CONTROL OF MOLECULAR WEIGHT

TECHNICAL REPORT NATICK/TR-88/012

CONTROL OF MOLECULAR WEIGHT DISTRIBUTION OF THE BIOPOLYMER PULLULAN PRODUCED BY THE FUNGUS Aureobasidium pullulans

BY B. J. WILEY S. ARCIDIACONO S. SOUSA J. M. MAYER D. L. KAPLAN OCTOBER 1987 FINAL REPORT 1986-1987

APPROVED FOR PUBLIC RELEASE; DISTRIBUTION UNLIMITED

UNITED STATES ARMY NATICK RESEARCH, DEVELOPMENT AND ENGINEERING CENTER NATICK, MASSACHUSETTS 01760-5000 SCIENCE & ADVANCED TECHNOLOGY DIRECTORATE

DISCLAIMERS

The findings contained in this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents.

Citation of trade names in this report does not constitute an official endorsement or approval of the use of such items.

DESTRUCTION

NOTICE

For Classified Documents: Follow the procedures in DoD 5200.22-M, Industrial Security Manual, Section 11-19 or DoD 5200.1-R, Information Security Program Regulation, Chapter IX

For Unclassified/Limited Distribution Documents: Destroy by any method that prevents disclosure of contents or reconstruction of the document.

V VTß "J 9 '-W-V* ■>■ W^

MftVJ^WILVJAWWJF^^^

UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE form Approved OMB No 0704-0788 f*p Oafe /un lunjOf 1JM ftp Jt* >J

REPORT DOCUMENTATION PAGE la REPORT SECURITY CLASSIFICATION

lb RESTRICTIVE MARKINGS

UNCLASSIFIED i* SECURITY CLASSIFICATION AUTHORITY

3

DISTRIBUTION /AVAILABILITY Of REPORT

2b (^CLASSIFICATION/OOWNGRAOING SCHEOULE

Approved for public release; distribution unlimited.

4 PERFORMING ORGANIZATION REPORT NUMBER(S)

S. MONITORING ORGANIZAT'ON REPORT NUMBER(S)

NATICK/TR-88/012 6a NAME Of PERFORMING ORGANIZATION

?a NAME OF MONITORING ORGANIZATION

6b OfflCE SYMBOL (tf applicable)

STRNC-YEP

U. S. Army Natick RD&E Center

7b

f>c ADDRESS (dry. Statt, and ZIP Code)

Natick, MA

01760-5020

8a NAME OF ENDING/SPONSORING ORGANIZATION

9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER

8b OFflCE SYMBOL (tf applicable)

STRNC-YEP

U. S. Army Natick RD&E Center 8t ADORES* (Gty. State, and ZIP Code)

Natick, MA "

*ITL£

U)

ADDRESS [City, State, and Z'PCode)

10 SOURCE OF FJND'NG NUMBERS PROGRAM PRO;ECT ELEMENT NO NO

01760-5020

TASK NO

07

1L161101A91A

61101A

WORK UNIT ACCESS ON NO

144

(include Security ClaiSificationj

CONTROL OF MOLECULAR WEIGHT DISTRIBUTION OF THE BIOPOLYMER PULLULAN PRODUCED BY THE FUNGUS Aureobasidium pullulans

'2 PERSONAL AUT«0R(S)

B. J. Wiley, S. Arcidiacono, S. Sousa, J. M. Mayer, and D. L. Kaplan >3a

T

YPE

OF REPQR1

i3b TIME COVERED

Final

FROM86 Mar

14 DATE OF REPORT {Year, Month. Day)

15 PAGE COUN1

30

1987 October

TO

'6 SuPPLtMENTARV NOTATION

COSAT: CODES

V E.D —i,

GROUP

18

SUB-GROUP

T

SU8JEC

2tUNGUS9

T

A&$TRAC

TER^S

[Continue on reverie it necesssry and identify by block number)

BI0P01YMFR -^NUTRIENTS;, -x PULLULAN / FERMENTATION^

CHEMICAL PROPERTIES PHYSICAL PROPERTIES

CARBON NITROGEN

^POLYSACCHARIOES» MOLECULAR WEIGHT

r

Continue on reve ie. it necenary and oentity by block number)

This report summarizes one section or this study, the evaluation of environmental conditions and their influence on the biopolymer pullulan molecular weight distribution and yield, "ilms, fibers, and chemical derivatives made from these different molecular weight products, and the characterization of these materials will provide the bases for another report. In order to determine optimum growth conditions for the control of molecular weight distribution of extracellular pullulan, preliminary studies utilized cell suspensions of nine strains of Aureobasidium puljuil_ans. One Strain, NRRL-Y 6220 A. pullulans, was selected for further study Carbon '"and* nitrogen so'urces, along with phosphate concenTratTon were evaluated for their effec bn pullulan yield and molecular weight distribution. Batch systems, scale-up batch, and continuous fermentations of one liter and 10 liters were also evaluated. Processing variables including solvents, extraction time, etc., were also studied. Pullulan biopolymer products with weight average molecular weights from 100 thousand to 4 million, with a disporsity of around two, were produced. The evaluation of chemical/physical properties of defined molecule^ (weight fractions of pullulan is now under investigation.^ ^ti^Oi y\Z*j ?0

DISTRIB'J^ON.

AVAILABILITY Of ABSTRACT

QjNCLASSlF'EO'UNLlMITED

£} SAME AS PPT

2a NAME 0 = 'RESPONSIBLE iNCCVOUA.

[Bonnie J. Wi ley DD FORM 1473, 84

MAR

^rffrSffirara^^^^

21 Q OTiC USERS

ABSTRACT SEC'JP'TV CuASSiKATlON »tCs,

UNCLASSIFIED 22b

TELEPHONE

(include AreaCode)

83 APR eo'tton nay be uie>y>%>sy^^^^^

v«v.v.y