Cellular Mechanisms in the Immune Response to Malaria in ...

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of immune protection of mice against blood-stage infection. (for references and ...... Langhorne, J., S. Gillard, B. Simon, S. Slade, and K. Eichmann. 1989.

INFECTION AND IMMUNITY, Oct. 1995, p. 3987–3993 0019-9567/95/$04.0010 Copyright q 1995, American Society for Microbiology

Vol. 63, No. 10

Cellular Mechanisms in the Immune Response to Malaria in Plasmodium vinckei-Infected Mice HEDVIG PERLMANN,1* SANJAI KUMAR,2 JOSEPH M. VINETZ,2† MARIKA KULLBERG,1 LOUIS H. MILLER,2 AND PETER PERLMANN1 Department of Immunology, Stockholm University, 10691 Stockholm, Sweden,1 and Laboratory of Malaria Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 208922 Received 12 May 1995/Returned for modification 14 June 1995/Accepted 1 August 1995

Infection of mice with the malaria parasite Plasmodium vinckei vinckei is 100% lethal. However, after two infections followed by drug cure, BALB/c mice develop a solid immunity which is antibody independent but mediated by CD41 T cells. To elucidate the mechanisms of this immunity, spleen cells from immune mice were challenged in vitro with lysates of P. vinckei-infected or uninfected erythrocytes. The parasite antigen induced proliferation of T cells from immune mice but not from nonimmune mice. When gamma interferon production by cells from immune mice was assayed at the single-cell level, 1 to 3 cells per 1,000 cells were found to release this cytokine when exposed to antigen. In contrast, the numbers of interleukin 4 (IL-4)-producing cells from both immune and control mice were

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