Characterization of enterovirus 71 infection and

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SW12078. ——. ——. ——. SW12079 positive —— positive. SW12080. ——. —— positive. SW12081. ——. —— positive. SW12082 positive —— positive.

Characterization of enterovirus 71 infection and associated outbreak of Hand, Foot, and Mouth Disease in Shawo of China in 2012 Michelle Y. Liu1,2,3#, Jin Liu2#, Weijian Lai1,4,5, Jun Luo2, Yingle Liu2, Gia-Phong Vu3, Zhu Yang4,5, Phong Trang3, Hongjian Li1*, Jianguo Wu2*

1

College of Life Sciences, Jinan University, Guangzhou, Guangdong 510632, China

2

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei

430072, China 3

School of Public Health, University of California, Berkeley, CA 94720

4

Taizhou Institute of Virology, Taizhou, Jiangsu 225300, China

5

Jiangsu Affynigen Biotechnologies, Inc., Taizhou, Jiangsu 225300, China

#

These authors contribute equally to this study.

*Correspondence should be addressed to: Dr. Jianguo Wu State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China, Tel: +86-27-68754001, E-mail: [email protected] Dr. Hongjian Li College of Life Sciences, Jinan University, Guangzhou, Guangdong 510632, China. Tel: +86-2085220501, Email: [email protected]

1

SUPPLEMENTAL INFORMATION Table S1. Quantitative RT-PCR detection of the EV-A71, CV-A16, and human enterovirus universal (EVU) sequences in the HFMD samples.

Number

EV-A71

CV-A16

EVU

SW12001 SW12002 SW12003 SW12004 SW12005 SW12006 SW12007 SW12008 SW12009 SW12010 SW12011 SW12012 SW12013 SW12014 SW12015 SW12016 SW12017 SW12018 SW12019 SW12020 SW12021 SW12022 SW12023 SW12024 SW12025 SW12026 SW12027 SW12028 SW12029 SW12030 SW12031 SW12032 SW12033

——a —— —— positive positive positive positive —— —— —— —— —— positive positive positive —— positive —— —— positive —— —— positive —— —— —— —— positive positive —— positive positive ——

—— —— —— —— positive positive positive —— —— —— —— positive positive —— —— positive positive —— —— —— —— positive —— —— —— —— —— —— —— positive —— —— ——

—— —— —— positive positive positive positive —— —— —— —— positive positive positive positive positive positive —— —— positive —— positive positive —— —— —— positive positive positive positive positive positive ——

SW12034 SW12035 SW12036 SW12037 SW12038 SW12039 SW12040 SW12041 SW12042 SW12043 SW12044 SW12045 SW12046 SW12047 SW12048 SW12049 SW12050 SW12051 SW12052 SW12053 SW12054 SW12055 SW12056 SW12057 SW12058 SW12059 SW12060 SW12061 SW12062 SW12063 SW12064 SW12065 SW12066 SW12067 SW12068 SW12069 SW12070 SW12071 SW12072 SW12073 SW12074 SW12075 SW12076

—— —— —— —— —— —— —— —— —— —— —— positive —— positive positive positive positive —— positive positive positive —— —— —— positive positive —— —— positive positive —— positive —— positive —— positive —— positive —— positive positive —— positive

—— —— —— —— positive positive —— —— —— —— —— —— —— —— —— —— —— —— —— —— —— —— positive —— —— —— positive —— —— —— —— —— —— —— —— —— —— —— —— positive —— —— ——

—— —— —— —— positive positive positive —— —— —— —— positive —— positive positive positive positive —— positive positive positive positive positive positive positive positive positive —— positive positive —— positive positive positive positive positive —— positive positive positive positive positive positive

SW12077 SW12078 SW12079 SW12080 SW12081 SW12082 SW12083 SW12084 SW12085 SW12086 SW12087 SW12088 SW12089 SW12090 SW12091 SW12092 SW12093 SW12094 SW12095 SW12096 SW12097 SW12098 SW12099 SW12100 SW12101 SW12102 SW12103 SW12104 SW12105 a Negative;      

positive —— positive —— —— positive positive —— positive —— positive positive —— —— positive positive —— positive positive —— —— —— —— —— positive positive positive positive ——

—— —— —— —— —— —— —— —— positive —— —— —— —— —— positive —— —— —— —— —— —— —— —— —— —— —— —— —— ——

positive —— positive positive positive positive positive —— positive —— positive positive —— —— positive positive —— positive positive —— —— —— —— —— positive positive positive positive ——

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