centrifuge AB FH SBM CD FH FH and SBM separation press ... - MDPI

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CD34+ HSC/HSPC. FSC. SSC. CD56. CD3 granulocytes lymphocytes. CD3+CD56+ NKT cells. CD3-CD56+ NK cells. CD3. CD4. CD45RA. CD25. CD3+CD4+ T ...
A

C

FH and SBM B

FH

separation

press through cell strainer

FH

centrifuge D supernatant cells

SBM

centrifuge

Exclusion of debris and dead cells

PBMC

FSC

CD34+ HSC/HSPC

SSC

SSC

SSC

CD14+ monocytes

CD14

CD34

CD16+ granulocytes

granulocytes

SSC

CD3

CD16

FSC

IgD-CD27+ B cells

CD3-CD56+ NK cells

IgD+CD27+ B cells

CD27

CD3

CD3+CD56+ NKT cells

IgD+CD19+ B cells

IgD

CD56

CD25+CD45RA- T helper cells CD3+CD4+ T helper cells

CD4

CD25

CD25+CD45RA+ T helper cells

CD3

CD45RA

CD25+CD127- Treg cells

CD45RA

CD25

CD3+CD8+ cytotoxicT cells

CD8

CD3

CCR7+CD3+CD4+ T helper cells

CCR7

CD127

CD127

CD127+CD3+CD4+ T helper cells

CD45RA

CD45RA+ cytotoxicT cells

CD25

SSC

lymphocytes

CD19

CD19+CD3- B cells

CD45RA