Alginate Production by Plant-Pathogenic Pseudomonads

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Exopolysaccharides (EPS) of plant-pathogenic bacteria are thought to play an important role in both the ecology and the pathogenicity of the bacteria. EPS may ...
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 1986, p. 466-473

Vol. 52, No. 3

0099-2240/86/090466-08$02.00/0 Copyright © 1986, American Society for Microbiology

Alginate Production by Plant-Pathogenic Pseudomonads WILLIAM F. FETT,* STANLEY F. OSMAN, MARSHALL L. FISHMAN, AND T. S. SIEBLES III Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118 Received 18 February 1986/Accepted 22 May 1986

Eighteen plant-pathogenic and three non-plant-pathogenic pseudomonads were tested for the ability to produce alginic acid as an exopolysazcharide in vitro. Alginate production was demonstrated for 10 of 13 fluorescent plant-pathogenic pseudorAdonads tested with glucose or gluconate as the carbon source, but not for all 5 nonfluorescent plant pathogens and all 3 non-plant pathogens tested. With sucrose as the carbon source, some strains produced alginate while others produced both polyfructan (levan) and alginate. Alginates ranged from