abstracts of the ninth international conference of

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ANTICANCER RESEARCH 34: 5761-6258 (2014)

ABSTRACTS OF THE NINTH INTERNATIONAL CONFERENCE OF ANTICANCER RESEARCH October 6-10, 2014 Sithonia, Greece Under the Auspices of The Union for International Cancer Control (UICC) The Asian and Pacific Federation of Clinical Biochemistry (APFCB) The Asian Pacific Organization for Cancer Prevention (APOCP) The Austrian Society for Radiation Oncology (ÖGRO) The European Group on Tumor Markers (EGTM) The Hungarian Society of Epidemiology The International Cancer Microenvironment Society (ICMS) The International Geriatric Radiotherapy Group (IGRG) The International Institute of Anticancer Research (IIAR) The International Society for Biological and Environmental Repositories (ISBER) The International Society of Oncology and Biomarkers (ISOBM) The Italian Society of Uro-Oncology (SIUrO) The Latinamerican and Caribbean Society of Medical Oncology (SLACOM) The Lithuanian Society of Radiation Oncology (LSRT) The North-Eastern German Society of Gynecological Oncology (NOGGO) The Polish Society of Radiation Oncology (PSTRO) The Portuguese Society of Radiotherapy - Oncology (SPRO) The Society of Biotherapeutic Approaches, Japan The Szeged Foundation for Cancer Research, Hungary The Turkish Society for Electron Microscopy (TSEM)

Supported by The International Institute of Anticancer Research

Organizing Committee

P.A. Abrahamsson, Department of Urology, Lund University, Malmö, Sweden N. Agnantis, Department of Pathology, University of Ioannina Medical School, Ioannina, Greece F.E. Ahmed, GEM Tox Labs, Institute for Research in Biotechnology, Greenville, NC, USA C. Alexiou, Division Experimental Oncology/Nanomedicine, Hals-Nasen-Ohren-Klinik/ENT-Clinic, Universitätsklinikum Erlangen, Erlangen, Germany L. Amaral, Institute of Hygiene & Tropical Medicine, Universidade Nova de Lisboa, Lisbon, Portugal F.L. Ampil, Department of Radiology, Louisiana State University Health Sciences Center in Shreveport, Shreveport, LA, USA R. Andersson, Department of Surgery, Faculty of Medicine, Lund University, Lund, Sweden S. Arbak, Department of Histology and Embryology, Acibadem University School of Medicine, Istanbul, Turkey C. Aukim-Hastie, Faculty of Health & Medical Sciences, University of Surrey, Guildford, Surrey, UK V. Barak, Immunology Laboratory for Tumor Diagnosis and Hematology Department, Hadassah - Hebrew University Medical Center, Jerusalem, Israel D.-T. Bau, Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan G. Bauer, Institute of Virology, Department of Medical Microbiology and Hygiene, University Medical Center, Freiburg, Germany F. Betsou, Integrated BioBank of Luxembourg (IBBL), Luxembourg A. Bishayee, Department of Pharmaceutical Sciences, School of Pharmacy, American University of Health Sciences, Signal Hill, CA, USA R. Bjerkvig, Department of Biomedicine, University of Bergen, Bergen, Norway G. Broich, Coordinatore Sanitario Regionale, Gruppo Policlinico di Monza, Monza, Italy C. Carlberg, Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland J. Carlsson, Unit of Biomedical Radiation Sciences, Dept. of Radiology, Oncology and Radiation Science, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden D. Characiejus, Deparment of Pathophysiology and Immunology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania C.J. Chen, Graduate Institute of Epidemiology and Preventive Medicine, National Taiwan University, Taipei, Taiwan G. Ciarimboli, Experimental Nephrology, Medical Clinic D, Münster University Hospital, Münster, Germany B.F.C. Clark, Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark R. Clarke, Department of Oncology, Georgetown University Medical Center, Washington, DC, USA P. Collery, Society for the Coordination of Therapeutic Researches, Algajola, France G. Conti, Department of Urology, St. Anna Hospital, Como, Italy H. Cross, Department of Pathophysiology, Medical University of Vienna, Vienna, Austria M. De Braekeleer, Laboratoire de Cytogénétique, Faculté de Médecine et des Sciences de la Santé, Université de Bretagne Occidentale, Brest, France K. De Meirleir, Himmunitas Foundation, Brussels, Belgium A.Z. Dudek, Department of Hematology/Oncology, University of Illinois, College of Medicine at Chicago, Chicago, IL, USA A. Dutour, Department of Clinical Sciences, Centre de Recherche en Cancérologie UMR Inserm U1052, Lyon, France P. Dziegiel, Department of Histology and Embryology, Medical University of Wroclaw, Wroclaw, Poland T. Efferth, Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany W. Engström, Department of Biomedical Sciences and Veterinary Public Health. Faculty of Veterinary Medicine, SLU, Uppsala, Sweden M. Eskelinen, Department of Surgery, Kuopio University Hospital, Kuopio, Finland J.A. Fernandez-Pol, Antagoras Agrobusiness, LLC, Chesterfield, MO, USA A. Fields, Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, FL, USA M. Friedrich, Department of Gynecology and Obstetrics, HELIOS-Klinikum Krefeld, Krefeld, Germany R. Friedrich, Department of Oral & Maxillofacial Surgery, Eppendorf University Hospital, Hamburg, Germany H.-J. Gabius, Department of Physiological Chemistry, Veterinary Sciences Department of Veterinary Medicine Faculty of the Ludwig-Maximilians-University, Munich, Germany R. Ganapathi, Levine Cancer Institute, Carolinas HealthCare System, Charlotte, NC, USA A.F. Gazdar, The Hamon Center for Therapeutic Oncology Research, U.T. Southwestern Medical Center at Dallas, Dallas, TX, USA V. Gebbia, Medical Oncology Unit, Dipartimento Oncologico, La Maddalena, Università degli Studi, Palermo, Italy

ANTICANCER RESEARCH 34: 5761-6258 (2014)

J.W. Greiner, Laboratory of Tumor Immunology & Biology, CCR, NCI, Bethesda, MD, USA F.E. Guadagni, SR Research Center, IRCCS San Raffaele Pisana, Rome, Italy J. Hau, Department of Experimental Medicine, University of Copenhagen, Copenhagen, Denmark R. Hoffman, AntiCancer Inc., San Diego, CA, USA S. Holdenrieder, Institut für Klinische Chemie und Klinische Pharmakologie-Zentrallabor-Universitätsklinikum Bonn, Bonn, Germany T. Isbir, Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey S. Jhanwar, Memorial Sloan Kettering Cancer Center, Laboratory of Solid Tumor Genetics, New York, NY, USA M. Jhanwar-Uniyal, Departmenet of Neurosurgery, New York Medical College, Valhalla, NY, USA B. Kaina, Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Mainz, Germany E. Kandil, Department of Surgery, Tulane University School of Medicine, New Orleans, LA, USA Y. Keisari, Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, Israel D.G. Kieback, Klinikum Vest, Standort Marl, Marl, Germany I. Kiss, Institute of Preventive Medicine, University of Pecs Medical School, Pécs, Hungary R. Klapdor, Internal Unit, ZeTDT GmbH, Hamburg, Germany R. Knecht, Department of Otorhinolaryngology, Head and Neck Surgery and Oncology, University Medical Center HamburgEppendorf, Hamburg, Germany M. Kohonen-Corish, Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, NSW, Australia M. Koutsilieris, Laboratory of Experimental Physiology, Department of Basic Sciences, University of Athens Medical School, Athens, Greece K. Kubota, Second Department of Surgery, Dokkyo Medical University, Mibu, Tochigi, Japan P.M. Kumar, Division of Laboratory & Regenerative Medicine, University of Manchester Medical School, Manchester, UK S. Kumar, Department of Biological Sciences, Manchester Metropolitan University, Manchester, UK O.D. Laerum, Department of Pathology & Oncology, Institute of Clinical Research, Haukeland University Hospital, Bergen, Norway S.A. Lamprecht, Division of Clinical Biochemistry and Pharmacology, Ben-Gurion University of the Negev, Beer-Sheva, Israel W. Lichtenegger, Charité Campus Virchow-Klinikum, Klinik für Frauenheilkunde und Geburtshilfe, Berlin, Germany D. Lincoln, Independent Research Foundation, Chapel Hill, QLD, Australia B. Littlefield, Eisai Inc., Andover, MA, USA F. Lumachi, University of Padua, School of Medicine, Padova, Italy G. Martorana, Department of Urology, University of Bologna, Bologna, Italy S. Mitra, Department of Radiation Oncology, Houston Methodist Research Institute, Galveston, TX, USA R. Molina, Laboratory of Biochemistry (Oncobiology Unit); Thorax Institute Hospital Clínic, IDIBAPS, Barcelona, Spain J. Molnar, Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary R. Morgan, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK N. Motohashi, Department of Medicinal Chemistry, Meiji Pharmaceutical University, Tokyo, Japan M. Mueller, Department Biomedical Engineering, Faculty of Medical and Life Sciences, Hochschule Furtwangen University, Villingen-Schwenningen, Germany F.M. Muggia, New York University School of Medicine and NYU Cancer Institute, New York, NY, USA R.M. Nagler, Department of Oral and Maxillofacial Surgery and Oral Biochemistry Laboratory, Rambam Medical Center and Rappaport Faculty of Medicine, Israel Institute of Technology-Technion, Haifa, Israel R. Nano, Dipartimento Biologia e Biotecnologie "Lazzaro Spallanzani", Università degli Studi di Pavia, Pavia, Italy N. Nguyen, Department of Radiation Oncology, Howard University, Tucson, AZ, USA C. Nicolini, Laboratories of Biophysics and Nanobiotechnology, Department of Experimental Medicine, University of Genova, Genoa, Italy C. Pace-Asciak, The Hospital for Sick Children Research Institute, Toronto, ON, Canada S. Pathak, Department of Genetics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA G.J. Pilkington, Cellular and Molecular Neuro-oncology Research Group, University of Portsmouth, Portsmouth, UK C.D. Platsoucas, College of Sciences, Old Dominion University, Norfolk, VA, USA A. Polliack, Lymphoma - Leukemia Unit, Department of Hematology, Hadassah University Hospital, Jerusalem, Israel J. Reichrath, Klinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany D.A. Ribeiro, Department of Pathology, Paulista Medical School, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil 5764

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece M. Rigaud, ASTRALAB, Clinical Laboratory, Limoges, France F.M. Robertson, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA M. Roselli, Department of Systems Medicine, Medical Oncology Unit, Tor Vergata Clinical Center, University of Rome “Tor Vergata", Rome, Italy G.R. Rutteman, University Clinic of Companion Animals, Utrecht and Veterinary Specialist Center De Wagenrenk, Wageningen, Netherlands C. Sansom, Department of Biological Sciences, Birkbeck, University of London, London, UK S. Sarkar, Cancer Center, Department of Medicine, Boston University School of Medicine, Boston, MA, USA A. Schauer, Zentrum Pathologie, Georg-August-Universitat-Gottingen, Göttingen, Germany D. Schiffer, Neuro-bio-oncology Center, Policlinico di Monza Foundation, Vercelli, Italy J. Schneider, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Alcorcón (Madrid), Spain M. Schneider, FB C − Organische Chemie, Bergische Universität Wuppertal, Wuppertal, Germany O.J. Semmes, Leroy T. Canoles, Jr. Cancer Research Center, Eastern Virginia Medical School, Norfolk, VA, USA A. Seth, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada G. Sica, Institute of Histology and Embryology, “A. Gemelli” Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy J. Slansky, Integrated Department of Immunology, School of Medicine, University of Colorado Denver, Denver, CO, USA K. Smetana, 1st Faculty of Medicine, Institute of Anatomy, Charles University, Prague, Czech Republic S.S. Smith, NanoLab, Division of Urology, City of Hope Comprehensive Cancer Center, Duarte, CA, USA R.M. Snapka, Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA D.D. Spyropoulos, Pathology & Lab Medicine Walton, Medical University of South Carolina, Charleston, SC, USA J. Sterling, Center for Bone Biology, Department of Medicine, Vanderbilt University, Nashville, TN, USA T. Stigbrand, Department of Immunology and Immnunochemistry, Umeå University, Umeå, Sweden K. Syrjänen, Department of Clinical Research, Biohit Oyj, Helsinki, Finland R.T. Todorova, IBPhBME-BAS, Sofia, Bulgaria O. Topolcan, Department of Immunoanalysis, Faculty Hospital in Pilsen, Charles University, Pilsen, Czech Republic G.C. Torre, Centro Ippocrate, Finale Ligure (SV), Italy V. Treska, Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic M. de L.G. Trigo, Serviço de Braquiterapia, Instituto Português Oncologia Porto FG, EPE, Porto, Portugal G. Tsangaris, Central Unit of Genomics-Proteomics, Foundation of Biomedical Research of the Academy of Athens, Athens, Greece E. Vairaktraris, Clinic for Maxillofacial Surgery, “Attikon” University Hospital, Athens, Greece D. Vesely, Department of Medicine, James A. Haley VA Medical Center and University of South Florida Morsani Health Sciences Center, Tampa, FL, USA D. Vetvicka, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic J.K. Vishwanatha, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA G. Vollmer, Department of Biology, Technische Universitaet Dresden, Dresden, Germany A. Wani, Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA I.P. Witz, Cancer Microenvironment, Tel-Aviv University, Tel Aviv, Israel Y. Yen, Department of Molecular Pharmacology, City of Hope Comprehensive Cancer Center, Duarte, CA, USA C.H. Yip, Breast Centre, Subang Jaya Medical Centre, Selangor, Malaysia O. Zoras, Department of Surgical Oncology, University Hospital of Heraklion, Heraklion, Greece

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Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece

List of Participants

Abdel-Mageed A.B., Tulane University School of Medicine, New Olreans, LA, USA Abderrahim H., Qatar Biobank, Qatar Foundation, Doha, Qatar Abu Freha N., Soroka Medical Center, Beer-Sheva, Israel Abdi E., Medical Oncology, The Tweed Hospital, Powell Street, Tweed Heads, NSW, Australia Aggarwal B., Dept. of Experimental Therapeutics, Univ. of Texas M. D. Anderson Cancer Center, Houston, TX, USA Agnantis N., Department of Pathology, University of Ioannina Medical School, Ioannina, Greece Aguilar-Lemarroy A., División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO)-Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico Ahmed F.E., GEM Tox Labs, Greenville, NC, USA Ahmed M., Department of Research Oncology, King's College London, London, UK Ährlund-Richter L., Karolinska Institutet, Dept. of Women's and Children's Health, Division of Paediatric Oncology, Stockholm, Sweden Aiba H., Department of Orthopaedic Surgery, Nagoya City University, Nagoya-city, Japan Akbal O., Gazi University, Polatlı Art and Science Faculty, Biology Department, Polatlı, Ankara, Turkey Akimov M.G., Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia Alaiya A.A., Proteomics Unit, Stem Cell Therapy Program, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia Alaoui-Jamali M., Depts of Medicine and Oncology, Faculty of Medicine, McGill University, Montreal, Canada Alexandrova A.Y., N.N. Blokhin Russian Cancer Res. Center of the Russian Academy of Medical Sciences, Moscow, Russia Al-Khanbashi M., Sultan Qaboos University, Muscat, Oman Allegrucci C., SVMS, Sutton Bonington Campus, University of Nottingham, Loughborough, UK Allen D., BBL (Integrated BioBank of Luxembourg), For Next Generation Healthcare, Luxembourg Alpizar-Alpizar W., Centre for Research on Microscopic Structures (CIEMic), Univ. of Costa Rica, San José, Costa Rica Althurwi S., Discipline of Biomedical Science, Sydney Medical School, University of Sydney, Lidcombe, NSW, Australia Alvarado A., Decanato de Ciencias Veterinarias Universidad Centroccidental “Lisandro Alvarado”, Lara, Venezuela Amaral L., Travel Medicine of the Center for Malaria and Tropical Diseases (CMDT), Institute of Hygiene and Tropical Medicine, Universidade Nova de Lisboa, Lisbon, Portugal Amemiya Y., Biological Sciences, Sunnybrook Research Institute, Toronto, ON, Canada Ammoun S., Plymouth University, Peninsula Sch. of Medicine and Dentistry, The Inst. of Translational and Stratified Medicine, Plymouth, UK Amrani Hassani Joutei H., Département de Biologie Moléculaire, Centre spécialisé Anoual, Casablanca, Maroc Anastassopoulou J., National Technical University of Athens, Radiation Chemistry and Biospectroscopy, Athens, Greece Anderson D., School of Life Sciences, University of Bradford, Bradford, UK. Anderson S.M., Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA Andersson R., Department of Surgery, Clinical Sciences Lund, Skåne Univ. Hospital and Lund University, Lund, Sweden Angelov K., Department of Surgery, University Hospital “Aleksandrovska”, Sofia, Bulgaria Anner P., Dept. of Biomedical Imaging and Image-guided Therapy, Division of Nucl. Medicine, Medical Univ. of Vienna, Vienna, Austria Annovazzi L., Neuro-Bio-Oncology Center, Policlinico di Monza Foundation (Vercelli) / Consorzio di Neuroscienze, Univ. of Pavia, Vercelli, Italy Antonarakis S.E., Dept. of Genetic Medicine and Development, Univ. of Geneva Medical School, Geneva, Switzerland Antov G.G., Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria Aoki T., Respiratory Division, Dept. of Internal Medicine, Tokai Univ. School of Medicine, Isehara, Kanagawa, Japan Apaydin E., Department of Biotechnology, Anadolu University, Life Sciences Institute, Eskişehir, Turkey Arbak S., Department of Histology and Embryology, Acibadem University School of Medicine, Istanbul, Turkey Arfaoui A., Service de Biologie Clinique, Institut Salah Azaiz, Tunis, Tunisie Arimondo P.B., USR CNRS-Pierre Fabre n°3388, CRDPF, Toulouse, France 5767

ANTICANCER RESEARCH 34: 5761-6258 (2014)

Arosio P., Dipartimento di Fisica, Università degli Studi di Milano, Milano, Italy Arrighi N., Unit of Urology, “Città di Brescia” Clinical Institute-San Donato Hospital Group, Milano, Italy Arsenopoulou V., Laboratory of Physiology, Dept. of Medicine, Sch. of Health Sciences, Univ. of Thessaly, Larissa, Greece Atanasov A.T., Medical Faculty, Department of Physics and Biophysics, Trakia University, Stara Zagora, Bulgaria Atanasova S., Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria Auge J.M., Biochemistry and Molecular Genetics Department, Hospital Clínic, Barcelona, Spain Augsten M., Department of Oncology-Pathology, CCK, Karolinska Institutet, Stockholm, Sweden Aukim-Hastie C., Faculty of Health & Medical Sciences, University of Surrey, Surrey, UK Bacalbasa N., "Carol Davila", University of Medicine and Pharmacy, Bucharest, Romania Bai Y., Centre for Cancer Prevention and Treatment, Lanzhou University, Lanzhou, Gansu, PR China Bajenova O., Theodosius Dobzhansky Center for Genome Bioinformatics at St. Petersburg State Univ., St. Petersburg, Russia Bakalova R., Medical Faculty, Sofia University, Sofia, Bulgaria Baker B.J., Departments of Chemistry, University of South Florida, Tampa, FL, USA Banerji Prasanta, Homeopathic Research Foundation (PBHRF), Kolkata, West Bengal, India Banerji Pratip, Homeopathic Research Foundation (PBHRF), Kolkata, West Bengal, India Bar-Shalom R., Dept of Human Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, Haifa, Israel Barak V., Immunology Laboratory for Tumor Diagnosis, Oncology Dept., Hadassah- Hebrew Univ. Medical Center, Jerusalem, Israel Batistatou A., Department of Pathology, Ioannina University Medical School, Ioannina, Greece Bau D.-T., Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC Bellini M.F., Molecular Biology and Cytogenetic Laboratory, USC, Universidade do Sagrado Coração, Bauru, SP, Brazil Bendas G., Pharmaceutical Institute, University Bonn, Bonn, Germany Benericetti E., Consulente Neurochirurgo Senior presso Gruppo Villa Maria GVM, Lugo Ravenna, Italy Bernasconi M., Department of Oncology, University Children’s Hospital, Zurich, Switzerland Berte N., Department of Toxicology, University Medical Center Mainz, Mainz, Germany Betsou F., BBL (Integrated BioBank of Luxembourg), For Next Generation Healthcare, Luxembourg Bhakat K.K., Dept. of Genetics Cell Biology and Anatomy, Univ. of Nebraska Medical Center, Omaha, NE, USA Bhandari P., Worldfriendship Nepal-Ngo, Maharajgunj-03, Kathmandu, Nepal Bhaumik S.R., Dept. of Biochemistry and Mol. Biology, Southern Illinois Univ. School of Medicine, Carbondale, IL, USA Bhushan A., Dept. of Pharmaceutical Sciences, Jefferson Sch. of Pharmacy, Thomas Jefferson Univ., Philadelphia, PA, USA Bielecka A.M., Department of Pharmacology, Medical University of Silesia, Katowice, Poland Bireller E.S., Istanbul University, Inst. of Experimental Medical Research, Dept. of Mol. Medicine, Capa, Istanbul, Turkey Bjerkvig R., NorLux Neuro-Oncology, Dept. of Biomedicine, University of Bergen, Bergen, Norway Black J.D., Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE, USA Bölke E., Department of Radiation Oncology, Heinrich Heine University, Duesseldorf, Germany Bollito E.R., Pathological Anatomy, San Luigi Gonzaga Hospital, University of Turin, Orbassano, Turin, Italy Bønsdorff T.B., Oncoinvent AS, Oslo, Norway Bonucci M., Surgical Pathology Department, Outpatient Laboratory Oncology, San Feliciano Hospital, Rome, Italy Boren T., Department Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden Bose S.R., Department of Radiation Oncology, Howard University, Washington, DC, USA Bousquet G., Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Pathologie, UMR-S 1165, Paris, France Braczkowski R.S., Department of Public Health, Silesian Medical University, Bytom, Poland Braidwood L., Virttu Biologics Ltd, Dept of Neurology, Southern General Hospital, Glasgow, UK Branca J.J.V., Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italy Bray L., Leibniz Institute for Polymer Research, Max Bergmann Center of Biomaterials, Dresden, Germany Bremer E., Translational Surgical Oncology, Dept. of Surgery, Univ. Medical Center Groningen, University of Groningen, Groningen, the Netherlands Brenner W., Department of Urology, Johannes Gutenberg University Medical Center, Mainz, Germany Briasoulis E., Cancer Biobank Center, University of Ioannina, Ioannina, Greece 5768

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Brodin O., Department of Oncology, Karolinska University Hospital, Stockholm, Sweden Broich G., Coordinatore Sanitario Regionale, Gruppo Policlinico di Monza, Monza, Italy Bryukhovetskiy A.S., NeuroVita Clinic of Restorative Interventional Therapy and Neurology, Moscow, Russia Bryukhovetskiy I.S., Lab. of Molecular and Cellular Neurobiology of the Sch. of Biomedicine of the Far Eastern Federal Univ. of Vladivostok, Vladivostok, Russia Bugaytsova J., Department Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden Buondonno I., Department of Oncology, University of Torino, Torino, Italy Bureau E., Centre for Therapeutics Discovery, MRC Technology, Mill Hill, London, UK Butkiewicz D., Center for Transl. Research and Mol. Biology of Cancer, M. Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland Capelli E., Dept. of Earth and Environmental Sciences, Laboratory of Immunology and Genetic Analysis, Univ. of Pavia, Pavia, Italy Carlsson J., Dept of Radiology, Oncology and Radiation Science, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden Carvalho, M.I., Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Catalan-Diaz M., Clinical and Mol. Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile Ceausu A.R., Dept. of Microscopic Morphology/Histology, Angiogenesis Research Center, “Victor Babes” Univ. of Medicine and Pharmacy, Timisoara, Romania Červinková B., Dept. of Analytical Chemistry, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic Chambers T., Dept. of Biochemistry and Molecular Biology, Univ. of Arkansas for Medical Sciences, Little Rock, AR, USA Champeris-Tsaniras S., Department of Physiology, Medical School, University of Patras, Rio, Patras, Greece Chang Y.-S., Chang Gung Molecular Medicine Research Center, Kwei-Shan Tao-Yuan, Taiwan, ROC Chang W.-S., Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC Characiejus D, Faculty of Medicine, Vilnius University, Vilnius, Lithuania Charles R.-P., University of California, Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA Chechlinska M., Dept. of Immunolgy, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. of Oncology, Warsaw, Poland Check J.H., Cooper Medical School of Rowan University, Camden, NJ, USA Chen C.-J., Academia Sinica and Graduate Inst. of Epidemiology and Preventive Medicine, Nat.Taiwan University, Taipei, Taiwan, ROC Chen Y.-L., Institute of Oral Medicine, National Chen Kung University Medicine College, Tainan, Taiwan, ROC Chen Z.-F., State Key Lab. Cultivation Base for the Chemistry and Mol. Engineering of Med. Resources, Sch. of Chemistry and Pharmacy, Guangxi Normal Univ., Guilin, PR China Chi A., Department of Radiation Oncology, West Virginia University, Morgantown, WV, USA Chiariello M., Istituto di Fisiologia Clinica, Sede di Siena, Consiglio Nazionale delle Ricerche (CNR) and Core Research Laboratory, Istituto Toscano Tumori (ITT), AOU Senese, Siena, Italy Chien S.-S., Taichung Armed Forces General Hospital, Taichung, Taiwan, ROC Cho C.H., Sch. of Biomedical Sciences, Faculty of Medicine, The Chinese Univ. of Hong Kong, Shatin, N.T., Hong Kong, PR China Ciafré S.A., Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy Ciarimboli G., Interdisciplinary Center for Clinical Research (IZKF), University Hospital Münster, Münster, Germany Cimpean A.M., Dept. of Microscopic Morphology/Histology, Angiogenesis Res. Center, “Victor Babes” Univ. of Medicine and Pharmacy, Timisoara, Romania Ciortea R., “Iuliu Hatieganu” Univ. of Medicine and Pharmacy Cluj-Napoca, Dept. of Obstetrics and Gynecology, Cluj-Napoca, Romania Cirimbei C., "Carol Davila" Univ. of Medicine and Pharmacy, Surgery Dept. No. 1, Oncologic Inst., Bucharest, Romania Clarke R., Georgetown-Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA Collery P., Society for the Coordination of Therapeutic Researches, Algajola, France Conti G.N, Ospedale Sant’Anna, Como, Italy Coosemans A., Department of Oncology, Leuven Cancer Institute, KU Leuven, Leuven, Belgium Cotter T., Department of Biochemistry, University College Cork, Cork, Ireland 5769

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Csonka A., Inst. of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary Curti V., University of Pavia, Dipartimento di Scienze della Terra e dell'Ambiente, Pavia, Italy D' Ambrosio S., Center for Biostatistics & Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA Dabrowska M., Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland Dalan A.B., Yeditepe University Hospital, Istanbul, Turkey Dalianis T., Department of Oncology, Karolinska Institutet, Stockholm, Sweden Damaskos C., Second Dept. of Propedeutic Surgery, “Laiko” General Hospital, National and Kapodistrian Univ. of Athens, Medical School, Athens, Greece Danileviciute V., Faculty of Medicine, Vilnius University, Vilnius, Lithuania Dasu A., Dept. of Radiation Physics and Department of Medical and Health Sciences, Linköping University, Linköping, Sweden Dauksa A., Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania Davidson B., Saint James School of Medicine, Anguilla, British West Indies De Bari B., Radiation Oncology Department, Centre Hospitalier Universitaire Vadois, Lausanne, Switzerland De Brabander J.K., Dept of Biochemistry and Harold C. Simmons Comprehensive Cancer Center, The Univ. of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA De Braekeleer Marc, Faculté de Médecine et des Sciences de la Santé & CHRU Brest, Brest, France De Grève J., Familial Cancer Clinic and Medical Oncology, Oncologisch Centrum UZ Brussel, Brussels, Belgium De Marchis M.L., Dept. of Cardiovascular, Respiratory, Nephrologic, Geriatric and Anesthesiological Sciences, Sapienza University of Rome, Policlinico Umberto I, Rome, Italy De Meirleir K., Himmunitas Foundation, Neder-Over-Heembeek, Belgium De Oliveira J.T., Institute of Molecular Pathology and Immunology (IPATIMUP), University of Porto, Porto, Portugal De Vogelaere K., Department of Abdominal Surgery, UZ Brussel, Brussels, Belgium De Witty Jr. R.L., Howard University Hospital, Washington, DC, USA Delebinski C.I., Department of Pediatric Oncology/ Hematology, Otto-Heubner-Centre for Pediatric and Adolescent Medicine (OHC), Charité, Universitätsmedizin, Berlin, Germany Deliu Z., Dept. of Oral Medicine and Diagnostic Sciences, College of Dentistry, Univ. of Illinois at Chicago, Chicago, IL, USA Deppert W., Institute for Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany Desnoyers G., Atlantic Cancer Research Institute, Moncton, NB, Canada Dettling S., Department of Neurosurgery, Division of Neurosurgical Research, University Hospital, Heidelberg, Germany Dilruba S., Institute of Pharmacy, Clinical Pharmacy, University of Bonn, Bonn, Germany Dima S.O., Fundeni Clinical Institute, Bucharest, Romania Dimitroulakos J., Centre for Cancer Therapeutics at the Ottawa Hospital Research Institute, Ottawa, ON, Canada Dimov D.M., Department of Internal Medicine, Medical Faculty, Trakia University, Stara Zagora, Bulgaria Diorio C., Oncology Unit, CHU de Québec Research Center, Saint-Sacrement Hospital, Quebec City, QC, Canada Dobrota D., Department of Medical Biochemistry, Comenius Univ. in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic Douik H., Service de Biologie Clinique, Institut Salah Azaiz de Tunis, Tunis, Tunisie Dovas A., Department of Pathology, Columbia University Medical Center, New York, NY, USA Drozdowska D., Department of Organic Chemistry, Medical University, Białystok, Poland Duburs G., Latvian Institute of Organic Synthesis, Riga, Latvia Dudek A., Department of Medicine, Cancer Center, University of Illinois at Chicago, Chicago, IL, USA Dufès C., Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK Dumitrascu T., Center of General Surgery and Liver Transplant, Fundeni Clinical Institute, Bucharest, Romania Dunn J., Cancer Council Queensland, Fortitude Valley, Brisbane, QLD, Australia Dus-Szachniewicz K., Department of Pathology, Wrocław Medical University, Wroclaw, Poland Dziegiel P., Department of Histology/Embryology, Wrocław Medical University, Wroclaw, Poland Eccleston M., VolitionRX, Namur, Belgium Efferth T., Department of Pharmaceutical Biology, Johannes Gutenberg University Mainz, Mainz, Germany El Habbasha S.F., Medicinal and Aromatic Plants Department, Pharmaceutical and Drug Industries Division, National Research 5770

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Centre, Cairo, Egypt El Sharouni S., University Medical Center, Utrecht, The Netherlands Engström W., Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine, SLU, Uppsala, Sweden Ensinger C., Medical University of Innsbruck, Innsbruck, Austria Errico C., Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore Sanità, Rome, Italy Eskelinen M., Department of Surgery, Kuopio University Hospital, Kuopio, Finland Etrych T., Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic Evangelisti C., Cell Signaling Laboratory, Dept. of Biomedical and Neuromotor Sciences (DIBINEM), Univ. of Bologna, Bologna, Italy Fabbri F., Bioscience Laboratory, Romagna Cancer Institute – IRCCS IRST, Meldola, Italy Facoetti A., Fondazione CNAO, Pavia, Italy Farinha Garcao Nunes J., Imperial College London, Hammersmith Campus, Department of Surgery and Cancer, London, UK Farkouh A., Division of Clinical Pharmacy and Diagnostics, Faculty of Life Sciences, University of Vienna, Vienna, Austria Faustino-Rocha A., Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Felsher D.W., Department of Medicine-Oncology and Pathology, Stanford University, Stanford, CA, USA Fenical W., University of California at San Diego, La Jolla, CA, USA Ferda J., Clinic of The Imaging Methods, University Hospital, Pilsen, Czech Republic Ferreira M-J.U., Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Univ. of Lisbon, Lisbon, Portugal Ferreira-Parker J., Universidad De Chile, Independencia, Santiago, Chile Ferroni P., Telematic University Ran Raffaele, Biomarker Discovery and Advanced Biotechnology (BioDAT) Laboratory, IRCCS San Raffaele Pisana – Research Center, Rome, Italy Fiala O., Departments of Oncology and Radiotherapy, Medical Sch. and Teaching Hospital in Pilsen, Charles Univ. in Prague, Prague, Czech Republic Fici P., Biosciences Laboratory, IRCCS Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST), Meldola, Italy Fields A.P., Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, FL, USA Fillmore H., Cellular and Molecular Neuro-oncology Research Group, University of Portsmouth, UK Fkih M'hamed I., Département d’Oncogénétique, Centre Jean Perrin, Clermont-Ferrand, France Flejmer A.M., Department of Oncology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden Fountzilas G., Department of Medical Oncology, Papageorgiou Hospital, Aristotle University of Thessaloniki School of Medicine, Thessaloniki, Greece Fourkala R., Women's Cancer Department, Institute for Women's Health, University College London (UCL), UK Frikha A., Department of Medical Oncology, Polyclinique Maymard, Bastia, France Frosini M., Department of Life Sciences, University of Siena, Siena, Italy Fuchs D., Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Innsbruck, Austria Fuchsova R., Immunoanalytical Laboratory, Department of Nuclear Medicine, University Hospital in Pilsen, Pilsen, Czech Republic Fuentes-Retamal S., Clinical and Molecular Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile Fukagawa S., Department of Obstetrics and Gynecology, Fukuoka University, Fukuoka, Japan Funel N., Division of General and Transplantation Surgery, University of Pisa and Pisa University Hospital, Pisa, Italy Gabius H.-J., Institute of Physiological Chemistry, Ludwig-Maximilians-University Munich, München, Germany Gaje P.N., Dept. of Microscopic Morphology/Histology, Angiogenesis Research Center, “Victor Babes” Univ. of Medicine and Pharmacy, Timisoara, Romania Galindo Hernández O., Departamento de Biología Celular, CINVESTAV-IPN, México D.F., México Ganapathi M.K., Levine Cancer Institute, Carolinas HealthCare System, Charlotte, NC, USA Ganapathi R., Levine Cancer Institute, Carolinas HealthCare System, Charlotte, NC, USA Ganatra S.H., Department of Chemistry, Institute of Science, Nagpur, Maharashtra, India 5771

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Gandhi V.V., Center for Targeted Therapy, MD Anderson Cancer Center, Unit 1950, Houston, TX, USA Gao F., Department of Molecular Biology Laboratory, Shanghai Sixth People’s Hospital, Shanghai Jiaotong Univ. School of Medicine, Shanghai, PR China Gärtner M.F., Institute of Molecular Pathology and Immunology (IPATIMUP), University of Porto, Porto, Portugal Gazzano E., Department of Oncology, University of Torino, Torino, Italy Georgieva E., Department of Medicinal Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria Georgopoulos A., Department of Internal Medicine I, General Hospital Vienna, Vienna, Austria Gerwick W.C., Scripps Institution of Oceanography and Skaggs Sch. of Pharmacy and Pharmac. Sciences, Univ. of California, San Diego, CA, USA Getoff N., The University of Vienna, Section of Radiation Biology, Department of Nutritional Sciences, Vienna, Austria Gezer U., Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey Ghotra P.K., College of Physiotherapy, Christian Medical College and Hospital, Ludhiana, Punjab, India Giamas G., Imperial College London, Hammersmith Campus, Department of Surgery and Cancer, London, UK Gil J., Genetics Department, Wroclaw Medical University, Wroclaw, Poland Gil-Bernabe A.-M., CRUK & MRC Oxford Institute for Radiation Oncology, Department of Oncology, Univ. of Oxford, Oxford, UK Giorgio L., Cancer Biology Group, The Basil Hetzel Inst. for Transl. Health Research, Sch. of Medicine, Univ. of Adelaide, Adelaide, SA, Australia Glavan D., Institute of Oncology, Bucharest, Romania Gloushankova N., N.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences, Moscow, Russia Gocze K., Institute of Public Health, University of Pécs, Pécs, Hungary Goffart N., Laboratory of Developmental Neurobiology, GIGA-Neurosciences Research Center, University of Liège, Liège, Belgium Gombos K., Department of Public Health, University of Pécs, Pécs, Hungary Gonzalez-Hormazabal P., Human Genetics Program, Institute of Biomedical Sciences, University of Chile, Santiago, Chile Goossens M., Brussels, Belgium Gorchs L., University of Tromsø, Tromsø, Norway Görmüş U., Bilim University, Faculty of Medicine, Department of Clinical Biochemistry, İstanbul, Turkey Gospodinova Z., Department of Molecular Genetics, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria Gostner J.M., Division of Medical Biochemistry, Biocenter, Innsbruck Medical University, Innsbruck, Austria Goswami P.C., Free Radical and Radiation Biology Division, Dept. of Radiation Oncology, The University of Iowa, Iowa City, IA, USA Gounaris A., Research Center, Hellenic Anticancer Institute, Athens, Greece Goussia A.C., Department of Pathology, Medical School, University of Ioannina, Ioannina, Greece Greniuk-Maciejewska T., Medical University of Lublin, Lublin, Poland Grisendi G., Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, Modena, Italy Gruia M.I., Institute of Oncology, Bucharest, Romania Guadagni F., Telematic University San Raffaele, IRCCS San Raffaele Pisana, Rome, Italy Guerra B., Biomedical Research Group, BMB, University of Southern Denmark, Odense, Denmark Gulubova M., Department of General and Clinical Pathology, Medical Faculty, Trakia University, Stara Zagora, Bulgaria Gupta K., Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, Lucknow, India Guttenplan J.B., New York University Dental College, New York, NY, USA Guzinska-Ustymowicz K., Department of General Pathomorphology, Medical University of Bialystok, Bialystok, Poland Hafizi S., Institute of Biomedical and Biomolecular Science, University of Portsmouth, Portsmouth, UK Halasova E., Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Department of Medical Biology, Martin, Slovakia Hamann M.T., Department of Pharmacognosy, Pharmacology, Chemistry and Biochemistry & The National Center for Natural Products Research, UMMC Cancer Center, University of Mississippi, University, MS, USA Harvey A.J., Brunel Institute for Cancer Genetics and Pharmacogenomics, Biosciences, Brunel University (London), Uxbridge, Middlesex, UK 5772

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Haybaeck J., Institute of Pathology, Medical University of Graz, Graz, Austria Hazra T.K., Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine and Sealy Center for Molecular Medicine, University of Texas Medical Branch, Galveston, TX, USA Helke K., Department of Comparative Medicine, Medical University of South Carolina, Charleston, SC, USA Hellevik T., Radiation Therapy Department, University-Hospital Northen Norway, Tromsø, Norway Hellstrand K., Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden Helson L., SignPath Pharma, Inc., Quakertown, PA, USA Hensler S., Faculty of Medical and Life Sciences, Furtwangen University, Villingen-Schwenningen, Germany Herold-Mende C., Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany Herzog M., Belgian Volition SA, Gossieles, Belgium Hilakivi-Clarke L., Department of Oncology, Georgetown University, Washington, DC, USA Hoeschele J.D., Department of Chemistry, Eastern Michigan University, Ypsilanti, MI, USA Hoffman R.M., AntiCancer Inc., San Diego, CA, USA Hofmanova J., Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Holdenrieder S., Institut für Klinische Chemie und Klinische Pharmakologie-Zentrallabor-Universitätsklinikum Bonn, Bonn, Germany Holst J., Centenary Institute and Sydney Medical School, University of Sydney, Camperdown, NSW Australia Hong T.-M., Graduate Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan Horwitz S., Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA Hsu C.-M., Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC Huang C.-Y., Taichung Armed Forces General Hospital, Taichung, Taiwan, ROC Huang K.-B., Guangxi Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy of Guangxi Normal University, Guilin, PR China Hunter K.D, Clinical Senior Lecturer in Oral Pathology, Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, University of Sheffield, Sheffield, UK Hunyadi A., Institute of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary Iacopino F., Istituto di Istologia ed Embriologia, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Rome, Italy Ibrahim B., Urology Department, Saber Hospital, Aden, Yemen Ibrahim F.M., Medicinal and Aromatic Plants Department, Pharmaceutical and Drug Industries Division, National Research Centre, Cairo, Egypt Iglesias-Gato D., NNF-Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark Ikitimur-Armutak E.I., Istanbul University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Istanbul, Turkey Ilhan N., Fırat University, Medical Faculty, Department of Medical Biochemistry, Elazig, Turkey Illemann M., The Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark Ilyas M., Academic Unit of Pathology, Division of Cancer and Stem Cell Science, University of Nottingham, Queen’s Medical Centre Nottingham, UK İnal-Gultekin G., Department of Mol. Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey Inan S., Celal Bayar University, Faculty of Medicine, Dept. of Histology & Embryology, Manisa, Turkey Ingvarsson S., Institute for Experimental Pathology and Faculty of Medicine, University of Iceland, Keldur, Iceland Isbir T., Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey Ishikawa M., Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan Ivanova K., Department of General and Clinical Pathology, Medical Faculty, Trakia University, Stara Zagora, Bulgaria Jackson J., University of British Columbia, Vancouver, BC, Canada Janeklang S., Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Janin A., Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Pathologie, UMR-S 1165, Paris, France Januchowski R., Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland 5773

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Jassam S., Cellular and Molecular Neuro-oncology Group, University of Portsmouth, Portsmouth, UK Jave-Suárez L.F., División de Inmunología, Centro de Investigación Biomédica de Occidente, IMSS, Guadalajara, Jalisco, Mexico Jean-Claude B.J., Cancer Drug Research Laboratory, Department of Medicine, Division of Medical Oncology, McGill University Health Center/Royal Victoria Hospital, Montreal, QC, Canada Jernström H., Division of Oncology and Pathology, Department of Clinical Sciences, Lund University Cancer Center/Kamprad, Lund, Sweden Jha G.J., Division of Hematology and Oncology, University of Minnesota, Minneapolis, MN, USA Jhanwar S., Memorial Sloan Kettering Cancer Center, Laboratory of Solid Tumor Genetics, New York, NY, USA Jhanwar-Uniyal M., Department of Neurosurgery, New York Medical College, Valhalla, NY, USA Jiang W.G., Cardiff University School of Medicine, Cardiff, UK Johnson C.E., Institute of Cancer and Genetics, Cardiff University, Cardiff, UK Judson I., Sarcoma Unit, Royal Marsden Hospital, London, UK Juniku-Shkololli A., University Clinical Center of Kosovo, Department of Pathophysiology, Prishtina, Republic of Kosovo Juršėnaitė J., Center for Innovative Medicine, Vilnius, Lithuania Kaetzel D.M., Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA Kaina B., Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Mainz, Germany Kalamida D., Department of Radiotherapy – Oncology and Radiobiology-Radiopathology Unit, Medical School, Democritus University of Thrace, Alexandroupolis, Greece Kallay E., Department of Pathophysiology and Allergy Research, Medical University of Vienna, Austria Kallianos A., 2nd Pulmonary Clinic Sotiria Hospital Athens, Athens, Greece Kalogera E., Research Center, Hellenic Anticancer Institute, Athens, Greece Kamba S.A., Laboratory of Molecular Biomedicine, Institute of Bioscience, University Putra Malaysia, Selangor Darul Ehsan, Malaysia Kanaan Y.M., Department of Microbiology, Cancer Center, College of Medicine, Howard University, Washington, DC, USA Kaneko H., Department of Surgery, School of Medicine, Toho University, Tokyo, Japan Kara G.J., Chemistry Department, Biochemistry Division, Hacettepe University, Beytepe, Ankara, Turkey Karagounis I.V., Radiobiology and Radiopathology Unit, Department of Radiotherapy/Oncology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece Karanikas G., Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, Vienna, Austria Karlsson U., Marshfield Clinic, Marshfield, WI, USA Karteris E., Cellular Endocrinology Laboratory, Department of Biosciences, Brunel University, London, UK Karydas I., Breast Surgeon, Director of Breast Center "HYGEIA" Hospital, Athens, Greece Kastler B., University of Franche-Comté, Radiologie A & C, University Hospital and Director of Laboratoire I4S, CHU Minjoz, Besançon, France Katanaev V., Dept. of Pharmacology and Toxicology, Faculty of Biology and Medicine, Univ. of Lausanne, Lausanne, Switzerland Kawasaki S., Department of Hepatobiliary Pancreatic Surgery, Juntendo University, Tokyo, Japan Kayser K., Institute of Pathology, Charite, Berlin, Germany Keatley K., Cellular and Molecular Neuro-oncology Research Group, University of Portsmouth, UK Keisari Y.M., Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, Israel Kessous D., Ben Yair Community Clinic, Beer-Sheva, Israel Khalifa M., Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada Khotimchenko M., School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia Khotimchenko Y., School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia Kieback D.G., Klinikum Vest, Standort Marl, Marl, Germany Kinkorova΄ J., Department of Plastic Surgery, University Hospital and Charles University in Plzen, Plzen, Czech Republic Kirti, Department of Urology, King George's Medical University, Lucknow, Uttar Pradesh, India 5774

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Kiss I., Institute of Preventive Medicine, University of Pécs Medical School, Pécs, Hungary Kiss Z., CanCure Laboratories LLC, Austin, MN, USA Klapdor R., Internal Unit, ZeTDT GmbH, Hamburg, Germany Klein J., University Hospital Olomouc, Olomouc, Czech Republic Kleinsimon S., Department of Pediatric Oncology/ Hematology, Otto-Heubner-Centre for Pediatric and Adolescent Medicine (OHC), Charité, Universitätsmedizin, Berlin, Germany Klump H., Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany Knutson K.L., Vaccine & Gene Therapy Institute of Florida, Port Saint Lucie, FL, USA Kobierzycki C., Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Koch J., University of Copenhagen, Division of Health Sciences, Department of Experimental Medicine, Copenhagen, Denmark Köberle B., Karlsruhe Institute of Technology, Food Chemistry and Toxicology, Karlsruhe, Germany Kohonen-Corish M., Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, NSW, Australia Konarikova K., Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic Konrad M., Enzyme Biochemistry Laboratory, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany Koontongkaew S., Faculty of Dentistry, Thammasat University, Pathum Thani, Thailand Kopitz J., Department of Applied Tumor Biology, Ruprecht-Karls-University Heidelberg, Heidelberg, Germany Korhonen R., Department of Surgery, School of Medicine, University of Eastern Finland, Kuopio Campus, Kuopio, Finland Kottaridis S.D., Hellenic Anticancer Institute, Athens, Greece Koukourakis M.I., Department of Radiotherapy-Oncology, Democritus University of Thrace Medical School, Alexandroupolis, Greece Koutsilieris M., Laboratory of Experimental Physiology, Department of Basic Sciences, University of Athens Medical School, Athens, Greece Kowalewska M., Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland Kraemer S., Breast Center, Department of Gynaecology and Obstetrics, University-Hospital of Cologne, Cologne, Germany Książkiewicz M., Celther Polska Sp. z o. o., Łódź, Poland Kuang B.-H., State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, PR China Kubista M., TATAA Biocenter, Göteborg, Sweden Kubota K., Second Department of Surgery, Dokkyo Medical University, Tochigi, Japan Kucera R., Laboratory of Immunoanalysis, Faculty Hospital in Pilsen, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic Kuchmenko O.B., National Scientific Centre “Strazhesko Institute of Cardiology” of NAMS of Ukraine, Kyiv, Ukraine Kujovská Krčmová L., Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic Kumar P., Division of Laboratory & Regenerative Medicine, University of Manchester Medical School, Manchester, UK Kumar S., Department of Biological Sciences, Manchester Metropolitan University, Manchester, UK Kust D., University Hospital Center ''Sestre Milosrdnice'', Zagreb, Croatia Laczmanska I., Genetics Department, Wroclaw Medical University, Wroclaw, Poland Laerum O.D., Department of Pathology & Oncology, Institute of Clinical Research, Haukeland University Hospital, Bergen, Norway Lage H., Charité Campus Mitte, Institute of Pathology, Berlin, Germany Lamprecht S., Division of Clinical Biochemistry and Pharmacology, Ben-Gurion University of the Negev, Beer-Sheva, Israel Lamprou D., Strathclyde Institute of Pharmacy & Biomedical Sciences (SIPBS) University of Strathclyde, Glasgow, Scotland, UK Lapini A., Department of Urology, University of Florence, Florence, Italy Larsen R.H., Oncoinvent AS, Oslo, Norway Lascialfari A., Department of Physics and INSTM Unit, Università degli Studi di Milano, Milan, Italy Laube F., Institute of Physiological Chemistry, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany Laurinavičius S., Laboratory of Molecular Oncology, National Institute of Cancer, Vilnius, Lithuania Lazarou A., Department of Obstetrics, Charité, Virchow Campus Clinic University-Hospital, Berlin, Germany 5775

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Leitges M., Biotechnology Centre of Oslo, Oslo, Norway Lejeune F.J., Faculty of Biology and Medicine, Lausanne University, Lausanne, Switzerland Leman E., Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel Levy J., Department of Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel Li R., Center for Drug Discovery and Department of Pharmaceutical Sciences, Coll. of Pharmacy Cancer Genes and Mol. Regulation Program, Fred and Pamela Buffett Cancer Center University of Nebraska Medical Center, Omaha, NE, USA Lichtenegger W.G., Charité Campus Virchow-Klinikum, Klinik für Frauenheilkunde und Geburtshilfe, Berlin, Germany Lichty B.D., McMaster Immunology Research Centre, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada Lincoln D., Independent Research Foundation, Chapel Hill, QLD, Australia Lintzeris I., Surgery Oncology Department and Microbiology Laboratory of Anticancer Hospital of Piraeus “ Metaxa”, Piraeus, Greece Liotsou T., 2nd Department of Radiology, Nuclear Medicine Section, National and Kapodistrian University of Athens, Medical School, Athens, Greece Liska V., Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic Lisowska K.M., Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland Lissoni P., Italian Association of Integrate Medicine, Milan, Italy Littlefield B.A., Eisai Inc., Andover, MA, USA Liu Y.-C., State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry & Pharmaceutical Sciences, Guangxi Normal University, Guilin, PR China Liu Y., Department of Molecular Biology Laboratory, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China Livney Y.D., Laboratory of Biopolymers and Food Nanotechnology, Faculty of Biotechnology & Food Engineering, and The Russell Berrie Nanotechnology Institute, The Technion, Israel Institute of Technology, Haifa, Israel Luecke K., GILUPI GmbH, Potsdam, Germany Łukawska M., Department of Modified Antibiotics, Institute of Biotechnology and Antibiotics, Warsaw, Poland Lumachi F., University of Padua, School of Medicine, Padova, Italy Lunde M.S., Department of Teacher Education and School Research, University of Oslo, Oslo, Norway Luqman S., Academy of Scientific and Innovative Research, Molecular Bioprospection Department of Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India Mabeta P., Angiogenesis Laboratory, Department of Anatomy and Physiology, University of Pretoria, Tshwane, South Africa Maciejewski R., Laboratory of Biostructure, Human Anatomy Department, Medical University of Lublin, Lublin, Poland Maeda H., Institute of Drug Delivery Science, Sojo University, Kumamoto, Japan Maehara Y., Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Mahfoud W., Biology and Health Laboratory, Faculty of Sciences Ben M’sik, Hassan II-University Mohammedia, Casablanca, Morocco Man Y.-G., Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, USA Mandal C.C., Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Rajasthan, India Mangiola A., Institute of Neurosurgery, Faculty of Medicine, Catholic University of Sacred Heart, Rome, Italy Manome Y., Division of Molecular Cell Biology, Core Research Facilities for Basic Science, Jikei University School of Medicine, Tokyo, Japan Mantso T., School of Life Sciences, Heriot Watt University, Edinburgh, UK Marchoudi N., Laboratoire de Physiopathologie et Génétique Moléculaire, Faculté des Sciences Ben M’sik-Casablanca, Université Hassan II-Mohammedia, Casablanca, Morocco Martignoni G., Deparment of Pathology, University of Verona, Verona, Italy Martin S.S., Marlene and Stewart Greenebaum NCI Cancer Center, University of Maryland School of Medicine, Baltimore, MD, USA Martinez-Zubiaurre I., Department of Clinical Medicine, University of Tromsø, Tromsø, Norway Martins A., Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary 5776

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Martner A., Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden Martorana G., Deprtment of Urology, University of Bologna, Bologna, Italy Matakova T., Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Department of Medical Biochemistry, Martin, Slovakia Mather R., Cellular and Molecular Neuro-oncology Group, University of Portsmouth, Portsmouth, UK Matsumoto Y., Department of Life Sciences, Sojo University, Kumamoto, Japan Matuschek C., Heinrich Heine University, Düsseldorf, Germany Mavroidi B., Inorganic Chemistry Laboratory, Department of Chemistry, University of Athens, Athens, Greece Mazur L., Department of Experimental Hematology, Jagiellonian University in Krakow, Kraków, Poland Mazure N.M., Institute of Developmental Biology and Cancer Research, University of Nice, Nice, France McHale A., School of Pharmacy and Pharmaceutical Sciences, University of Ulster, Coleraine, Northern Ireland Mehta K., Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA Mellai M., Neuro-bio-oncology Center, Policlinico di Monza Foundation, Vercelli, Italy Messina G., Italian Association of Integrated Medicine, Milan, Italy Methenitis C., Inorganic Chemistry Laboratory, Department of Chemistry, University of Athens, Athens, Greece Micallef J., Belgian Volition SA, Gossieles, Belgium Michael A., Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK Michelakis D., Department of Surgical Oncology, University Hospital of Crete, Heraklion, Greece Milczarek M., Department of Cell Biology, Confocal Microscopy Laboratory, National Medicines Institute, Warsaw, Poland Mirossay L., Department of Pharmacology, Pavol Jozef Šafárik University, Faculty of Medicine, Kosice, Slovak Republic Mishra R., Institute of Animal Physiology and Genetics, Libechov, Czech Republic Mitra S., Department of Radiation Oncology, Houston Methodist Research Institute, Galveston, TX, USA Mitrakas A.G., Radiobiology and Radiopathology Unit, Department of Radiotherapy/Oncology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece Miyata K., Deptertment of Obstetrics and Gynecology, School of Medicine, Fukuoka University, Fukuoka, Japan Mohd Mutalip S.S., Faculty of Medicine, Universiti Teknologi MARA (UiTM) Sg. Buloh, Selangor, Malaysia Mojzis J., Department of Pharmacology, Pavol Jozef Šafárik University, Faculty of Medicine, Kosice, Slovak Republic Mojzisová G., Department of Experimental Medicine, Faculty of Medicine, P.J. Safarik University, Kosice, Slovak Republic Mol J.A., Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands Molina R., Laboratory of Biochemistry (Oncobiology Unit), Thorax Institute Hospital Clínic, IDIBAPS, Barcelona, Spain Molnár J., Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary Mooberry S.L., Graduate School of Biomedical Sciences, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA Moore M., Chief Editor of the Asian Pacific Journal of Cancer Prevention, National Cancer Center of Korea, Gyeonggi-do, Republic of Korea Morgan R., Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK Morin R., Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B.C., Canada Motaln H., Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany Mourad W.F., Department of Radiation Oncology, Beth Israel Medical Center, New York, NY, USA Mueller M.M., Department Biomedical Engineering, Faculty of Medical and Life Sciences, Hochschule Furtwangen University, Villingen-Schwenningen, Germany Muftuoglu S., Department of Histology & Embryology, Hacettepe University Faculty of Medicine, Ankara, Turkey Muggia F., New York University School of Medicine and NYU Cancer Institute, New York, NY, USA Murakami N., Department of Radiation Oncology, National Cancer Center Hospital, Tokyo, Japan Murray N.R., Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, FL, USA Nagler R., Department of Oral and Maxillofacial Surgery and Oral Biochemistry Laboratory, Rambam Medical Center and Rappaport Faculty of Medicine, Israel Institute of Technology-Technion, Haifa, Israel Naimi B.Y., AnyGenes, Hôpital Tenon, Paris, France 5777

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Nakamura K., Deparmtent of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University, Nishinomiya, Hyogo, Japan Nanni U., Department of Computer, Control and Management Engineering, Sapienza University, Rome, Italy Nano R., Dipartimento Biologia e Biotecnologie "Lazzaro Spallanzani", Università degli Studi di Pavia, Pavia, Italy Naor D., Department of Immunology, The Lautenberg Center for Immunology and Cancer Research, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel Narayanan R., Department of Biological Sciences, Charles E. Schmidt College of Science, Florida Atlantic University, Boca Raton, FL, USA Naz A., Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan Nguyen A.V., Department of Biological Sciences and Geology, Queensborough-CUNY, Bayside, NY, USA Nguyen N., Department of Radiation Oncology, Howard University, Washington, DC, USA Nicolini C., Laboratories of Biophysics and Nanobiotechnology, Department Experimental Medicine, University of Genova, Genova, Italy Niewiarowska K., Department of General Pathomorphology, Medical University of Bialystok, Bialystok, Poland Nishikata T., Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan Nonni A., 1st Department of Pathology, Medical School, University of Athens, Athens, Greece Nowacki L., Université de Technologie de Compiègne, CNRS FRE 3580, Compiègne, France Nutthasirikul N., Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand Nysocka T., Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Ohno T., Nagasaki Prefecture Shimabara Hospital, Nagasaki, Japan Okamoto T., Dept. of Surgery and Science, Graduate School of Medicine, Kyushu University, Fukuoka, Kyushu, Japan Oklah N., Urology Department, Saber Hospital, Aden, Yemen Olbryt M., Center for Transl. Research and Mol. Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Inst. of Oncology, Gliwice Branch, Gliwice, Poland Onal E., Department of General Medical Faculty, Trakia University, Stara Zagora, Bulgaria Onal T., Celal Bayar University, Faculty of Medicine, Department of Histology and Embryology, Manisa, Turkey Önder Mert N.İ., Department of Biology, Anadolu University, Life Sciences Institute, Eskişehir, Turkey Ontario M.L., Health and Scientific Division, Research and Development Division, Ontario Srl, Catania, Italy Ontario S., Young Entrepreneurs of Confindustria, Ontario Srl, Catania, Italy Ooi H., Department of Med. Imaging and Radiological Sciences, Central Taiwan Univ. of Sci. and Technology, Taiwan, ROC Orsós Z., Department of Public Health, Medical School, University of Pécs, Pécs, Hungary Osada S., Surgical Oncology, Gifu University School of Medicine, Gifu, Japan Ozay Y., Department of Medical Biology, Faculty of Medicine, Adıyaman University, Adıyaman, Turkey Ozgul M., Department of Histology and Embryology, Celal Bayar University, Faculty of Medicine, Manisa, Turkey Ozturk M., Istanbul University, Cerrahpasa Medicine Faculty, Medical Biology Department, Istanbul, Turkey Pajkos G., Bacs-Kiskun County Teaching Hospital Center of Oncoradiology, Kecskemét, Hungary Palakurthi S., Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Kingsville, TX, USA Palmirotta R., IRCCS San Raffaele Pisana - Telematic University San Raffaele Roma, Rome, Italy Panagiotidis M.I., School of Life Sciences, Heriot Watt University, Edinburgh, UK Pandita R.K., Department of Radiation Oncology, The Houston Methodist Research Institute, Houston, TX, USA Pandita T.K., Department of Radiation Oncology, The Houston Methodist Research Institute, Houston, TX, USA Pappagallo G.L., Department of Medical Oncology, Mirano, Italy Papagerakis P., Department of Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, MI, USA Papagerakis S., Dept. of Otolaryngology - Head & Neck Surgery, Medical School, Univ. of Michigan, Ann Arbor, MI, USA Papagrigoriadis S., Department of Colorectal Surgery, King’s College Hospital, London, UK Paschos K.A., Liver Research Group, Dept. of Oncology, School of Medicine, Royal Hallamshire Hospital, The Univ. of Sheffield, Sheffield, UK 5778

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Pastwa E., Laboratory of Molecular Genetics, Medical University of Lodz, Lodz, Poland Patel M.R., Dept. of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA Pathak S., Department of Genetics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA Patterson G., University of Texas Health Science Center at San Antonio, San Antonio, TX, USA Pechkova E., Laboratories of Biophysics and Nanobiotechnology, Department Experimental Medicine, University of Genova, Genova, Italy Pehlivan M., Department of Biology, Faculty of Science and Art, University of Gaziantep, Gaziantep, Turkey Penichet M., Division of Surgical Oncology, Department of Surgery, University of California, Los Angeles, CA, USA Pentimalli F., Oncology Research Center of Mercogliano (CROM), Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione Giovanni Pascale”; IRCCS, Naples, Italy Peppa V., Laboratory of Physiology, Dept. of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece Peredo-Silva L., Clinical and Molecular Pharmacology Program, ICBM, Faculty of Medicine and Univ. of Chile, Santiago, Chile Perjési P., Institute of Pharmaceutical Chemistry, University of Pécs, Pécs, Hungary Pfankuchen D., Pharmaceutical Department, University of Bonn, Bonn, Germany Phumyen A., Biomedical Sciences Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand Pilkington G., Cellular and Molecular Neuro-oncology Research Group, University of Portsmouth, Portsmouth, UK Pinchuk I.N., Surgut District Hospital, Surgut, Russia Piukovics K., University of Szeged, Second Department of Medicine and Cardiology Centre, Szeged, Hungary Plaimee P., Melatonin Research Group, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand Pochi S.R., Hematology and Oncology, Univ. of Miami Miller, School of Medicine, Miami, FL, USA. Podhorska-Okolow M., Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Polliack A., Department of Hematology, Hadassah University Hospital, Jerusalem, Israel Pongracz J., Department of Pharmaceutical Biotechnology, The Medical School, University of Pécs, Pécs, Hungary Poplawski T., Department of Molecular Genetics, University of Lodz, Lodz, Poland Porta C., Medical Oncology, I.R.C.C.S. San Matteo University Hospital Foundation, Pavia, Italy Porta E., AIMI Association, Milan, Italy Postema M., Oncology PCU (US), EISAI Andover Site, Andover, MA, USA Pottier A., Nanobiotix, Paris, France Pouliliou S., Radiobiology and Radiopathology Unit, Dept of Radiotherapy/Oncology, Sch. of Medicine, Democritus Univ. of Thrace, Alexandroupolis, Greece Presl J., Department of Gynecology and Obstetrics, Faculty Hospital in Pilsen, Pilsen, Czech Republic Priel E., The Shraga Segal Dept. of Microbiology, Immunology & Genetics, Faculty of Health Sciences, Ben-Gurion Univ. of the Negev, Beer-Sheva, Israel Prinz C., Lehrstuhl für Innere Medizin 1, Universität Witten/Herdecke, Witten, Germany Provatopoulou X., Research Center, Hellenic Anticancer Institute, Athens, Greece Provenzano L., Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth, Devon, UK Pryczynicz A., Department of General Pathomorphology, Medical University of Bialystok, Bialystok, Poland Qiao Y.-L., Sciences and Peking Union Medical College, Beijing, PR China Queiroga F.L., University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Radosevich J.A., Center for Mol. Biology of Oral Diseases, Dept. of Oral Medicine and Diagnostic Sciences, Univ. of Illinois at Chicago, Chicago, IL, USA Raica M., Victor Babes Univ. of Medicine and Pharmacy, Dept. of Morphologic Microscopy, Angiogenesis Res. Center, Timisoara, Romania Rakosy Z., Department of Public Health Medicine, University of Pécs, Pécs, Hungary Ramachandran G.K., National University of Singapore, Singapore Ramonaite R, Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania Ramos L.C.B., Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Monte Alegre, Ribeirão Preto, SP, Brazil Ramqvist T., Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden 5779

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Rapp C., Univ. of Heidelberg, Department of Neurosurgery, Division of Experimental Neurosurgery, Heidelberg, Germany Rapti A., 2nd Pulmonary Dept., Sotiria Hospital, Athens, Greece Rashid S., Section of Mol. Carcinogenesis and Chemoprevention, Dept. of Med. Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard Univ.), Hamdard Nagar, New Delhi, India Ray A., Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, USA Ray B.K., Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, USA Recchia F., Civilian Hospital, Avezzano, Italy Reinwald K., "Dr. Reinwald Healthcare GMBH + CO KG", Schwarzenbruck, Germany Restucci B., Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy Reyes-Uribe E., Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Dept. de Biología Celular, D.F., México Reyland M.E., Dept. of Craniofacial Biology, Univ. of Colorado Anschutz Medical Campus, Aurora, CO, USA Reynaud C., INSERM, UMR1033, Lyon, France Riboni L., Department of Medical Biotechnology and Translational Medicine, LITA-Segrate, Univ. of Milan, Milan, Italy Richman S., Dept. of Pathology and Tumour Biology, Leeds Inst. of Cancer and Pathology, St James Hospital, Leeds, UK Rigaud M., ASTRALAB, Clinical Laboratory, Limoges, France Riondino S., Biomarker Discovery and Advanced Technologies (BioDAT) Laboratory, IRCCS San Raffaele Pisana, Research Center, Rome, Italy Robertson F.M., Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA Rochel N., IGBMC, Illkirch, France Rode W., Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland Rodriguez Varas A., Universidad De Chile, Independencia, Santiago, Chile Roelant C., Protea Biopharma N.V., Neder-Over-Heembeek, Belgium Romo D., Dept. of Chemistry and The Nat. Products LINCHPIN Laboratory, Texas A&M Univ., College Station, TX, USA Roos A., Veterinary Referral Centre Gouda, Gouda, The Netherlands Roselli M., Dept. of Systems Medicine, Tor Vergata Clinical Center, Univ. of Rome “Tor Vergata”, Rome, Italy Rosi G., Dept. of Surgical and Biom. Sciences, Division of Radiology 2, Perugia Univ., S. Maria della Misericordia Hospital, Perugia, Italy Rossi C., Université de Technologie de Compiègne, Compiègne, France Rossignoli F., University of Modena and Reggio Emilia, Verona, Italy Rotar I.C., Mother and Fetus Department, “Iuliu Haţieganu” Univ. of Medicine and Pharmacy Cluj Napoca, Cluj Napoca, Romania Roupa I., Inorganic Chemistry Laboratory, Dep. of Chemistry, Univ. of Athens, Athens, Greece Roy R., Dept. of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA Ruman T., Rzeszów University of Technology, Faculty of Chemistry, Bioorganic Chemistry Laboratory, Rzeszów, Poland Rundqvist H., Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden Rutteman G.R., Univ. Clinic of Companion Animals, Utrecht and Veterinary Specialist Center De Wagenrenk, Wageningen, The Netherlands Saal L., Translational Oncogenomics Unit, Department of Oncology, Lund University, Lund, Sweden Sadaoui I., Department of Medical Genetics Faculty of Medicine and Pharmacy, Casablanca, Morocco Saeki H., Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Sakaeva D., Department of Pharmacology, Bashkir State Medical University, Bashkir, Russia Salaroglio I.C., Department of Oncology, University of Turin, Turin, Italy Salim E.I., Cell, Genetics & Molecular Carcinogenesis, Tanta University, Faculty of Science, Tanta, Egypt Salvioni R., SC Urology Foundation IRCCS, National Cancer Institute of Milan, Milan, Italy Sandoval-Acuña C., Lab. de Metabolismo Energético y Cáncer, Programa de Farmacología Molecular y Clínica, ICBM, Fac. de Medicina, Univ. de Chile, Santiago, Chile Sansom C., Department of Biological Sciences, Birkbeck, University of London, London, UK Saponara S., Department of Life Sciences, University of Siena, Siena, Italy Sarkar S., Cancer Center, Department of Medicine, Boston University School of Medicine, Boston, MA, USA 5780

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Sasiadek M.M., Department of Genetics, Wroclaw Medical University, Wroclaw, Poland Satapathy S.R., Cancer Biology Division, KIIT School of Biotechnology, KIIT Univ., Odisha, India Scarlat A., Fundeni Clinical Institute, Centre of General Surgery and Liver Transplantation, Bucharest, Romania Scatena R., Institutes of Biochemistry and Clinical Biochemistry, School of Medicine, Catholic Univ. of the Sacred Heart, Rome, Italy Schauer M., Klinik für Allgemein-, Viszeral- und Kinnderchirurgie university Klinik Düsseldorf, Düsseldorf, Germany Schiffer D., Neuro-bio-oncology Center, Policlinico di Monza Foundation/Consorzio di Neuroscienze, Univ. of Pavia, Vercelli, Italy Schneider J.A., Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Alcorcón (Madrid), Spain Schneider M., FB C − Organische Chemie, Bergische Universität Wuppertal, Wuppertal, Germany Scialpi M., Department of Surgical and Biomedical Sciences, Division of Radiology 2, Perugia University, S. Maria della Misericordia Hospital, Perugia, Italy Scieglinska D., Center for Translational Research and Molecular Biology of Cancer, M. Sklodowska-Curie Memorial Cancer Center and Inst. of Oncology, Gliwice Branch, Gliwice, Poland Sedloev T., Department of Surgery, University Hospital “Queen Joanna”, Sofia, Bulgaria Seidman E.G., Division of Gastroenterology, Research Institute, McGill Univ. Health Center, Faculty of Medicine, McGill Univ., Montreal, QC, Canada Semelakova M., Dept. of Cell and Mol. Pharmacology and Experimental Therapeutics, Medical Univ. of South Carolina, Charleston, SC, USA Serna-Márquez N., Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Dept. de Biología Celular, D. F., México Seth A., Sunnybrook Research Institute, Toronto, ON, Canada Sgaragli G., Department of Life Sciences, University of Siena, Siena, Italy Shah B., Cancer Center and Blood Institute, St Joseph Regional Medical Center, Lewiston, ID, USA Shany S., Dept. of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion Univ. of the Negev, Beer-Sheva, Israel Sharoni Y., Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion University, Beer-Sheva, Israel Sharvit L.E., Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, Haifa, Israel Shepard H.M., Halozyme Therapeutics, Inc. Sorrento Valley Rd., San Diego, CA, USA Sica G., Institute of Histology and Embryology, “A. Gemelli” Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy Sideris M., Clinical Research Fellow in Colorectal Surgery, King’s College Hospital, NHS Foundation Trust, London, UK Singh P.K., The Eppley Institute for Cancer and Allied Diseases, Univ. of Nebraska Medical Center, Omaha, NE, USA Sinha A.A., Departments of Urology, Genetics, Cell Biology & Development, Univ. of Minnesota, Minneapolis, MI, USA Sinzinger H., ISOTOPIX, Institute of Nuclear Medicine, Vienna, Austria Slansky J.E., University of Colorado School of Medicine, Denver, CO, USA Sliva D., Cancer Research Lab., Methodist Research Inst., Indiana Univ. Health, Indianapolis, IN, USA Sliwinski T., Department of Molecular Genetics, University of Lodz, Lodz, Poland Śliwka L., Medical University, Faculty of Pharmacy with the Laboratory Medicine Division, Dept. of Bioanalysis and Analysis of Drugs, Warsaw, Poland Smetana Jr K., Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic Smith D., Macro Innovations Ltd, Cambridge, UK Smith R.J., Macro Innovations Ltd, Cambridge, UK Smith S., City of Hope, Duarte, CA, USA Smits E.L., Laboratory of Experimental Hematology, University of Antwerp, Antwerp, Belgium Snapka R.M., Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA Sobol R.W., The Hillman Cancer Center, Pittsburgh, PA, USA Sokolowska J., Department of Morphological Sciences, Fac. of Veterinary Medicine, Warsaw Univ. of Life Sciences - SGGW, Warsaw, Poland Sorokin A., Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA 5781

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Spengler G., Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary Spitz J., Institute for Medical Information and Prevention, Schlangebad, Germany Spyridopoulou K., Depts. of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece Spyropoulos D.D., Department of Pathology, Medical University of South Carolina, Charleston, SC, USA Stanciulea O., Fundeni Clinical Institute, Bucharest, Romania Starok M., Université de Technologie de Compiègne, GEC, CNRS, Compiègne, France Stec W.J., Celther Polska, Lodz, Poland Stepulak A., Medical University in Lublin, Department of Biochemistry and Molecular Biology, Lublin, Poland Stölting D.P., Institute of Pharmacy, Rheinische Friedrich Wilhelms University, Bonn, Germany Stope M.B., Department of Urology, University Medicine Greifswald, Greifswald, Germany Storey A., Department of Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK Strakova N., Dept. of Cytokinetics, Inst. of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Studenovsky M., Inst. of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic Su C.-C., Tumor Res. Center of Integrative Medicine, Comprehensive Breast Cancer Center, Dept. of Surgery, Changhua Christian Hospital, Changhua City, Taiwan, ROC Sugie S., Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake-cho, Miyazaki, Japan Sultanbaev A., Republican Clinical Oncology Dispensary, Ufa, Russia Syed A., Institute for Integrated Micro and Nano Systems, the University of Edinburgh, Edinburgh, UK Syrjänen K.J., Biohit Oyj, Helsinki, Finland Syrjänen S., Dept. of Oral Pathology, Inst. of Dentistry, Faculty of Medicine, University of Turku, Turun Yliopisto, Finland Szabó A.M., Dept. of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary Szabó D., Departments of Oto-Rhino-Laryngology and Head & Neck Surgery, University of Szeged, Szeged, Hungary Szende B., Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary Szendi K., Dept. of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary Tadmor T., Hematology Unit, Bnai-Zion Medical Center, Haifa, Israel Tajima K., Dept. of Public Health and Occupational Medicine, Mie University Graduate School of Medicine, Mie, Japan Takayama T., Department of Digestive Surgery, Nihon University School of Medicine, Tokyo, Japan Tarasiuk J., Dept. of Biochemistry, Faculty of Biology, University of Szczecin, Szczecin, Poland Tarle M., University Clinical Hospital Center Sestre Milosrdnice, Zagreb, Croatia Tateishi R., Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan Taylor R.E., Warren Family Center for Drug Discovery and Development, Dept. of Chemistry & Biochemistry, Univ. of Notre Dame, Notre Dame, IN, USA Tchevkina E., N.N.Blokhin Russian Cancer Research Center, Moscow, Russia Theophanides T., National Technical University of Athens, Chemical Engineering Department, Athens, Greece Thomas W., Molecular Med. Laboratories, Royal College of Surgeons in Ireland, Educ. and Research Centre, Beaumont Hospital, Dublin, Ireland Thompson A., Centre for Cancer Research and Cell Biology, Queen’s University, Belfast, Northern Ireland Tibold A., Department of Public Health, University of Pécs, Medical School, Pécs, Hungary Tikhomirova I., Department of Medicine, Yaroslavl State Pedagogical University, Yaroslavl, Russia Tilki G., Harmonisation psychique et gestion du surstress par les Élixirs Floraux, Avignon, France Timirci Kahraman O., Dept. of Molecular Medicine, Institute of Experimental Medicine, Istanbul Univ., Istanbul, Turkey Todorova R., IBPhBME-BAS, Sofia, Bulgaria Toma-Dasu I., Medical Radiation Physics, Stockholm University and Karolinska Institutet, Stockholm, Sweden Topolcan O., Immunoanalytical laboratory, University Hospital and Charles University in Plzen, Plzen, Czech Republic Torres-Reyes L.A., División de Inmunología, Centro de Investigación Biomédica de Occidente, IMSS, Guadalajara, Jalisco, Mexico Townsend P., Faculty Inst. for Cancer Sciences, Manchester Academic Health Sciences Centre, Univ. of Manchester, Manchester, UK Tręda C., Celther Polska Sp. z o.o., Łódź, Poland Trendowski M., Department of Biology, Syracuse University, Syracuse, NY, USA Treska V., Dept. of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic 5782

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Trigo L., Brachytherapy Service Department Radiotherapy, Instituto Português Oncologia, Porto, Portugal Troppmair J., Daniel Swarovski Research Laboratory, Dept. of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria Tsai C.-W., Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC Tsalihin D., Ben Yair Community Clinic, Beer-Sheva, Israel Tsalihin N., Ben Yair Community Clinic, Beer-Sheva, Israel Tsoukalas N., Department of Medical Oncology, 401 General Military Hospital, Athens, Greece Tunteeratum A., Division of Medical Genetics & Molecular Medicine, Dept. of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand Tuyaerts S., Division of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven, Belgium Twardziok M., Dept. of Pediatric Oncology/Hematology, Otto-Heubner-Center for Pediatric and Adolescent Medicine, Charité, Universitätsmedizin, Berlin, Germany Tzeveleki I.D., General Surgeon, Interbalkan Medical Center, Thessaloniki, Greece Ulukaya E., Medical Faculty of Uludag University, Department of Clinical Biochemistry, Bursa, Turkey Umamaheswaran G., Pharmacogenomics Laboratory, Department of Pharmacology, JIPMER, Pondicherry, India Umansky V., Skin Cancer Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany Urbanska K., Division of Histology and Embryology, Dept. of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw Univ. of Life Sciences, Warsaw, Poland Utispan K., Faculty of Dentistry, Thammasat University, Bangkok, Thailand Uzuelli J.A., Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, SP, Brasil Van De Ven W., KU Leuven Research & Development, Leuven, Belgium Vasileva M., Department of Surgery, Medical University of Sofia, Aleksandrovska University Hospital, Sofia, Bulgaria Vayssade M., Université de Technologie de Compiègne, BMBI, CNRS UMR 7338, Compiègne, France Vesely D.L., University of South Florida Morsani School of Medicine, Tampa, FL, USA Vetvicka D., Inst. of Biophysics and Informatics, First Faculty of Medicine, Charles Univ. in Prague, Prague, Czech Republic Vijayan R., Department of Biology, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates Vilkaitis G., Institute of Biotechnology, Vilnius University, Vilnius, Lithuania Viovy J.-L., Institut Curie, Paris, France Vlaykova T., Department of Chemistry Medical Faculty, Trakia University, Stara Zagora, Bulgaria Vollmer G., Department of Biology, Technische Universität Dresden, Dresden, Germany Vourtsi A., Hellenic Breast Imaging Society, Athens, Greece Waldum H.L., Faculty of Medicine, Norwegian University of Technology and Science, Dept. of Gastroenterology and Hepatology, St. Olavs Hospital, Trondheim, Norway Wan H., Barts and The London, School of Medicine and Dentistry, Centre for Clinical and Diagnostic Oral Sciences, Institute of Dentistry, London, UK Wang H.-C.R., Dept. of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, Univ. of Tennessee, Knoxville, TN, USA Wang Q.-E., Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA Wang Q., School of Healthcare Science, Manchester Metropolitan University, Manchester, UK Wang S.-C., Dept. of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Taichung Armed Forces General Hospital, Taichung, Taiwan, ROC Wang W., University of Wolverhampton, Wolverhampton, UK Wani A.A., Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA Ward E., Macro Innovations Ltd, Cambridge, UK Warenius H.M., University of Southampton, Faculty of Medicine, Southampton General Hospital, Southampton, UK Weber W., Medical Oncology, Basel, Switzerland Westrøm S., Oncoinvent AS, Oslo, Norway Williamson K., Centre for Cancer Research and Cell Biology, Queen’s University, Belfast, Northern Ireland Witz I., Department of Cell Research & Immunology, The George S. Wise Faculty of Life Sciences, Tel-Aviv Univ., Tel Aviv, Israel 5783

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Woźniak M., Department of Pathology, Wrocław Medical University, Wrocław, Poland Wu C.-S., Chang Gung Molecular Medicine Research Center, Kwei-Shan Tao-Yuan, Taiwan, ROC Yamashita Y.-I., Dept. of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Yang C., Department of Mol. Biology Laboratory, Shanghai Sixth People’s Hosp., Shanghai Jiaotong Univ. School of Medicine, Shanghai, PR China Yang N., Hunan Provincal Cancer Hospital / Affilated Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China Yaylim I., Istanbul University, The Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkey Yefenof E., The Lautenberg Center of Immunology and Cancer Research, IMRIC, Hebrew Univ.-Hadassah Medical School, Jerusalem, Israel Yılmazer S., Department of Medical Biology, Istanbul University, Cerrahpasa Medical Faculty, Istanbul, Turkey Yip C.-H., Subang Jaya Medical Centre, Selangor, Malaysia Yisraeli J.K., Dept. of Developmental Biology and Cancer Research, IMRIC, Hebrew University-Hadassah Medical School, Jerusalem, Israel Yoneyama K., Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan Yoo K.-Y., Seoul National University College of Medicine, Seoul, Korea Yoshizumi T., Dept. of Surgery and Science, Graduate Sch. of Medical Sciences, Kyushu Univ., Fukuoka, Japan Young A.R., Kings College London, London, UK Yu L.-G., Dept. of Gastroenterology, Inst. of Translational Medicine, Univ. of Liverpool, Liverpool, UK Yumrutas O., Department of Medical Biology, Faculty of Medicine, Adıyaman University, Adıyaman, Turkey Zabel M., Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Zarbl H., Environmental and Occupational Health Sciences Institute, Piscataway, NJ, USA Zhamanbayeva G.T., Department of Biophysics and Biomedicine, Al-Farabi Kazakh National University, Almaty, Kazakhstan Zhang H., School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Zhang L., University Health Network, Toronto, ON, Canada Zhao W., Depts. of Pathology and Internal Medicine, Comprehensive Cancer Center at The Ohio State University, Columbus, OH, USA Zheng W., School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu Province, PR China Zhou M., Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Human Cancer Hospital, Changsha, PR China Ziolkowska B., Department of Pathology, Wroclaw Medical University, Wrocław, Poland Zoras O., Department of Surgical Oncology, University Hospital of Heraklion, Heraklion, Greece

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Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece

List of Speakers – Presenters Name

Abstract Session Number Number

Abdel-Mageed AB 1 Abderrahim H 2 Abu Freha N 360 Aggarwal BB 3 Aguilar-Lemarroy A 4 Ahmed FE 5 6 Ahmed FE Ahmed M 7 8 Ährlund-Richter L Aiba H 9 Akbal O 10 Akimov MG 11 Alaiya AA 12 Alaoui-Jamali MA 13 Alexandrova AY 14 Al-Khanbashi M 15 Allegrucci C 16 Allen D 17 Alpizar-Alpizar W 18 Althurwi S 19 Alvarado A 20 Alvarado A 21 Amaral L 22 Amemiya Y 23 Ammoun S 24 Amrani Hassani Joutei H 25 Amrani Hassani Joutei H 26 Amrani Hassani Joutei H 27 Anastassopoulou J 28 Anderson D 29 Anderson SM 30 Andersson R 31 Anner P 32 Annovazzi L 33 Antov GG 34 Aoki T 35 Apaydin E 36 Arfaoui A 37 Arimondo PB 38 Arosio P 39 Arrighi N 40 Arsenopoulou V 41 Atanasov AT 42 Atanasova S 43 Auge JM 44 Augsten M 45 Bacalbasa N 46 Bacalbasa N 47 Bai Y 48 Bajenova O 49 Bakalova R 50 Baker BJ 51 Banerji P 52 Banerji P 52

40 54 12 16 22P 28 22 47 22 47 45 59P 20 71 70 78 23 54 52 9 31P 43P 42 78 24 43P 43P 24 1 20 1 61 61P 48 41P 49 58P 42P 39 48 1 31 22P 46P 26 12 47 47 15 70 29P 68 16 16

Name

Bar-Shalom R Barak V Barak V Barak V Batistatou A Bau DT Bellini MF Bellini MF Bellini MF Bendas G Bernasconi M Berte N Berte N Betsou F Bhakat KK Bhaumik SR Bhushan A Bhushan A Bielecka AM Bireller ES Bjerkvig R Black JD Bölke E Bollito E Bonucci M Boren T Bose SR Bousquet G Braczkowski RS Braczkowski RS Braidwood L Branca JJV Branca JJV Branca JJV Bray LJ Bremer E Brenner W Briasoulis E Brodin O Bryukhovetskiy AS Buondonno I Bureau EA Butkiewicz D Capelli E Carlsson J Carvalho MI Carvalho MI Catalan M Ceausu AR Ceausu AR Červinková B Chambers TC Champeris Tsaniras S Chang WS

Abstract Number 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 522 99 100 101 102 103 104 105

Session Number 59P 26 77 25 35 19 39P 31 69P 71 59 5P 5P 54 3 21 59 39 59P 59P 32 50 64 37 29 31 28 64 61 60 59 48P 64P 33P 74 11 71 54 9 36 42P 59P 61P 48 57 40 22P 5P 74P 74P 27P 58 22P 26

Name Chang YS Characiejus D Charles RP Chechlinska M Check JH Chen CJ Chen YL Chen YL Chen ZF Chi A Chiariello M Chien SS Cho CH Ciafrè SA Ciarimboli G Cimpean AM Cimpean AM Ciortea R Cirimbei C Clarke R Collery P Coosemans A Cotter TG Csonka A D'Ambrosio SM Dabrowska M Dalan AB Dalianis T Damaskos C Dasu A Dasu A Dauksa A Davidson B De Bari B De Brabander JK De Braekeleer M De Braekeleer M De Grève J De Marchis ML De Meirleir K De Oliveira JT De Vogelaere K Deliu Z Deppert W Desnoyers G Dettling S Dilruba S Dima SO Dimitroulakos J Diorio C Douik H Dovas A Drozdowska D Duburs G

Abstract Number 715 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158

Session Number 2P 11 2 43 10 67 74P 59P 59 28 56 30P 5 65 29 76 49P 72P 47P 56 45 11 24 42P 21 1P 40 8 61 64 64 43P 34 60 69 30 30 63 40 60P 55 47P 40P 70 22P 32 42P 47 17 64 41 13 5P 45

5785

ANTICANCER RESEARCH 34: 5761-6258 (2014) Name

Abstract Session Number Number

Dudek AZ 159 Dufès C 160 Dumitrascu T 161 Dunn J 162 Dunn J 163 Dus-Szachniewicz K 164 Dus-Szachniewicz K 165 Efferth T 166 El Sharouni S 167 168 Engström W Engström W 169 Ensinger C 170 Errico MC 171 Etrych T 172 Evangelisti C 173 Fabbri F 174 Facoetti A 175 Farinha Garcao Nunes J 176 Farkouh A 177 Faustino-Rocha AL 178 Faustino-Rocha AL 179 Faustino-Rocha AL 180 Faustino-Rocha AL 181 Felsher DW 182 Fenical W 183 Ferda J 184 Ferda J 185 Ferreira MJU 186 Ferroni P 187 Fiala O 188 Fici P 189 Fields AP 190 Fillmore HL 191 Fkih M' hamed I 192 Fountzilas G 193 Fourkala EO 194 Frikha A 195 Frosini M 196 Fuchs D 197 Fuchsova R 198 Fuchsova R 199 Funel N 200 Gabius HJ 201 Gabius HJ 202 Gaje PN 203 Galindo-Hernández O 204 Ganapathi MK 205 Ganapathi R 206 Ganatra SH – Gandhi V – Gärtner F 207 Gazzano E 208 Georgieva E 209 Georgopoulos A 177 Gerwick W 210 Getoff N 211 Gezer U 212

5786

71 6 47 66 66 61 61P 17 11 15 3 61P 23 6 77 14 48 42 44 74 46P 39P 46P 2 68 60 49 42 43 27P 14 50 32 78P 73P 72 49P 38 1 27P 60P 59 18 18 74P 64P 72 72 69P 5 55 38 40P 44 69 22P 26

Name

Abstract Number

Ghotra PK 213 Giamas G 214 Gil J 215 Gil-Bernabe AM 216 Giorgio L 217 Gloushankova NA 218 Gocze K 219 Goffart N 220 Gombos K 221 Gonzalez-Hormazabal P 222 Gospodinova Z 223 Gostner JM 224 Goswami PC 225 Goswami PC 226 Goussia AC 227 Grisendi G 228 Gruia MI 229 Guadagni F 230 Guerra B 231 Gulubova M 232 Gulubova M 233 Gupta KP 234 Guttenplan JB 235 Guzinska-Ustymowicz K236 Guzinska-Ustymowicz K237 Hafizi S 238 Halasova E 239 Halasova E 240 Hamann MT 241 Harvey AJ 242 Haybaeck J 243 Haybaeck J 244 Haybaeck J 245 Haybaeck J 246 Haybaeck J 247 Hazra T 248 Helke KL 249 Hellevik T 250 Hellstrand K 251 Helson L 252 Hensler S 253 Herold Mende C 254 Herzog M 255 Hilakivi-Clarke L 256 Hoeschele JD 257 Hoffman RM 258 Hofmanova J 259 Holdenrieder S 260 Holst J 261 Hong TM 262 Horwitz SB 263 Hsu CM 264 Huang CY 265 Huang KB 266 Hunter K 267 Hunyadi A 268 Iacopino F 269

Session Number 61 57 1P 57 5P 13 78P 65 78P 30P 69P 40 13 22 35 57 59P 54 45 33P 1P 78 68 61P 61 71P 40P 40 79 39 29 1P 61P 20P 29P 21 29 62 77 7 71P 32 8 63 59 22 56 25 56 74P 69 30 30 59 23 42 40

Name Iacopino F Iacopino F Ibrahim FM Iglesias-Gato D Ikitimur-Armutak EI Ilhan N Ilhan N Illemann M Ilyas M Inan S Ingvarsson S Isbir T Isbir T Ishikawa M Ivanova K Jackson J Janeklang S Januchowski R Jassam SA Jave-Suárez LF Jean-Claude BJ Jernström H Jha G Jhanwar SC Jhanwar SC Jhanwar-Uniyal M Jhanwar-Uniyal M Jiang WG Johnson CE Judson I Juniku-Shkololli A Juršėnaitė J Kaetzel DM Kaina B Kaina B Kalamida D Kallay E Kallianos A Kalogera E Kamba AS Kanaan YM Kaneko H Kara G Karagounis IV Karanikas G Karlsson U Karydas I Kastler B Katanaev VL Kawasaki S Kayser K Keatley K Keisari Y Khalifa MA Khotimchenko MY Khotimchenko Y Kinkorova J

Abstract Number 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 738 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 – 317 318 319 320 321 322 323 324

Session Number 22P 38P 69P 19 59P 74P 31P 52 52 59P 30 41 41 33 13P 42P 69P 42P 32 58P 60 63 73 65 37 65 36 74 59P 49 44 33P 57 53 58 56 3 30P 27P 29 63 51 42 56P 43 28 35 62 22 51 18 32 10 72 69P 69P 27

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Name Kirti Kiss I Kiss Z Klapdor R Klapdor R Klein J Kleinsimon S Klump H Knutson KL Kobierzycki C Koch J Koeberle B Kohonen-Corish MRJ Konarikova K Konrad M Koontongkaew S Kopitz J Korhonen R Koukourakis MI Kowalewska M Kraemer S Kraemer S Kraemer S Książkiewicz M Kuang BH Kubista M Kubota K Kucera R Kuchmenko OB Kujovská Krcmová L Kumar S Kust D Laczmanska I Laerum OD Lage H Lamprecht S Lamprou DA Lamprou DA Lapini A Larsen RH Laube F Laube F Laurinavičius S Lazarou A Lazarou A Leitges M Lejeune FJ Leman E Levy J Li R Lichty BD Lintzeris I Lintzeris I Liotsiou T Liska V Liska V Lisowska KM

Abstract Session Number Number 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 739 369 370 371 372 373 374 375 376 377 378 379 380

41 41 29 25 9 14P 59P 23 64 72P 33 38 1 5P 5 13 18 64P 56 5P 76 64 76 2P 8 14 51 61 29P 26 74 49 1P 52 17 12 6 39 37 4 2P 2P 31P 72P 72 50 75 59P 34 79 10 47P 47P 49P 29P 29P 72P

Name Lissoni P Littlefield BA Liu YC Livney YD Lücke K Łukawska M Lumachi F Lunde MS Luqman S Mabeta P Maciejewski R Maeda H Mahfoud W Mahfoud W Man YG Mandal CC Mangiola A Manome Y Mantso T Mantso T Marchoudi N Martignoni G Martin SS Martinez-Zubiaurre I Martins A Martner A Martorana G Matakova T Mather R Matsumoto Y Matuschek C Mavroidi B Mazur L Mazure N McHale AP Mehta K Mellai M Messina G Micallef J Michael A Michelakis D Milczarek M Milczarek M Mirossay L Mishra R Mitra S Mitrakas AG Miyata K Miyata K Mohd Mutalip SS Mojzis J Mojzisová G Mol JA Molina R Molnár J Mooberry S Moore M

Abstract Number 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437

Session Number 29 79 59 17 14 45 61 19 69 74 11 6 75P 49 70 70 65 40 59 58 61 37 14 13 42 33 37 40P 32 45 64 59P 59 55 6 10 48 29 61 23 75 42P 45P 13P 40P 53 56P 40P 46P 24P 69P 59P 55 25 40 68 66

Name Moore M Morgan R Motaln H Mourad WF Mueller MM Muftuoglu S Muggia F Murakami N Murray NR Nagler R Nakamura K Nanni U Nano R Nano R Nano R Naor D Narayanan R Naz A Ngugen AV Nguyen NP Nguyen NP Nicolini C Nishikata T Nonni A Nowacki L Nutthasirikul N Ohno T Okamoto T Olbryt M Onal T Önder Mert NI Ontario M Ontario S Ooi H Orsós Z Osada S Osada S Ozay Y Ozgul M Ozturk M Pajkos G Palakurthi S Palmirotta R Panagiotidis M Pandita RK Pandita TK Papagerakis P Papagerakis S Papagrigoriadis S Pappagallo GL Paschos K Paschos K Pastwa E Patel MR Pathak S Pechkova E Penichet ML

Abstract Number 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494

Session Number 67 23 32 62 40 56P 9 73 50 61 70 54 48 48 48 12 19 1 40 28 28 20 33 35 79 61P 49 47 75P 58P 58P 54 54 43 44P 43P 49P 59P 24P 69 78P 7 54 49 21 21 73 73 47 37 70 71 39P 59 16 20 23

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ANTICANCER RESEARCH 34: 5761-6258 (2014) Name

Pentimalli F Peppa V Peredo-Silva L Perjési P Perjési P Pfankuchen D Phumyen A Pilkington GJ Pinchuk IN Piukovics K Plaimee P Plaimee P Pongracz JE Poplawski T Porta C Porta E Postema MHD Pottier A Pouliliou S Presl J Priel E Priel E Prinz C Provatopoulou X Provenzano L Pryczynicz A Queiroga FL Queiroga FL Queiroga FL Queiroga FL Radosevich JA Raica M Rakosy Z Ramachandran GK Ramonaite R Ramos LCB Ramqvist T Rapp C Rapti A Rashid S Ray A Ray BK Recchia F Restucci B Reyes-Uribe E Reyland ME Reynaud C Riboni L Richman SD Riondino S Rochel-Guiberteau N Rode W Roelant C Romo D Roos A Roselli M Rosi G

5788

Abstract Session Number Number 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551

78 40 39P 39P 34 38P 59 32 72 77P 59P 59P 74 38P 37 49P 68 49 49P 72 36 40 33 27 22P 71P 22P 22P 29P 40 36 76 64 75 78P 39P 43 32 49 46P 3 22 76 63 22P 50 55 40 61 44 59 40 49P 68 45 54 61P

Name

Rosi G Roupa I Roy R Ruman T Rundqvist H Rutteman GR Sadaoui I Sadaoui I Saeki H Sakaeva D Salaroglio IC Salim EL Salvioni R Sandoval-Acuña C Sansom C Sansom C Saponara S Sarkar S Sasiadek MM Satapathy SR Scarlat A Scatena R Schauer M Schiffer D Scieglinska D Sedloev T Seidman EG Semelakova M Serna-Márquez N Seth A Seth A Sgaragli G Shah B Shany S Sharoni Y Sharvit LE Shepard HM Sica G Sica G Sica G Sica G Sica G Sideris M Singh PK Sinha AA Sinzinger H Slansky J Sliva D Sliwinski T Sliwka L Smetana Jr K Smetana Jr K Smith RJ Smith S Smits EL Snapka RM Sobol RW

Abstract Number 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608

Session Number 61P 59P 53 45 55 31 60 60 47 49P 42 67 37 5 61 61 38 45 40 58P 43P 36 57 48 38P 64P 29 71P 22P 2 41P 38 77 5 34 69P 13 65 13 36 74 74 2 55 60 43 63 60 75P 59P 12 18 45 1 33 21 21

Name

Sokolowska J Sokolowska J Sorokin A Spengler G Spengler G Spitz J Spyridopoulou K Spyridopoulou K Spyridopoulou K Spyropoulos DD Stanciulea O Starok M Stec WJ Stepulak A Stölting BP Stope MB Storey A Storey A Strakova N Studenovsky M Su CC Sugie S Syed A Syrjänen KJ Syrjänen S Szabó AM Szabó D Szende B Szendi K Tadmor T Tajima K Takayama T Tarasiuk J Tarasiuk J Tarle M Tateishi R Taylor R Tchevkina E Theophanides T Thomas W Thompson A Tibold A Tikhomirova I Timirci Kahraman O Todorova R Toma-Dasu I Topolcan O Torres-Reyes LA Townsend PA Tręda C Trendowski MR Treska V Trigo L Trigo L Troppmair J Tsai CW Tsoukalas N

Abstract Number 609 610 611 612 613 614 615 616 617 618 619 620 621 622 623 624 625 626 627 628 629 630 631 632 633 634 635 636 637 638 639 640 641 642 643 644 645 646 647 648 649 650 651 652 653 654 655 656 657 658 659 660 661 662 663 664 665

Session Number 77P 77P 2 46P 42 44 69P 46P 79 13 47 69 22P 22 38P 42 58 58 45 6 45 60P 6 27 73 38P 13P 58 44 77 67 51 42 42P 60 51 69 40 8 2 23 78P 72 41P 24 60 27 22P 76 2P 7 7 62 64 17 20 43P

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Sithonia, Greece Name Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tsoukalas N Tunteeratum A Tunteeratum A Tuyaerts S Twardziok M Tzeveleki I Ulukaya E Umamaheswaran G Umansky V Urbanska K Urbanska K Utispan K Uzuelli JA Vasileva M Vesely DL

Abstract Session Number Number 666 667 668 669 670 671 672 673 674 675 676 677 678 679 680 681 682 683 684 685 686 687 688 689

43P 40P 43 47 43P 27 27P 43P 43P 43P 30P 30P 33 59P 8 59 76 12 20P 22P 22P 59P 27P 4

Name Vetvicka D Vijayan R Vilkaitis G Viovy JL Vlaykova T Vollmer G Vollmer G Vourtsi A Waldum H Wan H Wang HCR Wang QE Wang Q Wang SC Wang W Wani AA Warenius HM Weber W Weber W Weber W Westrøm S Williamson K Witz IP Woźniak M

Abstract Number 690 691 692 693 694 695 696 697 698 699 700 701 702 703 704 705 706 707 708 709 710 711 712 713

Session Number 6 69P 78P 14 41P 44 68 35 31 22 31 21 40 30 45 21 39 41 34 41 39 34 12 24

Name Woźniak M Wu CS Yamashita YI Yang C Yefenof E Yilmazer S Yip CH Yisraeli JK Yoneyama K Yoo KY Yoshizumi T Young AR Yu LG Yumrutas O Yumrutas O Zarbl H Zhamanbayeva GT Zhang HJ Zhao W Zheng W Zhou M Ziolkowska B Ziolkowska B Zoras O

Abstract Number 714 715 716 717 718 719 720 721 722 723 724 725 726 727 728 729 730 731 732 733 734 735 736 737

Session Number 59P 2P 47 39P 12 75P 66 1 27P 66 47 15 71 5P 5P 63 69P 79 2 4 24 56 46P 75

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Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Porto Carras, Sithonia, Greece

1 STEM CELL-BASED THERAPY: A DOUBLE-EDGED SWORD?

Z.Y. Abd Elmageed, Y. Yang, R. Thomas, M. Ranjan, D. Mondal, K. Moroz, B. Rezk, K. Moparty and A.B. Abdel-Mageed

Tulane University School of Medicine, New Olreans, LA, USA

Background: Emerging evidence established a link between adiposity (BMI >25 kg/cm2) and risk for prostate cancer (PC) progression and poor clinical outcome. Adipose tissue derived mesenchymal stem cells (ASCs), which dwell in perivascular niche of fat tissues, are often recruited to and promote tumor growth by unknown mechanisms. The objective of the study was to unravel the underlying mechanisms by which ASCs promote PC growth. Materials and Methods: ASCs were isolated from fat depot of PC patients (pASCs) undergoing radical prostatectomy. Tumor-tropic ASCs were enriched by a transwell system and FACS. The ability of PC cell-derived conditioned media (CM) or exosomes to induce neoplastic reprogramming of pASC was investigated in vivo. The mechanistic role of tumor-derived exosome ‘cargo” in oncogenic reprogramming of pASCs was examined in vitro and in vivo. Results: Unlike normal ASCs, the pASCs primed with PC cell CM formed prostate-like neoplastic lesions in vivo and reproduced aggressive tumors in secondary recipients. The pASC tumors acquired cytogenetic aberrations and mesenchymal-to-epithelial transition and expressed epithelial, neoplastic and vasculogenic markers reminiscent of molecular features of PC tumor xenografts. Our mechanistic studies revealed that PC cell-derived exosomes are sufficient to recapitulate formation of prostate tumorigenic mimicry generated by CM-primed pASCs in vivo. This was associated with down-regulation of tumor suppressors and trafficking of oncogenic factors into pASCs by exosomes, including H-ras and K-ras transcripts, oncomiRNAs and the Ras superfamily of GTPases. Conclusion: Our data suggest that PC cell microenvironment subverts PC patient-derived stem cells to undergo neoplastic transformation. Together, our findings propose new concepts for tumor phenotypic variations in epithelial and mesenchymal states and provide novel therapeutic insights for tumor-derived MV-ASC axis in neoplastic outgrowth and metastasis in cancer patients. 2 QATAR BIOBANK, A COMPREHENSIVE DATA AND SAMPLE RESOURCE TO SUPPORT FUTURE HEALTHCARE INITIATIVES H. Abderrahim

Qatar Biobank, Qatar Foundation, Doha, Qatar

Qatar Biobank has been established in order to enable medical research on health issues prevailing in Qatar. It is set as a joint initiative by Qatar foundation, Hamad Medical Corporation and the Supreme Council of Health, with the expert support of scientists from the Imperial College London. The pilot has a phase focused on the establishment of procedures and quality systems allowing to collect a very thorough set of samples, as well as lifestyle and dietary information from the participants. This effort is set to increase over the next 5 years to reach 60,000 participants from the Qatari adult population. In addition to providing a collection of valuable samples for research, this resource will constitute a reference for an epidemiological evaluation, as well as a longitudinal followup of the participants. 3 ANTI-INFLAMMATORY LIFE STYLE FOR PREVENTION AND TREATMENT OF CANCER: LET FOOD BE THY MEDICINE AND MEDICINE BE THY FOOD Bharat B. Aggarwal and Amit K. Tyagi, Lokesh Deb and Sahdeo Prasad

Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA

Chronic infections, obesity, alcohol, tobacco, radiation, environmental pollutants, and high-calorie diet have been recognized as major risk factors for the most chronic diseases including cancer. All these risk factors are linked to chronic diseases through inflammation. While acute inflammation that persists for short-term mediates host defense against infections, chronic inflammation that lasts for long-term can predispose the host to various chronic illnesses, including cancer. Linkage between cancer and inflammation is indicated by numerous lines of evidence; first, transcription factors NF-κB and STAT3, two major pathways for inflammation, are activated by most cancer risk factors; second, an inflammatory condition precedes most cancers; third, NF-κB and STAT3 are constitutively active in most cancers; fourth, hypoxia and acidic conditions found in solid tumors activate NF-κB; fifth, chemotherapeutic agents and gamma-irradiation activate NF-κB and lead to chemoresistance and radioresistance; sixth, most gene products linked to inflammation, survival, proliferation, invasion, angiogenesis, and metastasis are regulated by NF-κB and STAT3; seventh, suppression of NF-κB and STAT3 inhibits the proliferation and invasion of tumors; and eighth, most chemopreventive agents mediate their effects through inhibition of NF-κB and STAT3 activation pathways (Figure). Thus suppression of these proinflammatory pathways may provide opportunities for both prevention and treatment of cancer. We will discuss the potential 5791

ANTICANCER RESEARCH 34: 5761-6258 (2014)

Figure, Abstract No. 3 of various dietary agents, also called nutraceuticals derived from spices, lentils, nuts, fruits, and vegetables; and agents from traditional Ayurvedic medicine in suppression of inflammatory pathways and their role in prevention and therapy of cancer and other chronic diseases.

1 Aggarwal BB, Yuan W, Li S, Gupta SC. Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: Identification of novel components of turmeric. Mol Nutr Food Res. 2013 Sep;57(9):1529-42. 2 Aggarwal BB. Targeting Proteasomal Pathways by Dietary Curcumin for Cancer Prevention and Treatment. Curr Med Chem. 2013 Jun 25. [Epub ahead of print] 3 Gupta SC, Patchva S, Aggarwal BB. Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J. 2013 Jan;15(1):195-218. 4 Gupta SC, Sung B, Kim JH, Prasad S, Li S, Aggarwal BB. Multitargeting by turmeric, the golden spice: From kitchen to clinic. Mol Nutr Food Res. 2013 Sep;57(9):1510-28. 5 Gupta SC, Sung B, Prasad S, Webb LJ, Aggarwal BB. Cancer drug discovery by repurposing: teaching new tricks to old dogs. Trends Pharmacol Sci. 2013 Sep;34(9):508-17. 6 Aggarwal BB, Gupta SC, Sung B. Curcumin: an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers. Br J Pharmacol. 2013 Aug;169(8):1672-92. 7 Hasima N, Aggarwal BB. Cancer-linked targets modulated by curcumin. Int J Biochem Mol Biol. 2012;3(4):328-51. 5792

8 Sinha D, Biswas J, Sung B, Aggarwal BB, Bishayee A. Chemopreventive and chemotherapeutic potential of curcumin in breast cancer. Curr Drug Targets. 2012 Dec;13(14):1799819. 9 Yadav VR, Aggarwal BB. Curcumin: a component of the golden spice, targets multiple angiogenic pathways. Cancer Biol Ther. 2011 Jan 15;11(2):236-41. 10 Aggarwal BB, Sung B. NF-κB in cancer: a matter of life and death. Cancer Discov. 2011 Nov;1(6):469-71. 11 Gupta SC, Kannappan R, Reuter S, Kim JH, Aggarwal BB. Chemosensitization of tumors by resveratrol. Ann NY Acad Sci. 2011 Jan;1215(1):150-60. 12 Prasad S, Aggarwal BB. Turmeric, the Golden Spice: From Traditional Medicine to Modern Medicine. In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press; 2011. Chapter 13. 13 Yadav VR, Prasad S, Sung B, Aggarwal BB. The role of chalcones in suppression of NF-κB-mediated inflammation and cancer. Int Immunopharmacol. 2010 Dec 22. 14 Aggarwal BB, Sundaram C, Prasad S, Kannappan R. Tocotrienols, the vitamin E of the 21st century: its potential against cancer and other chronic diseases. Biochem Pharmacol. 2010 Dec 1;80(11):1613-31. 15 Aggarwal BB. Targeting inflammation-induced obesity and metabolic diseases by curcumin and other nutraceuticals. Annu Rev Nutr. 2010 Aug 21;30:173-99.

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Porto Carras, Sithonia, Greece

16 Chaturvedi MM, Sung B, Yadav VR, Kannappan R, Aggarwal BB. NF-κB addiction and its role in cancer: ‘one size does not fit all’. Oncogene. 2010 Dec 20. 17 Goel A, Aggarwal BB. Curcumin, the golden spice from Indian saffron, is a chemosensitizer and radiosensitizer for tumors and chemoprotector and radioprotector for normal organs. Nutr Cancer. 2010 Oct;62(7):919-30. 18 Gullett NP, Ruhul Amin AR, Bayraktar S, Pezzuto JM, Shin DM, Khuri FR, Aggarwal BB, Surh YJ, Kucuk O. Cancer prevention with natural compounds. Semin Oncol. 2010 Jun;37(3):258-81. 19 Gupta SC, Kim JH, Prasad S, Aggarwal BB. Regulation of survival, proliferation, invasion, angiogenesis, and metastasis of tumor cells through modulation of inflammatory pathways by nutraceuticals. Cancer Metastasis Rev.2010 Sep;29(3):405-34. 20 Gupta SC, Sundaram C, Reuter S, Aggarwal BB. Inhibiting NF-κB activation by small molecules as a therapeutic strategy. Biochim Biophys Acta. 2010 Oct-Dec;1799(1012):775-87. 21 Nair HB, Sung B, Yadav VR, Kannappan R, Chaturvedi MM, Aggarwal BB. Delivery of antiinflammatory nutraceuticals by nanoparticles for the prevention and treatment of cancer. Biochem Pharmacol. 2010 Dec 15;80(12):1833-43. 22 Prasad S, Phromnoi K, Yadav VR, Chaturvedi MM, Aggarwal BB. Targeting inflammatory pathways by flavonoids for prevention and treatment of cancer. Planta Med. 2010 Aug;76(11):1044-63. 23 Prasad S, Ravindran J, Aggarwal BB. NF-kappaB and cancer: how intimate is this relationship. Mol Cell Biochem. 2010 Mar;336(1-2):25-37. 24 Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med. 2010 Dec 1;49(11):1603-16. 25 Ralhan R, Pandey MK, Aggarwal BB. Nuclear factor-kappa B links carcinogenic and chemopreventive agents. Front Biosci (Schol Ed). 2009 Jun 1;1:45-60. 26 Ravindran J, Prasad S, Aggarwal BB. Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? AAPS J. 2009 Sep;11(3):495-510. 27 Sethi G, Sung B, Aggarwal BB. TNF: a master switch for inflammation to cancer. Front Biosci. 2008 May 1;13:5094107. 28 Shakibaei M, Harikumar KB, Aggarwal BB. Resveratrol addiction: to die or not to die. Mol Nutr Food Res. 2009 Jan;53(1):115-28. 29 Aggarwal BB, Danda D, Gupta S, Gehlot P. Models for prevention and treatment of cancer: problems vs promises. Biochem Pharmacol. 2009 Nov 1;78(9):1083-94. 30 Aggarwal BB, Gehlot P. Inflammation and cancer: how friendly is the relationship for cancer patients? Curr Opin Pharmacol. 2009 Aug;9(4):351-69.

31 Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009 Jan;41(1):40-59. 32 Aggarwal BB, Kunnumakkara AB, Harikumar KB, Gupta SR, Tharakan ST, Koca C, Dey S, Sung B. Signal transducer and activator of transcription-3, inflammation, and cancer: how intimate is the relationship?. Ann N Y Acad Sci. 2009 Aug;1171:59-76. 33 Aggarwal BB, Van Kuiken ME, Iyer LH, Harikumar KB, Sung B. Molecular targets of nutraceuticals derived from dietary spices: potential role in suppression of inflammation and tumorigenesis. Exp Biol Med (Maywood). 2009 Aug;234(8):825-49. 34 Aggarwal BB, Vijayalekshmi RV, Sung B. Targeting inflammatory pathways for prevention and therapy of cancer: short-term friend, long-term foe. Clin Cancer Res. 2009 Jan 15;15(2):425-30. 35 Aggarwal BB. Inflammation, a silent killer in cancer is not so silent! Curr Opin Pharmacol. 2009 Aug;9(4):34750. 36 Aggarwal BB, Sung B. Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets. Trends Pharmacol Sci. 2009 Feb;30(2):85-94. 37 Anand P, Sundaram C, Jhurani S, Kunnumakkara AB, Aggarwal BB. Curcumin and cancer: An "old-age" disease with an "age-old" solution. Cancer Lett. 2008 Aug 18;267(1):133-64. 38 Goel A, Jhurani S, Aggarwal BB. Multi-targeted therapy by curcumin: how spicy is it? Mol Nutr Food Res. 2008 Sep;52(9):1010-30. 39 Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as "Curecumin": From kitchen to clinic. Biochem Pharmacol. 2008 Feb 15;75(4):787-809. 40 Harikumar KB, Aggarwal BB. Resveratrol: a multitargeted agent for age-associated chronic diseases. Cell Cycle. 2008 Apr;7(8):1020-35. 41 Kunnumakkara AB, Anand P, Aggarwal BB. Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Lett. 2008 Oct 8;269(2):199-225. 42 Shishodia S, Harikumar KB, Dass S, Ramawat KG, Aggarwal BB. The guggul for chronic diseases: ancient medicine, modern targets. Anticancer Res. 2008 NovDec;28(6A):3647-64. 43 Aggarwal BB, Kunnumakkara AB, Harikumar KB, Tharakan ST, Sung B, Anand P. Potential of Spice-Derived Phytochemicals for Cancer Prevention. Planta Med. 2008 Oct;74(13):1560-9. 44 Aggarwal BB. The past, present and future of multi-targeted cancer treatment "Naturally": Food for thought. Cancer Lett. 2008 Oct 8;269(2):187-8. 5793

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45 Garodia P, Ichikawa H, Malani N, Sethi G, Aggarwal BB. From ancient medicine to modern medicine: ayurvedic concepts of health and their role in inflammation and cancer. J Soc Integr Oncol. 2007 Winter;5(1):25-37. 46 Jagetia GC, Aggarwal BB. "Spicing up" of the immune system by curcumin. J Clin Immunol. 2007 Jan;27(1):19-35. 47 Aggarwal BB, Sundaram C, Malani N and H. Ichikawa, “Curcumin: The Indian solid gold”, in The Molecular Targets and Therapeutic Uses of Curcumin in Health and Disease (ed by B.B. Aggarwal, Y-J. Surh, S. Shishodia), Springer Publishing Company, New York, 2007, p. 1-76. 48 Aggarwal BB. Nuclear factor-kappa B: a transcription factor for all seasons. Expert Opin Ther Targets. 2007 Feb;11(2):109-10. 49 Aggarwal, B.B. and Shishodia S., Surh Y-J (eds.) The Molecular Targets and Therapeutic Uses of Curcumin in Health and Disease Springer Publishers, New York, 2007 50 Aggarwal BB, Ichikawa H, Garodia P, Weerasinghe P, Sethi G, Bhatt ID, Pandey MK, Shishodia S, Nair MG. From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer. Expert Opin Ther Targets. 2006 Feb;10 (1):87-118. 51 Aggarwal BB, Sethi G, Ahn KS, Sandur SK, Pandey MK, Kunnumakkara AB, Sung B, Ichikawa H. Targeting signaltransducer-and-activator-of-transcription-3 for prevention and therapy of cancer: modern target but ancient solution. Ann N Y Acad Sci. 2006 Dec;1091:151-69. 52 Aggarwal BB, Shishodia S, Sandur SK, Pandey MK, Sethi G. Inflammation and cancer: how hot is the link? Biochem Pharmacol. 2006 Nov 30;72(11):1605-21. 53 Aggarwal BB, Shishodia S, Takada Y, Jackson-Bernitsas D, Ahn KS, Sethi G, Ichikawa H. TNF blockade: an inflammatory issue. Ernst Schering Res Found Workshop. 2006;(56):161-86. 54 Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol. 2006 71(10):1397-421. 55 Aggarwal, B.B. and Shishodia S., (eds.) Resveratrol in Health and Disease. Taylor and Francis Books, Inc., Boston, pp. 1-679, 2006 56 Ahn KS, Aggarwal BB. Transcription Factor NF-{kappa}B: A Sensor for Smoke and Stress Signals. Ann N Y Acad Sci. 2005 Nov;1056:218-33. 57 Shishodia S, Sethi G, Aggarwal BB Curcumin: getting back to the roots. Ann N Y Acad Sci. 2005 Nov;1056:206-17. 58 Garg AK, Buchholz TA, Aggarwal BB. Chemosensitization and radiosensitization of tumors by plant polyphenols. Antioxid Redox Signal. 2005 7(11-12):1630-47. 59 Dorai T, Aggarwal BB. Role of chemopreventive agents in cancer therapy. Cancer Lett. 2004 Nov 25;215(2):129-40. 60 Aggarwal BB. Nuclear factor-kappaB: the enemy within. Cancer Cell. 2004 Sep;6(3):203-8. 5794

61 Aggarwal BB, Bhardwaj A, Aggarwal RS, Seeram NP, Shishodia S, Takada Y. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res. 2004 Sep-Oct;24(5A):2783-840. 62 Aggarwal BB, Shishodia S. Suppression of the nuclear factor-kappaB activation pathway by spice-derived phytochemicals: reasoning for seasoning. Ann N Y Acad Sci. 2004 ;1030:434-41. 63 Aggarwal BB, Takada Y, Oommen OV. From chemoprevention to chemotherapy: common targets and common goals. Expert Opin Investig Drugs. 2004 Oct;13(10):1327-38. 64 Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res. 2003 Jan-Feb;23(1A):363-98. 65 Aggarwal BB. Signalling pathways of the TNF superfamily: a double-edged sword. Nat Rev Immunol. 2003 Sep;3(9):745-56. 4 EXPRESSION LEVELS OF WNT GENES IN CERVICAL CANCER-DERIVED CELL LINES: WNT7A IS DOWN-REGULATED AND ITS OVEREXPRESSION INHIBITS CELL PROLIFERATION A. Aguilar-Lemarroy1, M. Ramos-Solano1,2, I.D. Meza-Canales1, L.A. Torres-Reyes1,2, L. Alvarado-Ruíz1,2, M. Alvarez-Zavala1,2, P. Gariglio3 and L.F. Jave-Suarez1 1División

de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO)-Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico; 2Programa de Doctorado en Ciencias Biomédica, CUCS, Universidad de Guadalajara; 3Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, Mexico

Cervical cancer is one of the most common malignant tumors in women worldwide. Clinical, molecular, and epidemiological data have identified infection with Highrisk Human papillomavirus (HR-HPV) as a necessary cause for the development of this tumor; however, HR-HPV infection per se is not sufficient to generate a tumor. Recently, it has been proposed that the WNT pathway might be implicated in the malignant progression of keratinocytes immortalized with HR-HPV oncogenes. The WNT signaling pathway is a complex network of proteins involved in cell differentiation, proliferation, migration, and cell polarity and plays an important role during embryonic development, tissue regeneration, stem cell maintenance, and homeostasis.

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Porto Carras, Sithonia, Greece

In this study, we have investigated by microarray analysis and qPCR assays the expression of key components of WNT pathways to determine expression profile changes between non-tumorigenic keratinocytes and tumorigenic derivedcervical cancer cells. It could be demonstrated by qPCR that WNT7a is significantly reduced in cervical cancer-derived cell lines and in tissue biopsies from patients with cervical cancer when compared with HaCaT cells or normal cervical epithelial cells, respectively. Taking advantage of an inducible expression system and by using the xCELLigence platform to measure cell proliferation in real-time, it could be shown that WNT7a restoration in HeLa cells inhibit cell proliferation. Moreover, when WNT7a was silenced in non-tumorigenic HaCaT cells, cell proliferation accelerated strongly. Our results indicate that the expression of WNT7a plays a major role in the regulation of normal keratinocytes proliferation; thus, the inhibition of WNT7a expression appears to be an important step during cervical carcinogenesis. These findings could be important for generating further prognosis/diagnosis tools, or even anti-cancer therapies. At present, there is no report to our knowledge on the relation between WNT7a activity and cervical cancer.

5 STUDIES ON HUMAN CELLS IRRADIATED WITH TWO RADIATION MODALITIES (IMRT OR CONVENTIONAL RT) SHOW DIFFERENT MOLECULAR & CELLUAR RESPONSES: PUBLIC HEALTH IMPLICATIONS

Farid E. Ahmed1, Paul W. Vos2, Ron R. Allison3 and Clark Jeffries4 1GEM

Tox Labs, 2East Carolina Univ, 3Carolina Radiation Medicine, Greenville, NC, USA & Univ of North Carolina at Chapel Hill, NC, USA

We carried out this in vitro study to investigate the effect of two clinical X-irradiation modalities [a two-dimensional conventional external beam (RT) and a three dimensional conformal intensity-modulated radiation therapy (IMRT)] on a human epithelia cell line (colon adenocarcinoma HT-29) sensitive to ionizing radiation. Cells were synchronized by serum deprivation 48 h before irradiation so that >90% of them were in the G0/G1 phase of the cell cycle. Cells were allowed to recover 3 h after irradiation before total RNA extraction. Two types of arrays, namely Affymetrix Human HG U133A 2.0 oligonucleotide microarrays and Ambion mirVana bioarrays, were employed to study mRNA & microRNA expressions, respectively. Three flasks were used per irradiation dose, and an additional three unirradiated flasks served as control. Microarray data were validated by RTqPCR, and proteins of expressed genes were determined by

Western blots. Results showed the existence of differences in expression profiles between the two irradiation modalities. IMRT appeared to influence expression of some DNA repair genes, whereas in conventional RT some DNA repair and cell cycle-related genes that initially seemed to be preferentially expressed dwindled to normal levels. In vitro experiments using cell survival to study sublethal damage repair support our conclusions. Bioinformatic investigation revealed a correlation of gene expression with derepression effects of microRNA molecules. We propose opinions on how microRNAs influence gene expression during radiationinduced stress, and suggest a public health implication for this research. 6 RT-qPCR AND ITS APPLICATION TO MEDICINE & MOLECULAR BIOLOGY Farid E. Ahmed and Nancy C. Ahmed

GEM Tox Labs, Greenville, NC, USA

Reverse transcription, quantitative real-time PCR has simplified & enhanced quantification of gene expression by combining amplification and detection using advanced instrumentation, fluorescent chemistries & novel bioinformatics. Development of fluorescent-kinetic procedures in the early 1990s for analysis of nucleic acids enabled the rapid quantification of the PCR products during the exponential phase of the PCR reaction, requiring no post-PCR manipulations. There are two steps to this method: reverse transcription of messenger RNA or microRNA, and real-time PCR by a thermostable polymerase using a pair of synthetic oligonucleotide primers, each hybridizes to one of the opposite strand of a double strand nucleic acid target. PCR consists of 3 steps: denaturation at ~95˚C, primer annealing to a ssDNA at ~60˚C and primer extension at ~72˚C. A typical amplification cycle include 4 steps: baseline (or linear ground phase) that encompasses the first 15-20 cycles, early exponential phase where fluorescence reaches a threshold that is usually 10 times the SD of the base line (the cycle at which this occurs is the threshold cycle (Ct) or crossing point (CP), long-linear exponential phase, and the plateau phase that is reached when reaction components become limited due to their depletion, decline of polymerase activity or competition among PCR products. Different chemistries for detecting purified products exist, which are either nonspecific and uses intercalating dyes, or specific using various probes and hairpin structures. Two methods for product quantification exist: ‘absolute’ that uses serially diluted standards to generate a standard curve, or ‘relative’ to estimate the mean normalized gene expression from relative expression data and is used to correct for variation in material employed. Confounders and quality control procedures that could contribute to variable 5795

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results will be discussed, as well as modifications that allow for quantification of difficult to measure molecules such as microRNAs in stool obtained from normal and individuals with colon cancer.

7 MAGNETIC-GUIDED CANCER SURGERY Μ. Ahmed, Β. Anninga and Μ. Douek

Department of Research Oncology, King’s College London, London, UK

Background: Sentinel lymph node biopsy (SLNB) provides prognostic information in the management of solid tumors. Currently, the ‘combined technique’ using radioisotope and blue dye is considered the ‘gold standard’. This technique has drawbacks due to radioisotope dependence and adverse reactions to blue dye. Novel alternatives have been developed but the established ‘gold standard’ method for SLNB has not changed. Materials and Methods: We share our extensive clinical experience with the magnetic technique, which we developed as an alternative to radioisotope dependence for SLNB in breast cancer. We also discuss its application to other cancers and assess the published literature for current and potential future work. Results: The magnetic technique has been proven to be non-inferior to the combined technique for SLNB in breast cancer and consequently extended to concurrent breast lesion localization and SLNB in melanoma. Superparamagnetic iron oxide (SPIO) enhanced MRI has proven applications in axillary staging of breast cancer and could prove a viable, non-invasive alternative to SLNB. Conclusion: The magnetic technique has demonstrated promising results when applied to cancer surgery. Further assessment within well-constructed randomized controlled trials is now necessary to bring this innovative application into clinical practice. 8 A PHYSIOLOGICAL IN VIVO MODEL FOR NEUROECTODERMAL CHILD CANCER Isabell Hultman and Lars Ährlund-Richter

Karolinska Institutet. Department of Women’s and Children’s Health, Division of Paediatric Oncology, Stockholm, Sweden ([email protected])

Congruent with biological differences between childhood and adult cancers there is a need for new models, better employed, for studying tumors developing early in life. Considering the impact of microenvironment on tumor progression and aiming for a developmental match, we have tested pluripotent stem cell-induced teratoma (PSCT) as a homologous experimental

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in vivo niche for neuroectodermal child cancer. Mature benign PSCT, induced under standardized conditions from stem cells with normal karyotype, can be described as a failed embryonic process (1, 2) with a rich blend of tissues including early organoid development, as well as immature neural components. The immature components exhibit strong morphological resemblance with tumors of embryonic neuroectodermal origin and similar histology found in a nondevelopmental context would be considered potentially malignant. However, in the embryonic model such findings are part of early development (2). We have recently shown that this experimental situation provides matching microenvironment for the colonization and outgrowth of embryonic tumors transplanted directly from patients (3). The histology revealed a specific advantage over xenotransplantation showing a strict tropism with non-random integration of tumor growth into morphologically identifiable tissues and recuperating the patient tumor histology (4). 1 Gertow et al., Stem Cells Dev 13: 421-35, 2004. 2 PLoS ONE 6: e2774, 2011. 3 Jamil/Cedervall et al., Int J Oncol 43(3): 831-838, 2013. 4 Jamil et al., Int J Cancer 134: 1630-1637, 2014. 9 CONSERVATIVE TREATMENT FOR PATIENTS WITH OSTEOID OSTEOMA: A CASE SERIES

Hisaki Aiba1,2, Katsuhiro Hayashi1,3, Hiroyuki Inatani1,3, Yamada Satoshi1, Nobuyuki Watanabe2, Kiminari Sakurai2, Hiroyuki Tsuchiya3 and Takanobu Otsuka1

1Department

of Orthopaedic Surgery, Nagoya City University, Nagoya-city, Japan; 2Department of Orthopaedic Surgery, Tosei General Hospital, Seto-city, Japan; 3Department of Orthopaedic Surgery, Kanazawa University, Kanazawa-city, Japan

Background: Osteoid osteoma is one type of benign bone tumor that can respond to conservative treatment. Patients and Methods: A retrospective study of 11 patients with osteoid osteoma was performed. Initially, patients were treated with non-steroidal anti-inflammatories (NSAIDs), unless they continued to have intolerable pain or if the patient requested surgical resection and radiofrequency ablation (RFA). Results: Conservative therapy was successful for five patients. Three patients were treated with NSAIDs and the mean duration of pain was 22 months. The other two patients went into remission without medication by 9 and 24 months, respectively. Four patients underwent surgery after an average of 12 months. Two patients were treated with RFA after 47 and 11 months, respectively. Conclusion: Osteoid osteoma can be treated conservatively. Surgical resection and radiofrequency ablation should also be taken into

Abstracts of the 9th International Conference of Anticancer Research, 6-10 October 2014, Porto Carras, Sithonia, Greece

consideration as an option when the results of conservative treatment are poor.

10 PROTEIN AND POLYMERIC NANOPARTICLES FOR CANCER THERAPY: SYNTHESIS, CHARACTERIZATION, DRUG RELEASE AND INTERACTION WITH A BREAST CANCER CELL LINE (MCF-7) Öznur Akbal1,2, Ebru Erdal2, Tayfun Vural3, Doğa Kavaz4 and Emir Baki Denkbaş3

1Gazi

University, Polatlı Art and Science Faculty, Biology Department, Polatlı, Ankara, Turkey; 2Hacettepe University, Nanotechnology and Nanomedicine Division, Beytepe, Ankara, Turkey; 3Hacettepe University, Chemistry Department, Biochemistry Division, Beytepe, Ankara, Turkey; 4Cyprus International University, Department of Bioengineering, Haspolat, Nicosia, North Cyprus

Nanoparticles have the potential to improve the therapeutic index of drugs by increasing drug efficiency and lowering drug toxicity. Based on these reasons, the aim of the presented study was to prepare and characterize HSA (human serum albumin) and PHB-CMCh (polyhydroxy butyratecarboxymethyl chitosan) nanoparticles to be used in receptormediated cancer therapy and to compare their in vitro efficiency. HSA nanoparticles were prepared by the desolvation method and PHB-CMCh nanoparticles were prepared by a solvent evaporation method. Etoposide, a neoplastic agent, was loaded into nanoparticles and the nanoparticles’ surfaces were modified with a targeting ligand, concanavalin-A (Con-A). Size and morphological properties of nanoparticles were characterized by atomic force microscopy (AFM) and zeta sizer. Physicochemical properties were evaluated by Fourier transform infrared spectroscopy (FT-IR). Etoposide release profiles were obtained by UVvisible spectroscopy. The cytotoxicity of the HSA and PHBCMCh nanoparticles on the MCF-7 cell line were determined by using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) assay. As a result of all experiments, the size of HSA nanoparticles was determined as 102 nm and the size of PHB-CMCh nanoparticles was determined as 152 nm. For HSA nanoparticles, the etoposide loading ratio was between 52.6% and 80% depending on the amount of etoposide. For PHB-CMCh nanoparticles, the loading ratio was between 17.3% and 30.6%. The concentration of Con-A binding on the surface of nanoparticles was obtained as 73% for HSA nanoparticles and 65% for PHB-CMCh nanoparticles, respectively. Etoposide loaded and Con-A conjugated HSA nanoparticles had highly toxic effects on the MCF-7 cells and it was observed that

cytotoxicity depended on the increased concentrations of nanoparticles as it was 7.8% for 1 μg/ml and 61.1% for 100 μg/ml. On the other hand, etoposide loaded and Con-A conjugated PHB-CMCh nanoparticles had toxic effects on the MCF-7 cell line. The cytotoxicity was 78.4% for 1 μg/mL and 68.26% for 100 μg/ml.

11 SOME ASPECTS OF THE MECHANISM OF CELL DEATH INDUCTION BY THE LIPIDS OF N-ACYL DOPAMINE FAMILY IN THE PC12 CANCER CELL LINE

M.G. Akimov, A.M. Ashba, N.M. Gretskaya and V.V. Bezuglov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

The cytotoxicity mechanism of dopamine amides of arachidonic, docosahexaenoic, oleic and stearic fatty acids, as well as of arachidonic acid amides of tyramine, norepinephrine and 3-O-methyl dopamine was studied in PC12 cells. The substances were cytotoxic with LD50 in the range of 4-5 μM for N-acyl dopamines, 34±4 for N-arachidonoyl tyramine and 55±4 μM for N-arachidonoyl 3-O-methyl dopamine. The target of these substances was found to be the redox system. Introduction: N-Acyl dopamines (NADA) are biogenic conjugates of dopamine with long chain fatty acids. NADA were found in two species of the Hydra genus (1), in the nervous system of Rattus norvegicus and Bos taurus (2, 3) and in human plasma (4). The residues of fatty acids of the naturally occurring NADA encompass arachidonic, docosahexaenoic, oleic, palmitic and stearic acids (cf. (5) for a review). The antiproliferative and cytotoxic activity via an almost unknown molecular mechanism of N-acyl dopamines was found in several cell lines: MCF-7 (6), C6 (7), RAW264.7, WI-38, RBL-2H3, HeLa, HTB-126 (8), IMR-32, HOS, Namalwa, K-562 (9). The ability of short-chain NADA to induce cell death in SH-SY5Y neuroblastoma was also reported (10). The aim of this work was the elucidation of Nacyl dopamines cytotoxicity mechanism in the PC12 cell line. Materials and Methods: The rat pheochromocytoma PC12 cell line was from the Russian Collection of Cell Cultures (Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg, Russia). All test compounds were synthesized as previously described (11). Cells were maintained at 37˚C under 5% CO2 in RPMI 1640 medium supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 3.5 mg/ml D-glucose, 7% fetal bovine serum, 100 U/ml penicillin, 0.1 mg/ml streptomycin and 0.25 μg/ml amphotericin B. Attached cells were detached with 0.25% trypsin in 0.53 mM EDTA in Hanks’ salts. For analysis of toxicity of NADA and related compounds, the day before experiment cells were plated in 96 well plates at the density of 5797

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3×105 cells per well. Serial dilutions of test compounds prepared in DMSO and dissolved in the culture medium with or without the receptor blockers were added to cells in triplicate for each concentration and incubated for 20 h. Final DMSO concentration was 0.5%. Control cells were treated either with 0.5% DMSO (negative control) or 1% Triton X-100 (positive control). Separate controls were without DMSO (no difference with the control 0.5% DMSO were found). The effect of test substances on cell viability was evaluated using the MTT test (based on the MTT dye reduction by mitochondria of living cells) (12) with the removal of the compound-containig medium. Each experiment was repeated three times. Curves for calculating LD50 were generated using GraphPad Prism. Data are presented as mean±standard error. Data were compared using one-way ANOVA; p-values of 0.05 were considered significant. Results: To check, whether the toxic action of N-acyl dopamines and metabolically related molecules has some molecular targets, we used the rat pheochromocytoma PC12 cell line. PC12 possesses the key vanilloid receptor VR1, the second cannabinoid receptor CB2 and has enzymatic machinery to metabolize dopamine. Dopamine (DA) amides of arachidonic (AA), docosahexaenoic (DHA), oleic (Ol) and stearic (St) fatty acids, as well as of arachidonic acid amides of tyramine (TA), norepinephrine (NOR) and 3-O-methyl dopamine (3MDA) were studied. All N-acyl dopamines tested were toxic for PC12 cells with LD50 4±2 μM for Ol-DA, DHA-DA and AA-DA and 5±1 μM for StDA (difference was not statistically significant with p>0.9999; Figure 1). The modification of dopamine moiety to tyramine or 3-O-methyl dopamine led to a diminished toxicity (LD50 34±4 and 55±4 μM, respectively). The difference between the LD50 values of non-modified N-acyl dopamines and of the modified ones, as well as between the modified ones was statistically significant (p3060%). We point out this fact because it is a very important and crucial factor for precise analysis and interpretation of the experimental data obtained with hydrophobic anticancer substances. In our study, the final concentration of DMSO in the cell suspensions did not exceed 1%. Depending on the data processing approach we observed several tendencies. Based on the counting of the number of viable cells in each sample it was found that the drugs could be divided in three groups (Figure 1): (i) cytotoxic drugs (MLN-2238 type), which decrease continuously the number of viable cells in comparison to the initial level (1×106 viable cells/ml) within 72 hours of incubation; (ii) cytostatic drugs (BEZ-235 type), which have no negative effect on cell viability but stop their proliferation; in this case, the number of viable cells in the treated samples within 72 hours of incubation was almost the same as the initial level (1×106 viable cells/ml); (iii) drugs (PI-103 type) without effect on cell viability and with comparatively weak effect on cell proliferation (even at high 5825

ANTICANCER RESEARCH 34: 5761-6258 (2014)

Figure 1. Effect of 0.5 μM (A) and 5 μM (B) of MLN-2238, PI-103 and BEZ-235 on the number of viable cells in the cell suspensions at different time-intervals. Experimental conditions: Cells (Jurkat; 1×106 viable cells/ml) were incubated with anticancer drug (5 μM) in cell culture medium without antibiotics, in humidified atmosphere (37˚C, 5% CO2). At each time-interval, aliquots of the cell suspensions were subjected to cell viability assay using trypan blue staining and Countess™ Automated Cell Counter (Invitrogen). Data are mean±SD from six measurements. concentration – up to 25 μM); in this case, the number of viable cells in the treated samples was increased within 72 hours of incubation in the same way as in control (nontreated) cells. Based on the conventional approach for the calculation of cell viability as a percentage from the respective control, all drugs could be characterized as cytotoxic (Figure 2). Some of them are strongly cytotoxic as MLN-2238 and BEZ-235 (over 50% decrease of cell viability, even at low concentration: