Biodegradation of Lambda-cyhalothrin pesticide by Serratia

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Biodegradation of Lambda-cyhalothrin pesticide by Serratia marcescens S1A isolated from cotton crop soil. Waheed Ahmed Ghumro, Muneer Ahmed Qazi and ...
Research Area: Environmental Microbiology / Soil & Agricultural Microbiology

Biodegradation of Lambda-cyhalothrin pesticide by Serratia marcescens S1A isolated from cotton crop soil

Waheed Ahmed Ghumro, Muneer Ahmed Qazi and Nisar Ahmed Kanhar* Department of Microbiology, Shah Abdul Latif University Khairpur (Mir’s), Sindh Pakistan Corresponding author’s Email: [[email protected]]

Abstract Lambda-Cyhalothrin, a synthetic pyrethroid, is used as pesticide to control a wide range of pests in a variety of agricultural applications. Although, it is considered as potentially harmful environmental pollutant and poses serious health hazard to other organisms, very limited research has been carried out for possible environment friendly removal of the pollutant. Interestingly, soil microorganisms have been known to possess an impressive genetic makeup that helps them to perform crucial job to eliminate such harmful pollutants from the environment by various means including biodegradation. In present study, the bacterial strain of Serratia marcescens S1A capable of degrading Pyrethroid Lambda-cyhalothrin pesticide was successfully isolated from cotton crop soil and taxonomically characterized based on its cellular morphology, physiological and chemotaxonomic characteristics as well as phylogenetic similarity index of 16S rRNA gene sequence. The bacterium was found to exhibit 100% degradation of Lambdacyhalothrin (conc. 250 mg L-1, w/v) during 20 days of incubation at 30°C under laboratory conditions. The percent removal of Lambda-cyhalothrin was estimated using UV-Vis Spectroscopy at 287 nm (λ max for Lambda-cyhalothrin) against the standard curve prepared for known concentrations of Lambda-cyhalothrin. The bacterial isolate of present study has substantial potential for various environmental applications requiring cleanup of the pesticide polluted sites.

Keywords: Biodegradation; cotton crop; Lambda-cyhalothrin; Serratia marcescens