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Accepted 2 December 2009 Revised 2 September 2009 Received 5 March 2008 Indian J. Pharm. Sci., 2009, 71 (6): 691-695
Antioxidant and Antidiabetic Activity of Helicteres isora (L.) Fruits M. SUTHAR*, G. S. RATHORE AND A. PAREEK1 L. B. S. College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302 004, 1L. M. College of Science and Technology (Pharmacy Wing), Sector-A, Shastri Nagar, Jodhpur-342 003, India
Suthar et al.: Antioxidant and Antidiabetic Activity of Helicteres isora The present investigations evaluated the antioxidant and antidiabetic activity of Helicteres isora (L.) fruits belonging to the family Sterculiaceae. The hot water extract of Helicteres isora fruits was prepared and screened for its in vitro antioxidant activity using 1,1-diphenyl,2-picryl hydrazyl assay, ß-carotene-linoleate model and microsomal lipid peroxidation or thiobarbituric acid reactive species assays and the IC50 values were calculated. Antidiabetic effect was studied using the in vitro glucose uptake in the isolated rat hemi-diaphragm model. The hot water extract of Helicteres isora showed maximum activity with IC50 value 25.12±0.18 µg/ml for 1,1-diphenyl,2-picryl hydrazyl assay method, and low activity with IC50 value 740.64±4.76 µg/ml for microsomal lipid peroxidation assay. In the ß-carotene-linoleate model, the extract showed 45.63% antioxidant activity. The extract produce a significant (P
