Additional file 3

Additional file 3: Sequences of the 95 species-specific oligonucleotides. Species

Abbreviation Oligonucleotide Sequence (5’-3’)

Boletus aestivalis Boletus calopus Boletus edulis Boletus erythropus Strobilomyces strobilaceus Xerocomus badius Xerocomus chrysenteron Xerocomus cisalpinus Xerocomus communis Xerocomus ferrugineus Xerocomus pruinatus Xerocomus subtomentosus Paxillus involutus Scleroderma citrinum Scleroderma verrucosum Suillus bovinus Suillus luteus Suillus variegatus Lactarius camphorata Lactarius chrysorrheus Lactarius deterrimus Lactarius hepaticus Lactarius quietus Lactarius subdulcis Lactarius theiogalus Russula betularum Russula brunneviolacea Russula cyanoxantha Russula densifolia Russula emetica Russula grisea Russula integra Russula nigricans Russula ochroleuca Russula parazurea

BOLAES BOLCAL BOLEDU BOLERY STRMSTR XERBAD XERCHR XERCIS XERCOM XERFER XERPRU XERSUB PAXINV SCLCIT SCLVER SUIBOV SUILUT SUIVAR LACTCAM LACTCHR LACTDET LACTHEP LACTQUI LACTSUB LACTTHE RUSBET RUSBRU RUSCYA RUSDEN RUSEME RUSGRI RUSINT RUSNIG RUSOCH RUSPAR

GAGTGTCATGGAATTCTCAACCGTGTCCTCGATCTGATCTCGAGGCATGGCTTGGACTTGGGAGTTGCTG TTGAGTGTCATCGAATTCTCAACCATGTCTCGATCTATTTCAAGGCATGGCTTGGAGTTGGGGGTTTGCT CCTGAAATGCATTAGCGATGTTCAGCAAGCCTGAACGTGCACGGCCTTTTCGACGTGATAACGATCGTCG TTGAGTGTCATTTGAATTCTCAACCATGTCTTGATTGATTTCGAGGCATGGCTTGGACTTGGGGGTTGCT GCAAAGACGTCGGCTCTCCTCAAACGCATGAGCGGGACTAGCATGTCCGGACGTG GAGGATCTATGTATTTCATCATCACACCTATCGTATGTCTAGAATGTCATCGTCGACCACTGGGCGGCGA GTGCACGTCCACCTTTCTCTTACTCTCACACCTGTGCACACATTGTAGGTCCTCGAAAGAGGATCTATGT GAAAGCGGTCGGCTCTCCTGAAATGCATTAGCAAAGGACAGCAAGTCTGACGTGCACGGCCTTGACG GACGTGATAATGATCGTCGTGGGCTGAAGCGTCGGACATGCATCGATTGTCTTGTTTGCTTCCAAATCAC CGTCCCCTCACCTTTTCTATCTACACACACCTGTGCACCTATTGTAGATCCCTCTCGAAAGAGAGGGAA TTCGCTGTGCACGTCTTTCTTTTCGTCGACCTTTCTCTTACTCTCACACCTGTGCACACACTGTAGGT CTTTCTTCTTTCTTGGATGGAAAGTATGGCTTGGAGTTGGGAGTTGCTGGCAGAGACTGTCAGCTCTCC GCCTTTCCTTTGGAAGACCCTTTCTTCACACCCGTGCACACATTGTAGGTCTCCGCGAGGGGATCTATGT GCATGTCTACAGAATGTCGTCCGTGGCGTCGGGCCACCGTAAACCATAATACAACTTTCAGCGATGGAT GAGTGTCATCGAAACCTCAGACCGACCCTTCGACCCCGTCGGAGCTCGGTCTGGACTTGTGGGAGTCTGC CAACTCCTCTCGATTGACTTCGAGTGGAGCTTGGATAGTGGGGGCTGCCGGAGACCTGAATATTCGTGTT CGGAGACACTGGATTCGTCCAGGACTCGGGCTCCTCTTAAATGAATCGGCTCGCGGTCGACTTTCGACTT GACCCGCGTCTTCATAAGCCCCTTCGTGTAGAAAGTCTATGAATGTTTTTACCATCATCGACTCGCGACT CGCTGGCTTTCAACGTTGTTGCACGCCGGAGCGTGTCCTCTCACATAACACAATCCATCTCACCCTTTGT CCTCTCACATAATAATCCATCTCACCCTTTGTGCACCACCGCGTGGGCACCCTTTGGGATC CGCTGACTTTTTGAGACACAAAAGTCGTGCACGCCGGAGTGCGTCCTCTCACATAAAATCCATCTCACCC GCAAGGGCTGTCGCTGACTCTATAAAGTCGTGCACGCCCGAGTGTGTCCTCTCACATAATAATCCATCTC CTTCTAATCGTCTCAACCTTGCATCGAGACAAACGTCTGAGCGTGGCTCCCTTCCCTGGGAAACTCTCTC GCTGTCGCTGACTCAAAGTCGTGCACGCCCGAGTGTGTCCTCTCACATAAATAATCCATCTCACCCTTTG GTCGTGAAAACCTCAACCTCTTTGGTTTCTTCTGGGGACCAAAGCAGGCTTGGACTTTGGAGGCCTTTTG GGTCATTTTCGACCGCGGAAAGGATTTTGGACTTGGAGGCCTTTTGCTGGTTTCACCTTGAAGCGAGCTC CTTTTTCTTTGACGAGAAAAGGAGTTTTGGACTTGGAGGTTCAATGCCCGCTTTCGGCATCGAAAGCGAG CTTCAACCTTTCTTGGTTTCTTGACCGAGGAAGGCTTGGACTTTGGGGGTCTTTCATTGCTGGCCTCTTT GTCGTGAATTTCTCAAACCTTCTTGGTTTCTTGATCAAGAAGGCTTTGGACTTTGGAGGTCTTTGCCGGC CTCCTCCCAAATGTATTAGTGGGGTCTGCATTGTCGGTCCTTGGCGTGATAAGTTGTTTCTACGTCTTGG GTGCATCACCGCGTGGGGCCCTTCTCTTTTCGGAGAGGGGGGTTCACGTTTTTACAAGAACGAACCATTA CCCTTTTTGTTTGAAAAGGATTTTTGGACTTGGAGGTTCCATGCTCGCCTTTGCTTTTGAAGGTGAGCTC GTCGTGAAATTCTCAAACCTTCTTGGTTCCTTGACCAAGATGGCTTTGGACTTTGGAGGCGTTGTGCTGG CCTTTTTCTTTTTGGGAAAGGGTTTTTGGACTTGGAGGCTTTTTGCTGGCTTCACCTTGAAGTGAGCTCC CCTTGACGTGATAAGTTTGCTTCTACGTCTTGGGTTTCGCACTGTCGCACCGGAACCTGCTTCCAACCGT

Russula puellaris Russula violeipes Amanita citrina Amanita crocea Amanita muscaria Amanita pantherina Amanita rubescens Laccaria amethystina Laccaria bicolor Laccaria laccata Strobilurus esculentus Tricholoma acerbum Tricholoma columbetta Tricholoma fulvum/ pseudonictitans Tricholoma populinum Tricholoma saponaceum Tricholoma sciodes Tricholoma ustale Cortinarius alboviolaceus Cortinarius anomalus Cortinarius bolaris Cortinarius cinnamomeus Cortinarius decipiens Cortinarius delibutus Cortinarius evernius Cortinarius hemitrichus Cortinarius hinnuleus Cortinarius multiformis Cortinarius privignus Cortinarius sanguineus Cortinarius sp 1 Cortinarius sp 2 Cortinarius sp 2 Cortinarius semisanguineus Cortinarius tortuosus Cortinarius traganus Cortinarius vulpinus Hebeloma radicosum

RUSPUE RUSVIO AMACIT AMACRO AMAMUS AMAPAN AMARUB LACCAME LACCBIC LACCLAC STRESC TRIACE TRICOL TRIFUL

CCTTTTGTGCATCACCGCGTGGGTCCCCCTTTGCGGGAGGGCTCGCGTTTTCACATAAAACTTGATACAG GCGTGGGCCACCTTCTTTGGCTTGTTTCAAAGAGGTCGGTTCACGTTTTTACACACACACACCTTTATG AGGTCCTTATGCAGCATGCAGGGAACTTTTGGACATTGGGAGTTGCTGGTCACTGATAAAGTGGCTGGCT CCTGTGCACCGCCTGTAGACACTCTGTGTCTATGATATATGTCACACACACACACACAGTTGTTTTAGGC GTCAAAACATGCACTTGAGTGTGTTTTGGATTGTGGGAGTGTCTGCTGGCTTTATGAGCCAGCTCTCCTG CCTGAAAGACATTAGCTTTGGAGGGATGTGCCAAGTCGCTTCTGCCTTTCCATTGGTGTGATAGACG TGGGATTTTTGGACATTGGGAGTTGCCGGCTGCTGATAAAGTGGTGGGCTCTTCTGAAAAGCATTAGTTG GGATACCTCTCGAGGCAACTCGGATTTTAGGGTCGCTGTGCTGTACAAGTCGGCTTTCCTTTCATTTCCA GCTTGGTTAGGCTTGGATGTGGGGGTTGCGGGCTTCATTAATGAGGTCGGCTCTCCTTAAATGCATTAGC GGATACCTCTCGAGGCAACTCGGATTTTAGGATCGCCGTGCTGCACAAGTCGGCTTTCCTTTCATTTCC CTTTGTACTCTTGTTGCTGTGTGCTGGCTTCTTCGGAAGTATGGTGCACGCTTGAGTGCAAGGGTCTTC AACCTTACTCAGCTTTCGCTAGTCGAGTTAGGCTTGGATATGGGAGTTTGTGGGCTTCTCGAAGTCGGCT CACTTTTATCGGTTGAATTAGGCTTGGATGTGGGAGTCTTTGCTGGCTTCGCAAGAGGTTGGCTCTCCTT CTACGCCATCATGTGAAGCAGCTTTAAATTGGGGTTGCTGCTCTCTAACAGTCTCTTTGGTGGGACAATT

TRIPOP TRISAP TRISCI TRIUST CORALB CORANO CORBOL CORCIN CORDEC CORDELI COREVE CORHEM CORHIN CORMUL CORPRI CORSAN CORSP1 CORSP2 CORSP3 CORSEM CORTOR CORTRA CORVUL HEBRAD

CCTAAAGTCGATCAGGCTTGGATGTGGGAGTTTGCGGGCTTTTCTAAAGTCGGCTCTCCTTAAATTT CCTTTTCAGCATTTATGTTGATCAGGCTTGGATGTGGGAGTTTGCGGGCTTCTCAGAAGTCGGCTCTCCT GACTTGGAATATCTCTAGAGGCAACTCGGTTTTGAGGATTGCTGTGCGCAAGCCAACTTTCCTTACAC CCTTTTCGGCTTTTTCTAAGTCGATTTAGGCTTGGATGTGGGAGTTTGCGGGCTTCTCTGAAGTCGGCTC CCTTCTCATTGCTGAGTGGTTTGGATGTGGGGGTTTGCTGGCCTCTTAAATGAGTTCAGCTCTCCTGAA CTTCAGCTTTTGCTTGTTGAGTGTTGGATGTGGGGGGTCTTTTGCTGGCCTTTTTTTAGAGGTCAGCTTC CTCCACCTGTGCACCTTTTGTAGACCTGAATAGCTTTCTGAATGCTAAGCATTCAGGCTTGAGGATTGAC CCAGGGTTTTTGACTTGTCGAGTGTTTGGATGTGGGGGTCTTTTGCTGGTCTCTTTTGAGGTCGGCTCCC GGGTTTGCTGGCCTTTTAAAAGGTTCAGCTCCTCTGAAATGCATTAGCAGAACAACCTTGCTCATTGGTG GAACAATTTGTTGACTGTTCATTGGTGTGATAATTATCTGCGCTATTGAACTGTGAGGCAAGTTCAGCTTC CTCCACCTGTGCACCCTTTGTAGACCTCCCAGGTCTATGTTGCTTCTTCATTTACCCCAATGTATGT CAAACCTTCTCTTTGTTGAGCGGTTTTGGATGTGGGGGTTTGCTGGCCTCTTAAAAAGGTTCAGCTCCTC CTAGGGAGCATGTGCACACCTTGTCATCTTTATATCTCCACCTGTGCACTTCTTGTAGGCCTTTCAGGT CTCCACCTGTGCACCTTTTGTAGACCTGGATATCTCTCTGAGTGCTTGCGCTCAGGTTTGAGGATTGATT CTTCTCATTGCTGAGTGGTTTGGATGTGGTGGTTTGCTGGCCTCTTAAATGAGTTCAGCTCTCCTGAATG CCTGTGCACCTTTTGTAGATCTGGATATCTTTCTGAATGCCTGGCATTCAGGTTTGGGGATTGACTTTGC GGGAGCATGTGCACGCCTTGTCATCTTTATATCTCCACCTGTGCACCTTTTGTAGACCCTTTCCAGGTCT CCTGATGGGTTGTTGCTGGTTCTCTGGGAGCATGTGCACACCTGTCATCTTTATATCTCCACCTGTGCAC GGGAGCATGTGCACGCCTGTCATCTTTATATCTCCACCTGTGCACTTTTGTAGACCTTCTGGGTCTATGT CTGGTCTCTTTTGAGATCGGCTCCCCTGAAATGCATTAGCGGAACAATTTGTTGACCCGTTCATTGGTG GCATGTGCACACCTGTCATCTTTATATCTCCACCTGTGCACCCTTTGTAGACCTTCTCAGGTCTATGTTG CAACCTTCTCTTGTTTGAGTGGTTTGGATGTGGGGGTTTGCTGGCTTCTTAAAAGGGTTCAGCTCCTCTG CAACCTCTTCAGCTTTTGCTTGTTGAGCGTTGGATGTGGGGGGTCTGTTTTGCTGGTCTTCTCAGGTCAG GCTTTTGTTGATACTGGCTTGGATATGGGGGTCTATTTTGCTGGCTTCTTTACAGATGGTCAGCTCCCC

Hebeloma sacchariolens Inocybe griseolilacina Inocybe napipes Inocybe sp Thelephora penicillata Thelephora terrestris Tomentella sublilacina Tomentellopsis submollis Cantharellus tubaeformis Clavulina cristata Hydnum repandum Clavariadelphus pistillaris Ramaria abietina Tylospora asterophora Tylospora fibrillosa Cenococcum geophilum Enthrospora colombiana Gigaspora margarita Glomus sinuosum Paraglomus occultum Scutellospora heterogama Arabidopsis thaliana

HEBSAC ISOGRI INONAP INOSP THEPEN THETER TOMSUB TOMPSUBM CANTUB CLACRI HYDREP CLAVPIS RAMABI TYLAST TYLFIB CENGEO ENTCOL GIGMAR GLOSIN PAROCC SCUHET ARATHA

CAGCTTTTGTTGATAACGGCTTGGATATGGGGGTTTTTTTTTGCTGGCTTCTTCACAGATGGTCAGCTCC GCTGTCCCTTCCTTTGGGTACGTGCACGCTTGTCATCTTTATTTCTACCCACTGTGCACATATTGTAGAC CTGCTGGCTCTCCTCGGAGGGCATGTGCACGCTTGTTGTCCATTATTTCTCCCACTGTGCACAAATTGTA GGCACGTGCACGCCTGTTTTTATTTGCTTCTCCAACTGTGCACAAATATCGTAGACCTTAGCAAGGCCTA AAATGAATCAGCTTGCCAGTCTTTGGTGGCATCACAGGTGTGATAACTATCTACGCTTGTGGTGGTC CTCTGTAGTTCTATGGTCTGGGGGACCCTGTCTTCCTTCTGTGGTTCTACGTCTTTACACACACACTGTA CTGGGGGACCCTGTCTTCCTGCCGTGGTTCTACGTCTTTACACACACTCTGTAATAAAGTCTTATGGAA GATCACGGAGCCCTGATGGGCAACGAATGCCCTCGTCTATGAATATTTTCACACACGCTCAAAGTATGAC CGGTCGCTTCCAATTGGGGGTTGACTCATAGGGGGTACATCTGTTTGAGGGTCATTTGTACCTTCTCAAA CACCTGTGCACATTTTTGAGGGAGTCTTGAGTTGGTTGCCGCTCTTGGGTGATTTTCTCACATTCCCTTA GGTATTCCGGGGAGCACACCTGTTCGAGTGTCATTGAAACTCTCAAATAAAGGTGGTTTTTGCAGACCAT GAGGAGCATGCCTGTTTGAGTGTCGTGAATCTCTCTCAATCCCACCTCTGTGGGCTTGGATTTGGATG GCATTAGCGTTCCGCCGCGAGTTCGGTTTCGTAACGACGGTGTGATAAGTAACACTTTGACGCCGTCTGG CCGAGCCCTTGAATCCCAAACACCACATGTGAACCCACCGTAGGCCTTCGGGCCTATGTCTTATCATATA CCCCCAACAAACACCGTGGGCCTTCGGGCCCGCGTATATTTACTCTGAATGTGTATAGAATGTAAACC GACGATTGACTCATGTTGCCTCGGCGGGCTCGCCCGCCAGAGGATACATCAAAAATCTTGTTTTAACGGT TGATCACCTCGCCGTCGATAGCTTTGCTAACCTCGGTGGGATCTGATTAACTAGAGATTAGACTGATCGT CCTTGATAGATGTGATGTTTGGGGTCGAGGATTGCAACGGATACCCCTTCGGGGCTAGCCGCCTGATCT CGTGGTGTTGCTTTTGTGACGCTTCGGAATTGGGTCATCTTGATCCTTTGGGTTAAGAGACT CACAAGTCCTCTGGAACGTGGCATCGTAGAGGGTGAGAATCCCGTCTCTGGTCGTTGTCTTGCAGGCA GTCAGCGTCGATTTTGGATATCATAAAATGATTGGGGGGAAGGTAGCTCCTTCGGGAGTGTTATAGCCCT AGCTTTTATCTCGGTCTTGTCGTGCGCGTTGCTTCCGGATATCACAAAACCCCGGCACGAAAAGTGTCA