TH
12 INTERNATIONAL CONFERENCE PAKISTAN SOCIETY OF BIOCHEMISTRY AND MOLECULAR BIOLOGY (PSBMB) THE ERA OF OMICS: CHALLENGES & SOLUTIONS DECEMBER 4-7, 2014, BAHAWALPUR, PAKISTAN
Abstract Submission Form Title: HPLC assay of caffeine and paraxanthine for CYP1A2 phenotype assessment Authors: Ayesha Razaq1, Shagufta Kamal2*, Shumaila Kiran2, Ismat Bibi3, Nosheen Aslam2, Bushra Munir2 Amna Kamal4 and Jeveria Rehman1 Corresponding Author: …
[email protected]:
[email protected] Authors Affiliations1Department of Chemistry, Government College University, Faisalabad 38000, Pakistan. 2
Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad 38000, Pakistan. 3
Department of Chemistry, Islamia University, Bahawalpur 63100, Pakistan.
4
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38000, Pakistan.
Abstract: (maximum 250 words, attach separate sheet if required) Presentation:
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Oral
Poster
Caffeine, not cancer causing agent or not decreases the risk of cancer but used just to note the activity of Cytochrome P-450 1A2 by converting into its metabolite i.e., paraxanthine. The purpose of present study was to determine the caffeine and its metabolite phenotypes and their relation to cancer risk in healthy female volunteers of local population in Pakistan. The average value of metabolic ratio was found to be 1.182995 ± 0.21137. BMI of all volunteers were found to be 19.93Kg/m2. Retention time was 15 and 37mins for 17X and 137X respectively. The linearity of calibration curve of 137X and 17X were covered 0 – 12 µg/ml (R2 = 0.994). A significant positive correlation was observed between metabolic ratio and cancer risk factors. We could conclude that all the volunteers are fast metabolizers having greater risk of cancerous diseases. Send Filled Form to:
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