0.52 ± 0.07 histone cluster 2, H2be. N33927. HIST1H2BD. 0.51 ± 0.07 histone cluster 1, H2bd. AI653010. HIST1H4J. 0.49 ± 0.05 histone cluster 1, H4j.
Supplemental Table S2: Genes negatively regulated by shear stress in human T/C28a2 chondrocytes GOC
EST
Gene Symbol
Shear/Static (Fold ± SD)
Description
pericentriolar material 1 structural maintenance of chromosomes 4 structural maintenance of chromosomes 4 H2A histone family, member V histone cluster 3, H2a myristoylated alanine-rich protein kinase C substrate histone cluster 1, H2ac histone cluster 1, H4c histone cluster 2, H2be histone cluster 1, H2bd histone cluster 1, H4j histone cluster 1, H2bl H1 histone family, member 0 H1 histone family, member 0 glypican 6 nebulin
Cell adhesion and cytosheleton AI168304 AA452095 AA455239 H97000 AI268551 AA131421
PCM1 SMC4 SMC4 H2AFV HIST3H2A MARCKS
0.60 ± 0.11 0.53 ± 0.08 0.56 ± 0.06 0.55 ± 0.01 0.54 ± 0.11 0.55 ± 0.12
N50797 AA868008 AA010223 N33927 AI653010 AI340654 H57830 W69399 AA456147 AA176957
HIST1H2AC HIST1H4C HIST2H2BE HIST1H2BD HIST1H4J HIST1H2BL
0.53 ± 0.07 0.52 ± 0.08 0.52 ± 0.07 0.51 ± 0.07 0.49 ± 0.05 0.49 ± 0.07 0.47 ± 0.02 0.45 ± 0.05 0.57 ± 0.05 0.24 ± 0.06
H1F0
H1F0 GPC6
NEB
Cell growth and differentition R39089
PPM1B
0.62 ± 0.06
H73329
TPX2
0.61 ± 0.05
H81024 AA421171
AURKB NUF2
0.62 ± 0.12 0.61 ± 0.10
W95001 AA446462
CDC25C BUB1
0.61 ± 0.12 0.60 ± 0.09
AA598974 AA278384 AA459213 AA459213 AA400476 AA682321
CDC2
NEK3
0.58 ± 0.06 0.58 ± 0.07 0.61 ± 0.09 0.61 ± 0.12 0.58 ± 0.09 0.56 ± 0.08
W93379
NEK2
0.55 ± 0.06
AA262211 AA701455 AA504625 W93717 AA620485 T87341 AA292054
DLG7 CENPF
0.53 ± 0.05 0.51 ± 0.05 0.54 ± 0.12 0.50 ± 0.03 0.50 ± 0.07 0.49 ± 0.05 0.48 ± 0.03
CDC2 CCNA2 CCNA2
KIF2C
KIF11
DLG7 NUSAP1 CENPF GAS1
protein phosphatase 1B (formerly 2C), magnesium-dependent beta isoform TPX2, microtubule-associated, homolog (Xenopus laevis) aurora kinase B NUF2, NDC80 kinetochore complex component, homolog cell division cycle 25 homolog C BUB1 budding uninhibited by benzimidazoles 1 homolog cyclin-dependent kinase 1 cell division cycle 2, G1 to S and G2 to M cyclin A2 cyclin A2 kinesin family member 2C NIMA (never in mitosis gene a)-related kinase 3 NIMA (never in mitosis gene a)-related kinase 2 large homolog 7 (Drosophila) centromere protein F kinesin family member 11 discs, large homolog 7 (Drosophila) nucleolar and spindle associated protein 1 centromere protein F growth arrest-specific 1
N72115
CDKN2C
0.48 ± 0.02
cyclin-dependent kinase inhibitor 2C
BCCIP MEIS2 TRPS1 TRPS1
0.61 ± 0.07 0.56 ± 0.03 0.54 ± 0.07 0.50 ± 0.06
BRCA2 and CDKN1A interacting protein Meis homeobox 2 trichorhinophalangeal syndrome I trichorhinophalangeal syndrome I
PBK BMP5 VCAM1 CXCL12 CXCL12 NR2F2
0.59 ± 0.08 0.57 ± 0.03 0.41 ± 0.07 0.38 ± 0.09 0.26 ± 0.05 0.57 ± 0.04
PSIP1
PDZ binding kinase bone morphogenetic protein 5 vascular cell adhesion molecule 1 chemokine (C-X-C motif) ligand 12 chemokine (C-X-C motif) ligand 12 nuclear receptor subfamily 2, group F, member 2 PC4 and SFRS1 interacting protein 1 nuclear factor I/A nuclear factor I/A
Cell survival/death T86027 AA148641 AI276654 R54193 Signaling transduction AA476576 AA779457 H07071 AI655374 AA447115 AA418544 AA228130 AA456289 AA598615
NFIA
NFIA
0.56 ± 0.03 0.59 ± 0.07 0.56 ± 0.05
AA425404
FAM64A
0.60 ± 0.06
AI002036
ANP32E
0.58 ± 0.04
AA962465 AA682502 W72033 AA699878 AI034132 AA452877 H14208 AA053865 AA620527
NUCKS1
BCL2L11 DIRAS3 HTRA1 PDGFC STOX1 PALM TRIB2 PRKAB2
0.57 ± 0.05 0.55 ± 0.06 0.40 ± 0.11 0.40 ± 0.10 0.44 ± 0.06 0.57 ± 0.06 0.55 ± 0.10 0.55 ± 0.10 0.55 ± 0.10
AA054287 AI005521
RBM3 DNAJC15
0.54 ± 0.07 0.54 ± 0.08
H18956
GALNTL1
0.54 ± 0.07
AA137266
NUCKS1
0.53 ± 0.03
AA485453
NUCKS1
0.55 ± 0.06
AA443127
NUCKS1
0.53 ± 0.05
H10788
CIT
0.53 ± 0.05
AA419251 AA913480
IFITM1 KLRC1
0.52 ± 0.06 0.52 ± 0.06
Others family with sequence similarity 64, member A acidic (leucine-rich) nuclear phosphoprotein 32 family, member E similar to PRO2870 BCL2-like 11 (apoptosis facilitator) GTP-binding RAS-like 3 HtrA serine peptidase 1 platelet derived growth factor C storkhead box 1 paralemmin tribbles homolog 2 (Drosophila) protein kinase, AMP-activated, beta 2 non-catalytic subunit RNA binding motif (RNP1, RRM) protein 3 DnaJ (Hsp40) homolog, subfamily C, member 15 UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferaselike 1 nuclear casein kinase and cyclin-dependent kinase substrate 1 nuclear casein kinase and cyclin-dependent kinase substrate 1 nuclear casein kinase and cyclin-dependent kinase substrate 1 citron (rho-interacting, serine/threonine kinase 21 interferon induced transmembrane protein 1 killer cell lectin-like receptor subfamily C
AI017211
FAM172A
0.52 ± 0.04
AA481250 AA504348 AA026682 AA504348
CHI3L2 TOP2A
0.61 ± 0.06 0.51 ± 0.04 0.53 ± 0.08 0.51 ± 0.07
TOP2A TOP2A
Unkown R94504 R59173 AI239950 AA677210 AI204339 AI191110 H11968 AI076718 H05961 AI184169 AA779949 AA406371 AI095013 AA458633 AA496022
0.61 ± 0.11 0.60 ± 0.09 0.59 ± 0.06 0.58 ± 0.03 0.58 ± 0.07 0.56 ± 0.06 0.56 ± 0.09 0.54 ± 0.08 0.54 ± 0.06 0.53 ± 0.07 0.51 ± 0.03 0.51 ± 0.05 0.51 ± 0.09 0.49 ± 0.08 0.39 ± 0.09
family with sequence similarity 172, member A chitinase 3-like 2 topoisomerase (DNA) II alpha 170kDa topoisomerase (DNA) II alpha 170kDa topoisomerase (DNA) II alpha 170kDa
