... Inmaculada Jorge, Emilio Camafeita, Juan Antonio Lopez, Navratan Bagwan, ... Antonio Molina-Iracheta, Valentin Fuster, Borja Ibanez, Jesús Vázquez, Nat.
591 JACC April 5, 2016 Volume 67, Issue 13
Acute Coronary Syndromes PROTEOMIC TIME COURSE IN EARLY REMODELING AFTER MYOCARDIAL ISCHEMIA/REPERFUSION Poster Contributions Poster Area, South Hall A1 Sunday, April 03, 2016, 3:45 p.m.-4:30 p.m. Session Title: Acute Coronary Syndromes: Going One Step More Basic Abstract Category: 13. Acute Coronary Syndromes: Basic Presentation Number: 1214-005 Authors: Aleksandra Binek, Rodrigo Fernandez-Jimenez, Inmaculada Jorge, Emilio Camafeita, Juan Antonio Lopez, Navratan Bagwan, Carlos Galán, Jaume Aguero, Antonio Molina-Iracheta, Valentin Fuster, Borja Ibanez, Jesús Vázquez, Nat. Ctr. For Cardio. Research (CNIC), Madrid, Spain Background: We performed quantitative proteomics to uncover biological processes underlying early remodeling after myocardial ischemia/reperfusion (I/R).
Methods: Closed-chest 40 min I/R was performed in 20 pigs that were sacrificed at 120min, 24 hours, day 4 7 after I/R. Cardiac magnetic resonance (CMR) was performed at every follow-up until sacrifice when myocardial tissue samples were collected and processed for quantitative mass spectrometry proteomic analysis. Five pigs sacrificed after baseline CMR served as controls to compare with.
Results: In the ischemic myocardium (Panels A-D) we observed an upregulation of the inflammatory processes at all time points; while a significant increase in interstitial proteins, angiogenesis markers and cardio-renal signaling was observed at day 4 and 7 after I/R. In the remote myocardium (Panels E-H), early reperfusion (120min, 24h) affected proteins of the sarcomere, mitochondrial metabolism and energy transfer, which recovered to control values at day 7. These changes were accompanied by temporary decrease in systolic function in this area as assessed by CMR. Strikingly, we found an increase in proteins related to inflammation, regulation of body fluid levels, renal signaling and collagen at day 4 and day 7 in the remote area. Conclusions: Dynamic molecular changes are observed in remote and ischemic myocardium during the first week after I/R, providing insight into potential biomarkers for therapy and diagnosis.
