Journal of Chromatographic Science, 2016, Vol. 54, No. 4, 561–568 doi: 10.1093/chromsci/bmv187 Advance Access Publication Date: 19 December 2015 Article
Article
A Simple, Rapid and Reliable Method to Determine Imipramine and Desipramine in Mouse Serum Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Time-ofFlight Mass Spectrometry Jing Zhao1, Yujin Shin1, Kwang-Hoon Chun2, Hye-Ran Yoon3, and Jeongmi Lee1,* 1
School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea, 2Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea, and 3College of Pharmacy, Duksung Women’s University, Seoul 01369, Republic of Korea
*Author to whom correspondence should be addressed. Email:
[email protected] Received 17 September 2014; Revised 28 August 2015
Abstract A rapid and sensitive ultra-high-performance liquid chromatography–quadrupole-time-of-flight mass spectrometric (UHPLC–Q-TOF-MS) method was developed for quantification of imipramine, one of the most widely used tricyclic antidepressants, and desipramine, an active metabolite of imipramine, in mouse serum. The developed method included a simple protein precipitation with acetonitrile in 50 μL of serum and analyte separation on an Acquity UPLC BEH C18 column using a gradient elution of acetonitrile with 0.1% formic acid and 20 mM ammonium formate. As a result, the entire analysis time was
