A Novel Propane Monooxygenase Initiating ... - ACS Publications

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Dec 1, 2017 - THF MOs are responsible for inserting a hydroxyl group at theα- ..... (5) Adamson, D. T.; Mahendra, S.; Walker, K. L.; Rauch, S. R.;. Sengupta, S.
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Letter Cite This: Environ. Sci. Technol. Lett. 2018, 5, 86−91

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A Novel Propane Monooxygenase Initiating Degradation of 1,4Dioxane by Mycobacterium dioxanotrophicus PH-06 Daiyong Deng, Fei Li, and Mengyan Li* Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, New Jersey 07102, United States S Supporting Information *

ABSTRACT: Monitored natural attenuation and bioremediation are cost-efficient and eco-friendly approaches to mitigating prevalent 1,4-dioxane (dioxane) plumes. Unfortunately, their field applications have been greatly undermined given our scarce knowledge of the diversity of dioxane biodegradation pathways and associated key enzymes. At present, only tetrahydrofuran monooxygenases (THF MOs) are known to initiate dioxane degradation in dioxane metabolizers. In this study, we deciphered the essential catalytic role of a novel propane MO (encoded by the prmABCD gene cluster) in dioxane metabolism by Mycobacterium dioxanotrophicus PH-06. This propane MO is phylogenetically distinct from THF MOs on the basis of the low levels of amino acid sequence identity (