Feb 12, 2015 - ABSTRACT. To investigate the Antibacterial activity of Acetone extract of Gloriosa superba tuber on different human pathogens collected from ...
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
Rajesh Goud Gajula et al.
World Journal of Pharmacy and Pharmaceutical Sciences
SJIF Impact Factor 2.786
Volume 4, Issue 03, 1398-1401.
Research Article
ISSN 2278 – 4357
ANTI-BACTERIAL ACTIVITY OF GLORIOSA SUPERBA Shravya Goud Kola, Rajesh Goud Gajula*, Sathyam Raj Thurpu, Prashanth Chiliveri Department of Microbiology, Primer Biotech Research Center, Hyderabad, Telangana, India.
Article Received on 20 Jan 2015, Revised on 12 Feb 2015, Accepted on 02 Mar 2015
ABSTRACT To investigate the Antibacterial activity of Acetone extract of Gloriosa superba tuber on different human pathogens collected from ATCC, (Gram positive and Gram negative) by using agar gel well method. Antibacterial activity of all extracts from Gloriosa superba tuber were
*Correspondence for Author
checked against Gram positive bacteria such as Staphylococcus aureus
Rajesh Goud Gajula
ATCC 25923, Bacillus subtilis MTCC 441; Gram negative bacteria
Department of
such as Escherichia coli ATCC 25922, Pseudomonas aeruginosa
Microbiology, Primer
ATCC 27853 were tested. Acetone extract of Gloriosa superba tuber
Biotech Research Center, Hyderabad, Telangana, India.
showed more antagonistic effect towards the growth of pathogenic bacteria. Highest antagonistic effect showed on Escherichia coli and lowest effect on Bacillus subtilis. KEYWORDS: Gloriosa superba, Bacillus subtilis, aureus, subtilis.
INTRODUCTION Glory lily is considered as a rich source of colchicines and gloriosine(Trease et al). The flower has analgesic, antimicrobial, anti-inflammatory potential, antipoxviral potential, antithrombotic potential, larvicidal potential, enzyme inhibition potential, antitumor potential and also used in treatment of snake bite, skin disease, respiratory disorders (Alagesaboopathi, Rehana banu, Abhishek Mathur et al). Different parts of G. superba have wide variety of uses especially in traditional system of medicine. The use of traditional plants in the industrialised societies have been traced to the extraction and development of several drugs from these plants(Shrikumar et al.,2007). According to Nadkarni (1976) the root tubers are tonic, cholagogue, antiperiodic, alterative and purgative. The white flour prepared from its root tubers is given with honey in gonorrhoea and alone in leprosy, colic and intestinal worms (Dastur, 1962). All parts of the plant contain colchicines and related alkaloids and are therefore dangerously toxic if ingested, especially the tubers; contact with the leaves and stems can cause skin irritation. Various preparations of the plant are used in traditional
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medicines for a variety of complaints in both Africa and India. Therefore, in present study, attempts have been made to evaluate the Antimicrobial efficacy of the medicinal plant Gloriosa superba L. (Kamna Bhatnagar et al.). ESTIMATION OF ANTI MICROBIAL ACTIVITY Preparation of crude extract: The material collected were first thoroughly washed with water and socked in detergent to clean the surface load of microbes. These were then shade dried and coarsely powdered in mortar and pistil, and was filtered with fine muslin cloth. This fine powder of Gloriosa superba tubers (20g) were sequentially extracted with Isopropyl alcohol and Acetone at room temperature for 48 hrs. The extracts were filtered and concentrated under reduced pressure using rotary evaporator to get completely dried extracts. This was then dissolved in DMSO, dimethyl sulfoxide as a solvent. To investigate the anti bacterial activity of Acetone extract of Gloriosa superba tuber on different human pathogens collected from ATCC, (Gram positive and Gram negative) by using agar gel well method. The present study was meant to characterize pharmacological potential of different extracts from tubers of this medicinal plant. Anti bacterial activity of all extracts from Gloriosa superba tuber were checked against Gram positive bacteria such as Staphylococcus aureus ATCC 25923, Bacillus subtilis MTCC 441; Gram negative bacteria such as Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 were tested. RESULTS The leaves of Gloriosa superba were extracted with ethanol in Soxhlet assembly for 48 hrs and the percentage yield of each extract is presented in Table 1. The extracts were subjected to preliminary phytochemical analysis, the result of which revealed the antimicrobial activity in terms of zone of inhibition was shown in figures Table: 1 Anti Bacterial Activity Name of microorganism Extract Aztreonam Gloriosa superba tuber
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Bacillus subtilis
Pseudomonas aeruginosa
Escherichia coli
Staphylococcus aureus
24.5mm 26.5mm
30.5mm 29.5mm
20mm 42.5mm
20.5mm 35.5mm
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Antibacterial activity on E.coli
Antibacterial activity on B. subtilis
Antibacterial activity on P. Aeruginosa
Antibacterial activity on S. aurous
CONCLUSION The present article successfully evaluated the role of Gloriosa superba for its antibacterial activity. It was already reported that the phytochemicals have an excellent ability to act against microorganisms.This could be due to the active chemicals which are present in the Gloriosa superba making it a potential antibacterial activity. Further, upon proper clinical and pharmacological studies, Gloriosa superba could be one the suitable drugs for many diseases. REFERENCES 1. Abhishek Mathur, Satish K Verma, Santosh K Singh, Deepika Mathur, Prasad GBKS, and Dua VK, Investigation of Anti-Inflammatory Properties of Swertia Chirayta And Gloriosa Superba, Recent Research in Science and Technology, 2011; 3(3): 40- 43. 2. Alagesaboopathi, C, Antimicrobial screening of selected medicinal plants in Tamilnadu, India. Journal of Microbiology, 2011; 5(6): 617-621. 3. Dastur JF, Medicinal Plants of India and Pakistan; D.B. Taraporevala Sons and Co. Private ltd., Bombay, 1962.
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4. Kamna Bhatnagar*1 And Dr.Anirudha Rishi 2, “Antimicrobial Efficacy of An Endemic Plant Species (Gloriosa Superba Linn.)”; International Journal of Pharma and Bio Sciences, 2012. 5. Nadkarni AK, Indain Materia Medica. Popular Prakashan Pvt. LTD., Mumbai India, 1976. 6. Rehana banu, and Nagarajan N. Antibacterial Potential of Glory Lily, Gloriosa Superba Linn, International Research Journal Of Pharmacy, 2011; 2(3): 139-142. 7. Shrikumar S, Ravi TK, Approaches Towards Development and Promotion of Herbal Drugs, Phcog. Rev., 2007; 1: 180-184. 8. Trease, S.E. and Evans, D. Colchicum seed and corn. In: Pharmacognosy, 12th edn. Balliere Tindall, London, 1983; 593-59.
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