As the Fab part of primary antibodies are involved with antigen recognition, the Fc portion of the antibody is required to be recognized by the secondary antibody ...
Name of the Candidate: Manish Kumar Subject: Life Sciences Roll No: 314299 Booklet Code (A/B/C): B Representation/Grievance against Question No.: 23
CSIR Answer Key: (3)
_____________________________________________________________________________________ Justification: As the Fab part of primary antibodies are involved with antigen recognition, the Fc portion of the antibody is required to be recognized by the secondary antibody in the immunodetection procedures. In this way secondary antibodies can have broader epitope recognition than the antigen specific primary antibodies and the anti-allotypic antibodies serve the purpose well here.
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Immunology For Life Scientists (2/E, Page No. 44-45) By Lesley-Jane Eales ( John Wiley & Sons Ltd.) Kuby Immunology (6/E, Page No. 100-102) By Kindt/Goldsby/Osborne ( W. H. Freeman and Company, New York) Herzenberg LA, Jones PP, Herzenberg LA(1974) Mouse immunoglobulin allotypes: characterization and use in cellular immunology. Ann Immunol (Paris) Jan;125C(12):71-83 Roy Jefferis & Marie-Paule Lefranc (2009) Human immunoglobulin allotypes, mAbs, 1:4, 332-338, DOI: 10.4161/mabs.1.4.9122
