Characterization of Humoral Immune Responses against Capsid ...

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Nov 1, 2016 - did not react with any of the three HIV-1 p24 peptides carrying IDEs, but did ...... Barin F, McLane MF, Allan JS, Lee TH, Groopman JE, Essex M.

RESEARCH ARTICLE

Characterization of Humoral Immune Responses against Capsid Protein p24 and Transmembrane Glycoprotein gp41 of Human Immunodeficiency Virus Type 1 in China a11111

Xiufen Li1, Yue Wu1, Xuqi Ren2, Shuyun Deng3, Guifang Hu1, Shouyi Yu1*, Shixing Tang1* 1 Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, China, 2 Department of Sexually Transmitted Diseases, Guangdong Provincial Skin Diseases and STD Control Center, Guangzhou, Guangdong, China, 3 State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China

OPEN ACCESS Citation: Li X, Wu Y, Ren X, Deng S, Hu G, Yu S, et al. (2016) Characterization of Humoral Immune Responses against Capsid Protein p24 and Transmembrane Glycoprotein gp41 of Human Immunodeficiency Virus Type 1 in China. PLoS ONE 11(11): e0165874. doi:10.1371/journal. pone.0165874 Editor: Yuxian He, China Academy of Chinese Medical Sciences, CHINA Received: June 27, 2016 Accepted: October 19, 2016 Published: November 1, 2016 Copyright: © 2016 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files.

* [email protected] (ST); [email protected] (SY)

Abstract The objective of this study was to extend our previous research and to further characterize the humoral immune responses against HIV-1 p24, gp41 and the specific peptides carrying the immunodominant epitopes (IDEs) that react with human serum samples from HIV-1infected individuals in China. We found that the majority (90.45%, 180/199) of the samples did not react with any of the three HIV-1 p24 peptides carrying IDEs, but did react with the recombinant full-length p24, suggesting that these samples tested in China were primarily directed against the conformational epitopes of HIV-1 p24. In contrast, 84.54% (164/194) of the samples reacted with at least one HIV-1 linear gp41 peptide, in particular the gp41p1 peptide (amino acids 560–616). Both recently and long-term HIV-1-infected individuals displayed similar humoral immune responses against the recombinant gp41. However, samples from long-term HIV-1-infected subjects but not from recently infected subjects, showed a very strong reaction against the gp41-p1 peptide. The different response patterns observed for the two groups against the gp41 and the peptide gp41-p1 were statistically significant (P

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