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Anal Bioanal Chem (2014) 406:7591–7600 DOI 10.1007/s00216-014-8199-4

RESEARCH PAPER

Colorimetric detection of acetylcholine with plasmonic nanomaterials signaling Ju-Hwan Oh & Byoung Chan Kim & Jae-Seung Lee

Received: 19 June 2014 / Revised: 14 September 2014 / Accepted: 17 September 2014 / Published online: 26 September 2014 # Springer-Verlag Berlin Heidelberg 2014

Abstract We have developed a highly sensitive and selective colorimetric method for detection of acetylcholine (ACh), using a tandem enzymatic reaction for biological target recognition and silver nanoplates (AgNPLs) for optical signal generation. The ACh molecules are enzymatically hydrolyzed and oxidized into betaine and hydrogen peroxide, the latter of which chemically oxidizes the AgNPLs to generate the “turnon” signal. To optimize detection sensitivity, the chemical and biological properties of the detection mixtures containing the enzymes, ACh, and AgNPLs were thoroughly investigated with respect to component concentrations and reaction temperatures; a maximum sensitivity of 500 nM for colorimetric detection of ACh was achieved. We further directly compared the signaling profiles of (1) novel nanostructured and (2) conventional molecular chromogens, improving our understanding of the factors that should be considered when designing a detection system.

Keywords Nanoparticles/nanotechnology . Biosensors . Optical sensors . Enzymes

Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-8199-4) contains supplementary material, which is available to authorized users. J.