Cocoon-Derived Silk Membrane, Collagen Membrane ...

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We thank to Sang-Woon Lee (Gangneung. Asan Hospital) for revising ... Rhyu, S. B. Han, and C. P. Chung, J. Biotechnol., 120, 327. (2005). (12) G. H. Altman, F.

Macromolecular Research, Vol. 22, No. 9, pp 1018-1023 (2014) DOI 10.1007/s13233-014-2138-2

www.springer.com/13233 pISSN 1598-5032 eISSN 2092-7673

Comparison of the Physical Properties and In vivo Bioactivities of SilkwormCocoon-Derived Silk Membrane, Collagen Membrane, and Polytetrafluoroethylene Membrane for Guided Bone Regeneration Yong-Yun Ha1, Young-Wook Park1, HaeYong Kweon2, You-Young Jo2, and Seong-Gon Kim*,1 1

Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangwon 210-702, Korea 2 Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, Gyeonggi 441-707, Korea Received March 10, 2014; Revised June 3, 2014; Accepted June 9, 2014

Abstract: The aim of this study was to evaluate silkworm-cocoon-derived silk membrane (SM) for application in guided bone regeneration (GBR) and to compare it with commercially available GBR membranes, such as collagen membrane (CM) and polytetrafluoroethylene membrane (PM). The SM, CM, and PM membranes were examined via scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) analysis, and tensile strength evaluation. Thirty rats were used for the experiments. Critical-sized parietal bone defects (diameter: 8.0 mm) were divided into three groups according to the applied GBR membrane. The rats were sacrificed 4 or 8 weeks after surgery and evaluated by micro-compiterized tomography (µ-CT) and histology. The FTIR analysis of the SM was similar to that of the CM. The SEM images showed that the surface characteristics of the SM differed from those of the CM and PM. The SM showed a complex network structure of silk fibers. The wet SM was also more resistant to tensile stress than the wet CM and PM. The µ-CT results of the animal study showed that the bone volume of the SM group was higher than those of the CM and PM groups 4 weeks after the operation (p

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