 samples, 16 (62%) were positive for Vahlkampfiids amoebae according to appearance of cysts and trophozoites. In the positive plates wormy shaped trophozoites with size up to 40 µ and large vesicular nucleus were seen. Cystic forms were detected by their spherical shape with distinctive double walls. Their sizes were approximately 10-20 µ. Conclusion: Due to high occurrence of Vahlkampfiids amoebae in recreational water sources of Ilam city, increased public awareness and posting of warning signs or replacement of the recreational water sources at regular time should implicated.
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P-27 Study on the prevalence of Trichomonas vaginalis infection in humans referred to central laboratory of Tabriz, Iran, since April-March, 20122013 Sedigheh Sarafraz, Mohammad Fatollahzadeh, Yosef Ghadimi, Niloufar Akbari, Mahdi Parsaei, Vahid Rahnamaye Hayati Hagh Tabriz University of Medical Sciences, Central Laboratory of Tabriz Introduction and objectives: Trichomonasis is a unicellular infection in the lower urinary tract-genitals in men and women that usually occurs during the reproductive years.Pretty much way of its transition is through sexual contact. .Diagnosis of Trichomonasis is a warning to the other sexually transmitted diseases that can simultaneously exist. Thus it is necessary to identify individuals who have the symptoms. As a result, this study was to investigate the prevalence of Trichomonasvaginalis in patients with symptoms of urinary tract infections, which most of them had burning and frequent urination. Materials and methods: Methods in this study, 49000 urine samples were collected from men and women admitted to the Tabriz central laboratory during 24. In this study, the patients were asked to a morning urine samples.After centrifugation and preparation of urine sediment examined microscopic examination of samples immediately. Results: In this study, the following results were achieved 57 cases (0.11 %) were positive forTrophozoiteTrichomonasvaginalis, that positive cases among women were 42 cases (0.08 %) which 35 cases were low-literacy and Suburbs, that The highest prevalence was at ages 20-38. positive cases among men were 15 cases (0.03 %) which 8 cases were low-literacy and Suburbs, that The highest prevalence was at ages 25-52. According to a survey of patients, mostly people with lowliteracy andrural areashad thehighest prevalence. The most prevalent was in the summer and fall season (OctoberSeptember). Conclusion: Sincethe correct diagnoses can helptreatment. Therefore, accurate diagnosis and the results obtained, Prevalence of the disease accounted for a large percentage among people with low education and those living in the suburbs.Thusthe inclusion oftraining incommunity health centerswill control andplay a rolein disease prevention.
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P-28 Contamination of tap water sources to Acanthamoeba in Ahvaz city, Iran Roya Salehi, Maryam Niyyati, Samira Dodanghe, Zohreh Lasjerdi Department of Medical Parasitology and Mycology, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran Introduction and objectives: Acanthamoeba spp. is the causative agent of severe diseases such as keratitis and encephalitis. Presence of Acanthamoeba spp. in environmental sources such as tap water could be a health hazard for high-risk people including contact lens wearers and immunosuppressed patients.The present study was conducted to address the occurence of Acanthamoeba spp. in tapwater sources and filters of Ahvaz city, south of Iran. Materials and methods: Fifty Samples were collected from tap water sources and water filters in Ahvaz city. Filtration and cultivation of samples was performed using non-nutrient agar. All plates were sealed, incubated at 30°C, and monitored daily for outgrowth of Acanthamoeba. The presence of Acanthamoeba was confirmed by cyst and trophozoite morphology. For further evaluation and for elimination of bacterial and fungal contamination, the cysts were cloned, and subcultured by cutting out a small piece of agar and placing it onto a fresh plate. Result: Out of 50 water samples, 16% were positive for Acanthamoeba trophozoites and cysts according to morphological criteria and page key.Positive plates contained Acanthamoeba spp. with flat shaped trophozoites ranging from 15- 30 µ with obvious acanthopodiaand double walled cyst with star or polygonal shaped endocysts. The cysts were in clump and their size was around 10-16 µ. Conclusion: Presence of Acanthamoeba in tap water sources and filters in Ahvaz city is of concern for high risk people. More improved filtration and disinfection procedures are of utmost importance to prevent Acanthamoeba related infections.
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P-29 Identification of Acinetobacter baumannii using ompA and csuE genes by multiplex PCR Leila Saeedi, Fatemeh Haddadi, Hossein Kamaladini, Jalal Mardaneh Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran Introduction and objectives: Acinetobacter baumannii is important hospital pathogen which especially affects the patients with an acute condition. Special ability of the bacteria to survive on different surfaces of hospitals and obtaining antibiotic resistance is a major clinical challenge. Hence due to the importance of the bacteria, its rapid detection is essential. Specific genes of Acinetobacter including csuE gene is essential for biofilm formation and ompA gene induces apoptosis in epithelial cells. In this study two specific primer pairs were designed and Acinetobacter baumannii strain ATCC 19606 was identified using Multiplex PCR. This study was conducted for further detection of this bacterium in clinical specimens by gold nanoparticles. Materials and methods: Acinetobacter baumannii strain ATCC 19606 was kindly provided by Clinical Microbiology Research Center, Shiraz. The bacteria were cultured on the mueller hinton agar (MHA) medium and DNA was extracted using boiling method. In the next step, for identification of the bacteria, Multiplex PCR was performed using specific primers of ompA and csuE genes. Results: Results showed expected bands of 299 bp and 105 bp corresponded to ompA and csuE genes respectively. Conclusion: Following identification of Acinetobacter baumannii with Multiplex PCR, the results could be applied for further detection of the bacteria in clinical specimens by gold nanoparticle probes.
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P-30 Evaluation of serum antibodies to hepatitis B virus recombinant vaccines health workers vaccinated against hepatitis B in Shahid Chamran hospital, Dasht–ea-azadgan Heidari S, Reza Tofighi SE, Mohammad Roayaei Ardakani, Akhond MR MS of Microbiology, Department of Biology, Faculty of Science, Shahid ChamranUniversity of Ahvaz ,Assistant Professor Section of Microbiology, Department of Biology, Faculty of Science Shahid Chamran University of Ahvaz ,Assistant Professor Section of Microbiology, Department of Biology, Faculty of Science Shahid Chamran University of Ahvaz Shahid ,Assistant Professor Section of Statistics, Department of Statistics, Faculty of Science Chamran University of Ahvaz Introduction and objectives: Hepatitis B virus (HBV) B is a major cause of acute and chronic hepatitis, which can even lead to cirrhosis, and hepatocellular carcinoma is (3). Hospitals compared with community groups due to repeated exposure to patients and blood products in are at higher risk of infection with hepatitis B (1). Of prevention, immunization by vaccination against these diseases. The aim of this study was to evaluate immunogenicity of recombinant hepatitis B vaccine immune response and the shahid Chamran Hospital Health workers (1). Materials and methods: This cross-sectional study of 130 hospital personnel shahid Chamran recombinant hepatitis B vaccine was received. Blood samples were taken from different levels of antibody against hepatitis B were evaluated by ELISA. Results: The results were 17/26% good response personnel (MIU
