Supplemental Figure RT-PCR analysis of SMN2 transcripts from SMA fibroblasts following initial screening with exon 8 targeting 2´O-Methyl AOs (300, 150, ...
OMTN, Volume 11
Supplemental Information
Antisense Oligonucleotide-Mediated Terminal Intron Retention of the SMN2 Transcript Loren L. Flynn, Chalermchai Mitrpant, Ianthe L. Pitout, Sue Fletcher, and Steve D. Wilton
Supplemental Information
Supplemental Figure RT-PCR analysis of SMN2 transcripts from SMA fibroblasts following initial screening with exon 8 targeting 2´O-Methyl AOs (300, 150, 75 nM). Anti ISS-N1 was used as a positive control for transfection efficiency and a sham control AO was used as a transfection negative control. A 100 bp size marker was used for comparison, and a RT-PCR no-template negative control was loaded in the final lane.
Supplemental Methods Sequences and details of all primers used in this study. Primer name
