Cause of Death and Sudden Cardiac Death After ... - Semantic Scholar

2 downloads 12 Views 83KB Size Report
pulmonary hypertensive arteriopathy based on the grading scheme of Heath and ... hypereosinophilia, a polymorphous inflammatory infiltrate, myophagocytosis, and .... cancer in a patient who died 15 days after transplantation was clearly ..... rosis, and all were papillary neoplasms of low nuclear grade. The lesions called ...

Anatomic Pathology / DEATH AFTER CARDIAC TRANSPLANTATION

Cause of Death and Sudden Cardiac Death After Heart Transplantation An Autopsy Study Russell T. Alexander, MD, and Charles Steenbergen, MD, PhD Key Words: Autopsy; Cardiac transplantation; Rejection; Coronary atherosclerosis; Fibrointimal hyperplasia DOI: 10.1309/713JVR58EF916DN5

Abstract Postmortem findings in 39 patients following cardiac transplantation are presented. Causes of death were right-sided heart failure after transplantation (6 [15%]), infection (5 [13%]), multisystem organ failure (4 [10%]), complications of noncardiac surgery (3 [8%]), acute rejection (3 [8%]), malignant neoplasm (3 [8%]), graft vascular disease (3 [8%]), preservation procurement injury (3 [8%]), cardiac arrhythmia (2 [5%]), other (4 [10%]), and unclear (3 [8%]). Seven patients in medically stable condition died after a sudden cardiac arrest, and these constituted 27% (7/26) of deaths more than 1 month after transplant. The 7 sudden cardiac arrests were due to graft vascular disease (2 [29%]), acute rejection (2 [29%]), cardiac arrhythmias (2 [29%]), and hyperkalemia during an exacerbation of acute renal failure (1 [14%]). In 3 of the 5 patients who died of sudden cardiac arrest not due to acute rejection, hemodialysis and plasmapheresis were triggers of the event. Pulmonary hypertensive arteriopathy was associated with early death and rightsided heart failure, and 6 of 8 patients with these changes died perioperatively or postoperatively.

740 740

Am J Clin Pathol 2003;119:740-748 DOI: 10.1309/713JVR58EF916DN5

Orthotopic heart transplantation is a well-established procedure to prolong the life of patients with severe cardiomyopathy due to a variety of causes. More than 57,000 heart transplants had been reported to the Registry of the International Society for Heart and Lung Transplantation through the year 2000.1 Although substantial data have been reported as to the cause of death in these patients,1-3 most autopsy-based studies are at least a decade old.4-7 Few new autopsy data have been presented since McManus8 highlighted the pressing need for these types of studies to better understand allograft pathology, transplant arteriopathy, and the spectrum of disease present in heart transplant recipients. We reviewed the autopsy reports and clinical records of cardiac transplant recipients during a 16year interval to determine the cause of death. A subgroup of patients who died of sudden cardiac arrest is discussed in detail.

Materials and Methods From 1985 to 2001, 325 orthotopic heart transplants were performed at Duke University Medical Center (DUMC), Durham, NC. Of the 325 patients, 97 died and 39 were autopsied, for an autopsy rate of 40%. Only patients who had received their first heart transplant were included in the study. Two of the autopsied patients underwent cardiac transplantation at other medical institutions but were receiving care at DUMC when they died. The types of autopsies completed were full in 25 cases (64%), limited to the heart and lungs in 5 (13%), heart only in 5 (13%), chest and abdomen only in 3 (8%), and abdomen only in 1 (3%). © American Society for Clinical Pathology

Anatomic Pathology / ORIGINAL ARTICLE

The cause of death was determined by a review of the clinical and autopsy records. Only the primary event that led directly to death or resulted in clinical deterioration of the patient’s condition culminating in death was deemed the cause of death. The cause of death was stratified based on survival time: 0 to 1 month, 1 month to 2 years, and greater than 2 years. A subgroup of patients who were in medically stable condition and then died after a sudden cardiac arrest are discussed in detail by correlating their clinical history and postmortem cardiac pathology. Severely ill hospitalized patients who died of a cardiac arrest are not included in this discussion. The amount of coronary artery disease was determined by a gross estimation of the most severe reduction in crosssectional area within a coronary artery or major branch thereof. We subdivided coronary disease into mild (50%74% stenosis), moderate (75%-90% stenosis), and severe (>90% stenosis). Coronary artery disease in the cardiac allograft can be divided into 2 types: the focal eccentric atherosclerotic plaques seen in the general population (hereafter referred to as atherosclerosis) and the diffuse concentric fibrointimal thickening (hereafter referred to as fibrointimal hyperplasia) that is characteristic of transplanted hearts and is thought to represent chronic rejection.9-11 Although these 2 disease processes are not mutually exclusive,10 we assigned each patient to 1 category based on the predominant disease process present.

Results Patient Population The 39 autopsied patients were 29 men and 10 women; 26 were white, 12 were black, and 1 was Native American. Six pediatric patients ranged in age at transplantation from 18 days to 15 years (mean, 9.2 years). Pediatric survival ranged from 0 days to 3.2 years (mean, 339 days). The adults’ age range at transplantation was 30 to 61 years (mean, 48.1 years). Adult survival ranged from 0 days to 9.8 years (mean, 2.6 years). The indications for transplantation in adult, pediatric, and all patients are summarized in ❚Table 1❚. These numbers are similar to those reported elsewhere.1,3,5,6 Sudden Cardiac Arrest A subgroup of 7 patients who were in medically stable condition died after a sudden cardiac arrest ❚Table 2❚. Two deaths were attributed to graft vascular disease (GVD). Both patients had severe coronary artery fibrointimal hyperplasia. They lived 3.2 and 6.3 years, and both had evidence of myocardial ischemia in the form of an acute myocardial infarction or extensive, wavy fiber change. Two other deaths © American Society for Clinical Pathology

❚Table 1❚ Reason for Cardiac Transplantation*

Ischemic disease Idiopathic disease Congenital disease Viral myocarditis Amyloid changes Valvular disease Eosinophilic or restrictive changes *

All Cases (n = 39)

Pediatric Cases (n = 6)

Adult Cases (n = 33)

19 (49) 12 (31) 3 (8) 2 (5) 1 (3) 1 (3) 1 (3)

0 (0) 0 (0) 3 (50) 2 (33) 0 (0) 0 (0) 1 (17)

19 (58) 12 (36) 0 (0) 0 (0) 1 (3) 1 (3) 0 (0)

Data are given as number (percentage).

were attributed solely to cardiac arrhythmias only after no other clinical or anatomic cause could be found to explain the death; complete autopsies were performed on both. They had mild atherosclerosis. All of the aforementioned 4 patients had cardiac hypertrophy and/or dilation, which is an independent risk factor for arrhythmia and sudden death.12,13 Another patient died due to an arrhythmia during an exacerbation of acute renal failure. The last 2 deaths were due to acute cellular allograft rejection. Two other patients died suddenly, but whether this was due to sudden cardiac arrest is unclear. One had a complex medical history that included Staphylococcus epidermidis sepsis and acute and chronic cerebrovascular accidents. Autopsy revealed mild coronary artery atherosclerosis and right and left ventricular hypertrophy (heart weight, 624 g). The second patient had a history of medical noncompliance and drug abuse and was admitted after 3 days of dyspnea and chest pain. Her initial admission drug screen was positive for cocaine, but a repeated screen was negative. She died suddenly soon after admission. Her cardiac pathology included mild coronary artery atherosclerosis and focal acute contraction band necrosis that was thought to be consistent with either the toxic effects of cocaine or aggressive resuscitation. Cause of Death The cause of death varied with increasing time from transplantation ❚Table 3❚. Right-sided heart failure, multisystem organ failure (MSOF), and preservation procurement injury are included in Table 3 as complications of transplantation because these deaths occurred during the immediate postoperative period and were related directly to transplantation. Early Deaths After Transplantation Fourteen patients died perioperatively or postoperatively without ever reaching a medically stable condition ❚Table 4❚. Thirteen died during the first month after transplantation, while 1 survived for 50 days. The causes of death in these 14 Am J Clin Pathol 2003;119:740-748 741 DOI: 10.1309/713JVR58EF916DN5

741 741

Alexander and Steenbergen / DEATH AFTER CARDIAC TRANSPLANTATION

❚Table 2❚ Sudden Cardiac Arrest Case Survival After No. Transplant 1

41 d

Type of Coronary Artery Disease/Maximum Percentage Stenosis Atherosclerosis/50-74

2

2.7 y

—/90

4

6.3 y

Fibrointimal hyperplasia/>90

5

7.2 y

Atherosclerosis/50-74

6 7

1.9 y 3.1 y

Fibrointimal hyperplasia/50-74 —/2 y 0 (0) 1 (6) 0 (0) 3 (18) 1 (6) 3 (18) 3 (18) 0 (0) 1 (6) 3 (18) 2 (12) 17 (44)

Data are given as number (percentage).

742 742

Am J Clin Pathol 2003;119:740-748 DOI: 10.1309/713JVR58EF916DN5

© American Society for Clinical Pathology

Anatomic Pathology / ORIGINAL ARTICLE

❚Table 4❚ Perioperative and Postoperative Deaths Case No.

Age (y)

Transplant Year

Survival (d)

1 2 3 4 5 6 7 8 9 10 11 12 13 14

45 41 38 12 55 0.25 57 0.06 41 46 58 51 54 55

1986 1986 1988 1988 1989 1990 1990 1994 1995 1995 1995 1999 2000 2000

0 0 3 1 0 2 15 9 6 0 15 8 50 10

*

Pulmonary Hypertensive Arteriopathy*

Cause of Death Right-sided heart failure Right-sided heart failure Right-sided heart failure Right-sided heart failure Preservation procurement injury Right-sided heart failure Infection Preservation procurement injury Right-sided heart failure Preservation procurement injury Multisystem organ failure Multisystem organ failure Multisystem organ failure Multisystem organ failure

II II 0 III Not evaluated Not evaluated 0 0 II Not evaluated 0 II I 0

0 indicates no evidence of pulmonary hypertensive arteriopathy; I, II, and III are Heath and Edwards grades of pulmonary hypertensive arteriopathy14; cases 5, 6, and 10 were not evaluated because the autopsy was limited to the heart only.

gram-positive filamentous organisms consistent with Nocardia species. One patient who survived 150 days after transplantation became critically ill after developing cytomegalovirus (CMV) colitis. The autopsy revealed CMV pneumonia and gastroenteritis with a concomitant infection of the lungs, brain, and skin by Scedosporium apiospermum. Another patient died 1.1 years after transplantation, after a lung infection due to Aspergillus species. The final patient died following CMV gastroenteritis that occurred 5.0 years after transplantation. His course was complicated by a central nervous system infection with human herpesvirus 6. Complications of Noncardiac Surgery Three patients died of complications of noncardiac surgery. The first died 3.2 years after transplantation when an anal papilloma excision was complicated by sepsis due to Pseudomonas and Candida organisms. One month after a radical prostatectomy for prostate cancer, the second patient experienced wound dehiscence complicated by necrotizing fasciitis and peritonitis. He died 4.0 years after transplantation. The last patient underwent a right-sided upper lobectomy for squamous cell carcinoma of the lung, which was complicated by a polymicrobial pneumonia and sepsis due to P aeruginosa and Candida albicans. He died 9.8 years after transplantation. Acute Rejection Acute cellular rejection is characterized by an inflammatory infiltrate that may be associated with damage to the heart. The International Society for Heart and Lung Transplantation assigns grades of 0 to 4, depending on the nature of the infiltrate and the presence of myocardial and vascular damage. 15 Acute rejection was the cause of death in 3 patients. Two of them, aged 17 and 46 years, had sudden © American Society for Clinical Pathology

cardiac arrests and died 1.9 and 3.1 years after transplantation, respectively (cases 6 and 7, Table 2). Both had grade 3B rejection at postmortem examination. The third patient was a 46-year-old man who sought care 2 years after transplantation because of malaise, shortness of breath, and chest pressure. During evaluation, he had a cardiac arrest. The autopsy revealed grade 3B rejection in the heart. Malignant Neoplasms Malignant neoplasms were the clear cause of death in 2 patients and the presumed cause in another ❚Table 5❚. One patient was a 62-year-old man in whom fever, chills, and a pleural effusion developed 2.4 years after transplantation. When he died, Kaposi sarcoma involved his lung, liver, and skin. A second patient was a 43-year-old man in whom ascites, fever, shortness of breath, and renal failure developed 7.1 years after transplantation. He was diagnosed with diffuse large B-cell lymphoma. MSOF quickly developed, and when he died, lymphoma involved his mediastinal and abdominal lymph nodes, colon, omentum, pleura, small intestinal serosa, and splenic capsule. The third patient was a 49-year-old man who was being evaluated for pheochromocytoma. He had abdominal pain and hypertension 2.9 years after transplantation and then died after a cardiac arrest. The autopsy revealed an enlarged, mostly necrotic adrenal with medullary expansion, which was suggestive of pheochromocytoma. The postmortem heart exhibited rare foci of acute and chronic inflammation, as well as diffuse contraction band necrosis and wavy fiber change in all coronary territories of the left ventricle. These changes are consistent with catecholamine toxicity, as might be expected with a pheochromocytoma.16 Seven additional patients had posttransplant malignant neoplasms; these often were multiple (Table 5). Skin cancers were common and found in 3 patients. A case of colon Am J Clin Pathol 2003;119:740-748 743 DOI: 10.1309/713JVR58EF916DN5

743 743

Alexander and Steenbergen / DEATH AFTER CARDIAC TRANSPLANTATION

❚Table 5❚ Malignant Neoplasms Occurring After Transplantation Case No. 1 2 3 4 5 6 7 8 9 10

Malignant Neoplasm Colonic adenocarcinoma Recurrent DFSP Renal adenoma Kaposi sarcoma Pheochromocytoma Prostate carcinoma Renal adenoma Skin, SCC Skin, BCC Lung, PTLD Skin, SCC DLBL Renal adenoma Lung, SCC Hodgkin lymphoma Renal cell carcinoma, bilateral Skin, SCC (2 occurrences) Skin, SCC (2 occurrences) Lung, SCC Renal cell carcinoma, bilateral

TransplantUnexpected Neoplasm Diagnosis Interval Found at Autopsy 15 d 1.1 y 1.8 y 2.4 y 2.9 y 3.6 y 4.0 y 1.8 y 1.8 y 4.6 y 5.0 y 7.1 y 7.1 y 5.4 y 7.2 y 9.6 y 7.6 y 9.0 y 9.7 y 9.8 y

Yes No Yes No No No Yes No No No No No Yes No Yes Yes No No No Yes

Cause of Death Unclear Pulmonary embolus

Survival 15 d 1.8 y

Kaposi sarcoma Pheochromocytoma Complications of noncardiac surgery

2.4 y 2.9 y 4.0 y

Infection (GI CMV)

5.0 y

DLBL

7.1 y

Unclear

7.2 y

Cirrhosis Complications of noncardiac surgery

9.6 y 9.8 y

BCC, basal cell carcinoma; CMV, cytomegalovirus; DFSP, dermatofibrosarcoma protuberans; DLBL, diffuse large B-cell lymphoma; GI, gastrointestinal; PTLD, posttransplant lymphoproliferative disorder; SCC, squamous cell carcinoma.

cancer in a patient who died 15 days after transplantation was clearly incidental to the transplantation history. A patient who died of a pulmonary embolus had a recurrence of dermatofibrosarcoma protuberans (DFSP) that was diagnosed 26 years before cardiac transplantation. Two patients died of complications following surgical resection of their tumors: one had prostate adenocarcinoma and the other had squamous cell carcinoma of the lung. A patient in whom posttransplant lymphoproliferative disorder developed 4.6 years after transplantation later died of complications following a gastrointestinal CMV infection. An unexpected nodular sclerosis type Hodgkin lymphoma involving mediastinal lymph nodes was discovered at autopsy in a patient who had a sudden cardiac arrest (case 5, Table 2). This same patient had a lung squamous cell carcinoma resected 5.4 years after transplantation. Renal adenomas and carcinomas were a necropsy finding in 5 patients and were found in 17% (5/29) of autopsies that included examination of the abdomen. Graft Vascular Disease GVD was the cause of death in 3 patients. All had severe fibrointimal hyperplasia. One was a girl who was 13 years old at the time of transplantation and who survived 3.2 years before dying of a sudden cardiac arrest (case 3, Table 2). Her heart had diffuse, wavy fiber change and slight interstitial and perivascular fibrosis. A 51-year-old woman who survived 6.3 years after transplantation also died of a sudden cardiac arrest (case 4, Table 2). Multiple small acute infarcts were seen in the posterior and anteroseptal portions of the left ventricle; 744 744

Am J Clin Pathol 2003;119:740-748 DOI: 10.1309/713JVR58EF916DN5

diffuse interstitial and perivascular fibrosis also were present. The last patient to die of GVD was a man who was 55 years old at the time of transplantation. He died 3.3 years after transplantation; he sought care because of 3 days of dyspnea and a syncopal episode. The autopsy revealed multiple acute, organizing, and old infarcts in the left ventricle. Other Causes of Death The causes of death in 4 patients are listed as other (Table 3). A 51-year-old man died 1.8 years after transplantation as a result of complications of a pulmonary embolus. A 53-year-old man with a medical history of hepatitis B and cirrhosis was admitted to the hospital 9.6 years after transplantation because of hepatic failure. Hepatitis B infection in heart transplant recipients is associated with active viral replication and an aggressive clinical course.17 He died due to hepatic encephalopathy and renal failure. A 61-year-old man had a massive hemorrhagic infarct in the left middle cerebral artery territory 2.7 years after transplantation. The fourth patient had a sudden cardiac arrest secondary to hyperkalemia and acute renal failure (case 2, Table 2).

Discussion The causes of death in this study were complications of transplantation (13 [33%]), infection (5 [13%]), complications of noncardiac surgery (3 [8%]), acute rejection (3 [8%]), malignant neoplasm (3 [8%]), GVD (3 [8%]), cardiac arrhythmia (2 [5%]), other (4 [10%]), and unclear (3 [8%]). © American Society for Clinical Pathology

Anatomic Pathology / ORIGINAL ARTICLE

Previous autopsy studies have found the causes of death to be infection (23%-34%), chronic rejection (20%-30%), acute rejection (14%-19%), graft coronary disease (7%-18%), malignant neoplasm (2.5%-10%), and operative complications of transplantation (4%-19%).3,5,6 Several factors may explain our higher rate of death due to complications of transplantation. The autopsy rate for patients dying within 1 month of transplant was 62% (13/21), while the overall rate for our study was 40% (39/97). Clearly, there was a bias toward obtaining autopsies of patients who died soon after transplantation. Our final analysis on the causes of death will, therefore, be skewed toward complications of transplantation. Also, we included deaths due to MSOF as a complication of transplant; similar deaths elsewhere may have been attributed to a terminal infection. Another factor that decreased the deaths attributed to infection was that we isolated a group of patients who died shortly after noncardiac surgery. Other studies may have classified these deaths as due to infection. Only 1 other study identified a cause of death as “complications to surgery, etc,” but no further description of this group was given.5 Previous studies that identified deaths due to chronic rejection used the criteria of coronary fibrointimal hyperplasia, interstitial fibrosis, and diffuse or nodular mononuclear aggregates of inflammation in an interstitial, perivascular, or vascular location.5,6 The deaths that we attributed to GVD could be considered by others as due to chronic rejection. The causes of death in the 6 pediatric patients were right-sided heart failure (2 [33%]), preservation procurement injury (1 [17%]), acute rejection (1 [17%]), GVD (1 [17%]), and unclear (1 [17%]). Although interpretation of our data was hampered by the small sample, our results were not remarkably different from those of another study in which the reported causes of death were as follows: infection, 28%; rejection, 24%; graft coronary disease, 17%; pulmonary hypertension, 14%; nonspecific graft failure, 7%; and other, 10%.2 The 2 infants in our study died within 9 days of transplantation, consistent with the poor infant survival reported elsewhere.1 Two teenaged patients died of sudden cardiac arrest due to GVD and acute rejection (cases 3 and 6, Table 2). In the present study, these cases demonstrated the longest pediatric posttransplantation survival periods of 1.9 and 3.2 years, and both had evidence of coronary artery fibrointimal hyperplasia. GVD has been documented in children as early as 0.2 year after transplantation and has been associated with sudden death.18 Thirteen deaths occurred during the first month after transplantation due to right-sided heart failure (6 [46%]), MSOF (3 [23%]), preservation procurement injury (3 [23%]), and infection (1 [8%]). Other studies have determined that early deaths were due to infection (29%-49%), graft failure (10%-52%), rejection (5%-22%), and © American Society for Clinical Pathology

pulmonary hypertension (15%).3,6,19 Similar results were reported in International Society for Heart and Lung Transplantation data.1 Once again, it is probable that we have sorted deaths differently from other studies. More than half of our patients who died perioperatively or postoperatively without ever becoming medically stable had evidence of pulmonary hypertensive arteriopathy if their lungs were examined at autopsy. Eighty percent (4/5) of patients who died of right-sided heart failure had these changes if their lungs were evaluated. All had grade I to III lesions, and these are thought to be a reversible source of pulmonary hypertension.20-22 Only 2 patients who survived the perioperative period had pulmonary hypertensive arteriopathy. We suggest that even low-grade recipient pulmonary hypertensive arteriopathy is a risk factor for perioperative or postoperative death, particularly death due to right-sided heart failure. Increased preoperative pulmonary vascular resistance, even mild, has been reported elsewhere as an independent predictor of early mortality after transplantation.3,23 Four patients died of MSOF with complicated clinical courses not easily attributed to a single underlying clinical or pathologic event. In all cases, however, transplant operations were complicated by right ventricular dysfunction or hypotension. It is possible that generalized ischemia from the transplant procedure led to injury to multiple organs, which culminated in MSOF and death. Although infections developed in 3 of the patients, the decline in their clinical condition preceded evidence of infection. Thus, infection was thought to be a result of MSOF and not the primary cause of death. Interestingly, 50% (2/4) of the patients with MSOF had pulmonary hypertensive arteriopathy that may have contributed to cardiac dysfunction after transplantation. Right-sided heart failure was the main cause of perioperative and postoperative mortality during the first 9 years of the heart transplant program at DUMC (Table 4). Subsequently, MSOF became the leading cause of these deaths. This change suggests that better patient selection to exclude those with pulmonary hypertension or improved postoperative care occurred after those early years. Perhaps some of the patients who died of MSOF had cardiac dysfunction similar to that in patients who died earlier of right-sided heart failure; improved clinical management may have kept them alive long enough to develop MSOF. Deaths 1 month to 2 years after transplantation accounted for 9 patients and were due to infection (3 [33%]), acute rejection (2 [22%]), MSOF (1 [11%]), cardiac arrhythmia (1 [11%]), unclear cause (1 [11%]), and other cause (1 [11%]). These results are similar to those reported elsewhere for deaths up to 1 year after transplantation.1,6,19 Seventeen patients died more than 2 years after transplantation due to complications of noncardiac surgery (3 Am J Clin Pathol 2003;119:740-748 745 DOI: 10.1309/713JVR58EF916DN5

745 745

Alexander and Steenbergen / DEATH AFTER CARDIAC TRANSPLANTATION

[18%]), malignant neoplasm (3 [18%]), GVD (3 [18%]), infection (1 [6%]), acute rejection (1 [6%]), cardiac arrhythmia (1 [6%]), unclear cause (2 [12%]), and other cause (3 [18%]). Other studies have found the leading causes of death in these longer term survivors were GVD, malignant neoplasm, and nonspecific graft failure; other causes included infection, acute rejection, and MSOF.1,6,19 Complications of noncardiac surgery were a leading cause of death not commented on in many other studies. Transplant recipients have increased susceptibility to infection and delayed healing secondary to the use of corticosteroids and immunosuppressive medications. There is an increased likelihood that transplant recipients will require surgery owing to an increased incidence of various malignant neoplasms.24-26 We believe this highlights the importance of careful monitoring and treatment for infection after surgery for any cause. Infections after transplantation follow a well-established pattern: the usual postsurgical infections and pneumonia are seen during the first month; opportunistic infections predominate from 1 to 6 months; normal community-acquired infections, some opportunistic infections such as CMV colitis and retinitis, and viral driven malignant neoplasms (eg, posttransplant lymphoproliferative disorder) are most common subsequently.27 Our data followed these trends. Both patients with fatal CMV infections died within 3 months of a grade 3A rejection episode. The fatal Aspergillus infection did not seem related to recent rejection. It seems likely that increased immunosuppression following clinically significant rejection predisposed to lethal opportunistic infection. In fact, 5% to 10% of infections beyond 6 months after transplantation are opportunistic and due to increased immunosuppression secondary to recurrent or chronic rejection.27 Five patients had renal adenomas or carcinomas found incidentally at autopsy. All arose in the setting of nephrosclerosis, and all were papillary neoplasms of low nuclear grade. The lesions called adenomas varied from 0.2 to 1.2 cm in diameter. The carcinomas were up to 1.5 cm. It is debatable how to classify small renal epithelial neoplasms found incidentally at autopsy,28-31 but it is clear that they are more common in sclerotic or end-stage kidneys.32,33 Given the similar size and histologic features of these tumors, as well as the lack of metastatic disease, it is probably best to consider them renal neoplasms of uncertain malignant potential. Although malignant neoplasms were documented in 10 patients (26%), only 3 (8%) died because of them. If we exclude the colonic carcinoma that was clearly incidental to transplantation, renal neoplasms, and the DFSP that occurred and recurred before transplantation, the incidence of malignant neoplasm drops to 18% (7/39), which is higher than the incidence of 3% to 16% reported elsewhere.24,34-36 The rate of malignant neoplasm, however, is dependent on the length of follow-up after transplantation.24,26,34,35,37,38 The most 746 746

Am J Clin Pathol 2003;119:740-748 DOI: 10.1309/713JVR58EF916DN5

common malignant neoplasms after transplantation were skin cancers; 3 patients were diagnosed with a total of 6 squamous cell carcinomas and 1 basal cell carcinoma. An increased incidence of skin cancer, with a predominance of squamous cell carcinoma, has been well documented in heart allograft recipients.24,25,34,35,37,39 Lymphoma developed in 8% (3/39) of the patients; non-Hodgkin lymphoma and Hodgkin lymphoma accounted for 2 and 1 of these cases, respectively. Lymphomas are reported as the most common noncutaneous malignant neoplasms, with an incidence of 2% to 16% for non-Hodgkin lymphoma and 0.4% for Hodgkin lymphoma.24,34,35,37 Colonic and prostate carcinoma were found in 1 patient each, while squamous cell carcinomas of the lung were found in 2. Although these carcinomas are reported by Penn24 as having no increased incidence in the transplant population, more recent data suggest that the incidence of lung and prostate carcinoma increases linearly more than 1 year after transplantation.36 The time to development of the malignant neoplasm was quite variable. If we exclude the colonic carcinoma, DFSP, and renal neoplasms, the first malignant neoplasm seen was Kaposi sarcoma at 2.4 years after transplantation. Kaposi sarcoma generally is the first tumor seen and appears on average at 19 to 22 months after transplantation.24,36 Lymphomas were diagnosed 4.6 to 7.2 years after transplantation. Although 2 of the lymphomas occurred after the average of 32 to 56 months, both fell well within the published range of 0.5 to 254 months.24,35,36 GVD was the cause of death in 3 patients. All had severe fibrointimal hyperplasia and evidence of acute and/or old ischemic injury to the heart. Two had interstitial and perivascular fibrosis. Such fibrosis may be the result of ischemic injury at the time of transplantation or previous bouts of rejection.10,40 Both of these patients had a history of 3 or more episodes of grade 3 rejection revealed by endomyocardial biopsy. The last patient had 1 previous episode of grade 3A rejection. None of the aforementioned patients had evidence of more than grade 1A rejection at autopsy or on their most recent endomyocardial biopsy. Thus, all 3 of the patients had at least 1 episode of grade 3 acute rejection revealed by a previous biopsy. The relationship of acute cellular rejection to the development of coronary disease as reported in the literature is controversial; some studies have found a relationship between the two,41-50 while others have not.51,52 Seven patients (18%) who were in medically stable condition died of sudden cardiac arrests, all more than 1 month after transplantation. They accounted for 27% (7/26) of the deaths after 1 month. Sudden cardiac death has been reported to occur in 10% of cardiac transplant recipients, with coronary artery disease being found in most of them.53 Our higher rate of sudden cardiac death may be due in part to the increased likelihood of an autopsy being performed on © American Society for Clinical Pathology

Anatomic Pathology / ORIGINAL ARTICLE

a patient who died suddenly and unexpectedly. Sudden cardiac death has been attributed to acute rejection and myocardial infarction.53,54 The fatal arrhythmia commonly was bradycardia or atrial tachyarrhythmia.53,55 The conduction system may be damaged by acute rejection, leading to arrhythmias and sudden death.56 Of the 5 patients who died of a sudden cardiac arrest not due to acute rejection, 3 (60%) had a history of 3 or more episodes of grade 3 rejection. In contrast, of the remaining 23 patients with complete biopsy rejection histories at DUMC, only 5 (22%) had this amount of rejection. No grade 4 rejection episodes were recorded for any of the patients. Three of the above 5 patients were undergoing hemodialysis or plasmapheresis. Another patient who died suddenly during hemodialysis had a complex medical history, mild coronary atherosclerosis, and 3 past episodes of grade 3 rejection. Although her cause of death was unclear, it is possible that she died of a fatal arrhythmia. We speculate that repeated episodes of grade 3 rejection may have damaged the conduction system. These hearts then were at increased risk of developing arrhythmias during the hemodynamic stresses of hemodialysis or plasmapheresis. Acute rejection accounted for 2 deaths due to sudden cardiac arrest. The patients were 17 and 46 years old, and both had a history of depression. We suggest factors that increased the risk of noncompliance with the antirejection medication regimen, such as depression, pose a significant risk factor for death due to acute rejection. From the Department of Pathology, Duke University Medical Center, Durham, NC. Address reprint requests to Dr Alexander: Dept of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710. Acknowledgments: We thank Alan Proia, MD, PhD, for his insights on renal neoplasia found incidentally at autopsy; Louis DiBernardo, MD, for his help with clinical and pathologic correlation; and Thomas Cummings, MD, for commentary on the manuscript.

References 1. Hosenpud JD, Bennett LE, Keck BM, et al. The Registry of the International Society for Heart and Lung Transplantation: eighteenth Official Report: 2001. J Heart Lung Transplant. 2001;20:805-815. 2. Sarris GE, Smith JA, Bernstein D, et al. Pediatric cardiac transplantation: the Stanford experience. Circulation. 1994;90(5 pt 2):II51-II55. 3. Sarris GE, Moore KA, Schroeder JS, et al. Cardiac transplantation: the Stanford experience in the cyclosporine era. J Thorac Cardiovasc Surg. 1994;108:240-252. 4. Thornton CM, Hill CM. Heart transplantation: an autopsy review. Ir Med J. 1990;83:137-138. 5. Graham AR. Autopsy findings in cardiac transplant patients: a 10-year experience. Am J Clin Pathol. 1992;97:369-375.

© American Society for Clinical Pathology

6. Rose AG, Viviers L, Odell JA. Autopsy-determined causes of death following cardiac transplantation: a study of 81 patients and literature review. Arch Pathol Lab Med. 1992;116:1137-1141. 7. Suwangool P, Ongcharit C, Kurowat Y, et al. Heart transplant pathology: the Thai experience at Chulalongkorn University Hospital. Transplant Proc. 1994;26:2317. 8. McManus BM. Allografts and autopsies: critical opportunities for insight [editorial]. Am J Clin Pathol. 1992;97:301-303. 9. Billingham ME. Histopathology of graft coronary disease. J Heart Lung Transplant. 1992;11(3 pt 2):S38-S44. 10. Billingham ME. The pathologic changes in long-term heart and lung transplant survivors. J Heart Lung Transplant. 1992;11(4 pt 2):S252-S257. 11. Cary NR. Diagnostic criteria of chronic rejection in transplanted hearts. Transplant Proc. 1993;25:2026-2028. 12. Wright JN, Salem D. Sudden cardiac death and the “athlete’s heart.” Arch Intern Med. 1995;155:1473-1480. 13. Liberthson RR. Sudden death from cardiac causes in children and young adults. N Engl J Med. 1996;334:1039-1044. 14. Heath D, Edwards JE. The pathology of hypertensive pulmonary vascular disease: a description of six grades of structural changes in the pulmonary arteries with special references to congenital cardiac septal defects. Circulation. 1958;18:533-547. 15. Billingham ME, Cary NR, Hammond ME, et al, for the International Society for Heart Transplantation. A working formulation for the standardization of nomenclature in the diagnosis of heart and lung rejection: Heart Rejection Study Group. J Heart Transplant. 1990;9:587-593. 16. Haft JI. Cardiovascular injury induced by sympathetic catecholamines. Prog Cardiovasc Dis. 1974;17:73-86. 17. Fagiuoli S, Minniti F, Pevere S, et al. HBV and HCV infections in heart transplant recipients. J Heart Lung Transplant. 2001;20:718-724. 18. Pahl E, Zales VR, Fricker FJ, et al. Posttransplant coronary artery disease in children: a multicenter national survey. Circulation. 1994;90(5 pt 2):II56-II60. 19. Winters GL, Schoen FJ. Pathology of cardiac transplantation. In: Silver MD, Gotlieb AI, Schoen FJ, eds. Cardiovascular Pathology. 3rd ed. New York, NY: Churchill Livingstone; 2001:725-762. 20. Rabinovitch M, Keane JF, Norwood WI, et al. Vascular structure in lung tissue obtained at biopsy correlated with pulmonary hemodynamic findings after repair of congenital heart defects. Circulation. 1984;69:655-657. 21. Wagenvoort CA, Wagenvoort N, Draulans-Noe Y. Reversibility of plexogenic pulmonary arteriopathy following banding of the pulmonary artery. J Thorac Cardiovasc Surg. 1984;87:876-886. 22. Wagenvoort CA. Open lung biopsies in congenital heart disease for evaluation of pulmonary vascular disease: predictive value with regard to corrective operability. Histopathology. 1985;9:417-436. 23. Delgado JF, Gomez-Sanchez MA, Saenz de la Calzada C, et al. Impact of mild pulmonary hypertension on mortality and pulmonary artery pressure profile after heart transplantation. J Heart Lung Transplant. 2001;20:942-948. 24. Penn I. Incidence and treatment of neoplasia after transplantation. J Heart Lung Transplant. 1993;12(6 pt 2):S328-S336. 25. Fortina AB, Caforio ALP, Piaserico S, et al. Skin cancer in heart transplant recipient: frequency and risk factor analysis. J Heart Lung Transplant. 2000;19:249-255.

Am J Clin Pathol 2003;119:740-748 747 DOI: 10.1309/713JVR58EF916DN5

747 747

Alexander and Steenbergen / DEATH AFTER CARDIAC TRANSPLANTATION

26. Curtil A, Robin J, Tronc F, et al. Malignant neoplasms following cardiac transplantation. Eur J Cardiothorac Surg. 1997;12:101-106. 27. Fishman JA, Rubin RH. Infection in organ-transplant recipients. N Engl J Med. 1998;338:1741-1751. 28. Bell ET. Renal Diseases. 2nd ed. Philadelphia, PA: Lea & Febiger; 1950. 29. Eble JN, Warfel K. Early human renal cortical epithelial neoplasia [abstract]. Mod Pathol. 1991;4:45A. 30. Aizawa S, Suzuki M, Kikuchi Y, et al. Clinocopathological study of small renal cell carcinomas with metastases. Acta Pathol Jpn. 1987;37:947-954. 31. Suzuki M, Nikaido T, Ikegami M, et al. Renal adenoma: clinicopathological and histochemical studies. Acta Pathol Jpn. 1989;39:731-736. 32. Budin RE, McDonnell PJ. Renal cell neoplasms: their relationship to arteriolonephrosclerosis. Arch Pathol Lab Med. 1984;108:138-140. 33. Hughson MD, Hennigar GR, McManus JFA. Atypical cysts, acquired renal cystic disease, and renal tumors in end stage dialysis kidneys. Lab Invest. 1980;42:475-480. 34. Mihalov ML, Gattuso P, Abraham K, et al. Incidence of posttransplant malignancy among 674 solid-organ-transplant recipients at a single center. Clin Transpl. 1996;10:248-255. 35. Cardillo M, Rossini G, Scalamongna M, et al. Tumor incidence in heart transplant patients: report of the North Italy Transplant Program Registry. Transplant Proc. 2001;33:1840-1843. 36. Yancy CW, Naftel DC, Renlund D, et al. Malignancy and death from malignancy after heart transplantation, a multicenter time related risk analysis. J Heart Lung Transplant. 2002;21:55. Abstract 2. 37. Tenderich G, Deyerling W, Schulz U, et al. Malignant neoplastic disorders following long-term immunosuppression after orthotopic heart transplant. Transplant Proc. 2001;33:3653-3655. 38. Hertz MI, Taylor DO, Trulock EP, et al. The registry of the international society for heart and lung transplantation: nineteenth official report: 2002. J Heart Lung Transplant. 2002;21:950-970. 39. Lampros TD, Cobanoglu A, Parker F, et al. Squamous and basal cell carcinoma in heart transplant recipients. J Heart Lung Transplant. 1998;17:586-591. 40. Rowan RA, Billingham ME. Pathologic changes in the longterm transplanted heart: a morphometric study of myocardial hypertrophy, vascularity, and fibrosis. Hum Pathol. 1990;21:767-772. 41. Uretsky BF, Murali S, Reddy S, et al. Development of coronary artery disease in cardiac transplant patients receiving immunosuppressive therapy with cyclosporine and prednisone. Circulation. 1987;76:827-834. 42. Narrod J, Kormos R, Armitage J, et al. Acute rejection and coronary artery disease in long term survivors of heart transplantation. J Heart Transplant. 1989;8:418-420.

748 748

Am J Clin Pathol 2003;119:740-748 DOI: 10.1309/713JVR58EF916DN5

43. Olivari MT, Homans DC, Wilson RF, et al. Coronary artery disease in cardiac transplant patients receiving triple-drug immunosuppressive therapy. Circulation. 1989;80(5 pt 2):III111-III115. 44. Schutz A, Kemkes BM, Kugler C, et al. The influence of rejection episodes on the development of coronary artery disease after heart transplantation. Eur J Cardiothorac Surg. 1990;4:300-307. 45. Radovancevic B, Poindexter S, Birovljev S, et al. Risk factors for the development of accelerated coronary artery disease in cardiac transplant patients. Eur J Cardiothorac Surg. 1990;4:309-312. 46. Winters GL, Kendall TJ, Radio SJ, et al. Posttransplant obesity and hyperlipidemia: major predictors of severity of coronary arteriopathy in failed human heart allografts. J Heart Transplant. 1990;9:364-371. 47. Winters GL, Johnson MR, Costanzo-Nordin MR. Prognostic significance of mild acute rejection [abstract]. Mod Pathol. 1991;4:22A. 48. Zerbe T, Uretsky B, Kormos R, et al. Graft atherosclerosis: effects of cellular rejection and human lymphocyte antigen. J Heart Lung Transplant. 1992;11(3 pt 2):S104-S110. 49. Liu G, Butany J. Morphology of graft arteriosclerosis in cardiac transplant recipients. Hum Pathol. 1992;23:768-773. 50. Kobashigawa JA, Miller L, Yeung A, et al, for Sandoz/CVIS Investigators. Does acute rejection correlate with the development of transplant coronary artery disease? a multicenter study using intravascular ultrasound. J Heart Lung Transplant. 1995;14(6 pt 2):S221-S226. 51. Gao S, Schroeder JS, Hunt SA, et al. Influence of graft rejection on incidence of accelerated graft coronary artery disease: a new approach to analysis. J Heart Lung Transplant. 1993;12:1029-1035. 52. Stovin PG, Sharples L, Schofield PM, et al. Lack of association between endomyocardial evidence of rejection in the first six months and the later development of transplantrelated coronary artery disease. J Heart Lung Transplant. 1993;12:110-116. 53. Patel VS, Lim M, Massin EK, et al. Sudden cardiac death in cardiac transplant recipients. Circulation. 1996;94(9 suppl):II273-II277. 54. Gao SZ, Schroeder JS, Hunt SA, et al. Acute myocardial infarction in cardiac transplant recipients. Am J Cardiol. 1989;64:1093-1097. 55. Grinstead WC, Smart FW, Pratt CM, et al. Sudden death caused by bradycardia and asystole in a heart transplant patient with coronary arteriopathy. J Heart Lung Transplant. 1991;10:931-936. 56. Foerster A. The conduction system in human cardiac allografts: a histological and immunopathological study. Pathol Res Pract. 1992;188:783-790.

© American Society for Clinical Pathology

Suggest Documents