Apoptosis assay (TUNEL staining) Apoptosis was ...

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Apoptosis was determined using the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay (DeadEnd Colorimetric TUNEL ...
Apoptosis assay (TUNEL staining) Apoptosis was determined using the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay (DeadEnd Colorimetric TUNEL System, Promega, Madison, WI), following the manufacturer’s instructions. Cells were transfected with as described above. After 72 hours, cells were fixed, treated with proteinase K followed by the incubation with the TdT reaction mixture. Cells were then incubated in streptavidin-HRP, and the color was developed with diaminobenzidine as a chromogen. Apoptotic nuclei exhibiting fragmented DNA were stained dark brown and the number of apoptotic cells was expressed as a percentage of the total population in four separate 200× fields. Cells exposed to RQ1 DNase solution (Promega, Madison, Wisconsin) served as a positive control.

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