Cell & Tissue Biology Research

ISSN: 1309 2375

Official Publication of the Turkish Society of Histology and Embryology

Cell & Tissue Biology Research Turkish Histology and Embryology Association

www.ctbiol.com

Volume 6 / 2018 Supplement Special Issue includes abstracts of the 14th National Histology and Embryology Congress 10-13 May 2018 Hotel Su Antalya / Türkiye

Official Publication of the Turkish Histology and Embryology Association About the Journal Cell and Tissue Biology Research is an official journal of the Turkish Histology and Embryology Association. It is an online journal publishing research articles after full peer review. All articles are published, without barriers to access, immediately upon acceptance. One volume is published every year. Each volume consists of 4 numbers published quarterly online. Aims and Scope Cell and Tissue Biology Research is a peer-reviewed journal that publishes original research on all aspects of anatomy, histology, cell biology and fine structure of tissues and organs on light and electron microscopical level, neuroanatomy, and morphological techniques, as well as developmental biology, focusing on morphogenesis—the study of the emergence of form during embryogenesis, mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, and genetic levels. Published manuscript styles include original research articles, review articles, technical notes, case reports, short communications, and letters to the editor. Novel features include: full peer review, high quality of reproduction, rapid publication, no page charges, wide readership, and fast track submission. Indexing and archiving Cell and Tissue Biology Research aims to be indexed in all major national and international databases in the neer future. Copyright All rights reserved. Apart from any relaxations permitted under national copyright laws, no part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without the prior written permission of the copyright owners. Permission is not required to copy summaries of papers or of articles on the condition that a full reference to the source is given. Multiple copying of the contents without permission is always illegal. Contact Information Queries about content, submissions, or the review process should be directed to the Turkish Histology and Embryology Association. If you have proposals or feedback related to this journal or this field of science, please do not hesitate to contact the Managing Editor Dr.A. Cevik Tufan, E-mail: [email protected]. Readership Mainly consists of (but not limited to) histologists, microscopists, developmental biologists, embryologists, molecular and cellular biologists, biochemists, geneticists, neurobiologists, anatomists, pathologists, physiologists. Instructıons to Authors Cell and Tissue Biology Research publishes original research articles, review articles, technical notes, case reports, short communications, and letters to the editor within the scope of the journal. The content of the Cell and Tissue Biology Research is determined by the Editors. The manuscript which is submitted to the journal must not contain previously published material or material under consideration for publication elsewhere, except for a preliminary report in abstract format. Accepted manuscripts become the property of Cell and Tissue Biology and may not be republished. The decision on acceptance of manuscripts for publication in Cell and Tissue Biology Research will be made on the basis of a peer review system. Responsibilities of the Authors By submitting a manuscript for publication, each author acknowledges having made a substantial contribution in the conception and design of the study, the analysis and interpretation of the results, and the writing of the paper, and has approved the final submitted version of the paper. Each author thus also acknowledges responsibility for the integrity of the manuscript, assures the originality of the manuscript, and guarantees that duplicate or redundant publications or submissions have not occurred. The Editors reserve the right to request the original data obtained in the investigation. Authors are responsible for all statements made in the text.

Manuscript Submission Manuscripts and illustrations should be submitted in English, online to the editorial office. Authors should retain their own copy, as the Editor cannot accept responsibility for damage or loss of manuscripts. The text document must be saved as Word or RTF format. Tables must be included in the text document. Figures must be saved in the formats and at the resolution indicated below (illustrations section). The manuscript should be typed double-spaced throughout on one side of A4 paper with at least a 2.5 cm margin on all sides. Do not divide words at the end of lines. Pages should be numbered consecutively in Arabic numerals, beginning with the title page. Prepare a cover letter and a copyright transfer form signed by all authors. Organize the manuscript as follows: title page, abstract (including key words at the end), introduction, material and methods, results, discussion (including the conclusions), acknowledgments, references, figure legends, and tables. Keep acronyms and abbreviations to a minimum. When an abbreviation is used, define it at first mention and follow with the abbreviation in parentheses. Categories of Submission Review Articles Cell and Tissue Biology Research publishes review articles on the basis of invitation by the editor(s). However, author(s) is free to suggest topics and manuscripts for publication in the journal as a rewiev article as well. The author(s) is absolutely free to design the paper. There is no limitation in the page count in this category. References, figures, and legends follow the guidelines described below under ‘Original Articles.’ The Abstract section is needed. Original Articles Title Page. The following information should appear: title of article; authors’ name, and last name; affiliations with complite addresses. Identify the corresponding author and provide full mailing address, phone and fax numbers, and e-mail address. Also provide a short running title (no more than 40 spaces). Abstract. The abstract is limited to 400 words, and should describe the essential aspects of the investigation. In the first sentence state the background; in the second sentence state your specific purpose or hypothesis; in the third, fourth and fifth sentences summarize methods, results and conclusion. No references should be cited. For indexing purposes, a small number of "key words" (no more than 5) must be supplied. Introduction. Include brief background information on what has been done in the past in this area and the importance of your investigation. End with a statement of the purpose or hypothesis of the study. Material and Methods. This section may be divided into subsections if it facilitates reading the paper. The research design, subjects, material used, and statistical methods should be included. Do not mix results and discussion into this section. Do not include manufacturer’s names unless the specific product is important to the procedures performed, in which case the city and state or country of the manufacturer should also be given. Indicate that informed consent has been obtained from patients who participated in clinical investigations. In animal experimentation, acknowledge that ethical guidelines were followed. When appropriate, indicate that approval was obtained from the institution’s review board. Results. This section may be divided into subsections if it facilitates reading the paper. All results based on methods must be included. If tables and graphic material will ease the understanding of the results, include them. Cite figures to illustrate the findings of the study. Discussion. Start with limited background information and then discuss the results of the investigation in light of what has been published in the past, the limitations of your study, and potential directions for future research. In appropriate place, cite figures and graphs. Following this information, summarize the major findings of the study and their clinical usefulness. This paragraph should address the hypothesis or purpose stated earlier in the paper. Acknowledgments. Acknowledgments should appear on a separate page. Tis section also has to include the grant information (if any) of the investigators. References. Section must be double spaced and begin on a separate page. References to the literature should be cited in the text by the name of the author(s) followed by the year of publication. In cases in which there are

more than two authors, only the first is named, followed by "et al.". Examples: Smith (1980) reported that...; (Smith, 1980, 1982); (Smith and Tanaka, 1980); (Smith et al., 1980). Suffixes a, b, etc., should be used following the year to distinguish two or more papers by the same author(s) published in the same year; example (Smith, 1981a). When two or more references are included in the same bracket, they must be quoted in the chronological order; example (Smith, 1980; Bell et al., 1984). All references must be cited in the text, and all authors should be listed in references. The reference list should be in alphabetical order. References to articles in periodical publications must include: Names and initials of all authors, year of publication, complete title of paper, name of journal (abbreviated in accordance with Index Medicus), number of volume, and first and last page numbers. Example: Morita T., Suzuki Y. and Churg J. (1973). Structure and development of the glomerular crescent. Am. J. Pathol. 72, 349-368. References to books must include: Name and initials of authors, year of publication, full title, edition, editor, publisher, place of publication and page numbers. Example: Powell D. and Skrabanek P. (1981). Substance P. In: Gut Hormones. 2nd ed. Bloom S.R. and Polak J.M. (eds). Churchill Livingstone. Edimburgh. pp 396-401. References to URL (Web Page) must include: Name and initials of URL owners, full title of the URL, address, and accession date in pharantheses. Example: Stern M. Radial nerve entrapment. http://www.emedicine.com/orthoped/topic549.htm (accessed Dec 2005). Tables. Each table should be given on a separate page. Each table has a short, descriptive title. Tables are numbered in the order cited in the text. Abbreviations are defined as footnotes at the bottom of each table. Tables should not duplicate data given in the text or figures. Figures and Legends. The complete sets of original figures must be submitted. Subjects’ names must not appear on the figures. Labels should contrast well with the background. Images should be uniform in size and magnification. Illustrations should be free of all identifying information relative to the subject and institution. Line drawings should be professional in quality. Written permission for use of all previously published illustrations must be included with submission, and the source should be referenced in the legends. Written permission from any person recognizable in a photo is required. Legends must be double spaced, and figures are numbered in the order cited in the text. Submit color prints only if color is essential in understanding the material presented. Label all pertinent findings. Illustrations should be labelled with the figure number and author's name in soft pencil on the back identifying the top edge. Photographs should be glossy bromide prints of good contrast and well matched, preferably not mounted on card. Photographs should not exceed 17.8 x 22.2 cm. The Editor reserves the right to reduce or enlarge the illustrations. Colour photographs will be allowable only in special circumstances. Line diagrams should be drawn with black ink on tracing paper or white card or supplied as glossy prints. Illustrations should be submitted protected by resistant cardboard. Apply figure numbers to the lower left-hand corner of each photograph; dry transfer lettering (such as letraset) may be used. Digital images are welcome. They must be submitted on CDROM (CD-r or CD-RW) only. We cannot use other types of disk. Images should be TIFF file format, preferentially, although other formats could be useful. Black and white figures must be at gray scale. Color figures should be preferentially in CMYK, but RGB is also allowed. Line art files must have a 500dpi resolution, while other images must have a 300dpi resolution. Supplying digital images is not a substitute for the press set of figures. Technical Notes While the journal encourages the submission of full-length original articles, it will consider the publication of technical notes describing the characteristics of new instruments of methodological improvements. In addition to a title page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information), a description of the technique, and a discussion highlighting the educational value of the technique. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’ Short Communications While the journal encourages the submission of full-length original articles, it will consider the publication of short communications ensuring rapid publication of new/preliminary results of unusually educational and medically important. Whole manuscript should be maximum of 3 pages, containing maximum of 1 figure, and 1 table. In addition to a title page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information); materails and methods, results and a discussion highlighting the educational value of the case. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’

Case Reports While the journal encourages the submission of full-length original articles, it will consider the publication of concise case reports. These should be unusually educational and medically important. In addition to a title page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information); the case report (written in the past tense), and a discussion highlighting the educational value of the case. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’ Letters to the Editor Letters to the Editor may be used to describe in an extremely brief manner either an observation of interest to our readers, an opinion relative to the Cell and Tissue Biology Research, or constructive observations or criticisms of published material. Letters should be no more than two pages and should be submitted with a brief title. A maximum of four references may be included. Letters are published at the discretion of the journal and are subject to editing.

Cell and Tissue Biology Research Editorial Board Co-Editors-in-Chief: Attila DAĞDEVĠREN ġahin A. SIRMALI Managing Editor: A. Çevik TUFAN Language Editor: Selma YÖRÜKAN Statistical Advisor: Ergun KARAAĞAOĞLU Section Editors: Biomaterial Sciences & Biotechnology : Petek KORKUSUZ Cardiovascular and Endothelial Research: Candan ÖZOĞUL, Sevda F. MÜFTÜOĞLU Cell structure and function - biophysics: Selma YILMAZER, Nuhan PURALI Embryology and teratotoxicology: Deniz ERDOĞAN, Nur ÇAKAR Image Processing and Stereology: E. Oğuzhan OĞUZ Immune System and Hematology: Esin AġAN, Esra ATABENLĠ Metabolism: Digestive, Urinary and Respiratory Systems: Belgin CAN, Ufuk METE Microscopy- techniques: Zeynep KAHVECĠ, Tangül ġAN Musculoskeletal and Integumentary Systems: Serap ARBAK

Neuroscience: Hakkı DALÇIK, Özhan EYĠGÖR Proteomics- Genomics: Feride ġAHĠN, N.Lale ġATIROĞLU-TUFAN Reasearch and Academic Ethics: ġahin SIRMALI, Pergin ATĠLLA Reproductive System & Assisted Reproduction: Ramazan DEMĠR, Firdevs GÜRER Stem Cell Research- Culture: Alp CAN, Ayhan BĠLĠR Scientific Advisory Board (THEA members) Alpaslan GÖKÇĠMEN Aysel KÜKNER Aydın KETANĠ Aytekin ÖZER Belma ALABAY Bizden SABUNCUOĞLU Cengiz BAYÇU Emin Türkay KORGUN Erdoğan GÜRSOY Erinç ARAL Feral ÖZTÜRK Feriha ERCAN Figen KAYMAZ Gülten KARABAY H.Seda VATANSEVER Hikmet ALTUNAY Ġlkin ÇAVUSOĞLU Kazım TUĞYAN Leyla CANPOLAT M.Kemal ÖZBĠLGĠN Melda YARDIMOĞLU YILMAZ Meral BAKA Meral ÖNCÜ Mine YAKIġIK Mukaddes EġREFOĞLU Murat YAĞMURCA Nevin KURTDEDE Nigar VARDI Özgül TAP Sait POLAT Selçuk DUMAN Serap Sergül ĠNANÖZ Sevinç ĠNAN ġule ÇETĠNEL Süreyya CEYLAN Suzan DAĞLIOĞLU Varol ġAHĠNTÜRK Yavuz TEKELĠOĞLU Yusuf NERGĠZ

COPYRIGHT RELEASE FORM Turkish Histology and Embryology Association (THEA) Tezel sok 4/4 Yukarıayrancı, 06550 Ankara Turkey Journal title: Cell and Tissue Biology Research. Manuscript title: ............................................................................................................................. ...................

...................................................................................................................................................................... ...................................................................................................................................................................... Full names of all authors (in order to appear on manuscript): ...................................................................................... ...................................................................................................................................................................... Name, address etc. of corresponding author: ............................................................................................................ ...................................................................................................................................... ................................ ...................................................................................................................................................................... ID Number: ....................................................... Telephone: ...................................................................... E-mail: ............................................................. Mobile phone: .................................................................. The author(s) warrant(s) that: a) the manuscript submitted is his/her/their own original work; b) all authors participated in the work in a substantive way and are prepared to take public responsibility for the work; c) all authors have seen and approved the manuscript as submitted; d) the manuscript has not been published and is not being submitted or considered for publication elsewhere; e) the text, illustrations, and any other materials included in the manuscript do not infringe upon any existing copyright or other rights of anyone. Notwithstanding the above, the Contributor(s) or, if applicable the Contributor(s) Employer, retain(s) all proprietary rights other than copyright, such as a) patent rights; b) to use, free of charge, all parts of this article for the author(s) future works in books, lectures, classroom teaching or oral presentations; c) the right to reproduce the article for their own purposes provided the copies are not offered for sale. However, reproduction, posting, transmission or other distribution or use of the article or any material contained therein, in any medium as permitted hereunder, requires a citation to the Journal and appropriate credit to THEA as publisher, suitable in form and content as follows: Title of article, author(s), journal title and volume/issue, Copyright© year. All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by THEA for one year following the editorÕs decision. These materials will then be destroyed. I/We indemnify THEA and the Editors of the Journals, and hold them harmless from any loss, expense or damage occasioned by a claim or suit by a third party for copyright infringement, or any suit arising out of any breach of the foregoing warranties as a result of publication of my/our article. I/We also warrant that the article contains no libelous or unlawful statements and does not contain material or instructions that might cause harm or injury. This copyright form must be signed by all authors. Separate copies of the form (completed in full) may be submitted by authors located at different institutions; however, all signatures must be original.

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Turkish authors must supply their ID card number; foreign authors must supply their passport number.

KONGRE PROGRAMI & ÖZET KİTABI

*Kongremiz TÜBİTAK-BİDEB 2223-B Yurtiçi Bilimsel Etkinlik Düzenleme Desteği almıştır.

14. Ulusal Histoloji ve Embriyoloji Kongresi 10-13 Mayıs 2018, Antalya - Türkiye

P115 Kardeş Kromatid Değişiminin = KKD (Sister Chromatid Exchange = SCE) Oksidatif Strese Bağlı Doku Hasarının Belirlenmesinde Kullanımı Özgür Eroğlu1, İsmail Mert2, Serdar Yüksel3 1SBÜ. Van Eğitim ve Araştırma Hastanesi, Androloji Ana Bilim Dalı, Van 2Wayne State University, Department of Obstetrics and Gynecology, Detroit, United States 3T.C. Milli Eğitim Bakanlığı Kiraz MTAL, İzmir Giriş: Kardeş kromatid değişimi (KKD) herhangi bir kromozomun kardeş kromatidleri arasındaki resiprokal değişimlerdir. Değişimler aynı lokuslarda meydana geldiği için kromozom morfolojisinde bir değişiklik olmaz. Değişimlerin sebebi DNA’daki kırılmalardır ve bu kırılmalar karşılıklı kardeş kromatid değişimleri ile giderilir. KKD, ya bazı maddelerin DNA’ya kovalent bağlanması ile yada DNA tamir mekanizmalarına müdahale olması ile indüklenir. Oksidatif stresin ve toplam oksidant miktarının ve yüksek oksidant seviyesine bağlı olarak artan serbest radikalleri, malondialdehit ve F2 isoprostan gibi lipit peroksidasyonu ürünleri nin DNA tamir mekanizmalarına bir şekilde etki ettiği, KKD frekansını artırdığı düşünülmektedir. Amaç: KKD belirlemek için doku kültürü ve sitolojik preparat hazırlanması ve boyama aşamalarını optimize etmek, İntrauterin gelişme geriliği ve preeklampsili hastalarda oluşan oksidatif strese bağlı DNA hasarını KKD yöntemi ile göstermek. Materyal-Metod: Bu çalışmada kan dokusundan sağlanan lenfositler bir timin analoğu olan Bromo Deoksi Üridin (BRDU) ve normal timin içeren ortamda kültüre edilerek sitolojik preparatları hazırlandı. Yayma preparatları floresan bir boya olan Bisbenzimid ve Giemsa ile boyandı ve mikroskobik analizleri gerçekleştirildi. İntra uterin gelişim geriliği (IUGG) ile preeklampsili gebe hastaların kan örnekleri hasta grubu olarak belirlendi, kontrol grubu olarak normal gebeler seçildi. Optimize edilen hücre kültürü ve boyama yöntemi: Gerekli çözeltiler: Brdu Stok: 15,37 mg. BRDU + 10 ml distile su (filtre edilir), 32258 Hoechst stok solusyonu: 0,5 mg/ml (50mikrogr/ml) bisbenzimid distile su ile hazırlanır (+4 °C’de karanlıkta 2 hafta dayanır), 32258 Hoechst çalışma solusyonu: 1 ml hoechst stok solusyonu + 99 ml PBS solüsyonu. İlk önce 10 ml kültür ortamına (PB MAX Gibco. USA) 0,1 ml BRDU + 1 ml heparinli kan eklenir. 70 saat 37 °C’de inkübe edilir. Boyama aşamaları: 1. PBS de 5 dk. (oda ısısı) karanlık ortamda bekletilir, 2.PBS Hoechst solusyonunda 20 dk. (oda ısısı) karanlık ortamda bekletilir, 3. UV ışığında 13 cm yükseklikte PBS solusyonunda 25 dk. bekletilir Distile sudan geçirilir, 4. 65 °C de 2x SSC solusyonunda 15 dk. su banyosunda bekletilir, 5. distile sudan geçirilir, 6. %5 giemsa da 20 dk. boyanır, 7.distile sudan geçirilir. Preparat kurutulur 10x100 büyütmede ışık mikroskobunda incelenerek preparat fotoğrafları çekilir. Bulgular-Sonuç: IUGG gösteren hastalarda KKD frekansının anlamlı biçimde arttığı (7,5 e karşı 8,81, p = 0,02, n = 57) tespit edildi. Yukarıda belirtilen optimize edilmiş kültür ve boyama protokolünün başarılı ve pratik olduğu belirlendi (Şekil 1. ve Sekil 2.). Ayrıca kemik iliği gibi üretilebilen dokularda da DNA hasarının belirlenebilmesinde KKD yönteminin kullanılmasının faydalı olabileceği düşünmekteyiz. Anahtar Kelimeler: Sitoloji, Oksidatif Stres, Kardeş Kromatid Değişimi

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Şekil 1.

Kontrol grubu hastalarından birinde kromatit kırık sayısı 5 olan bir metafaz alanı Şekil 2.

IUGG hastalarından birinde kromatit kırık sayısı 21 olan bir metafaz alanı

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