P5. Proteomic analysis of canine vascular endothelial cells (CnAOEC) and smooth muscle cells (CnAOSMC) stimulated with Dirofilaria immitis extracts by LC-MS/MS. Morchón R.1, González-Miguel J.2, Sotillo J.3, Carretón E.4, Diosdado A.1, Zueva T.1, Siles-Lucas M.2, Montoya J. A.4, Simón F.1 1
Laboratory of Parasitology, Animal and human dirofilariosis group, Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno s/n, 37007 Salamanca, Spain. 2 Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), 37008 Salamanca, Spain. 3 Centre for Biodiscovery and Molecular Development of Therapeutics, Building E4, James Cook University, McGregor Rd., Smithfield, Townsville, QLD, 4878, Australia. 4 Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35001 Las Palmas, Spain. Correspondence: Rodrigo Morchón (
[email protected])
The cardiopulmonary dirofilariosis caused by Dirofilaria immitis is a chronic disease characterized by the development of acute inflammatory reactions and thrombi formation in the pulmonary arteries of the hosts. When spontaneous or treatment induced worm’s death exacerbates inflammatory reactions at the levels of pulmonary arteries, producing a massive release of antigenic products. Nevertheless, adult worms survive for years in immunocompetent hosts. To better understand the influence of worms on the blood vessel endothelium, in the context of the D. immitis/host relationships, cultures of canine vascular endothelial cells (CnAOEC) and smooth muscle cells (CnAOSMC) were treated with somatic antigens of D. immitis adult worms during the first 24 hours. In both cases unstimulated cells were used as controls. Treated and untreated cells were lysed and analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). As regards CnAOEC, a total of 2573 proteins were identified of which 1308 were in control cells and 1265 in stimulated cells. Of these, 337 (21%) have only been identified in control cells, 294 (18.4%) in stimulated cells and 971 (60.6%) in both. For CnAOSMC, 937 proteins were identified in control cells and 979 in stimulated cells. Of all of them, 224 (18.6%) only appeared in control cells, 266 (22.1%) in stimulated cells and 713 (59.3%) in both. Many of these proteins in previous works have been involved in the parasite / host relationship such as heat shock proteins, stress response, energy metabolism and redox processes. The differences between some types of cells and others and their similarities are discussed.
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ISBN: 978-84-09-02065-2
