Chitosan-DNA Nanoparticles: The Effect of Cell Type and Hydrolysis ...

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Chitosan-DNA Nanoparticles. Kadir Turan. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Marmara, Uskudar, Istanbul, Turkey.
Pharmaceutical Development and Technology, 11:503–512, 2006 Copyright © Informa Healthcare ISSN: 1083-7450 print / 1097-9867 online DOI: 10.1080/10837450600940873

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Chitosan-DNA Nanoparticles: The Effect of Cell Type and Hydrolysis of Chitosan on In Vitro DNA Transfection Kadir Turan Chitosan-DNA Nanoparticles

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Marmara, Uskudar, Istanbul, Turkey

Kyosuke Nagata Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan

Commercial chitosan (Ch) with low (LMWCh) and medium molecular weight (MMWCh) were hydrolyzed in diluted hydrochloric acid by heating at different temperatures. The viscosity average molecular weight of Chs was gradually decreased from 450 to 14 kDa as a function of temperature. Ch fractions were used for formation of Ch-DNA nanoparticles and tested for the ability to introduce DNA into HEK293, Swiss3T3, HeLa, and MDCK cells in vitro. The average diameter of nanoparticles was 200–220 nm. The surface charge of nanoparticles varied depending on the Ch/DNA ratio. The cell lines different response to DNA transfection with Ch fractions depended on molecular weight. HEK293 cells were efficiently transfected by nanoparticles prepared with Chs having a wide range of molecular weight (∼14–195 kDa). Swiss3T3 cells were efficiently transfected by Ch polymers with about