#8454) and psPAX2 (addgene, #12260), in HEK293 FT cells. The generated lentivirus encoding Becn1 or. Becn1F121A were used to infect MIN6 cells at the ...
Cell Reports, Volume 23
Supplemental Information
Autophagy Differentially Regulates Insulin Production and Insulin Sensitivity Soh Yamamoto, Kenta Kuramoto, Nan Wang, Xiaolei Situ, Medha Priyadarshini, Weiran Zhang, Jose Cordoba-Chacon, Brian T. Layden, and Congcong He
24-h fasting Pancreas
3
4
1
2
3
4
LC3-I LC3-II
1.6 1.2
*
0.8
p62
0.4
actin
0
WT
F121A
1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0
*
WT
Liver
p62 actin
3
4
1
2
3
4
0.8
*
0.6 0.4 0.2 0
WT
F121A
1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0
NS
WT
F121A
1.6 1.2 0.8 0.4 0
F121A
LC3-II:LC3-I
LC3-I LC3-II
2
LC3-II:actin
Mouse#: 1
1.0
F121A
p62:actin
WT
NS
2.0
LC3-II:LC3-I
2
LC3-II:actin
Mouse#: 1
2.0
F121A
p62:actin
WT
1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0
WT
F121A
NS
WT
F121A
Figure S1. Autophagy markers under the starvation condition in Becn1F121A mice compared to WT mice. Related to Figure 1. Western blot analysis (left) and quantification (right) of autophagy markers p62, LC3-I and LC3-II in pancreas (upper) and liver (lower) of WT and Becn1F121A mice after 24-h fasting. One-way ANOVA. N=4. *, P
