Poster abstracts. IV International Symposium on Animal Biology of Reproduction, October 17-20, 2012, Campinas, SP, Brazil.
ANIMAL REPRODUCTION Official journal of the Brazilian College of Animal Reproduction v.9, n.4
October/December
Effect of nutritional flushing on in vivo development of demi-embryos from superovulated Dairy Gir cows F.A. Oliveira1, C.A.A. Torres1, V.A. Nascimento2, J.M. Penitente Filho1, M.M.N.F. Oliveira3,
2012
Editors-in-Chief Eduardo L. Gastal - Southern Illinois University, Carbondale, USA Luiz Renato de França - UFMG, Brazil Co-Editors-in-Chief José Ricardo Figueiredo - UECE, Brazil Mário Binelli - USP, Brazil
T.M.M. Machado1 1
Department of Animal Science, Federal University of Viçosa, Viçosa, MG, Brazil; 2Postdoctoral student of FAPEMIG at this time; 3Department of Animal Science, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil.
Introduction The diffusion of embryo bisection technique would increase the offspring of genetically superior animals and maximize the efficiency of commercial programs of embryo transfer (ET) and would make viable the experiments with monozygotic twins.
Editorial Board Andrzej Bartke - Southern Illinois University, USA Antônio Carlos S. Castro - UFMG, Brazil Arlindo A. Moura - UFC, Brazil Barry D. Bavister - University of New Orleans, USA Bart Gadella - Utrecht University, The Netherlands Brian Setchell - University of Adelaide, Australia Eduardo Bustos Obregón - Universidad de Chile, Chile Eduardo Paulino da Costa - UFV, Brazil Edward L. Squires - Colorado State University, USA Fernanda da Cruz Landim-Alvarenga - UNESP, Brazil George E. Seidel Jr - Colorado State University, USA Goro Yoshizaki - Tokyo University of Marine Science and Technology, Japan Heriberto Rodriguez-Martinez, SLU, Sweden Hugo P. Godinho - PUC/Minas, UFMG, Brazil João Carlos Deschamps - UFPEL, Brazil
J.A. (Lulu) Skidmore-The Camel Reproduction Centre, UAE Katrin Hinrichs - Texas A&M University, USA Keith Betteridge - University of Guelph, Canada Margaret J. Evans - CDHB, New Zealand Martha C. Gomez - Louisiana State University, USA Parvathi K. Basrur - University of Guelph, Canada Peter J. Broadbent - UK Rex A. Hess - University of Illinois, USA Ricardo S. Calandra - IMBICE, IBYME, Argentina Richard Fayrer-Hosken - University of Georgia, USA Rüdiger W. Schulz - Utrecht University, The Netherlands Stanley P. Leibo - University of New Orleans; Audubon Research Center, USA Sue M. McDonnell - University of Pennsylvania, USA Telma M.T. Zorn – USP, Brazil William R. Allen - University of Cambridge, UK Wilma de Grava Kempinas - UNESP, Brazil
Proofreader: Keith Haag, Southern Illinois University, Carbondale, IL, USA Secretaries: Maria Helena Chaves da Silva, Eunice de Faria Lopes Animal Reproduction publishes reviews, original articles, and short communications related to the basic, applied and biotechnological aspects of animal reproductive biology. Manuscripts should be submitted online to the Editor-in-Chief. Instructions to Authors are available at http:// http://www.cbra.org.br/portal/index.htm. Animal Reproduction (ISSN 1806-9614 printed; 1984-3143 online) is published quarterly and wholly owned by the Brazilian College of Animal Reproduction (Colégio Brasileiro de Reprodução Animal - CBRA). Subscriptions and renewals are based on the calendar year. Printed versions of the journal are freely available to the members of the CBRA and electronic versions are available at the journal website. All the correspondence should be sent to the following address: Editor-in-Chief, Animal Reproduction Colégio Brasileiro de Reprodução Animal - CBRA Alameda das Princesas, 1275 - Bairro São José - 31275-180 Belo Horizonte, MG, Brazil. Phone: +55(31)3491-7122, Fax: +55(31)3491-7025, E-mail:
[email protected], Website: http://www.cbra.org.br/portal/index.htm Animal Reproduction, v.1- , n.1- , 2004Belo Horizonte, MG, Brazil: Colégio Brasileiro de Reprodução Animal, 2004Quarterly. ISSN 1806-9614 (printed); 1984-3143 (online) 1. Animal reproduction - Periodicals. I. Colégio Brasileiro de Reprodução Animal. II. Brazilian College of Animal Reproduction. CDU - 636.082.4 (81) (05) - AGRIS L10
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Material and Methods The experiment was carried out with 26 primiparous healthy Dairy Gir cows, reproductively good, body weight (BW) over 350kg, with 45-90 days postpartum, body condition score 2.0-2.5 on the scale of 1 to 5 (1), aged 36-48 months, which were distributed randomly into two treatments with (TF) and without nutritional flushing (TC). In both, the cows were submitted to the same superovulation protocol (SP). In TF the cows received energetic supplementation with 75% corn, 23% soybean meal and 2% of mineral salt, in a percentage of 1% BW, from six days before the beginning of SP to 16 days after, twice daily. Ultrasonographic evaluations were done 7 days and 1 day before the beginning of SP and at the embryo recovery day. Superovulation response was based on the corpus luteum (CL) numbers at recovery day, which was performed seven days after AI by trans-cervical technique using the Foley’s catheter. The recovered structures were washed 10 times in maintenance medium (TQC® - Holding) and classified (2, 3). Only embryos classified as compact morulae, morulae and blastocyst, and morphologically excellent were selected. Half were transferred intact (IE) to the recipients and half was submitted to bipartition (DE) and classified (4). The fresh IE and DE selected were transferred at random at the collection day to the recipients, previously synchronized for embryo transference at fixed time (FTET). Crossbreed heifers (78) were synchronized for embryo receptors (ER). The pregnancy diagnosis was made 35 days after embryo transference. The results were analysed by descriptive statistics. Results and Discussion Five embryo donors in TC and 3 in TF were discarded for some reason. The TF did not increase the CL numbers nor the collected structures. But viable embryos were 18% more frequent in TF donors than TC ones. Only 48 of 78 ER synchronized heifers showed CL of good quality for embryo transfer. Twenty four ER received intact embryos (12 from TC and 12 from TF) and 24 ER received demi-embryos (12 from TC and 12 from TF). The pregnancy rate was 20/48 of ER. The treatment did not affect the percentage of ER pregnants, but its pregnancy frequency was 10% greater in ER that received intact embryo than those that received demi-embryos. References (1) Wildman EE, Jones GM, Wagner PE, Bolman RI, Troutt Jr HF, Lesch TN. 1982. J Dairy Sci, 65(3):495-501; (2) IETS. 1999. Ed. Stringfellow, DA, Seidel, SM Translation SBTE. 180pp; (3) Linder GM, Wright Jr, RW. 1983. Theriogenology, 20(4):407-416; (4) Ribeiro STSJ, Bem AR, Silva A, Zanenga CA. 1988. Rev Centro de Ciênc Rurais, 18(S):50. Financial support: FAPEMIG (APQ 01699-08, CVZ 0334-09 and for participation in ISABR), DZO/UFV and FAZENDA CÓRREGO FRIO. E-mail:
[email protected]
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Anim. Reprod., v.9, n.4, p.984, Oct./Dec. 2012
