J. Phycol. 40, 619 (2004) r 2004 Phycological Society of America DOI: 10.1111/j.1529-8817.2004.40301.x
CORRIGENDUM Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades (Vol. 37:1138–1145) J. A. Berges, D. J. Franklin, and P. J. Harrison Table 2 is reprinted below. The g L
1
quantities of EDTA indicated in Metals Stock I and II were switched.
TABLE 2. ESAW medium as modified from Harrison et al. (1980) and ESAW medium over the past two decades. Footnotes highlight the changes from the original recipe. gL
1
stock solution
Final concentration in media
a
Salt solution I—anhydrous salts NaCl Na2SO4 KCl NaHCO3 KBr H3BO3b NaF Salt solution II—hydrated saltsa MgCl2 6H2O CaCl2 2H2O SrCl2 6H2O Major nutrient I—nitrate [1 mL L 1] NaNO3 Major nutrient II—phosphate [1 mL L 1]c NaH2PO4 H2O Major nutrient III—silicate [2 mL L 1]d Na2SiO3 9H2O Metals stock I—iron [1 mL L 1]e FeCl3 6H2O Na2EDTA 2H2O Metals stock II—trace metals [1 mL L 1]f ZnSO4 7H2O CoSO4 7H2O MnSO4 4H2O Na2MoO4 2H2Og Na2SeO3g NiCl2 6H2Og Na2EDTA 2H2O Vitamin stock [1 mL L 1]h Thiamine-HCl Biotin B12
21.19 3.55 0.599 0.174 0.0863 0.0230 0.0028
363 mM 25.0 mM 8.04 mM 2.07 mM 725 mM 372 mM 65.7 mM
254 nM 5.69 nM 2.42 mM 6.1 nM 1 nM 6.3 nM 8.29 mM 297 nM 4.09 nM 1.47 nM
Anhydrous and hydrated salts must be dissolved separately; masses assume specific gravity 5 1.021 at 201 C. Borate now added only in salt solution, not in trace metals. c New phosphate source removes the organic glycerophosphate. d Silicate stock solution is not acidified and is made at half previous strength to facilitate dissolution. e Iron now added solely as chloride (removes ammonium) in a separate stock with equimolar EDTA. f Trace metal stock now made 10 original concentration. EDTA must be dissolved first. For convenience, each metal can be added at 10 mL L 1 from individual 100 concentrated stocks. g New addition to recipe. h Filter sterilize; store frozen. b
