... is extremely selective, requires only a single dispensing step on a stan- .... Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, ...
ABSOLUTE QUANTIFICATION OF MICRORNA FROM HUMAN AND MOUSE TISSUE RNA USING HIGHLY SELECTIVE ISOTACHOPHORETIC FOCUSING Alexandre Persat1, Raghu R. Chivukula2, Joshua T. Mendell2 and Juan G. Santiago1* 1 2
Stanford University, U.S.A. and
Johns Hopkins University, U.S.A
ABSTRACT We present a novel technique for the quantification of small, non-coding RNA using selective isotachophoretic preconcentration and focusing. Our technique allows for absolute quantification of RNA shorter than 60 nt, in the size range of microRNA (miRNA). Our quantitation is extremely selective, requires only a single dispensing step on a standard chip, and quantifies oligomers
