Dec 21, 1989 - LALAN. 0. L. S. V. L. V. S. A R C. L C a V. LA. 0. 0GC. C. AC010_ 1CACrr 001IG1 1V0GMt_ 1cOG0tA1 OGAm as. I ;Os0C cs10c aC0. 2,00. CaQ.
674 Nucleic Acids Research, Vol. 18, No. 3
The complete Bombyx mori
sCa~ ~aT (C)Oxford
of
sequence
University Press 1990
retrotransposon in
mag, a new
Jean-Jacques Michaille, S. Mathavan, Janine Gaillard and Annie Garel Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 106, Universite Lyon 1, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne cedex, France EMBL accession no. X17219
Submitted December 21, 1989
unusually short terminal repeat, different from the arrangement of the LTR of retroviruses, has been confirmed by sequencing other copies of this element selected from a Bombyx genomic library. From the phylogenetic tree established on the RT sequence (2 and personal communication), this retrotransposon can be positioned among the copia like family elements of
Mag, a 4564bp long transposable element has been discovered in the large intron of a cloned allele of the Ser2 gene (1). A few copies (7 tol4) are dispersed into the genome of different strains of Bombyx mori. It is flanked by a Sbp repeat of the target sequence and is bordered by direct terminal repeats of 77 nucleotides. Two large open reading frames are organized as the gag and pol genes of retroviruses in the non coding strand of the Ser2 gene. The ORFI is 258 codons long and presents the characteristic features of two nucleic acid binding motifs (underlined a). The ORF2 (1195 codons) shows strong homologies with the retroviral protease (b), reverse transcriptase (c), Rnase H (d) and endonuclease (e, f), in this order. The
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Michaille,J.J., Garel,A. and Prudhomme,J.C. (1990) Gene in press. Xiong,X. and Eickbush,Th.H. (1988) Mol. Biol. Evol. 5, 675-690.
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REFERENCES
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