... and 5´-GGGAAGGCCACAGACACATC-3´ for MYOG,. 5´-CGTCAAAGGTGAAGCAGGAC-3´ and 5´-CTCCAGTGCCCGTAGATCAG-3´ for NDUFA13, and.
Stem Cell Reports, Volume 7
Supplemental Information
Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle Akiyoshi Uezumi, Masashi Nakatani, Madoka Ikemoto-Uezumi, Naoki Yamamoto, Mitsuhiro Morita, Asami Yamaguchi, Harumoto Yamada, Takehiro Kasai, Satoru Masuda, Asako Narita, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, So-ichiro Fukada, Ichizo Nishino, and Kunihiro Tsuchida
A
Myogenic
*
350
*
300
Number of cells / field
Number of cells / field
350
Mesenchymal
250 200
*
150 100 50
300
200 150 100 50
0
0
Non
B
*
1.4
Col I
Col IV
Fib
Lam
Non
Myogenic
Col I
Col IV
Fib
Lam
Mesenchymal
*
1.4 1.2
Relative absorbance
1.2
Relative absorbance
*
250
1 0.8 0.6 0.4 0.2
1 0.8 0.6 0.4 0.2
0
0
-
bFGF EGF EGF IGF-I IGF-I LIF 2.5 2.5 10 2.5 10 2.5
LIF 10 (ng/ml)
Figure S1
-
bFGF EGF EGF IGF-I IGF-I LIF 2.5 2.5 10 2.5 10 2.5
LIF 10 (ng/ml)
4
10
2
4
5
5
2
10 5
5
2
4
3
10
4
10
5
10
4
10
5
2
3
4
5
10 5
5
0
10
3
10
4
10
4
10
5
4
3
10
4
10
5
10
2
3
4
5
10
5
10 4
5
10 4
10
2
3
10
4
10
HLA-A,B,C
10
5
3
0
0
10
10 4
10
0
0
46
5
10 5
2
10 2
3
10 3
CX3CR1
10 2
10
10 2
10
10 4
10
0
2
10 3
10 4
10
10 3
0.0428
10 5
10 4
0
10 3
CD352 (NTB-A)
10 2
2
3
4
5
0
10
10
10
10
10 5
0
0.214
10 5
0
10
10
10
10
10
4
CD324 (E-Cadherin)
10
3
0
2
4
10 3
10
10
0
0.0216
5
10 2
0
10
10 2
10 3
10
10
5
2
CD298
10
2
0
10
10 3
10 4
2
0
10
100
10 4
10 3
10 5
10 5
0
3
CD273 (B7-DC)
10
0
0
2
10 2
10
10 4
10 3
10 5
10 2
10 3
10 4
20.4
10
2
0
10
10 3
10 5
0
2
CD254
10
0.0667
10 5
2
3
4
5
10 4
2
0
10
10 3
0
10 4
Blank
10 3
10
10
10
10
10 4
10 2
10
4
10
3
10
4
10 3
10 4
0.221
10 4
CD80
10 3
10
2
10
3
10
4
0.153
CD220
10 2
10 2
10 4
0.238
10 3
0.106
CD51
10
CD62L
10 2
2
5
10
2
10
3
10
4
10
2
10
3
10
4
0.109
CD274 (B7-H1)
10
10
10
10
10
4
10 2
10 3
10 4
0.151
CD325
10
3
0
0
10 3
10 4
2
10
3
10
4
HLA-A2
10
10
5
10 5
0.0853
CXCR7
10 2
0.24
5
5
5
10 5
10
CD354 (TREM-1)
0
0
2
0.175
CD300e (IREM-2)
0
0
94.6
5
10 5
10 5
10 5
10
CD255 (TWEAK)
0
0
0
0
10
99.9
5
2
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
10 3
10
5
0
10 2
10 3
10 4
10
0
10 2
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
0.0427
3
2
0
10
10 3
10 4
10
5
0
10 2
10 5
10 3
10 5
0
10
10
10
10
10 4
CD79b
10 3
10
5
10 5
0.0216
10 4
CD33
10 3
0.0872
10
5
4
5
10 3
10
10
0
10 4
0
10 2
10
4
CD21
10
3
5
10 5
0.0222
10 4
CD10
10 3
96.5
10
10 5
0
0
10 5
10 2
0.577
10 4
10 3
10 2
10 3
10 4
10 5
10 3
CD62E
5
10 4
10
0
10 2
4
4
10 2
0
2
10
CD45RA
10
10 2
10
2
3
CD1a
10
10 5
10 3
10
0
2
10 2
10
4
0
0
0
0
0
10 5
0
0
0.0849
CD50 (ICAM-3)
3
5
10 2
10 3
10 4
10
10
0.0427
CD45
10
2
10 3
10
0
2
2
5
10 4
10
0
10
10
10 5
0.0641
10 4
CD32
10 3
0
10
2
10 3
10 2
0
0
10 2
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
0.0659
10
5
10 4
2
10 3
0
10
CD20
10
4
10 5
10
3
2
10 3
10 4
10
10
10 4
0
2
0.151
5
10 5
0
10
10 3
10 4
10
5
10 4
CD9
10 3
4
5
10 3
10
10
0
100
10
10 2
10 2
4
0
0
10
Blank
3
10 2
10 3
10
10
10
0
0
4
10 2
10 3
10
10 5
0.216
4
10 3
10
10 5
0
0.0434
10 2
10 3
10
10 5
10 2
2
3
10
4
2
10 4
10
4
10
10
3
10
4
10
2
10
3
93
10
4
10
10
10 2
10 5
10 2
10 2
10 3
10 4
0.242
10 4
CD81
10 3
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
10
10
10
3
10
4
10
0.0861
CD300F
10
0.153
CD275 (B7-H2)
10
0.109
CD257 (BAFF)
10
5
5
5
10 2
10 3
10 4
10 5
10 2
10 3
10 4
0
2
10
3
10
4
HLA-DQ
10
10
0.0215
5
10 5
δ-Opioid Receptor
0
0.353
CD355 (CRTAM)
0
0.0659
CD326 (Ep-CAM)
0
0
0
0
0.683
5
10 5
10 5
CD221 (IGF-1R)
0
0
10 4
100
10 3
5
5
CD62P (P-selectin)
0
0.149
5
10 5
0.0216
10 4
CD34
10 3
5
10 5
0.0878
CD22
10
3
0.13
10 3
CD45RB
10
10 2
10
2
10
0.0222
CD1b
10
CD11a
10 2
10
CD51/61
0
0
0
0
0
0
0.111
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
2
3
10
4
10
2
10 2
10
2
10 4
10
4
10
10
3
10
4
0.109
10 4
CD35
10 3
10
2
10 2
10 2
10 2
10
3
10
4
10 4
10 3
10 4
5
10 5
10 5
10
10 5
0.0874
10 4
CD82
10 3
98.9
10 3
CD63
99.9
CD52
10
5
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
10
10
10
3
10
4
10
0.0887
CD301
10
1.02
CD276
10
100
CD258 (LIGHT)
10
0
0
0
10 3
10 4
2
10 4
10
3
10
4
0.149
DLL1
10 3
HLA-DR
10
10 2
0
CD357 (GITR)
10 2
0.239
5
5
5
5
10
5
10 5
10 5
CD328 (Siglec-7)
0
0
0
0
0.0653
CD226 (DNAM-1)
0
0
0
0
0.044
5
10 5
0.0444
CD23
10
3
5
10 5
10
0.0218
10 3
0.149
CD1c
10
CD11b
10 2
10
CD45RO
0
0
0
0
0
0.109
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
2
10 2
10
3
10
4
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
2
10
4
3
10
4
3
10
4
10 4
5
10 5
10
10 3
10 4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
10
10
3
10
4
10
10
10 3
10 4
2
10 4
10
3
10
4
0.212
DLL4
10 3
HLA-E
10
10 2
10 5
10
5
10 5
0.0643
CD360 (IL-21R)
10 2
8.96
5
5
5
5
10 5
CD334 (FGFR4)
10
2
0.13
CD303
10
10 5
0.0222
CD277
10
0.769
5
5
10 5
10
10
0.0889
CD261 (DR4)
10
5
10 5
10
0.0839
10 4
CD83
10 3
0.472
10 3
CD64
0.066
CD53
10
0.111
CD46
10
35.1
10 4
CD36
10 3
0.132
CD24
10
3
10 4
CD229 (Ly-9)
10 2
10 2
10 2
10
10
10 2
10
2
78
10 3
0.196
CD1d
10
CD11b (activated)
0
0
0.0627
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
3
10
4
2
2
10 2
10
10
10 2
10
2
10 4
10
4
10
3
10
4
3
10
4
0
0
0
0
0
0
0
10 3
10 4
0.147
10 4
CD84
10 3
2
10
3
10
4
5
5
5
10 5
10
10
10 5
2
10
3
10
4
2
10
3
10
4
10
3
10
4
10
10
10
10 2
10 3
10 4
100
5
5
5
5
10 5
CD335 (NKp46)
10
2
0.022
CD304
10
88
CD278 (ICOS)
10
10
10 5
10 5
0.0654
CD262 (DR5)
10
63.1
CD231 (TALLA)
10 2
10 2
10 4
0.108
10 3
0.129
CD54
10
3.04
CD47
10
100
10 4
CD38
10 3
0.0654
CD25
10
3
5
10 5
10
0.0438
10 3
0.154
CD2
10
CD11c
10 2
10
CD66a/c/e
0
0
0
0
0
0
0
0.109
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
10 2
10 3
10 4
0
2
10
3
10
4
HLA-G
10
10
0.0638
5
10 5
DR3 (TRAMP)
0
0.0222
β2-microglobulin
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
2
2
2
3
10
4
10
4
3
10
4
3
10
4
10 4
10 2
10 4
10 3
10 4
0.129
10 3
CD85a (ILT5)
10 2
10
0
0
0
0
0
0
0
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
10
3
10
4
10
10
10
10 4
10 3
10 4
99.2
BTLA
10 3
2
10
3
10
4
0.35
EGFR
10 2
10 2
0.107
5
5
5
10
5
10 5
10 5
CD336 (NKp44)
10
2
0.109
CD307
10
10
5
10 5
10 5
0.0439
CD279 (PD-1)
10
5
5
10 5
10
10
0.0883
CD263 (DcR1)
10
0.11
5
10 5
0.0214
10 3
0.133
CD55
10
75.6
CD48
10
0.219
10 4
CD39
10 3
5
10 5
10
0.0658
CD26
10
3
80.1
10 4
CD13
10 3
100
CD3
10
CD66b
10 2
10
10
10 2
10
2
10 2
10
CD235ab
0
0
0
0
0
0
0
0
0
0.0206
IFN-γ R β chain
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
2
3
10
4
10
4
10
3
10
4
52.7
10 4
CD40
10 3
0.438
CD27
10
3
0
10 4
CD14
10 3
0.155
CD4
10
2
10
3
10
4
10 4
10 3
10 4
2
10
3
10
4
2
10
3
10
4
2
10
10
10
3
10
4
10
10
10
3
10
4
10
0.0222
10
10 2
10 2
10 4
10 3
10 4
0.262
C3AR
10 3
0.308
10 5
10 5
5
5
5
5
10 5
10 5
CD337 (NKp30)
10
2
5
5
10 5
CD307d (FcRL4)
10
5
10 5
10
0.0888
CD282 (TLR2)
10
0.281
CD266 (Fn14)
10
29.3
CD243
10 2
10 4
0.176
10 3
0.171
10 3
CD69
CD85d (ILT4)
10 2
10 2
5
10 5
10
0.0432
CD56 (NCAM)
10
97.4
CD49a
10
10 2
10
2
10 2
10
0.0445
5
3
0
10
2
10
3
0
10
4
10
5
Ig light chain κ
0
10 2
10
10 4
10
erbB3/HER-3
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
2
3
10
4
2
10
4
10 4
10
3
10
100
4
CD41
10 3
0
CD28
10
3
0.592
10 4
CD15
10 3
3
10
4
10 4
10 3
10 4
2
10
3
10
4
2
10
3
10
4
10
2
10
10
3
10
4
0
0
0
0
10 5
10
10
10
10
3
10
4
10
0.0431
10 4
10 3
10 4
0.066
C5L2
10 3
10
2
10
3
10
4
10
5
10 5
10 5
0.0446
FcεRlα
10 2
10 2
0.624
5
5
5
5
10 5
CD338 (ABCG2)
10
2
5
5
10 5
10
0.0435
CD284 (TLR4)
10
0.132
CD267 (TACI)
10
0.11
CD244 (2B4)
10 2
10 4
5
10 5
10
0.0435
10 3
0.107
10 3
CD70
0.388
CD57
10
5
10 5
0.0641
CD85g (ILT7)
10 2
10
0.0861
CD5
10
CD49c
2
10 2
10
10
10 2
10
2
10 2
10
CD314 (NKG2D)
0
0
0
0
0
0
0
0
0
0
0
0
0.0437
Ig light chain λ
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
2
3
10
4
10
4
2
10
3
10
4
2
3
10
4
10 2
10
10 4
10 4
10 3
10 4
43.4
10 3
5
5
5
10 5
10
10
10 5
10 5
10 5
0.0852
10 3
CD71
95.9
CD58
10
CD85h (ILT1)
10 2
10 2
10
99.8
CD49d
10
10 4
71.3
10 3
5
10 5
10
0.0662
CD29
10
3
100
10 4
CD16
10 3
0.088
CD6
10
CD42b
10 2
10
2
10 2
10
10
2
10
3
10
4
10
0
0
0
0
0
0
2
10
3
10
4
2
10
3
10
4
10
3
10
4
10 3
10 4
10 3
10 4
10
2
3
10
IgD
10
4
0.169
FcRL6
10 2
69.9
CCR10
10 2
0.308
CD340 (erbB2)
10
2
18.5
CD317
10
0.176
CD286 (TLR6)
10
0.044
5
5
5
5
10
5
10 5
10 5
10
10
10
CD268 (BAFF-R)
0
0.0221
CD245 (p220/240)
0
0
0
0
0
0
0
0
0
0.174
4
10 3
10
10 5
4
5
2
5
2
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
2
3
10
4
10
4
10
3
10
100
4
10 4
CD43
10 3
0.274
CD30
10
3
0.044
10 4
CD18
10 3
0.177
CD7
10
2
10
4
10 4
10 3
10 4
2
10
3
10
4
10
3
10
4
2
10
3
10
4
99.5
CD290
2
10
2
10
3
10
4
24.5
5
5
10
10
10
10
5
5
5
5
10 5
10 5
10 5
10
10
CD318 (CPCD1)
10
10
5
10 5
10
0.0219
CD270 (HVEM)
10
0.663
5
10 5
10
CD252 (OX40L)
10 2
10 4
0.568
10 3
0.28
10 3
CD73
100
CD85j (ILT2)
10 2
3
CD59
10
100
CD49e
2
10 2
10
10
10 2
10
2
10 2
10
10 2
10 3
10 4
10 5
10 2
10 3
10 4
10 5
0
10
2
3
10
IgM
10
4
10
0.0422
5
Galectin-9
0
0.463
CLEC12A
0
0.0646
CD344 (Frizzled-4)
0
0
0
0
0
0
0
0
0
0
0
0
0
0.192 10
10
3
4
10 5
4
5
2
5
4
5
2
3
4
5
2
2
4
2
3
4
5
2
5
3
0
10 2
10
10 4
10
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10
0
10
2
10 3
10 4
10 5
0
10
10 3
10 4
10
5
0
10 2
10 3
10
10
0
10 2
2
2
2
3
10
4
10
4
3
10
4
3
10
4
10 4
10 3
10 4
2
10
3
10
4
10
10
10
3
10
4
2
10
3
10
4
0.237
CD294
2
0.087
CD271
10
78.2
CD253 (Trail)
10 2
10 4
0.154
10 3
5
5
5
10 5
10
10
10 5
10
5
10 5
10
10
10
10
10
10
4
10
10 4
10 2
10 4
8.8
10 3
5
10 5
10 5
CLEC9A
10 3
0.0879
CD351
10 2
10
3
0
2
3
10
4
10
0.0844
10
IL-28RA
10
5
GARP (LRRC32)
0
0
0
2
0.0866
5
5
5
10 5
10 5
0.0211
10 3
CD74
0.172
CD61
10
29.3
CD49f
10
99.6
10 4
CD44
10 3
99.9
CD31
10
3
0.238
10 4
CD19
10 3
0.15
CD8a
10
CD85k (ILT3)
10 2
10 2
10
10
10 2
10
2
10 2
10
CD319 (CRACC)
0
0
0
0
0
0
0
0
0
0
0
0
0.0878
5
2
3
4
5
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
0
0
0
0
0
0
2
2
10
4
10
3
10
4
2
10
3
10
4
2
10
3
10
4
5
4
5
4
5
2
0
10
5
4
2
3
10
4
10 5
5
10 3
10 4
10 5
10 2
10 3
10 4
10 5
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
10 2
10 3
10 4
10
10
3
10
4
10
Ms IgG1, κ ITCL
0
2
0.191 5
10 5
TCR Vδ2
0
0
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10
10
10 5
0
Siglec-10
0
0.3
0
0
0
10
3
10
4
10
3
10
4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
4
0.172
CD201 (EPCR)
10
53.5
5
5
5
5
5
5
10
10
10 5
10 5
10
10
CD179b
10
10 4
0.108
10 3
CD163
10 2
10 4
0.108
10 3
CD150 (SLAM)
10 2
0.156
CD134
10
10
0.0672
CD115
10
2
0.262
10
10 5
0
10 2
10 3
10 4
1.02 10 5
10 2
10 4
10 3
10 4
0.111
10 3
Siglec-8
10 2
10
10
3
10
4
10
5
10 5
10 5
Ms IgG2a, κ ITCL
0
2
0.129
TCR Vγ9
0
0
0.0876
MSC and NPC (W4A5)
10 2
3
4
2
10 4
CD101 (BB27)
10
0
10 3
CD86
10 2
0.11
Integrin α9β1
0
10 2
10
10
10 5
10 4
MSC (W7C6)
10 3
10
0
40.1
Blank
10
2
10 2
10 2
10
5
10 3
10 4
10
10
0
0
0
0.0438
10 2
10 3
10 4
10 5
0
2
10 3
10 4
10
10
10
CD200 R
3
3
0
10
10
0
10
0
0
0
0
0
0
: FITC-A
10 3
10
10
0
2
10 3
10
10
10
4
10
5
2
3
4
5
0
10
10
10
10
10 5
2
10
10 5
2
3
4
5
0
10
10
10
10
0
2
3
4
10 2
3
10
10 5
5
5
10
10
10
10
5
0
10 2
10
10
0.191
CD179a
10
10 4
0.279
10 3
CD162
10 2
10 4
0.0849
10 3
CD148
10 2
10
10
0.0895
CD132
10
0.111
CD114
10
2
0.11
10
3
4
5
10 2
10
10
10
0
2
4
0
0
3
5
10 5
5
10 3
10 4
10
10
10 5
0
10
10
CD100
10
10 4
0.0888
10 3
0.11
Blank
10 2
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
10
10
10
5
0
10 2
10
10
10
0
10
10 3
10 4
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
0
0
0
0
0
0
2
2
3
4
5
2
3
4
4
2
10
4
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
10
2
10
3
10
4
0.252
10
5
5
10 5
CD180 (RP105)
10
10 4
5
5
5
10 5
10
0.0648
10 3
CD164
10 2
10 4
93.9
10 3
CD152
10 2
0.066
CD135
10
10
0.0883
CD116
10
2
0.247
10
10
2
10
3
10
4
10
5
0
0
0
10 3
10 4
10 2
10 4
10 5
10 5
10 3
10 4
10 5
0.0671
10 3
Siglec-9
10 2
0.087
MSCA-1 (MSC)
10 2
8.75
Integrin β5
0
99.2
CD202b (Tie2/Tek)
0
0
3
10 5
2
0
10
10 3
10 4
10
5
10
10
3
10
4
10
5
Ms IgG2b, κ ITCL
0
2
0.0898
TCR Vα24-Jα18
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
0.261
10 4
CD87
10 3
0.107
CD102
10
10 2
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
0
0
0
0
0
0
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
3
10
4
10
3
10
4
2
10
3
10
4
10
2
10 4
10
3
10
4
0.172
10 3
CD165
10 2
10 4
99.2
10 3
CD154
10 2
10
10
10
2
10
3
10
4
10
2
10
3
10
4
10
10 3
10 4
10 2
10 4
10 5
10 5
10 3
10 4
10 5
0.0443
10 3
SSEA-1
10 2
0.021
NKp80
10 2
10
10
3
10
4
10
5
Ms IgG3, κ ITCL
0
2
0.0672
TCR Vα7.2
0
0
0
0.111
Integrin β7
0
0.175
5
5
10
5
10 5
CD203c (E-NPP3)
0
0.213
5
5
5
10 5
0.0667
CD137 (4-1BB)
10
10
0.0877
CD117 (c-kit)
10
2
0.0658
10
10 5
CD181 (CXCR1)
0
10
0.109
10 4
CD88
10 3
0.238
CD103
10
10 2
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
0
0
0
2
10
3
0
10
4
10 4
CD89
10 3
10
2
10
3
10
4
0.462
CD104
10
10 2
10
10
5
5
10 5
0.0441
2
3
4
5
2
3
4
5
4
5
2
4
5
10
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
0.176
10
5
0
0
10
2
10
3
10
4
10
10
2
10
3
10
4
10
5
10
2
10
3
10
4
10
10 3
10 4
10 3
10 4
10
2
10
3
10
4
0.234
TCR α/β
10 2
10 4
10 5
10 5
10
5
10 5
0.0223
10 3
SSEA-3
10 2
3.51
Notch 1
10 2
5
Ms IgM, κ ITCL
0
0
0
0
0.0642
Jagged 2
0
0.125
CD205 (DEC-205)
0
0.107
5
10 5
CD182 (CXCR2)
0
10 4
10 5
0.0442
10 3
CD166
10 2
10 4
98.8
10 3
CD155 (PVR)
10 2
98.1
CD137L (4-1BB L)
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
CD119 (IFN-γRα chain)
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
10
3
10
4
2
10
3
10
4
10 2
0
5
10 5
10
10
5
5
2
10
3
10
4
2
10
3
10
4
2
3
10
4
10 3
10 4
95.1
LAP
10
10
2
10 2
10 4
10 4
10
3
10
4
5
5
5
10
5
10 5
10 5
10 5
10
10
0.0855
10 3
Tim-1
0.045
10 3
SSEA-4
10 2
98.8
Notch 2
10 2
10
2.33
CD206 (MMR)
10
10
0.0214
CD183
10
10 5
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
0.646
10 4
CD93
10 3
10
3
10
4
2
10
3
10
4
10
10
10
10 2
0
10 5
10
2
10
3
10
4
10
10
2
10
3
10
4
10
5
2
10
3
10
4
10 3
10 4
10 4
10
2
10 2
10 5
10 4
10
3
10
4
10
5
10 5
0.0441
10 3
Tim-3
5
10 5
10
0.0219
10 3
SSEA-5
10 2
0
Notch 3
10 2
0.131
LT-β R
10
Rat IgG2a, κ ITCL
0
0
0
0
0
81.5
CD207 (Langerin)
0
0.107
5
10 5
CD184 (CXCR4)
0
10 4
0.201
10 3
CD169
10 2
10 4
0.0649
10 3
0.177
5
5
5
10 5
CD140a
10
0.262
CD123
10
2
0.132
CD106
10
10 2
0.133
CD158a/h
0
0
0
0
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
Figure S2
Rat IgG1, κ ITCL
0
0
0
0
0
0
0
10 4
0.349
10 3
CD167a (DDR1)
10 2
10 4
0.26
10 3
97.8
CD138
10
2.64
CD122
10
2
10
10 5
0.0441
CD105
10
10 4
100
10 3
CD90 (Thy1)
10 2
100
CD156c (ADAM-10)
0
0
0
0
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
10
2
10 2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
54.4
10 4
CD94
10 3
0.264
10
10
10
4
2
10
3
10
4
10
10
10 2
10 4
2
10
3
10
4
10
2
10
10
3
10
4
10
0.0429
CD193 (CCR3)
10
5
5
10 5
10 5
5
5
10
2
10
3
10
4
10
5
10 3
10 4
10 3
10 4
10
2
10 2
10 4
10
3
10
4
10
5
10 5
10 5
10 5
0.0642
10 3
Tim-4
0.109
TCR γ/δ
10 2
0.133
Notch 4
10 2
Rat IgG2b, κ ITCL
0
0
0
0
0.352
Mac-2 (Galectin-3)
0
0.295
CD209 (DC-SIGN)
0
0
10 4
0.265
10 3
0.197
10 3
CD158b (NKAT2)
10 2
0.0668
CD140b
10
0.554
CD124
10
3
CD170 (Siglec-5)
0
0
0
0
2
0.223
5
10 5
CD107a (LAMP-1)
0
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
0
0
0
0
0
0
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
3
10
4
10
3
10
4
10
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10 3
10 4
10 2
10 3
10 4
0.132
NPC (57D2)
10 2
0.378
MAIR-II
10
0.255
CD210 (IL-10R)
10
0.243
CD195 (CCR5)
10
10 4
0.712
10 3
CD172a (SIRPα)
10 2
10 4
99.5
10 3
5
5
5
10 5
10
10 5
10 5
5
5
5
10
10
10
10 5
CD158d
10 2
0.09
CD141
10
92.8
CD126 (IL-6Rα)
10
2
0.086
10
10 5
10
2
10 2
10 4
10
3
10
4
0.107
10 3
TLT-2
10
5
10 5
Rat IgM, κ ITCL
0
0
0.152
TCR Vβ13.2
0
0
0
0
0
10
97.9
10 4
CD95
10 3
99.5
CD108
10
10 2
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
2
2
3
4
5
2
3
4
4
2
2
0
10
10 3
10 4
10
5
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
91.4
10 4
CD96
10 3
10
3
10
4
10
2
10
3
10
4
10 2
10
0
5
5
5
2
10
3
10
4
10
2
10
3
10
4
39.6
CD196
10
10 5
10
10
10
2
10
3
10
4
10
5
10 2
10 3
10 4
10 5
10 2
10 3
10 4
10 5
0
10 2
0
2
10
3
10
4
0.196
10 4
10
5
10 5
AH IgG, ITCL
10
10 3
TRA-1-60-R
0.0646
TCR Vβ23
0
0.132
Podoplanin
0
32
MICA/MICB
0
0.877
5
5
10 5
CD213a2
0
0
10 4
0.456
10 3
CD172b (SIRPβ)
10 2
10 4
0.0433
10 3
0.0441
CD143
10
1.07
CD127 (IL-7Rα)
10
2
10
10 5
0.0445
CD109
10
10 2
0.11
CD158e1 (NKB1)
0
0
0
0
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
0
0
0
0
0
0
2
2
3
4
5
2
3
4
4
2
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
3
10
4
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
4
0.432
CD197 (CCR7)
10
10 4
0.28
10 3
5
5
5
10 5
10
10
2
10
3
10
4
10 3
10 4
10 5
10 2
10 3
10 4
10 5
0.0221
Pre-BCR
10 2
10
0.0876
MSC (W3D5)
10
10 2
10 3
10 4
10 5
TRA-1-81
0
0.222
TCR Vβ8
0
0
0
11.5 5
5
5
10 5
CD172g (SIRPγ)
10 2
10 4
10
10
0.0856
10 3
CD158f
10 2
0.169
CD144
10
0.267
CD129 (IL-9R)
10
2
0.156
10
10 5
CD215 (IL-15Rα)
0
0
10
31.9
10 4
CD97
10 3
63.4
CD111
10
10 2
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
5
3
4
5
10
10
10
2
3
4
2
3
4
5
2
3
4
5
4
5
2
4
5
10
2
10 2
2
3
4
5
2
3
4
4
2
0
10
10 3
10
10 5
0
10 2
10
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
3
10
4
99.4
10 4
CD99
100 10 3
10
5
10 5
0
0
0
0
10
4
2
3
10
4
94.6
10
2
3
10
4
0.149
10
10
2
10
3
10
4
0.235
CD200 (OX2)
10
10 4
0.192 10 3
CD178 (Fas-L)
10 2
10 4
CD161
10 3
0.085
CD146
5
5
10
10
2
3
10
4
13.7
10
10 2
10
10 4
10 4
0.13 10 3
10 5
10 5
0.0663 10 3
PSMA
10 2
MSC (W5C5)
10
10 2
10 4
10 5
0.0855 10 3
TSLPR (TSLP-R)
0
TCR Vβ9
0
0
0
5
5
5
10 5
10 5
10
10
CD218a (IL-18Rα)
0
0
3
0.238
10
CD131
2
10 2
10
10
CD112 (Nectin-2)
0
0
: FITC-A
10 3
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10
10
10
10
0
10
5
0
10 2
10
10
10
0
2
10 3
10 4
10
10
10 2
10 3
10 5
10 4
10 5
10 2
0
10
10
3
10 2
10 3
10 3
5
2
10 5
2
3
4
5
10 5
10
10 4
10
2
10
3
5
2
10
5
10
3
10
4
2
5
2
2
3
4
5
2
10
3
10
4
10
5
2
10
3
10
4
10
2
10
3
10
10
4
10
0.149
CD352 (NTB-A)
10
10
10
10
HLA-A,B,C
10
0
0
5
10 2
4
3
0
10
10 2
3
2
3
4
5
0
10
10
10
10
0
2
10 4
3
2
5
10 5
10
90.2
5
5
10
10 3
10 4
10
10 4
CX3CR1
0
0
0.128
2
0
10
10 3
10 5
0
10
10
10
10
0
10
10 3
10 4
10
10
CD324 (E-Cadherin)
0
10
10 3
10 4
5
0.0636
10
2
10 4
0
2
CD298
10
5
0
10
10 3
10 4
10
10 5
0
100
10 5
0
10
10 3
10 4
10
4
CD273 (B7-DC)
10
0
3
4
10 2
10
10
0
3
4
10 2
10
10
6.22
2
3
0
10
10
10
10 5
0
10 3
2.19
CD254
10 2
10
10 5
0
10
10
10
0
3
Blank
10
4
2
2
0
10
10 5
10
5
10 3
10 4
10
4
0
0.13
10 5
0
10
10 3
10 4
10
10 4
CD79b
0
10 2
2
10 3
10
3
10
4
10 2
10 3
10 4
2
10
3
10
4
10
2
10
3
10
10
4
10
2
10
3
10
4
10
2
10
3
10
4
1.54
CD325
10
10
10
5
5
0
0
2
10
3
10
4
10
3
10
4
HLA-A2
10
2
10
10
0.214
CXCR7
10
0.958
5
5
CD354 (TREM-1)
0
0
1.69
5
0.0643
CD274 (B7-H1)
10
CD300e (IREM-2)
0
0
20.2
5
10 5
0.214
5
10 5
10
0.472
10 4
CD80
CD220
10
10 2
CD255 (TWEAK)
0
0
0
0
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0.215
10 4
10 2
10 3
10 5
10 3
0
0
10 3
CD62L
10 2
10 5
0.0853
10 4
CD51
10 3
0
10
2
10 3
10 4
10 5
10 4
0
10 2
99.8
0
10 5
10 2
1.13
10 5
10 2
10 3
10 4
10 5
CD62E
10 4
10
5
10 4
10 3
0
10 3
10
4
10 2
10 3
10 4
10
5
10 4
0
10 2
10
3
10 5
0.128
10 4
CD33
10 3
CD45RA
10
2
10 2
10 5
0
0
0
0
10 2
10 3
10 5
10 2
0
10 5
0.106
10 4
CD50 (ICAM-3)
0
0
10
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
0.0647
10 5
2
10 3
10 4
10
5
10 5
CD45
10
4
0
2
0
10 3
10 4
10
5
10 2
0.0432
10 5
10 2
10 3
10 4
10
5
10 4
CD32
10 3
0
0
10 2
10 3
10 4
10 5
0.238
10 4
CD21
10 3
10 4
0
0.173
10 2
10 5
0
0
10 2
10 5
10 2
10 3
10 4
10 5
CD20
10 3
10 4
10 3
10 5
10 3
0
10 2
10 5
0.106
10 4
CD10
10 3
10 4
0
0
10 2
0
10 2
10 3
10 5
10 2
0
0
39.1
10 5
10 4
10 3
10 2
10 3
10 4
10 5
10 4
CD9
10 3
10 4
CD1a
10 3
10 4
0
10 2
10 2
10 5
10 2
0
0
0
10 2
10 5
0
99.2
10 5
10 2
10 3
10 4
10 5
Blank
10 4
0
0
10 4
10 3
10 5
0.105
10 4
10 3
10 5
0
0.0855
10 2
10 3
10 4
10 5
10 2
10 4
10 3
10
3
10
4
0.15
10 4
CD34
10 3
10 5
10 5
10
10 2
10 3
10 5
10 2
10 3
10 5
10
2
10 2
10 3
10
3
10
4
2.37
10 4
CD81
10
10 3
10 4
2
10
3
10
4
2
10
3
10
4
10
10
2
10
3
10
4
10
0.424
CD300F
10
10
5
5
5
10 5
0.255
CD275 (B7-H2)
10
3.65
CD257 (BAFF)
10 2
10
2
10
3
10
4
10
10
2
10
3
10
4
10
0
10
3
10
4
10
HLA-DQ
10
2
5
5
5
0.0859
δ-Opioid Receptor
0
0.148
CD355 (CRTAM)
0
0.278
CD326 (Ep-CAM)
0
0
0
0
0.856
5
10 5
CD221 (IGF-1R)
0
0
99.9
10 4
CD62P (P-selectin)
0
0.17
10 4
97.1
5
10 5
0.303
10 4
CD22
10 3
0.15
10 4
CD45RB
10
2
10 2
10 2
10 5
0.0845
CD1b
10 3
CD11a
10 2
10 2
CD51/61
0
0
0
0
0
0
0.169
10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 4
10 3
10
2
10 2
10 2
10
3
10 5
10
4
10
10
2
10 2
10 2
10 2
10 3
10
3
10
4
10
5
10 5
10 5
10 5
0.192
10 4
CD82
17.7
10 4
CD63
10 3
99.6
10 4
CD52
10 3
10 3
10 4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
10
5
5
10 5
10
4
10
5
0.0211
CD301
10
4.74
CD276
10
100
CD258 (LIGHT)
10 2
0
0
0
2
10
3
10
4
2
2
3
10
4
10
3
10
10
4
10
0.043
DLL1
10
HLA-DR
10
10
10
5
5
5
0.0425
CD357 (GITR)
10
0.989
CD328 (Siglec-7)
0
0
0
0
0.194
CD226 (DNAM-1)
0
0
0
0
0.107
5
10 5
0.127
10 4
0.0649
10 4
CD35
10 3
10 5
0.0853
10 4
CD23
10 3
10 5
0.277
CD1c
10 3
CD11b
10 2
10 2
CD45RO
0
0
0
0
0
0.105
10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 2
10 2
10 4
10 3
10 5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
10
4
10
3
10
4
10 3
10 4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
4
2
2
3
10
4
10
3
10
10
10
4
10
0.215
DLL4
10
HLA-E
10
10
1.71
CD360 (IL-21R)
10
21.8
CD334 (FGFR4)
10
10
0.107
CD303
10
10
5
5
5
5
5
5
10 5
0.172
CD277
10
17.6
CD261 (DR4)
10 2
0.194
10
5
10 5
0.107
10 4
CD83
10 3
10 5
0.732
10 4
CD64
10 3
10 5
10
5
10 5
0.234
10 4
CD53
10 3
0.3
CD46
10
3
45.5
10 4
CD36
10 3
10 5
10 5
0.259
10 4
CD24
10 3
CD229 (Ly-9)
10
10 2
10 2
10 2
10
2
10 2
10 2
46.1
10 4
0.875
CD1d
10 3
CD11b (activated)
0
0
0.105
10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
10 4
10 3
10 2
10 2
10
2
10 2
10 2
10
4
99.8
10 4
10 3
10 5
10
0
0
0
0
0
0
0
2
10 3
10
3
10
4
10 3
10 4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
10
2
10
3
10
4
99.9
10
CD335 (NKp46)
10
10
10
0.106
CD304
10
88
CD278 (ICOS)
10
5
5
5
5
10 5
0.107
CD262 (DR5)
10 2
81.9
10
5
10 5
0.341
10 4
CD84
CD231 (TALLA)
10
10 2
10 5
0.323
10 4
5
10 5
0.215
10 4
CD54
10 3
11.4
CD47
10
3
10 5
0.195
10 4
CD38
10 3
10 5
0.129
10 4
CD25
10 3
10 5
0.128
CD2
10 3
CD11c
10 2
10 2
CD66a/c/e
0
0
0
0
0
0
0
0.0629
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
10
2
10
3
10
4
10
0
10
3
10
4
HLA-G
10
2
10
0.193
DR3 (TRAMP)
0
0.17
5
5
β2-microglobulin
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
10 4
10
4
10 3
10 3
10
2
10
3
10
4
10
0
0
0
0
0
0
0
10 3
10 4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
10
10
10
3
10
4
10
3
10
4
99.2
BTLA
10
2
10
3
10
10
10
4
10
0.577
EGFR
2
2
0.234
CD336 (NKp44)
10
10
0.214
CD307
10
10
5
5
5
5
5
5
10 5
0.107
CD279 (PD-1)
10
0.15
CD263 (DcR1)
10 2
0.493
5
10 5
10 5
0.214
10 4
0.13
10 4
CD85a (ILT5)
10 2
10 5
10
5
10 5
0.128
10 4
CD55
10 3
86.4
CD48
10
3
0.28
10 4
CD39
10 3
10 5
10 5
10 5
0.108
10 4
CD26
10 3
60.4
10 4
CD13
10 3
100
CD3
10 3
CD66b
10 2
10 2
10
2
10 2
10 2
10 2
10 2
CD235ab
0
0
0
0
0
0
0
0
0
0.0845
IFN-γ R β chain
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
10 4
10
3
10
4
87.2
10 4
CD40
10 3
10 3
10 3
2
10
3
10
4
10 3
10 4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
4
10
10
10
2
2
3
10
4
10
3
10
10
4
10
0.215
C3AR
10
0.256
CD337 (NKp30)
10
0.214
CD307d (FcRL4)
10
10
5
5
5
5
5
0.0427
CD282 (TLR2)
10
10 5
0.257
CD266 (Fn14)
10 2
40.3
10
5
10 5
0.238
10 4
CD243
10
10 5
0.215
10 4
CD69
10 3
10 5
0.0631
10 4
CD85d (ILT4)
10 2
10 2
10
5
10 5
0.769
CD56 (NCAM)
10 2
10 5
0.581
10 4
CD27
10 3
10 5
0.172
10 4
CD14
10 3
10 5
0.0637
CD4
10 3
CD49a
10
2
10 2
10 2
10 2
10 2
0.105
3
0
10
2
10
3
10
4
10
5
0.0646
Ig light chain κ
0
10 2
10
10 4
10 5
erbB3/HER-3
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
10 4
10
3
10
4
89.1
10 4
CD41
10 3
10 3
2
10
3
10
4
10 3
10 4
2
10
3
10
4
10
2
10
3
10
10
4
10
0.259
CD284 (TLR4)
10
0
0
0
0
2
10
3
10
4
10
2
2
3
10
4
10
3
10
2
10
3
10
10
4
10
4
10
0.107
10
0.234
C5L2
10
FcεRlα
10
10
0.725
CD338 (ABCG2)
10
0.151
5
5
5
5
5
5
10 5
0.279
CD267 (TACI)
10 2
0.0857
10
5
10 5
0.108
10 4
CD244 (2B4)
10
10 5
10 5
0.0214
10 4
CD70
10 3
18.4
10 4
CD57
10 3
10
5
10 5
0.0857
CD85g (ILT7)
10 2
10 2
10 2
10 5
0.108
10 4
CD28
10 3
10 5
0.646
10 4
CD15
10 3
10 5
0.107
CD5
10 3
CD49c
10
2
10 2
10 2
10 2
10 2
CD314 (NKG2D)
0
0
0
0
0
0
0
0
0
0
0
0
0.0628
Ig light chain λ
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 4
10 3
10
3
10
4
90.1
10 4
10 3
10
2
10
3
10 5
10 5
10
10
4
94.8
10 4
5
10 5
10
5
10 5
0.129
10 4
CD71
10 3
89.7
10 4
CD58
10 3
CD85h (ILT1)
10 2
10 2
10 2
99.7
CD49d
10
2
10 5
10 5
0.172
10 4
CD29
10 3
100
10 4
CD16
10 3
10 5
0.0851
CD6
10 3
CD42b
10 2
10 2
10 2
10 2
10 2
10 3
10 4
10 5
0
0
0
0
0
0
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
IgD
10
3
10
4
10
0.192
FcRL6
10
10
10
10
10
0.257
CCR10
10
0.47
CD340 (erbB2)
10
38.2
CD317
10
2.63
CD286 (TLR6)
10
0.151
5
5
5
5
5
5
CD268 (BAFF-R)
0
0.171
CD245 (p220/240)
0
0
0
0
0
0
0
0
0
0.147
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
10 4
10
3
10
4
100
10 4
CD43
10 3
0.28
10 4
CD30
10 3
2
10
3
10
4
1.95
10 4
10
10 3
10 4
2
10
3
10
4
10
2
10
3
10
4
25.4
CD290
10
10
10
10
2
10
3
10
4
10
5
10
2
10
3
10
4
10
5
10
2
10
3
10
4
10
5
0
10
2
10
IgM
10
3
4
10
5
0.0214
Galectin-9
0
0.469
CLEC12A
0
0.106
CD344 (Frizzled-4)
0
1.2
5
5
10 5
0.235
CD270 (HVEM)
10 2
2.85
5
10 5
CD252 (OX40L)
10
10 3
10 5
10 5
10
5
10 5
10 5
0.214
10 4
CD73
10 3
100
10 4
CD59
10 3
100
CD85j (ILT2)
10 2
10 2
10 2
10 5
0.043
10 4
CD18
10 3
10 5
0.0639
CD7
10 3
CD49e
10
2
10 2
10 2
10 2
10 2
CD318 (CPCD1)
0
0
0
0
0
0
0
0
0
0
0
0
0.189 10 3
10 4
10 5
5
2
2
3
4
3
4
5
2
2
5
2
2
3
4
5
3
0
10 2
10
10 4
10 5
0
10
10
10
10
0
10
10 3
10 4
10
0
10
10 3
10 4
10 5
0
10
10 3
10 4
10
0
10 2
10
10
10 5
0
10
10
10
10 5
0
10
10 3
10 4
10
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
2
10 3
10 4
10 5
0
10 2
10 3
10 4
10
5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10 2
10 4
10
3
10
4
78.5
10 4
CD44
10 3
100
10 4
CD31
10 3
10 3
2
10
3
10
4
10 3
10 4
2
10
3
10
4
10
2
10
3
10
10
4
10
0.281
CD294
10
5
5
5
10 5
10
10 5
10 5
0.281
CD271
10 2
42.8
CD253 (Trail)
10
0.15
10 4
0.15
10 4
CD74
10 3
CD85k (ILT3)
10 2
10 2
10 5
10
5
10 5
10 5
0.237
10 4
45.4
CD61
10 3
10 5
0.992
10 4
CD19
10 3
10 5
0.0854
CD8a
10 3
CD49f
10 2
10
2
10 2
10 2
10 2
10 2
10
3
10
4
2
10
3
10
10
4
10
0.129
CD351
2
10
2
10
3
10
4
65.1 10
CLEC9A
10
10
5
5
5
0
10 3
10 4
10 5
0.235
IL-28RA
10 2
GARP (LRRC32)
0
0
0
0.15
CD319 (CRACC)
0
0
0
0
0
0
0
0
0
0
0
0
0.0426 2
3
4
5
3
10
4
2
3
0
10
3
5
4
3
10
4
5
10
10
3
2
3
4
5
2
10
3
10
4
10 2
10 3
4
2
3
4
5
4
5
2
2
3
4
5
2
2
3
10
4
10
3
10
4
11.8
Blank
10
10
2
10
3
10
10
10
4
10
0.129
MSC (W7C6)
10
10
10 2
10 3
10 4
10
3
10
4
10
5
Ms IgG1, κ ITCL
2
3
10
4
2
10
3
10
4
2
10
3
10
4
10
3
10
4
2
10
3
10
4
10 2
10 3
10 3
10
2
10
3
10
4
5.39
10 4
99.1
10 4
CD201 (EPCR)
10 2
10
5
5
5
5
10
5
10 5
10 5
0.107
CD163
10
10
0.217
CD150 (SLAM)
10
2
10
0.273
CD134
10
10
0.108
CD115
10
5
10 5
0.296
10 4
CD101 (BB27)
10 3
10
0.129
CD86
10
CD179b
0
2
10 2
10
0
10
2
10
3
10
4
1.84 10
5
4
5
2
2
3
4
5
0
10
10
10
10
0
10
10 3
10
10
2
3
0
10
10
10
10
2
10
3
10
4
10 2
10 3
10
10 4
10
2
10
3
10
4
10
0.107 5
10 5
Ms IgG2a, κ ITCL
0
5
0.0863
Siglec-8
TCR Vγ9
0
0
0.129
MSC and NPC (W4A5)
4
2
3
0
10
2
3
4
5
0
10
10
10
10
0
0
0
0
0
0
0
0
0.128
Integrin α9β1
0
10 2
4
10
10
10
0.152
4
10 3
10
10 5
10 5
0
5
5
5
10 5
TCR Vδ2
0
0.153
Siglec-10
0
0
0
0.0866
10 5
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
10 4
0
0
CD200 R
4
10 2
10 3
10
10 5
0.213
10 2
10 3
10
4
10 5
10 5
10 4
CD179a
10 3
0 10 2
10 2
0
10 3
10 4
5
10 2
10 3
0.279
10
10 4
CD162
10
2
3
4
5
0
10
10
10
10
10 5
0
0
0.194
10
5
10 5
0
10
10
10
10
CD148
10
4
0 2
4
10 3
10
10
5
0
0.281
10
10 2
0
10
10 2
10 3
10
10
5
2
CD132
10
2
0
10
10 3
10 4
2
0
10
0.302
10 4
10 3
10 5
10
10 5
0
4
CD114
10
0
0 2
10 2
10
10 4
10 3
10 5
10 2
10 3
0.171
10 5
10 4
CD100
10 4
2
3
0
10
10
10
10 5
0
10
10
10
10 3
5
4
10
0.152
Blank
10
4
0
2
10 2
10
2
3
4
5
0
10
10
10
10
10 5
0
0
0.0431
10 5
0
10
10
10
10
2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
3
10
4
2
10
3
10
4
2
10
3
10
4
10
3
10
4
2
10
3
10
4
10 2
10 3
10
10
10 2
10 3
10 5
10 5
5
5
5
5
10
2
10
3
10
4
10
5
0
0
0
2
10
3
10
4
2
10
3
10
4
10 2
10 3
10
10 4
5
5
10 5
0.108
Siglec-9
10
10
0.0853
MSCA-1 (MSC)
10
32.1
Integrin β5
0
99.1
10 4
3.31
10 4
0.147
CD164
10
99.7
CD152
10
2
10
0.126
CD135
10
10
0.087
CD116
10
5
10 5
10
0.451
10 4
CD102
10 3
4.8
CD87
10
CD180 (RP105)
0
2
10 2
10
CD202b (Tie2/Tek)
0
0
0
0
0
0
0
0.15
2
3
4
0
10
10
10
10 5
10
2
10
3
10
4
10
5
Ms IgG2b, κ ITCL
0
0.106
TCR Vα24-Jα18
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
0
2
3
10
4
10 3
2
10
3
10
4
2
10
3
10
4
10
3
10
4
2
10
3
10
4
99.4
10 2
10 3
10 2
10 3
10
2
10
3
10
4
10
5
2
10
3
10
4
2
10
3
10
4
10 2
10 3
10
10 4
5
5
10 5
0.278
SSEA-1
10
10
0.149
NKp80
10
10
2
10
3
10
4
10
5
Ms IgG3, κ ITCL
0
0.15
TCR Vα7.2
0
0
0
0.171
Integrin β7
0
10 5
0.128
10 4
CD203c (E-NPP3)
0
0.322
10 4
5
5
5
5
10 5
10
10
0.211
CD165
10
10
0.064
CD154
10
2
10
0.259
CD137 (4-1BB)
10
5
10 5
0.107
10 4
CD117 (c-kit)
10
10
0.172
CD88
10
CD103
10 2
10
CD181 (CXCR1)
0
0
0
0
0
0
0.191 2
3
4
5
2
3
4
0
0
0
2
3
10
4
10 3
10
2
10
3
10
10
4
5.71
10 4
5
10
5
10 5
0.107
CD89
10
CD104
10 2
10
0.086
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
10
2
10
3
10
4
10
5
10
10
4
2
10
3
10
4
10 2
10 3
10
10
10 2
10 3
10 5
10
2
10
3
10
4
10
2
10
3
10
4
2
10
3
10
4
10 3
10 4
10
2
10
3
10
4
5
5
10
5
10 5
10
0.471
TCR α/β
10 2
0.17
SSEA-3
10
10
0.279
Notch 1
10
5
Ms IgM, κ ITCL
0
0
0
0
0.149
Jagged 2
0
0.195
10 4
10 5
5
5
CD205 (DEC-205)
0
0.15
10 4
0.192
CD166
10
99.6
CD155 (PVR)
10
3
CD182 (CXCR2)
0
0
0
2
99.7
CD137L (4-1BB L)
0
0.429
CD119 (IFN-γRα chain)
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
4
98.2
CD138
10
1.13
CD122
10
5
10
10
10
5
5
5
10 5
10
0.043
10 4
CD105
10 3
100
CD90 (Thy1)
2
10 2
10
2
10
3
10
4
10 3
10 3
2
2
3
10
10
3
4
10
4
97.3
LAP
10
2
10
3
10
4
10
2
10 2
10 4
10
3
10
4
5
5
5
10
5
10 5
0.213
Tim-1
10 3
10
10
10
10 5
0.235
SSEA-4
10
63.1
Notch 2
10
10
32.7
10 4
CD206 (MMR)
10 2
5
10 5
10
0.151
10 4
CD183
10 2
2.51
CD167a (DDR1)
10
2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
3
10
4
10 3
2
10
3
10
4
2
10
3
10
4
73.5 10
10
10
2
10
3
10
4
10
0.216
CD140a
10
10 5
5
5
5
5
0.0432
10 4
CD123
10
10
0.621
CD93
10
CD106
10 2
10
0.195
10
3
10
4
10 2
10 3
10 2
10 3
10 5
10 5
5
2
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
2
10 2
10 4
10
3
5
5
5
10
4
10
5
10 5
0.235
Tim-3
10 3
10
10
10
0.0859
SSEA-5
10
0.28
Notch 3
10
5.2
LT-β R
10
Rat IgG2a, κ ITCL
0
0
0
0
0
89.5
10 4
0.28
10 4
0.278
10
CD207 (Langerin)
0
2
CD169
10
CD184 (CXCR4)
0
0
0.192
CD158a/h
0
0
0
0
0
Figure S3
Rat IgG1, κ ITCL
0
0
0
0
0
0
0
0
0.62
CD156c (ADAM-10)
0
0
0
0
0
94.8 2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
10 2
10
3
10
4
10 3
2
10
3
10
4
2
10
3
10
4
10
10
10
3
10
4
10
10
2
10
3
10
4
10
0.279
CD158b (NKAT2)
10
2
0.281
CD140b
10
7
CD124
10
5
5
5
5
10 3
10 2
10 3
10 5
10
2
10
3
10
4
10
5
10 5
2
10
3
10
4
2
10
3
10
4
10
2
10 2
10 4
10
3
10
4
0.15
Tim-4
10 3
0.17
TCR γ/δ
10
0.3
Notch 4
10
5
5
10
5
10 5
10
10
Rat IgG2b, κ ITCL
0
0
0
0
0.687
Mac-2 (Galectin-3)
0
0.28
10 4
0.0858
10 4
CD193 (CCR3)
10 2
CD209 (DC-SIGN)
0
0
0.322
CD170 (Siglec-5)
0
0
0
0
5
10 5
10
0.108
10 4
88
CD94
10
CD107a (LAMP-1)
0
0
37.2 2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
3
10
4
10 3
2
10
3
10
4
10
10
3
10
4
2
10
3
10
4
0.26
CD141
2
10
10
2
10
3
10
4
10
10 3
10 3
2
10
3
10
4
2
10
3
10
4
10
2
10
3
10
10
10
4
10
0.323
NPC (57D2)
10
10 5
5
5
5
5
5
5
5
10 5
0.384
MAIR-II
10
10.6
10 4
CD210 (IL-10R)
10 2
0.171
10 4
CD195 (CCR5)
10 2
0.669
CD172a (SIRPα)
10
99.5
CD158d
10
10
28.5
CD126 (IL-6Rα)
10
5
10 5
10
0.151
10 4
97.9
CD95
10
CD108
10 2
10
10
2
10 2
10 4
10
3
10
4
0.3
TLT-2
10 3
10
5
10 5
Rat IgM, κ ITCL
0
0
0.106
TCR Vβ13.2
0
0
0
0
0
0
0
0
0
0
0
99.6 2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
2
3
4
0
10
10
10
10 5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
3
10
4
2
10
3
10
4
10
10
10
10
3
10
4
2
10
3
10
10
4
10
0.215
CD143
2
39.8
CD127 (IL-7Rα)
10
5
5
5
2
10
3
10
4
10
10 3
10 2
10 3
10 5
10
2
10
3
10
4
5
10
5
10 5
0.644
10 4
23.6
10 4
CD196
10 2
0.432
CD172b (SIRPβ)
10
0.13
10
2
10
3
10
4
10
5
2
10 2
10
3
10 3
10
4
10 4
0.15
10
5
10 5
10
0
2
10
3
10
4
10
0.302
AH IgG, ITCL
10
5
TRA-1-60-R
0
0
0.236
Podoplanin
0
30.7
MICA/MICB
0
5
10 5
10
0.151
10 4
CD109
10 3
88.7
CD96
10
CD213a2
0
2
10 2
10
CD158e1 (NKB1)
0
0
0
0
0
0
0
0.13 2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
3
10
4
10 3
10
3
10
4
10
3
10
4
2
10
3
10
4
2
10
3
10
4
10
0.259
10
10 3
10 2
10 3
2
10
3
10
4
2
10
3
10
4
10
10
2
10
3
10
4
10
0.259
Pre-BCR
10
10
10 2
10 3
10 4
10 5
0.191
5
5
TRA-1-81
0
5
0.0644
MSC (W3D5)
10
10 5
TCR Vβ8
0
0
0
90.6
10 4
5
5
5
5
10 5
0.838
10 4
CD197 (CCR7)
10 2
0.541
CD172g (SIRPγ)
10
10
0.259
CD144
2
10
0.562
CD129 (IL-9R)
2
CD158f
10
10
10
5
10 5
10
0.217
10 4
24.6
CD97
10
CD111
10 2
10
CD215 (IL-15Rα)
0
0
0
0
0
0
0
0
27.4 2
3
4
5
2
3
4
2
4
2
3
4
5
4
4
2
3
4
5
4
5
2
2
3
4
5
0
10
10
10
10
0
10
10 3
10
10
0
10
10
10
10
0
10 2
10 3
10
10 5
0
10 2
10 3
10
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10
0
10 2
10 3
10
10
5
0
10
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
0
10
10
10
10 5
0
10
10
10
10
2
10 2
10
10
3
10
4
100
10 3
5
10 5
10
3
10
4
10
3
10
4
80.3
2
10
3
10
4
10 3
10 2
10 3
10
10 5
2
10
3
10
4
90.9
10
10
2
2
3
10
3
10
10
4
10
4
10
10 2
10 3
10 4
10 5
0.278
5
TSLPR (TSLP-R)
0
5
5
0.0859
10
0.171
PSMA
10
MSC (W5C5)
10
TCR Vβ9
0
0
0
10 4
13
5
10 5
0.691 10 4
5
5
10 5
0.303
10 4
CD200 (OX2)
10 2
10
0.152
CD161
10 3
CD146
2
10
0.218
CD178 (Fas-L)
10
10
CD131
2
10 2
10
10
CD218a (IL-18Rα)
0
0
0
0
0
0
10 4
99.4
CD99
CD112 (Nectin-2)
0
0
A
B
EGFR DAPI
PAX7 Laminin
Merged
EGFR
PDGFRα
Merged
C
D
E
CD318 PAX7 Laminin DAPI
CD318 CD56 Laminin DAPI
CD318 CD56 DAPI Figure S4
CD56(+) CD56(-)
A
SSC
62.3 6.46
20K
100
80
80
% of Max
40K
100
% of Max
60K
CD56(+) CD56(-)
60
40
20
0 10 1
10 2
10 3
10 4
10 5
0 10 0
10 1
10 2
CD56
B
40
20
0 10 0
60
10 3
10 4
10 5
10 0
10 1
CD82
10 2
10 3
10 4
10 5
CD318
10 5
E 10 5
10 4
10 4
CD201
CD82
6.78 10 3
10 2
69.3
10
2
10
1
10
1
10
0
10
0
10 0
10 1
10 2
10 3
10 4
CD56(+)CD82(+)
69.5
5.97 10 0
10 5
10 1
10 2
10 3
F
CD56(-)CD82(-) 0
67.9±6.1
0
PAX7
MYOD1
Perilipin DAPI
81.1±7.0
PDGFRα(+)CD201(+)
DAPI
Figure S5
MyHC DAPI
D
CD56(+)CD82(+)
10 4
10 5
PDGFRα
CD56
C
10 3
CD56(-)CD82(-)
PDGFRα(-)CD201(-)
A
C
10
2
CD82
10
3
10
0
4
E
PAX7/GAPDH
Relative quantity
0.6 0.4 0.2 0
1 0.8 0.6 0.4 0.2
1 0.8 0.6 0.4 0.2
0
1.2 1 0.8 0.6 0.4 0.2
0
0
F 13.4
29.2
CD82(+)CD56(-)
CD82(+)CD56(+)
10 4
0
CD82(-)CD56(-)
CD82(-)CD56(+)
Negative control
1
D )C
)C MyHC
10 2 0 99.8 0
0.102 10 2
10 3
10 4
10 5
Figure S6
82 D C
(+ 82 D
C
82 C
D
10 3
56
56
56 D
4
(+
10
(+ )
0
0
D
0.0637
2
(-)
10 5
3
C
10
(-)
10
5 4
(-)
CD56
5
56
10
4
D
10
3
82
0
23.9 2
6
D
33.4
7
C
2
8
C
10
9
(+ )
3
MyHC+ area (x106 μm2)
10
10
(-)
5
10
CD82
MYOD1/GAPDH
1.2
+ CD 82 -
10
1
0.2
+ CD 8 Un 2so rt e d
10
0
0.4
1 0.8
+ CD 8 Un 2so rt e d
0
0.6
CD 82
10
Relative quantity
10 1
1 0.8
CD 82
Relative quantity
CD318
CD82(-) CD82(+)
MYOD1/GAPDH 1.2
CD 82
2
MYF5/GAPDH 1.2
Relative quantity
10
PAX7/GAPDH 1.2
+ CD 82 -
3
CD 82
10
D
Relative quantity
4
+ CD 8 Un 2so rt e d
10
CD 82
B
Supplemental Figure Legends Figure S1 (Related to Figure 1). Optimization of coating substrates and growth factors for the expansion of human muscle-derived progenitors. (A) Muscle-derived progenitors were cultured for 3 d on the indicated coating substrates. Collagen I, collagen IV, fibronectin, and laminin were used as coating substrates. Number of cells per fields was quantified and expressed as means ± s.d., n=15 fields from three independent preparations. *P
