Antimicrobial Activity of Metal & Metal Oxide Nanoparticles Interfaced With Ligand Complexes Of 8Hydroxyquinoline And Amino Acids Gaurav Bhanjana, Neeraj Kumar, Rajesh Thakur, Neeraj Dilbaghi, and Sandeep Kumar Citation: AIP Conf. Proc. 1393, 173 (2011); doi: 10.1063/1.3653665 View online: http://dx.doi.org/10.1063/1.3653665 View Table of Contents: http://proceedings.aip.org/dbt/dbt.jsp?KEY=APCPCS&Volume=1393&Issue=1 Published by the American Institute of Physics.
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Antimicrobial Activity of Metal & Metal Oxide Nanoparticles Interfaced With Ligand Complexes Of 8Hydroxyquinoline And α-Amino Acids Gaurav Bhanjana, Neeraj Kumar, Rajesh Thakur, Neeraj Dilbaghi and Sandeep Kumar Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar Haryana. 125001
[email protected] Abstract. Antimicrobial nanotechnology is a recent addition to the fight against disease causing organisms, replacing heavy metals and toxins. In the present work, mixed ligand complexes of metals like zinc, silver etc. and metal oxide have been synthesized using 8-hydroxyquinoline (HQ) as a primary ligand and N-and/O-donor amino acids such as Lserine, L-alanine, glycine, cysteine and histidine as secondary ligands. These complexes were characterized using different spectroscopic techniques. The complexes were tested for antifungal and antibacterial activity by using agar well diffusion bioassay. Keywords: M etal nanoparticles, M etal oxide nanoparticles, 8-Hydroxyquinoline, Glycine, L-Alanine. PACS : 87.10vg, 87.19xb, 87.19xg
The complexes prepared from 8-hydroxyquinoline, amino acids and Transition metal ions could present interesting metallo-organic compounds with significant biological activity. Further, no work has been carried out on many transition metal ion complexes with 8-hydroxyquinoline and amino acids as ligands. It was, therefore, considered worthwhile to study the complexation and to determine the biological activity of these new complexes Mixed ligand transition metal ions complexes were prepared with 8-hydroxyquinoline as a primary ligand and amino acids such as L-alanine/L-serine/glycine as a secondary ligand (1). Transition metals were used due to its high co-ordination number and ability to from stable complexes. These complexes were characterized on the basis of elemental analysis, conductance measurement, spectral and thermal studies and screened for their antibacterial, antifungal and cytotoxic properties (2, 3).
INTRODUCTION Coordination chemistry stands as landmark in the area of scientific advancement embracing most diverse branches of science, engineering and technology. In the rapidly developing field of hydrometallurgy, the metal complexes have large utility in the metallurgical operations, great practical importance in dying and textile industries, in analytical chemistry, in engineering technology, and also have important functional values in biological, biomedical and industrial transformations. Coordination compounds, generally known as inorganic complexes, are of great practical importance in industries and also have important functional values in nature. It is well known that mixed ligand complexes of some metals play an important role in the activation of enzymes and are used for storage as well as for transport of active materials. Mixed ligand complexes are established to be biologically active against pathogenic microorganisms, further, metal complexes, which include 8-hydroxyquinoline as primary ligand exerts biological activity. Amino acids form complexes with metal atoms and exhibit significant biological and enzymatic activities.
PREPARATION OF MEDIA 5 g of Luria Broth (HIMEDIA) was dissolved in 250 mL of water in a 500 mL flask by swirling. 2 % Agar powder (SRL) was taken & was added in this
International Conference on Advances in Condensed and Nano Materials (ICACNM-2011) AIP Conf. Proc. 1393, 173-174 (2011); doi: 10.1063/1.3653665 © 2011 American Institute of Physics 978-0-7354-0963-7/$30.00
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mmol) of aqueous solution of amino acid was added to this hot solution with constant stirring. The complexes were obtained by raising the pH (from 2.8 to 6.5 if required) of the reaction mixtures by adding dilute ammonia solution. The mixtures were cooled, filtered and washed with water, followed by ethanol. The solid complexes thus prepared were dried under vacuum. The binding was confirmed by FTIR. Thereafter, antibacterial study was done in a similar way as that for metal oxide nanoparticles.
flask. The flask was heated in the oven / hot plate to dissolve the agar. Further the flask was plugged with cotton (non adsorbent) and the plug was wrapped with aluminium foil .These flasks were then sterilized in the autoclave (moist heat sterilization) at 121.6 O C temp. and 15 lbs (Psi) or 2 atm. pressure for 15 minutes .Also the other required items, like, cork borer, tips of micropipette, distilled water in a bottle, were sterilized along with these.
Transmittance (a.u.)
Antibacterial Study Of Metal Oxide Nanoparticles The (LBA) ‘Luria broth agar’ medium was melted in an oven and allowed to cool to room temp. The medium was poured in disposable petri plates (previously sterilized) in molten state and allowed to solidify. The bacteria culture from the fresh culture was taken (100µl) & was spread over the media with the help of test tube. Then the wells (atleast 5 mm) were punched in the medium with the help of sterilised cork borer. The wells were labeled with the help of marker on the back side of petri plates. Then different concentrations of metal oxide nanoparticles suspension were poured in the appropriate wells according to the labeling, with the help of micropipette. The petriplates were closed and sealed with parafilm and were incubated in the incubator (REMI) for 24 hours. After 24 hrs, the zones of inhibition were observed and the diameters of zones were measured. .
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FIGURE 2. FTIR spectra of Zr(IV) mixed ligand complex (using glycine as a secondary ligand)
FIGURE 3. Particles size analyzer results of ZnO nanoparticles and mixed ligand complex.
The X axis and Y axis correspond to particle size (diam., nm) and intensity (%) respectively.
ACKNOWLEDGMENTS FIGURE 1. The zone of inhibition for zinc oxide nanoparticles and aluminium oxide nanoparticles
We are grateful to the Chairperson, Dept of Bio & Nanotechnology, GJUS&T, Hisar for providing us research facilities.
Preparation Of Mixed Ligand Complexes
REFERENCES
Mixed ligand metal oxide complexes were prepared from metal oxide nanoparticles using 8hydroxyquinoline (sd fine chem. Limited) as a primary ligand and amino acids like L-serine or glycine as a secondary ligand. 230 mg of nanoparticles were dissolved in 10 mL of distilled water. 10 mL ethanolic solution of 8-hydroxyquinoline (290 mg) was added to this aqueous solution. The mixture was stirred and kept in a boiling water bath for 10 min. 10 mL (1
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