Applied Microbiology and Biotechnology Supplementary Material
Biochemical and proteomic characterization of the extracellular enzymatic preparate of Exiguobacterium undae, suitable for efficient animal glue removal
Lenka Jeszeová1, Vladena Bauerová-Hlinková1*, Peter Baráth2, Andrea Puškárová1, Mária Bučková1, Lucia Kraková1, Domenico Pangallo1*
1
Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia
2
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia
*Corresponding authors:
[email protected];
[email protected]
Fig. S1 Degradation of rabbit skin and bone animal glue on a sandstone surface. A. Drops of rabbit skin and bone animal glues spotted on the surface. B. Treatment with the E. undae biopreparate at room temperature. C. Dried stone surface after the removal of the dissolved drops of rabbit skin rabbit and bone animal glues.
Fig. S2 Degradation of rabbit skin animal glue on a glass surface. A. Drops of rabbit skin animal glue spotted onto the glass surface of a Petri dish. B. A degraded glue drop after treatment with the E. undae biopreparate for ≈ 30 min at 37°C. C. A non-degraded animal glue drop after treatment with distilled water in the same conditions.
Fig. S3 Degradation of rabbit skin and bone animal glues on a Fagus sylvatica wood surface. A. Drops of rabbit skin and bone animal glues spotted on the wood surface. B. Treatment with the E. undae biopreparate at room temperature. C. Dried wood surface after the removal of the dissolved glue drops. Treated drops are in black circles.
Fig. S4 Degradation of rabbit skin and bone animal glues on a Picea abies wood surface. A. Drops of rabbit skin and bone animal glues spotted on the wood surface. B. Treatment with the E. undae biopreparate at room temperature. C. Dried wood surface after the removal of the dissolved glue drops. Treated drops are in black circles.
Fig. S5 Degradation of rabbit skin and bone animal glues on a Quercus sp. wood surface. A. Drops of rabbit skin and bone animal glues spotted on the wood surface. B. Treatment with the E. undae biopreparate at room temperature. C. Dried wood surface after the removal of the dissolved glue drops. Treated drops are in black circles.
