ceftazidime, cefepime, aztreonam, imipenem; aminoglycosides: gentamicin, amikacin, kanamycin, tobramycin and quinolones: ciprofloxacin, norfloxacin.
Vaginal biotic flora and its antibiotic resistance profile in Terras de Miranda Jennets Payan-Carreira R.1, Mota V.R.1, Barroso A. T. 1, Quaresma, M1, Saavedra M. J.1 1CECAV
Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal
INTRODUCTION
In this work authors report the preliminary results on the characterization of the microbiota found in the vagina of Terras de Miranda jennets, and antibiotic resistance in Gram-negative isolates. MATERIALS AND METHODS
Vaginal swab samples were obtained from the vestibule and transported in transport medium for delivery to the Microbiology Lab. This work was carried in a group of 21 jennets, aged between 4 to 20 years old and the samples were obtained in two retractions (15 animals + 6 animals). For the bacteriological analysis of the vaginal samples different and selective culture media were used, aiming a more significant bacterial growth. For the preliminary identification of the isolated ones a set of biochemical tests was carried through (oxidase, catalase, indol production, use of the citrate, metil red, vogues proskauer and lactose fermentation) Vaginal swab samples spread on plates of selective culture media
colony selection and purification
Colonies per sample were selected
isolates collection
identification
Posi-3
antibiotic profile
Antibiotic susceptibility testing was performed by agar disck-diffusion method (by CLSI recommendations), including 18 antibiotics. β-lactams: amoxicillin, amoxicillin/clavulanic acid, ticarcillin, ticarcillin/clavulanic acid, piperacillin, piperacillin/tazobactam, cephalotin, cefotaxime, ceftazidime, cefepime, aztreonam, imipenem; aminoglycosides: gentamicin, amikacin, kanamycin, tobramycin and quinolones: ciprofloxacin, norfloxacin.
RESULTS and DISCUSSION
From the vaginal samples, bacteria’s pertaining to the different generic groups were found: Grampositive bacteria, such as Staphylococcus spp. and Enterococcus spp.; and Gram-negative bacteria, such as Enterobactereaceae (Escherichia coli, Klebsiella and Enterobacter) and not Enterobactereaceae (Pseudomonas spp.). Antibiotic Resistance Profile Regarding to the resistance profile to antibiotics, the most frequently observed resistance was to amoxicillin and ticarcillin (penicillins), even when associated to the beta-lactamases inhibitor (acid clavulanic), as well as the cephalosporin of st 1 generation studied (cephalotin). Resistance to aztreonam (antibiotic beta-lactam of the group of the monobactams) was also observed in some cases. Future studies should be focused to characterization of antibiotic-resistance genes.
Amoxicillin Amoxicillin-Clavulanic Acid Ticarcillin Ticarcillin/Clavulanic Acid Piperacilina Piperacillin/Tazobactam Imipenem Cefalotin Cefotaxime Ceftazidime Cefepime Aztreonam Ciprofloxacin Norfloxacin Amikacin Kanamycin Tobramycin Gentamicin
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16 th Internacional Congress Animal Reproduction, Budapest, 2008
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