communications

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the applicability of a kinetic microassay to quantify catalase from two different sources. The assay was .... peroxidase–encoding genes, katE and katG, respec-.
Journal of Biomolecular Techniques 18:185–187 © 2007 ABRF

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communications Rapid Kinetic Microassay for Catalase Activity Yi Li and Herb E. Schellhorn McMaster University, Hamilton, Ontario, Canada Catalase is a commonly assayed enzyme found in many bacteria and eukaryotes. In this report, we examined the applicability of a kinetic microassay to quantify catalase from two different sources. The assay was found to be linear over a wide range (0.1–1.0 units), but was limited at high values (>1 unit) by oxygen evolution. Nonetheless, the micorassay allows simultaneous evaluation of many samples (up to 96) in a short time (