Amniotic Membrane Transplant for Articular Cartilage ...

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The amniotic membrane (AM) or amnion is the innermost layer of the human placenta. It consists of a single layer of cuboidal cells, a thick basement membrane ...
Send Orders for Reprints to [email protected] Current Stem Cell Research & Therapy, 2014, 9, 00-00

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Amniotic Membrane Transplant for Articular Cartilage Repair: An Experimental Study in Sheep Diego Garcia1, Umile Giuseppe Longo2,*, Javier Vaquero3, Francisco Forriol4, Mattia Loppini2, Wasim Khan5 and Vincenzo Denaro2 1

Orthopaedic Surgery Department, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain; 2University Campus Bio-Medico of Rome, Italy; 3Orthopaedic Surgery Department, Hospital Universitario Gregorio Marañón, Madrid, Spain; 4University San Pablo – CEU, Campus de Montepríncipe, Boadilla del Monte, Madrid, Spain; 5University College London Institute of Orthopaedics and Musculoskeletal Sciences, Royal National Orthopaedic Hospital, Stanmore, United Kingdom Abstract: Purpose: The aim of the present study was to compare the potential for cartilage repair of fresh amniotic membrane (AM), cryopreserved AM and cryopreserved AM previously cultured with bone marrow mesenchymal stem cells (BM-MSCs) in an in vivo sheep animal model. Methods: A full-thickness cartilage defect was surgically produced in 12 adult sheep, in the bearing region of the lateral femoral condyle. The animals were randomized into 4 groups (n=3): no treatment of the defect (G1); filling with fresh AM (G2); with cryopreserved AM previously cultivated with BM-MSCs (G3); with cryopreserved AM alone (G4). Postoperatively, the full load was possible. At two months, the animals were euthanized. The quality of the new synthesized tissue was evaluated with the macroscopic, by using International Cartilage Repair Society (ICRS) scale, and histological analyses, by using O'Driscoll scale. Results: The control samples showed an ICRS grade III (abnormal); while the samples of Groups 2, 3 and 4 reported a grade II (similar to healthy cartilage). The mean value of O'Driscoll scale in the control group (3.3) was significantly lower compared to the treatment groups (G2: 10.7; G3: 8; G4: 11.3) (P