obesity, insulin resistance and diabetes in mice fed high-fat and cafeteria diets. ..... Sterling Heights, MI, USA) attached to a JVC camera. Model KY-F55B ...... Dupre J, Greenidge N, McDonald TJ, Ross SA, Rubinstein D. (1976) Inhibition of ...
Diabetologia (2007) 50:1752–1762 DOI 10.1007/s00125-007-0710-4
ARTICLE
Chemical gastric inhibitory polypeptide receptor antagonism protects against obesity, insulin resistance, glucose intolerance and associated disturbances in mice fed high-fat and cafeteria diets V. A. Gault & P. L. McClean & R. S. Cassidy & N. Irwin & P. R. Flatt
Received: 13 February 2007 / Accepted: 19 April 2007 / Published online: 9 June 2007 # Springer-Verlag 2007
Abstract Aims/hypothesis Gastric inhibitory polypeptide (GIP) receptor antagonism with (Pro3)GIP improves glucose tolerance and ameliorates insulin resistance and abnormalities of islet structure/function in ob/ob mice. This study examined the ability of (Pro3)GIP to counter the development of obesity, insulin resistance and diabetes in mice fed high-fat and cafeteria diets. Materials and methods Young Swiss TO mice on standard chow or high-fat, cafeteria or high-carbohydrate diets received daily injections of either saline or (Pro3)GIP (25 nmol kg-1day-1) over 16 weeks. Food intake, body weight, and circulating glucose and insulin were measured frequently. At 16 weeks, glucose tolerance, insulin sensitivity, HbA1c, circulating hormones and plasma lipids were assessed. Adipose tissue, liver and muscle were excised and weighed, and their histology and triacylglycerol content were further examined. Results (Pro3)GIP significantly reduced body weight, enhanced locomotor activity, and improved HbA1c, glucose tolerance, beta cell responsiveness and insulin sensitivity in mice fed high-fat and cafeteria diets (p
