Appl Microbiol Biotechnol (2012) 95:1615–1623 DOI 10.1007/s00253-011-3831-0
ENVIRONMENTAL BIOTECHNOLOGY
Changes in functional diversity of soil microbial community with addition of antibiotics sulfamethoxazole and chlortetracycline Feng Liu & Jinshui Wu & Guang-Guo Ying & Zhuanxi Luo & Hong Feng
Received: 16 May 2011 / Revised: 2 December 2011 / Accepted: 9 December 2011 / Published online: 29 December 2011 # Springer-Verlag 2011
Abstract Potential effects of antibiotics on agricultural soil microflora have recently become increasing concerns with antibiotic-contaminated biosolid now being used in agricultural land. However, changes of soil microbial community function caused by the antibiotic-associated disturbance are less addressed. This paper investigated the changes in microbial functional diversity by spiking sulfamethoxazole (SMX) and chlortetracycline (CTC) in a loam paddy soil and then incubating for 21 days. The dose–effect and timedependent changes of antibiotic-associated disturbance on soil microbial community were analyzed with the soils sampled at 7 and 21 days using Biolog EcoPlate. At day 7 following treatment, SMX decreased functional diversity of soil microbial community, and the treatment of 100 mg SMX kg−1 dry soil had a significant inhibition of average F. Liu : J. Wu Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China F. Liu e-mail:
[email protected] F. Liu : G.-G. Ying (*) State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China e-mail:
[email protected] Z. Luo Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China H. Feng College of Resource and Environmental Science, South China Agricultural University, Guangzhou 510640, China
well color development (AWCD) and Shannon index as compared to the control (p
