cover cell lines - p53 WEB SITE - Free

HANDBOOK OF p53 MUTATION IN CELL LINES Version 1.0 07/2007

24 h S

Control

G2 G1

3h

8h 24 h

Adriamycin 3h

8h Adriamycin (1h. pulse)

3h

24 h

8h γ Irradiation

24 h

DNA content (propidium iodide) 3h

8h

Thierry Soussi http://p53/free.fr [email protected]

Table of Contents Just click to access a specific section of this document Foreword

1

All cell lines with wt p53

4

All p53 null cell lines

11

All cell lines with splice mutations

12

All cell lines with controversial p53 mutations

13

All cell lines with p53 mutations (controversial and confirmed)

18

Cell lines for common cancer (wt, null, splice and mutant) Bladder carcinoma

57

Nervous System (Astrocytoma + Neuroblastoma + Glioblastoma)

60

Breast carcinoma

64

Colorectal carcinoma

68

Gastric carcinoma

72

Gynecological tumours (ovarian, endometrial and cervical)

75

Hepatocellulat carcinoma

79

HNSCC

81

Leukemia/Lymphoma

87

Melanoma

94

NSCLC

97

Pancreatic carcinoma

102

Prostate carcinoma

105

Sarcoma

107

SCLC

113

References

114

Welcome to version 1.0 of the handbook of p53 mutations in human cancer cell lines A survey of the literature (1989-2007) reveals discrepancies in the p53 status for 20 to 25% of human cancer cell lines (Berglind et al. manuscript submitted) This is a very serious problem, as knowledge of p53 status is essential for the design and interpretation of many experiments. There are many causes for these discrepancies: -

Cell line cross-contamination, which is a very serious problem. A recent estimation suggests that 20% of cell lines are cross-contaminated.

-

Incorrect sequencing strategy, as many studies have only focused on exons 5 to 8 (or 4 to 9).

-

Mislabelling of cell line names.

We do not believe that some of these discrepancies are due to p53 gene neomutations occurring during cell culture. This chaotic situation is further complicated by semantic problems associated with the nomenclature of p53 status in cell lines. For example, the “p53-null” status is used in different ways in the literature. The two most common meanings are a cell line with a documented p53 gene deletion (both alleles) or a cell line with a p53 mutation. We have also observed more “unusual” situations in which this status is only based on p53 expression (RNA or protein). Unfortunately, this type of information diffuses rapidly in the literature without any verification of the original publication. This is the case for the anaplastic thyroid carcinoma cell line, FRO, which is frequently referred to as p53-null and is used as a recipient for transfection of wt and mutant p53. The original reference for the analysis of the p53 gene status in this cell line is always correctly quoted, but a closer look at this original paper demonstrates a marked decrease of p53 RNA in the cell but no mutation was detected by sequencing exons 5 to 8. Either a mutation is situated outside this region leading to a decrease of RNA expression (frameshift mutation associated with Nonsense-Mediated mRNA Decay) or the altered p53 expression is due to another mechanism. Whatever the reason for the absence of p53 expression, the FRO cell line should obviously not be called a “p53-null”. Many examples of this type of situation can be found in the literature. Another reason why “p53-null” should be used cautiously to describe cell lines that express mutant p53 is the observation that p53 mutations are fairly heterogeneous in terms of loss of function and several cell lines display a normal or partial p53 response. Finally, there is now ample evidence that some mutant p53 behave as dominant oncogenes with a gain of function activity. We therefore believe that the “p53 null” status should be used only for cell lines that are totally devoid of p53 gene. Any other situation should be referred to as “mutant p53”.

Cell line p53 Handbook

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v 1.0

This version of the p53 handbook includes the p53 status of 1,300 cell lines. Four tables are presented for each type of cancer: Table I: cell lines with a wt p53 gene. Although no genetic alteration is found in the p53 gene of these cell lines, it is possible that other mechanisms may lead to p53 inactivation (mdm-2 amplification for sarcoma, nuclear exclusion for neuroblastoma, HPV infection for cervical carcinoma) Table II: cell lines with p53 gene deletion or rearrangement (true “p53 null” cell lines). Table III: cell lines with splice mutations. Table IV: cell lines with missense or small frameshift mutations. Some of these frameshift mutations are associated with loss of p53 expression by NonsenseMediated mRNA Decay (K562 cells). This is version 1.0 of this handbook. This chaotic situation will be progressively resolved with the accumulation of novel and accurate data. This handbook and the p53 web site will be regularly updated. You are strongly encouraged to share your data with us for the benefit of the scientific community. Please let us know if you find any errors: [email protected]


2

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Description of the table Files have been sorted by cancer and by cel lines name

Pos WT Mut

Codon position (1 to 393) Normal base sequence of the codon in which the mutation occurred Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown. AA Wild type amino acid MutAA Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: Inframe insertion or deletion Comp SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 allele; DMD (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor. Name Name of the tumor/patient/cell line as given by the authors. If the publication does not include any sample name, we have arbitrarily assigned a name, usually the first letters of the last author's name, followed by the numbers in the series. The same name or number can occur several times in a single study as in some samples more than one mutation has been reported (see complexity to find samples with multiple mutations). For cell lines, we used the name given in the publication. Unfortunately, there is some confusion as similar cell lines can appear with multiple names (acronym or ATCC references, change/errors in the acronym). Care has been taken to ensure some homogeneity NB Number of tumors with this particular mutant the database Cancer Cancer name Ref Reference identification number WAF1 Residual transcriptional activity of mutant p53 on the WAF1 promoter (% compared to wild type p53). Data for WAF1 are taken from the work of Kato et al.,( Kato S, Han SY, Liu W, Otsuka K, Shibata H, Kanamaru R, Ishioka C (2003) Understanding the functionstructure and function-mutation relationships of p53 tumor suppressor protein by high-resolution missense mutation analysis. Proc Natl Acad Sci U S A 100: 84248429). Transactivation was tested using a yeast assay. The residual transcriptional activity of mutant p53 is always compared to wild type p53 for the same promoter (%). Wt: mutation that does not change the amino acid: Be aware that some of these mutation can change splicing or RNA stability Fr: Frameshift mutations. No activity data is available but it is usually assumed that no p53 is produced Tr: Terminating mutation: No activity data is available but it is usually assumed that no p53 is produced ND: No data available for this mutant. Cell line p53 Handbook

3

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Brain Tumors (wt p53 status) Cell line CHP-134* KG-1-C GOTO IMR-32* LAN-5* NB-1 NH-12 NH-6 SK-N-AS* SK-N-SH* U-87-MG GM2300 GM1578 GM2455 GM1600 GM1592 GM139 GM2401 SK-MG-11 SK-MG-15 V-MG-33

ATCC

CCL-127

CRL-2137 HTB-11 HTB-14

Origin

Reference

Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

1006 1006

1006 1006 1006

2249 1950 1950 1950 1950 1950 1950 1950 698 698 698

* Cytoplasmic p53

Breast Tumors (wt p53 status) Cell line MCF-7 MCF10-2A DU4475 MDA-MB175VII MPE600 SK-BR-7 SUM102PT UACC-812 ZR75-1 ZR75-30 HBL-100* MRK-nu-1 YMB-1 YMB-1-E

ATCC HTB-22

Reference 2091

HTB-123

2091

HTB-25

2091 2091 2091 2091 2091 2091 2091 2029 1006 1006 1006

CRL-1897 CRL-1500 CRL-1504

Positive for SV40


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Cervical carcinoma (wt p53 status) Cell line HeLa* ME-180* SKG-IIIa* SW756* CA-SKI* SIHA* KB* MS-715* C4II*

ATCC CCL-2 HTB-33 CRL-10302 CRL-1550 HTB-35 CCL-17 HTB-34 CRL-1595

SKG-II*

Reference 1006 1006 1006 2249 2249 2249 2249 68 68 leave blank for now

* HPV positive

Colorectal Tumors (wt p53 status) Cell line C32 C99 COLO678 Gp2D HCT116 LOVO LS180 LS174T LS513 NCI-747 RKO SKCO-1 Col15 TC7 EB

ATCC

CCL-247 CCL-229 CL-187 CL-188 CRL-2134 CRL-2577 HTB-39

Reference 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2251 2251 2251

Gastric Tumors (wt p53 status) Cell line SNU-520 SNU-719 NUGC-4 STKM-2 MKN-45 MKN-74


ATCC

-

5

Reference 747 747 462 1006 462 462

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Head and Neck SCC (wt p53 status) Cell line MO24 PCI-30 UM-SCC-17A/B UM-SCC-25 UM-SCC-47 UM-SCC-72 UM-SCC-74A UM-SCC-74B UM-SCC-81A UPCI-SCC-3 UPCI-SCC-30 UPCI-SCC-40 UPCI-SCC-56 UPCI-SCC-81 UPCI-SCC-104 UPCI-SCC-131 UPCI-SCC-142 UPCI-SCC-154 HOC927 BICR-10 BICR-18 MSK-922 MSK-121 MDA-1986 MDA-886 584 185 1483 183A NHOK HOK-16B HOK-18A HAp-2

ATCC

Reference 2252 2252 2252 2252 2252 2252 2252 2252 2252 1544 1544 1544 1544 1544 1544 1544 1544 1544 105 208 208 600 600 600 600 600 600 600 600 626 626 626 626

Hepatocellular carcinoma (wt p53 status) Cell line HuH-6 clone 5 HuH-1* HCC-M* SK-Hep1 HepG2 WRL 68 FOCUS*,**

ATCC

HTB-52 HB-8065

Reference 1006 1042 1042 1042 1042 230 232

*HBV positive Cell line p53 Handbook

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Leukemia / Lymphoma (wt p53 status) Cell line J-111 IM-9 ML-1 KMS-12-BM KMS-12-PE RPMI-1788 Ly3

ATCC

Origin AML CML-B CML Myeloma Myeloma Myeloma Diffuse large cell lymphoma Diffuse large cell lymphoma Myeloma Myeloma Myeloid Leukemia ALL ALL ALL ALL ALL ALL ALL Burkitt lymphoma Lymphoblastoid Lymphoblastoid Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma ALL ALL ALL ALL ALL ALL ALL ALL ALL ALL

CCL-159

CCL156

DHL4 JJN-3 XG-3 PL-21 ALL-B ALL-G ALL-K NGR SCO-1 BRE HAU WMN FWL NL2 AG876 SHO JLP119 EW36 Reh UoC-B1 UoC-B3 UoC-B4 UoC-B11 SUP-B7 SUP-B13 SUP-B15 EU-1 EU-3


CRL-8286

7

Reference 1006 1006 1006 1006 1006 613 613 98 98 126 1303 1303 1303 1303 1303 1303 1303 447 447 447 447 447 447 447 1627 1627 1627 1627 1627 1627 1627 1627 1627 1627

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Melanoma (wt p53 status) Cell line A375 C32TG HS939 HS944T K2 K4 K11 K19 K23 K25 MEL-JUSO MeWo MGH-BO-1 MGH-ST-1 MGH-MC-1 MGH-QU-1 MGH-TH-1 MJM MM455 MM608 RU SK-MEL-23 SK-MEL-28 SK-MEL-30 SK-MEL-63 SK-MEL-93 SK-NEL-119 SK-MEL-147 Swift WM1205 WM115 WM239A WM35


ATCC CRL-1619

Ref 1006

CRL-7690 CRL-7693

HTB-65

HTB-72

CRL-1675

8

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Non Small Cell Lung Cancer (wt p53 status) Cell line A-549 LU99 LU99A LU99B A427 Ma-12 Ma-17 Ma-26 NCI-H460 NCI-H726 NCI-H838 NCI-H1385 NCI-H1568 NCI-H1570 NCI-H1653 NCI-H1725 NCI-H1944 NCI-H2023 NCI-H2030 NCI-H2077 NCI-H2126 NCI-H2347 NCI-H1395 NCI-H1563 NCI-H1650 NCI-H1666 NCI-H1975 NCI-H2170 NCI-H2228 NCI-H2342 NCI-H2347 NCI-H810

ATCC CCL-185

HTB-53

HTB-177 CRL-5844 CRL-5867 CRL-5876

CRL-5907 CRL-5912 CRL-5914 CRL-5919 CRL-5925 CRL-5942 CRL-5868 CRL-5875 CRL-5883 CRL-5885 CRL-5908 CRL-5928 CRL-5935 CRL-5941 CRL-5970 CRL-5816

Reference 1006 1006 1006 1006 1382 1382 1382 1382 678 678 678 678 678 678 678 678 678 678 678 678 678 678 2249 2249 678 678 2249 2249 2249 2249 2249 678

Ovarian Tumors (wt p53 status) Cell line KGN A2780 RMG-1 CH1 LK1 LK2


ATCC

9

Reference 2249 2249 2249 925 925 925

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Prostate Tumors (wt p53 status) Cell line LNCaP-FGC MDA-PCa2A PC-436C PC-82

ATCC CRL-1740

Reference 2249 1991 1991 59

RCC (wt p53 status) Cell line Caki-1

ATCC

Ref 1006

Small Cell Lung Cancer (wt p53 status) Cell line SBC-3 Lu24 MS-18 NCI-H209 NCI-H128 NCI-H1522 NCI-H2081 NCI-H446 NCI-H711 NCI-H748


ATCC

HTB-172 HTB-120 CRL-5874 CRL-5920 HTB-171 CRL-5836 CRL-5841

10

Reference 1006 1382 1382 1382 2249 2249 2249 2249 678 678

v 1.0

p53 null cell The “p53-null” status is used in different ways in the literature. The two most common meanings are a cell line with a documented p53 gene deletion (both alleles) or a cell line with a p53 mutation. We have also observed more “unusual” situations in which this status is only based on p53 expression (RNA or protein). Unfortunately, this type of information diffuses rapidly in the literature without any verification of the original publication. The p53 status of the two cell lines SK-OV-3(Ovarian cancer) and FRO (anaplastic thyroid carcinoma cell line ) are a good example of this ambiguity. In the majority of publications, the p53 status of these two cell line is stated as “p53 null”. In fact, close examination of the original manuscript shows that the p53 gene in SK-OV-3 is not deleted and did not sustain any gross rearrangement but neither p53 RNA or protein are found. In these publications, no p53 mutations were found but the recent analysis performed at the Sanger Institute detected a deletion of a single nucleotide at position 267 (codon 90) (Ikediobi et al., 2006). It is therefore possible that nonsense-mediated mRNA decay (NMD) eliminates p53 aberrant mRNA. NMD has been observed in the human leukaemia cell line K562 where p53 is also inactivated via a 1 base pair insertion at nucleotide 136. For the FRO cell line, the original reference for the analysis of the p53 gene status is always correctly quoted, but a closer look at this original paper demonstrates a marked decrease of p53 RNA in the cell but no mutation was detected by sequencing exons 5 to 8. Either a mutation is situated outside this region leading to a decrease of RNA expression (frameshift mutation associated with Nonsense-Mediated mRNA Decay) or the altered p53 expression is due to another mechanism. Because the whole p53 gene is present, it is incorrect to define SK-OV-3 or FRO cell lines as “p53 null”, as in the case of H1299 or Saos-2 cell lines in which the p53 gene is entirely deleted. These three cell lines are commonly used as recipients to reintroduce either wild-type of mutant p53. Whether the presence of an endogenous p53 gene which is still transcriptionally active in the SK-OV-3 or FRO cell could interfere with this reconstitution experiment is not known, but should be carefully considered before conducting this type of experiment. The recent finding of p53 isoforms that could be expressed by alternative splicing may also increase the complexity of this problem, as the various delta133 isoforms could be theoretically expressed in this cell line. Another reason why “p53-null” should be used cautiously to describe cell lines that express mutant p53 is the observation that p53 mutations are fairly heterogeneous in terms of loss of function and several cell lines display a normal or partial p53 response. Finally, there is now ample evidence that some mutant p53 behave as dominant oncogenes with a gain of function activity. We therefore believe that the “p53 null” status should be used only for cell lines that are totally devoid of p53 gene. Any other situation should be referred to as “mutant p53”. Cell line Hep3B* KATO III NCI-H1299 H-358 Calu-1 Saos-2 LN-Z308 HL-60 EU-4 SK-N-MC PC-3 MDA- MB-157

ATCC HB-8064 HTB-103 CRL-5803 CRL5807 HTB-54 HTB-85 CCL-240 HTB-10 HTB-24

Cancer Hepatocellular carcinoma Gastric carcinoma NSCLC NSCLC NSCLC Osteosarcoma Glioblastoma promyelocytic leukemia ALL Ewing sarcoma Prostate Breast carcinoma

reference

This table is still preliminary. More cell lines wll be added in the next version. The status of these cell lines has been verified by multiple authors Cell line p53 Handbook

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Splice Mutations HNSCC Cell line HSC-2 BICR-22 NU

ATCC

Mutation Splice intron 6 Splice intron 8 Splice intron 6

reference 105 208 105

Cell line NCI-H648 NCI-H1710 NCI-H920 NCI-H1792

ATCC CRL-5834 CRL-5850 CRL-5895

Mutation intron7 intron8 intron5 c.672+1G>A

reference 92 92 92 92

Cell line NCI-H1694 NCI-H2227 NCI-H526


Mutation c.783-1G>T c.783-2A>C splice intron3


ATCC

Mutation Intron 6

reference

NSCLC

SCLC

Endometrial cancer Cell line HEC50

This table is still preliminary. More cell lines wll be added in the next version.


12

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Cos. WT

237 330 126 164 76 365 250

ATG CTT TAC AAG GCA CAC CCC

Mut

A

Mut

Comp Name

ATA CAT TAG GAG del76 CGC CTC

Met Leu Tyr Lys Ala His Pro

Ile His Stop Glu Fs. Arg Leu

SM SM SM SM SM SM SM

WI-L2-NS WI-L2-NS EJ EJ FHS 738B1 HT-1197 HT-1376

261 AGT

del137

Ser

Fs.

DMU

271 GAG 271 GAG

AAG AAG

Glu Glu

Lys Lys

274 GTT 274 GTT

TTT TTT

Val Val

320 320 320 183 248 248 110 116 126 126 126 175 175 158 158 163 246

AAC AAC AAC TGA CAG CAG CTT TGT TAG del3a TAG CAC CAC CTC CTC TGC del1c

Lys Lys Lys Ser Arg Arg Arg Ser Tyr Tyr Tyr Arg Arg Arg Arg Tyr Met

AAG AAG AAG TCA CGG CGG CGT TCT TAC TAC TAC CGC CGC CGC CGC TAC ATG

NB

Cancer

Ref

WAF1 Comments

123 4 14 25 1 1 53

B-cell Leukemia B-cell Leukemia Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

637 769 689 729 561 561 561

0.43 9.21 NA 12.39 NA 39.94 0

J82

1

Bladder carcinoma

2249

NA

MM MM

J82 J82

38 38

Bladder carcinoma Bladder carcinoma

561 729

8.55 8.55

Phe Phe

MM MM

J82 J82

32 32


561 729

0.84 0.84

Asn Asn Asn Stop Gln Gln Leu Cys Stop InF Stop His His Leu Leu Cys Fs.

MM DMU MM DMU SM DMU SM SM SM SM DMU SM DMU DMU SM DMU SM

J82 J82 J82 RT-112 RT-112 RT-112 SD SD T-24 T-24 VM-CUB-1 VM-CUB-1 VM-CUB-1 VM-CUB-2 VM-CUB-2 VM-CUB-2 BT-483

5 5 5 29 883 883 28 3 14 1 14 1187 1187 92 92 140 1

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Breast carcinoma

561 2249 729 2249 1733 2249 294 689 689 561 2249 689 2249 689 294 689 2029

30.3 30.3 30.3 NA 0 0 12.26 10.91 NA NA NA 12.41 12.41 8.19 8.19 18.3 NA


Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report wt in COSMIC Confirmed in two other publications Controversy with other publications Deletion of exon 8 Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications v 1.0

13

Cos. WT 246 204 273 213 266

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

ATG GAG CGT CGA GGA

ATA ins7c CAT TGA GAA

Met Glu Arg Arg Gly

Ile Fs. His Stop Glu

SM SM SM SM SM

BT-483 MDA-MB-436 MDA-MB-436 DAUDI DAUDI

33 1 780 306 74

Breast carcinoma Breast carcinoma Breast carcinoma Burkitt lymphoma Burkitt lymphoma

2091 2029 1367 44 2249

0.28 NA 1.01 NA 0

158 CGC

CAC

Arg

His

SM

ST486

105

Burkitt lymphoma

44

8.78

158 CGC

CAC

Arg

His

DMU

ST486

105

Burkitt lymphoma

2249

8.78

239 AAC

GAC

Asn

Asp

DMU

ST486

53

Burkitt lymphoma

2249

20.19

234 TAC

TGC

Tyr

Cys

SM

CaR-1

133


1006

2.14

272 103 266 153 241 72 179 156 218 218 179 339 241 343

ATG del27 GAA GCC TTC del1b CGT del1a del3 del3a CGT TAG del32c TAG

Val Tyr Gly Pro Ser Pro His Arg Val Val His Glu Ser Glu

Met InF Glu Ala Phe Fs. Arg Fs. InF InF Arg Stop Fs. Stop

SM SM SM SM SM SM SM DMU SM DMU SM SM SM SM

CaR-1 COLO-205 COLO-205 HCT-15 HCT-15 KM12 KM12 RL95-2 RL95-2 RL95-2 KYSE-450 KYSE-450 KYSE-510 KYSE-510

105 1 74 5 101 1 146 3 2 3 146 12 1 5

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Endometrial tumor Endometrial tumor Endometrial tumor Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC

2051 492 1018 1018 2251 2249 1018 1625 64 1625 634 2249 634 2249

8.79 NA 0 65.41 0 NA 13.02 NA NA NA 13.02 NA NA NA

110 CGT

CTT

Arg

Leu

SM

TE-11

28

Esophageal SCC

1006

12.26

237 ATG

ATT

Met

Ile

SM

TE-11

52

Esophageal SCC

1144

0.43

251 ATC

CTC

Ile

Leu

DMU

MKN-74

5

Gastric carcinoma

1006

0

251 ATC

CTC

Ile

Leu

SM

MKN-74

5

Gastric carcinoma

94

0

GTG TAC GGA CCC TCC CCC CAT CGC GTG GTG CAT GAG TCC GAG


Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Consensus based on three puublications. Controversy with other publications Second mutation found by COSMIC Controversy with other publications. Excluded from the consensus Controversy with other publications. Excluded from the consensus Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC v 1.0

14

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

271 342 277 277 280 244 249 272 237 245 245 175 175 248 187 203 204

GAG CGA TGT TGT AGA GGC AGG GTG ATG GGC GGC CGC CGC CGG GGT GTG GAG

GCG del1a del30 del30 AGT GCC AGC ATG AAG AGC AGC CAC CAC CAG del111 GTT TAG

Glu Arg Cys Cys Arg Gly Arg Val Met Gly Gly Arg Arg Arg Gly Val Glu

Ala Fs. InF InF Ser Ala Ser Met Lys Ser Ser His His Gln InF Val Stop

DMU SM SM DMU DMU SM SM SM DMU SM DMU DMU SM DMU SM SM SM

MKN-74 SF-539 UM-SCC-14 UM-SCC-14 UM-SCC-14 HLE HLE HLE SK-LMS-1 SK-LMS-1 SK-LMS-1 SK-UT-1 SK-UT-1 SK-UT-1 EKVX EKVX EKVX

3 5 2 2 14 10 34 105 11 440 440 1187 1187 883 1 2 46

Gastric carcinoma Gliomas Head and Neck SCC Head and Neck SCC Head and Neck SCC Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

1006 2249 1019 1636 1636 1069 230 1006 2198 14 2198 2249 14 2249 1018 2249 2249

47 34 282 298 298 285 209 273 158 309 249 249 259 248 248

CCG CCC CGG GAG GAG GAG AGA CGT CGC CCC AGG AGG GAC CGG CGG

CTG ins1 CCG TAG TAG AAG TGA CAT CTC GCC AGC AGC GTC TGG CAG

Pro Pro Arg Glu Glu Glu Arg Arg Arg Pro Arg Arg Asp Arg Arg

Leu Fs. Pro Stop Stop Lys Stop His Leu Ala Ser Ser Val Trp Gln

SM DMU SM SM DMU SM SM SM SM SM SM DMU DMU SM SM

NCI-H1373 NCI-H157 NCI-H157 NCI-H157 NCI-H157 NCI-H1703 NCI-H1793 NCI-H1793 NCI-H226 NCI-H226 NCI-H324 NCI-H324 NCI-H324 PC-14 PC-14

3 1 21 71 71 165 14 780 92 1 34 34 21 728 883

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

106 1382 2021 92 1382 92 92 2249 92 1018 678 92 92 1382 2242

16.97 NA NA NA 20.55 0 12.42 8.79 14.24 0 0 12.41 12.41 0 NA NR NA 144.0 3 NA 0 NA NA 0.58 NA 1.01 8.19 55.15 12.42 12.42 10.87 0 0

280 AGA

AAA

Arg

Lys

SM

SK-MES-1

78

Lung (NSCLC)

1081

0.46


Controversy with other publications. wt in COSMIC Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications. Excluded from the cosnensus v 1.0

15

Cos. WT

298 46 273 273 258 258 317 341 342

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

GAG TCC CGT CGT GAA GAA CAG TTC CGA

TAG del1b TGT TGT AAA AAA TAG TTT TGA

Glu Ser Arg Arg Glu Glu Gln Phe Arg

Stop Fs. Cys Cys Lys Lys Stop Phe Stop

SM DMU SM DMU DMU SM DMU SM SM

SK-MES-1 NCI-H1048 NCI-H1048 NCI-H1048 MeWo MeWo MeWo MeWo MeWo

71 1 687 687 73 73 25 4 74

Lung (NSCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Melanoma Melanoma Melanoma Melanoma Melanoma

303 2249 22 2249 2249 1076 2249 2019 2019

NA NA 0.91 0.91 0.31 0.31 NA NR NA

239 AAC

GAC

Asn

Asp

SM

PA-1

53

Ovarian carcinoma

854

20.19

316 89 179 262 273

CCT del1a CGT GTT CAT

Pro Pro His Gly Arg

Pro Fs. Arg Val His

SM SM SM SM SM

PA-1 SK-OV-3 SK-OV-3 SW626 SW626

6 3 146 14 780

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

144 2249 1018 864 1011

NR NA 13.02 11.71 1.01

134 TTT

del1a

Phe

Fs.

SM

AsPC-1

4

Pancreatic cancer

397

NA

273 CGT

CAT

Arg

His

SM

ASPC-1

780

Pancreatic cancer

132

1.01

273 CGT

CAT

Arg

His

SM

Panc-1

780

Pancreatic cancer

178

1.01

273 CGT

TGT

Arg

Cys

SM

Panc-1

687

Pancreatic cancer

177

0.91

223 CCT

CTT

Pro

Leu

DMD

DU-145

5

Prostate ca.

59

8.54

274 GTT

TTT

Val

Phe

DMD

DU-145

32

Prostate ca.

59

0.84

278 CCT

GCT

Pro

Ala

DMU

786-0

24

Renal cell ca.

1018

14.87

CCC CCC CAT GGT CGT


Consensus based on three publications. Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Consensus based on four publications. Controversy with other publications Controversy with other publications. Excluded from the consensus Consensus based on four publications. Controversy with one publication Controversy with other publications. Excluded from the cosnensus Consensus based on three publications. Controvery withy other publications (only one of the two mutations is found) Consensus based on three publications. Controvery withy other publications (only one of the two mutations is found) Controversy with other publications. Splice in COSMIC v 1.0

16

Cos. WT 273 280 111 248 306

CGT AGA CTG CGG CGA

Mut

A

TGT AGT GTG CAG TGA

Arg Arg Leu Arg Arg

Mut

Comp Name

Cys Ser Val Gln Stop

SM SM SM SM SM

Rh30 RH30 MOLT-4 MOLT-4 MOLT-4

NB 687 14 1 883 160

Cancer

Ref

WAF1 Comments

Rhabdomyosarcoma Rhabdomyosarcoma T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu

1190 97 2242 27 2249

0.91 20.55 26.49 0 NA


Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications

v 1.0 17

Cos. WT

306 236 237 248 133 248 248 248 272 241 273 241 172 238 195 288 266 193 179 115 55 273 273 248 248 245 245 239 236 245 248 175 282 110

CGA TAC ATG CGG ATG CGG CGG CGG GTG TCC CGT TCC GTT TGT ATC AAT GGA CAT CAT CAT ACT CGT CGT CGG CGG GGC GGC AAC TAC GGC CGG CGC CGG CGT

Mut

A

TGA CAC GTG CAG AAG TGG TGG CAG ATG CCC CAT del1c TTT TAT ACC TAT GTA TAT CTT TAT ins1c TGT CAT TGG TGG AGC AGC ins3b TGC AGC CAG CAC TGG CCT

Arg Tyr Met Arg Met Arg Arg Arg Val Ser Arg Ser Val Cys Ile Asn Gly His His His Thr Arg Arg Arg Arg Gly Gly Asn Tyr Gly Arg Arg Arg Arg

Mut

Comp Name

Stop His Val Gln Lys Trp Trp Gln Met Pro His Fs. Phe Tyr Thr Tyr Val Tyr Leu Tyr Fs. Cys His Trp Trp Ser Ser InF Cys Ser Gln His Trp Pro

SM MM MM MM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM

2L1 CG2 CG2 CG2 HEL K051 K052 KASUMI-1 KMOE-2 ML-1 MONOMAC-1 NOMO-1 OHN-GM OHN-GM SHI-1 YSK-21 DJM-1 SW13 ASL5 440 622 8-MG-BA B2-17 CAS-1 D-336MG D-423MG D-566MG GB-1 GMS-10 KINGS-1 KINGS-1 LN-319 LN-405 MOG-G-CCM

NB

160 13 14 883 13 728 728 883 105 11 780 4 19 98 90 5 51 41 38 2 1 687 780 728 728 440 440 1 75 440 883 1187 600 11

Cancer

Ref

WAF1 Comments

Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Acute Myelogenous Leukemia Adnexal carcinoma (skin) Adrenocortical carcinoma Angiosarcoma (hepatic) Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma

2048 356 356 356 1006 243 243 2249 2249 2198 2198 2249 2049 2049 2207 1710 2249 416 1353 491 491 2249 2249 2249 2249 2249 2249 2249 2249 2249 2249 277 2249 2249

NA 14.28 13.83 0 10.08 0 0 0 8.79 0.48 1.01 NA 8.17 14.58 11.24 50.99 0 4.97 21.26 48.24 NA 0.91 1.01 0 0 0 0 NA 0.7 0 0 12.41 0.55 10.79


from patient RUPN84. Single report Single report Single report Single report wt in COSMIC Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database

v 1.0 18

Cos. WT 159 245 273 273 273 238 109 265 248 109 248 248 248 273 248 273 248 246 124 273 224 225 177 245 245 257 282 209 175 248 358 246 175 220 281

GCC GGC CGT CGT CGT TGT TTC CTG CGG TTC CGG CGG CGG CGT CGG CGT CGG ATG TGC CGT GAG GTT CCC GGC GGC CTG CGG AGA CGC CGG GAG ATG CGC TAT GAC

Mut

A

GTC AGC TGT TGT TAT GGT TCC CCG CAG TCC CAG CAG CAG CTT CAG CTT CAG ACG TGA TGT ins5 ATT TCC AGC CGC GTG ins2b ins4b TGC CAG AAG ACG CAC TGT GGC

Ala Gly Arg Arg Arg Cys Phe Leu Arg Phe Arg Arg Arg Arg Arg Arg Arg Met Cys Arg Glu Val Pro Gly Gly Leu Arg Arg Arg Arg Glu Met Arg Tyr Asp

Mut

Comp Name

Val Ser Cys Cys Tyr Gly Ser Pro Gln Ser Gln Gln Gln Leu Gln Leu Gln Thr Stop Cys Fs. Ile Ser Ser Arg Val Fs. Fs. Cys Gln Lys Thr His Cys Gly

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM MM MM MM SM SM SM SM

MOG-G-UVW no-10 no-11 SW1088 SW1783 TM-31 ALL-A ALL-C ALL-PO ALL-W EU-10 EU-11 EU-13 EU-18 EU-2 EU-6 EU-7 GR-ST HPB-ALL KARPAS-45 KG-1 KG-1a KMO-90 KOPM30 KOPN32 KOPN35 KOPN49 KOPN63 SCMC-L9 SCMC-L9 SCMC-L9 Tanoue TMBL-1 U-698-M BALL-1

NB 50 440 687 687 3 10 3 23 883 3 883 883 883 147 883 147 883 13 1 687 1 4 19 440 20 7 1 1 28 883 1 13 1187 336 16

Cancer

Ref

WAF1 Comments

Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-cell Leukemia

2249 2249 2249 2249 2249 1760 1303 1303 2249 1303 1627 1627 1627 1627 617 617 617 2249 2198 2249 126 163 239 661 661 661 661 661 478 478 478 2242 1967 2249 2242

6.91 0 0.91 0.91 NR 13.93 11.17 0 0 11.17 0 0 0 0.86 0 0.86 0 0 NA 0.91 NA 57.07 34.33 0 7.9 1.08 NA NA 61.6 0 54.45 0 12.41 1.21 12.06


Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia

Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Single report Single report Single report Mutation in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC Single report Mutation in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC Single report

database database database database database

database

database database

database

v 1.0 19

Cos. WT 248 132 285 237 330 280 127 176 248 273 274 213 234 283 216 273 158 176 238 176 245 282 273 273 175 175 271 273 266 48 280 248 162 336 241

CGG AAG GAG ATG CTT AGA TCC TGC CGG CGT GTT CGA TAC CGC GTG CGT CGC TGC TGT TGC GGC CGG CGT CGT CGC CGC GAG CGT GGA GAC AGA CGG ATC GAG TCC

Mut

A

CTG AGG AAG ATA CAT CGA TTC TCC CAG TGT CTT CAA CAC CAC ATG CAT CAC GGC CGT TTC GAC CCG CAT CAT CAC CAC AAG TGT ins1c GGT ACA CAG AAC TAG TGC

Arg Lys Glu Met Leu Arg Ser Cys Arg Arg Val Arg Tyr Arg Val Arg Arg Cys Cys Cys Gly Arg Arg Arg Arg Arg Glu Arg Gly Asp Arg Arg Ile Glu Ser

Mut

Comp Name

Leu Arg Lys Ile His Arg Phe Ser Gln Cys Leu Gln His His Met His His Gly Arg Phe Asp Pro His His His His Lys Cys Fs. Gly Thr Gln Asn Stop Cys

SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM DMU DMU DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM

HL-60(TB) KU812 RPMI-8226 WI-L2-NS WI-L2-NS 2 3 5 6 8 9 A3/KAW A3/KAW DG-75 HT HT LY1 LY1 LY17 LY2 LY7 LY8 C3 SU-DHL-1 EGI-1 ETK-1 HuCCT1 Huh-28 SNU-1196 SNU-478 SNU-869 5637 639-V 647-V 647-V BFTC-909

NB 124 51 165 123 4 1 30 13 883 687 16 38 25 19 74 780 105 7 26 191 171 21 780 780 1187 1187 38 687 1 1 92 883 4 5 36

Cancer

Ref

WAF1 Comments

B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma Biliary tract carcinoma Biliary tract carcinoma Biliary tract carcinoma Biliary tract carcinoma Biliary tract carcinoma Biliary tract carcinoma Biliary tract carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

1018 1006 1018 637 769 1098 1098 1098 1098 1098 1098 3 3 1143 2249 2249 613 613 613 613 613 613 2249 2249 2249 2249 2249 1216 1216 1216 561 689 2249 2249 564

0 14.1 0.58 0.43 9.21 NR 12.47 13.27 0 0.91 1.22 2.19 0 0.46 0.16 1.01 8.78 15.95 0.48 22.88 1.95 0 1.01 1.01 12.41 12.41 8.55 0.91 NA 62.92 0.29 0 3.56 NA 0


Single report wt in COSMIC Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Confirmed in three other publications Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC v 1.0

20

Cos. WT

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

92 14 101 105 14 25 1 1 53

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

859 689 729 2249 689 729 561 561 561

0.29 NA 0 8.78 NA 12.39 NA 39.94 0

ACA TAG TTC CAC TAG GAG del76 CGC CTC

Arg Tyr Ser Arg Tyr Lys Ala His Pro

Thr Stop Phe His Stop Glu Fs. Arg Leu

SM SM SM SM SM SM SM SM SM

BL-17 BT-1 CUBIII DSH1 EJ EJ FHS 738B1 HT-1197 HT-1376

261 AGT

del137

Ser

Fs.

DMU

J82

1

Bladder carcinoma

2249

271 GAG 271 GAG

AAG AAG

Glu Glu

Lys Lys

MM MM

J82 J82

38 38


561 729

274 GTT 274 GTT

TTT TTT

Val Val

Phe Phe

MM MM

J82 J82

32 32


561 729

320 320 320 241 213 219 36 245 280 248 248 158 183 248 248 110

AAC AAC AAC TAC TGA del1a CCA GAC AAA CAG CAG CTC TGA CAG CAG CTT

Lys Lys Lys Ser Arg Pro Pro Gly Arg Arg Arg Arg Ser Arg Arg Arg

Asn Asn Asn Tyr Stop Fs. Pro Asp Lys Gln Gln Leu Stop Gln Gln Leu

MM DMU MM SM SM SM SM SM SM SM SM SM DMU SM DMU SM

J82 J82 J82 LB831-BLC LD137 LD600 LD605 LD627 LD630 LD660 LD692 LD700 RT-112 RT-112 RT-112 SCaBER

5 5 5 19 306 10 5 171 78 883 883 92 29 883 883 28

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

561 2249 729 2249 1163 1163 1163 1163 1163 1163 1163 1163 2249 1733 2249 294

280 126 241 158 126 164 76 365 250

AGA TAC TCC CGC TAC AAG GCA CAC CCC

Mut

AAG AAG AAG TCC CGA CCC CCG GGC AGA CGG CGG CGC TCA CGG CGG CGT


Single report Single report Single report Mutation in COSMIC database Controversy with other publications Controversy with other publications Single report wt in COSMIC Confirmed in two other publications Controversy with other publications NA Deletion of exon 8 Controversy with other publications undocumented 4th mutation 8.55 (deletion 210 bp) 8.55 Controversy with other publications Controversy with other publications undocumented 4th mutation 0.84 (deletion 210 bp) 0.84 Controversy with other publications Controversy with other publications undocumented 4th mutation 30.3 (deletion 210 bp) 30.3 Controversy with other publications 30.3 Controversy with other publications 6.57 Mutation in COSMIC database NA Single report NA Single report NR Single report 1.95 Single report 0.46 Single report 0 Single report 0 Single report 8.19 Single report NA Controversy with other publications 0 Controversy with other publications 0 Controversy with other publications 12.26 Confirmed in another publication v 1.0

21

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

110 116 273 126 126 349 113 126 175 175 158 158 163 278 248 273 319 179 266

CGT TCT CGT TAC TAC GAA TTC TAC CGC CGC CGC CGC TAC CCT CGG CGT AAG CAT GGA

CTT TGT TGT TAG del3a TAA TGC TAG CAC CAC CTC CTC TGC CTT CAG TGT TAG AAT TGA

Arg Ser Arg Tyr Tyr Glu Phe Tyr Arg Arg Arg Arg Tyr Pro Arg Arg Lys His Gly

Leu Cys Cys Stop InF Stop Cys Stop His His Leu Leu Cys Leu Gln Cys Stop Asn Stop

SM SM SM SM SM SM SM DMU SM DMU DMU SM DMU SM SM SM SM SM SM

SD SD SW1710 T-24 T-24 TCCSUP UM-UC-3 VM-CUB-1 VM-CUB-1 VM-CUB-1 VM-CUB-2 VM-CUB-2 VM-CUB-2 VM-CUB-3 DB KARPAS-299 KARPAS-422 3522 S2 BRC-230

28 3 687 14 1 7 9 14 1187 1187 92 92 140 84 883 687 5 23 19

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma B-Lineage Diffuse Large Cell L B-Lineage Diffuse Large Cell L B-Lineage Diffuse Large Cell L Breast carcinoma Breast carcinoma

294 689 689 689 561 561 294 2249 689 2249 689 294 689 689 2249 2249 2249 1017 1393

12.26 10.91 0.91 NA NA NA 12.55 NA 12.41 12.41 8.19 8.19 18.3 0.81 0 0.91 NA 19.3 NA

132 285 246 246 249 224 132 280 241 248 262 281 281 248 248

AAG GAG ATG ATG AGG GAG AAG AGA TCC CGG GGT GAC GAC CGG CGG

CAG AAG del1c ATA AGC AAG GAG ACA TGC CAG GTT CAC TAC CAG CAG

Lys Glu Met Met Arg Glu Lys Arg Ser Arg Gly Asp Asp Arg Arg

Gln Lys Fs. Ile Ser Lys Glu Thr Cys Gln Val His Tyr Gln Gln

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

BT-20 BT-474 BT-483 BT-483 BT-549 CAL-148 CAL-85-1 CAMA-1 EVSA-T H-31 H-71 HCC1007 HCC1008 HCC1143 HCC1143

14 165 1 33 34 8 25 92 36 883 14 41 16 883 883

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

24 24 2029 2091 24 2249 2249 2029 2091 1689 1689 1396 2258 2249 2258

10.86 0.58 NA 0.28 12.42 38.49 0.56 0.29 0 0 11.71 0.66 7.28 0 Mutation in COSMIC database 0


Controversy with other publications Controversy with other publications Single report Controversy with other publications Controversy with other publications Single report Confirmed in two other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Confirmed in another publication. wt in COSMIC Confirmed in three other publications Controversy with other publications Controversy with other publications Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC wt in COSMIC Single report Single report Single report

v 1.0 22

Cos. WT 108 108 175 175 74 220 294 256 306 306 163 163 248 248 283 241 213 273 273 248 213 249 157 157 215 244 285 261 280 220 166 236 266 204 273

GGT GGT CGC CGC GCC TAT GAG ACA CGA CGA TAC TAC CGG CGG CGC TCC CGA CGT CGT CGG CGA AGG GTC GTC AGT GGC GAG AGT AGA TAT TCA TAC GGA GAG CGT

Mut

A

del3b del3a CAC CAC del6a TGT TAG ins2 TGA TGA TGC TGC TGG TGG TGC del1b TGA CTT CTT CAG TGA GGG TTC TTC ATT AGC AAG del26a AAA TGT TAA TGC GAA ins7c CAT

Gly Gly Arg Arg Ala Tyr Glu Thr Arg Arg Tyr Tyr Arg Arg Arg Ser Arg Arg Arg Arg Arg Arg Val Val Ser Gly Glu Ser Arg Tyr Ser Tyr Gly Glu Arg

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

InF InF His His InF Cys Stop Fs. Stop Stop Cys Cys Trp Trp Cys Fs. Stop Leu Leu Gln Stop Gly Phe Phe Ile Ser Lys Fs. Lys Cys Stop Cys Glu Fs. His

SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

1 1 1187 1187 1 336 54 2 160 160 140 140 728 728 27 26 306 147 147 883 306 52 177 177 25 72 165 1 78 336 19 75 74 1 780

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

2258 2249 1396 2258 2249 2249 1396 1396 1396 2258 2258 2249 2249 2258 1396 2258 2249 1396 2258 1396 1713 552 76 2258 1968 1393 2029 2029 24 2091 2029 2091 1018 2029 1367

NA NA 12.41 12.41 NA 1.21 NA NA NA NA 18.3 18.3 0 0 25.27 NA NA 0.86 0.86 0 NA 0.17 9.06 9.06 8.11 0.34 0.58 NA 0.46 1.21 NA 0.7 0 NA 1.01

HCC1187 HCC1187 HCC1395 HCC1395 HCC1419 HCC1419 HCC1569 HCC1806 HCC1937 HCC1937 HCC1954 HCC1954 HCC2157 HCC2157 HCC2218 HCC2713 HCC2998 HCC38 HCC38 HCC70 HDQ-P1 HMT-3909 Hs 578T HS578T L56BR-X1 MAST MDA-MB-134-VI MDA-MB-157 MDA-MB-231 MDA-MB-330 MDA-MB-361 MDA-MB-415 MDA-MB-435 MDA-MB-436 MDA-MB-436


Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Confirmed in another publication

Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Confirmed in two other publications Single report Single report wt in COSMIC wt in COSMIC Confirmed in another publication Single report wt in COSMIC Single report Single report Controversy with other publications Controversy with other publications v 1.0

23

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

368 273 132 234 244 244 277 136 273

CAC CGT AAG TAC GGC GGC TGT CAA CGT

del30 CAT AGG TAA AGC AGC TTT TAA TGT

His Arg Lys Tyr Gly Gly Cys Gln Arg

InF His Arg Stop Ser Ser Phe Stop Cys


MDA-MB-453 MDA-MB-468 MFM-223 MW1C-6.3 OCUB-F OCUB-M R11T R18T R30T

1 780 51 10 72 72 48 47 687

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

147 9 2249 147 2091 2249 1806 1806 1806

NA 1.01 14.1 NA 0.34 0.34 0.31 NA 0.91

175 161 135 237 158 144 317 265 273 213 194 342 273 190 248 206 193 273 246 237 248 176 248 282 273

CGC GCC TGC ATG CGC CAG CAG CTG CGT CGA CTT CGA CGT CCT CGG TTG CAT CGT ATG ATG CGG TGC CGG CGG CGT

CAC GAC TTC ATA ins3a TAG TAG CCG TGT TGA TTT TGA TGT del1 CAG del1 CGT TGT ACG ATA CAG TAC TGG TGG TGT

Arg Ala Cys Met Arg Gln Gln Leu Arg Arg Leu Arg Arg Pro Arg Leu His Arg Met Met Arg Cys Arg Arg Arg

His Asp Phe Ile InF Stop Stop Pro Cys Stop Phe Stop Cys Fs. Gln Fs. Arg Cys Thr Ile Gln Tyr Trp Trp Cys

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM

SK-BR-3 SK-BR-5 SUM1315MO2 SUM149PT SUM159PT SUM185PE SUM190PT SUM225CWN SUM229PE SUM52PE T47D UACC-893 Abana AKATA AKUA AS283A BJAB BL-113 BL-30 BL-37 BL-41 BL-49 BL-49 BL-60 BL-70

1187 19 52 123 1 53 25 23 687 306 28 74 687 1 883 2 86 687 13 123 883 88 728 600 687

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma

76 2091 2091 2091 2091 2091 2091 2091 2091 2091 9 2029 2247 75 447 44 75 44 75 44 75 44 44 44 2249

12.41 2.42 10.37 0.43 NA NA NA 0 0.91 NA 14.38 NA 0.91 NA 0 NA 10.15 0.91 0 0.43 0 14.82 0 0.55 0.91


wt in COSMIC Confirmed in another publication Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Confirmed in another publication. wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database

v 1.0 24

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

306 883 1187 306 74 883 2 91 13 133 14 883 883 98 98 883 26 14 687

Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma

44 447 44 44 2249 1030 44 1819 1819 44 75 1007 44 75 44 75 44 44 44

NA 0 12.41 NA 0 0 NA 9.52 0.72 2.14 10.86 0 0 14.58 14.58 0 11.22 NA 0.91

CGA CGG CGC CGA GGA CGG TTG CCG ATC TAC AAG CGG CGG TGT TGT CGG TAC GAG CGT

TGA CAG CAC TGA GAA CAG del1 CTG AAC TGC CAG CAG CAG TAT TAT CAG CAC TAG TGT

Arg Arg Arg Arg Gly Arg Leu Pro Ile Tyr Lys Arg Arg Cys Cys Arg Tyr Glu Arg

Stop Gln His Stop Glu Gln Fs. Leu Asn Cys Gln Gln Gln Tyr Tyr Gln His Stop Cys

SM SM SM SM SM SM SM DMD DMD SM SM SM SM SM SM SM DMU DMU SM

BL-99 CA46 CW678 DAUDI DAUDI DH978 EB3 GA-10 GA-10 JD38 JIYOYE KK124 KK125 LOUCKES MC116 NAMALWA P3HR1 P3HR1 PP984

213 CGA

CAA

Arg

Gln

DMD

RAJI

38

Burkitt lymphoma

161

234 TAC 254 ATC 175 CGC

CAC GAC ins3a

Tyr Ile Arg

His Asp InF

DMD SM SM

RAJI RAMOS SG568

25 3 1

Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma

161 44 447

158 CGC

CAC

Arg

His

SM

ST486

105

Burkitt lymphoma

44

158 CGC

CAC

Arg

His

DMU

ST486

105

Burkitt lymphoma

2249

239 AAC 109 TTC 273 CGT

GAC TTA TGT

Asn Phe Arg

Asp Leu Cys

DMU SM SM

ST486 C2 C33A

53 1 687

Burkitt lymphoma Cervical Cancer Cervical Cancer

2249 469 54

213 248 175 213 266 248 206 152 232 234 132 248 248 238 238 248 163 287 273


Single report Single report Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Confirmed in another publication Confirmed in another publication Single report Single report Confirmed in another publication Confirmed in another publication Confirmed in three other publications Confirmed in two other publications Confirmed in two other publications Single report Confirmed in another publication Some publications described only 2.19 one of the two mutations Confirmed in another publication Some publications described only one of the two mutations 0 NR Confirmed in three other publications NA Single report Consensus based on three puublications. Controversy with other publications Second mutation found 8.78 by COSMIC Controversy with other publications. 8.78 Excluded from the consensus Controversy with other publications. 20.19 Excluded from the consensus 9.61 Single report 0.91 Confirmed in two other publications v 1.0

25

Cos. WT

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

GTC CAC del3a CAC CAT GAT AAT ins3c CAC ins1a DEL1 CAG TAG TGG TGG del1a AGC ATG TGA AGC TAA TGA TAG

Gly Arg Asn Arg Gln Glu Lys Arg Leu Gln Pro Arg Lys Arg Arg Pro Gly Thr Arg Arg Gln Arg Glu

Val His InF His His Asp Asn InF His Fs. Fs. Gln Stop Trp Trp Fs. Ser Met Stop Ser Stop Stop Stop

SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

HT-3 IGR/Cut40 SKS CH-1 JEG-3 NUC-1 NUC-1 SCH CG3 K-562 KCL-22 KYO-1 LAMA-84 RG VACO330 ala C10 C106 C125-PM C75 C80 C84 CACO2

84 1187 7 1187 10 1 1 1 2 1 2 883 5 600 728 6 440 12 241 34 6 74 46

Cervical Cancer Cervical Cancer Cervical Cancer Chondrosarcoma choriocarcinoma choriocarcinoma choriocarcinoma choriocarcinoma Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Colorectal adenoma Colorectal adenoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

54 573 1782 1373 473 473 473 473 356 269 150 150 2249 1 1 724 2051 2051 2051 2051 2051 2051 2051

0 12.41 NA 12.41 41 49.57 74.98 NA 87.06 NA NA 0 NA 0.55 0 NA 0 14.64 NA 12.42 NA NA NA

234 TAC

TGC

Tyr

Cys

SM

CaR-1

133


1006

2.14

272 245 245 126 278 241 241 134 193 144

ATG AGC AGC TAG CAT TTC TTC TTG CGT TAG

Val Gly Gly Tyr Pro Ser Ser Phe His Gln

Met Ser Ser Stop His Phe Phe Leu Arg Stop

SM SM SM SM SM SM SM SM SM SM

CaR-1 CBS CC07 CC20 CCK-81 CLONE A CLONE D Co74 Co82 Co84

105 440 440 14 13 101 101 6 86 53

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

2051 724 2051 2051 2051 492 492 2258 2258 2258

8.79 0 0 NA 0.28 0 0 10.71 10.15 NA

245 175 131 175 167 17 24 249 289 136 301 248 319 282 248 301 245 125 196 249 52 342 204

GGC CGC AAC CGC CAG GAA AAA AGG CTC CAA CCA CGG AAG CGG CGG CCA GGC ACG CGA AGG CAA CGA GAG

Mut

GTG GGC GGC TAC CCT TCC TCC TTT CAT CAG


Confirmed in another publication Single report Single report Single report wt in COSMIC Single report Single report Single report Single report Confirmed in another publication Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Single report Single report Single report Single report Single report Single report

v 1.0 26

Cos. WT 273 196 103 266 248 321 273 248 241 176 277 245 273 306 272 300 213 153 241 273 273 213 163 72 179 234 245 175 126 241 161 273 342 224 158

CGT CGA TAC GGA CGG AAA CGT CGG TCC TGC TGT GGC CGT CGA GTG CCC CGA CCC TCC CGT CGT CGA TAC CCC CAT TAC GGC CGC TAC TCC GCC CGT CGA GAG CGC

Mut

A

TGT TGA del27 GAA TGG ins2a CAT CAG TTC TTC TTT GAC CAT TGA del2b del1a TGA GCC TTC CAT CAT CTA CAC del1b CGT CAC GAC CAC TAA TTC ACC CAT TGA GAT CTC

Arg Arg Tyr Gly Arg Lys Arg Arg Ser Cys Cys Gly Arg Arg Val Pro Arg Pro Ser Arg Arg Arg Tyr Pro His Tyr Gly Arg Tyr Ser Ala Arg Arg Glu Arg

Mut

Comp Name

Cys Stop InF Glu Trp Fs. His Gln Phe Phe Phe Asp His Stop Fs. Fs. Stop Ala Phe His His Leu His Fs. Arg His Asp His Stop Phe Thr His Stop Asp Leu

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

Co92 CoCM-1 COLO-205 COLO-205 COLO-320 COLO-741 CX-1 CX27 DLD-1 FET FRI GLY H-110 H-173 HCA46 HCA7 HCC-2998 HCT-15 HCT-15 HRA19 HT-29 HT55 ISRECO1 KM12 KM12 LIM1863 LS-1034 LS-123 LS-411 MIP 101 MOSER NCI-H508 NCI-H630 NCI-H716 NCI-H747

NB 687 241 1 74 728 1 780 883 101 191 48 171 780 160 1 8 306 5 101 780 780 33 26 1 146 25 171 1187 11 101 75 780 74 6 92

Cancer

Ref

WAF1 Comments

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

2258 2051 492 1018 38 2051 492 1 27 724 724 724 1689 1689 2051 2051 1018 1018 2251 2051 492 2051 724 2249 1018 2051 724 2051 2051 492 492 2249 2249 2051 219

0.91 NA NA 0 0 NA 1.01 0 0 22.88 0.31 1.95 1.01 NA NA NA NA 65.41 0 1.01 1.01 0.69 11.22 NA 13.02 0 1.95 12.41 NA 0 13.25 1.01 NA 59.68 8.19


Single report Controversy with other publications Controversy with other publications Confirmed in two other publications Single report Single report Single report Confirmed in three other publications Single report Single report Single report Single report Single report Single report Single report Confirmed in COSMIC database Controversy with other publications Controversy with other publications Single report Confirmed in two other publications wt in COSMIC Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Single report v 1.0

27

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

272 306 190 248 254 175 273 175 166 273 273 218 248

GTG CGA CCT CGG ATC CGC CGT CGC TCA CGT CGT GTG CGG

ATG TGA CTT TGG ACC CAC CTT CAC TGA TGT CAT TTG TGG

Val Arg Pro Arg Ile Arg Arg Arg Ser Arg Arg Val Arg

Met Stop Leu Trp Thr His Leu His Stop Cys His Leu Trp

SM SM SM SM SM SM SM SM SM DMD DMD DMD DMD

P-6 RCM-1 SNU-1033 SNU-1040 SNU-1047 SNU-1097 SNU-503 SNU-61 SNU-C1 SNU-C2B SNU-C2B SNU-C5 SNU-C5

105 160 52 728 11 1187 147 1187 18 687 780 3 728

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

1006 2051 1204 1204 1204 1204 1204 1204 2249 2051 2051 1038 1038

8.79 NA 10.12 0 0.85 12.41 0.86 12.41 NA 0.91 1.01 54.76 0

159 237 248 51

GCC ATG CGG GAA

GAC del14 CAG TAA

Ala Met Arg Glu

Asp Fs. Gln Stop

SM SM SM SM

SW1116 SW1417 SW1463 SW403

8 1 883 6

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

219 492 2249 2051

10.18 NA 0 NA

248 273 309 248 117 245 175 306 154 135 329 282 273 181 190 273 213

CGG CGT CCC CGG GGG GGC CGC CGA GGC TGC ACC CGG CGT CGC CCT CGT CGA

TGG CAT TCC TGG del1a GAC CAC TGA AGC TGG ins1a TGG TGT TGC CTT CAT CAA

Arg Arg Pro Arg Gly Gly Arg Arg Gly Cys Thr Arg Arg Arg Pro Arg Arg

Trp His Ser Trp Fs. Asp His Stop Ser Trp Fs. Trp Cys Cys Leu His Gln

SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM

SW48 SW480 SW480 SW837 SW948 V9P VACO10MS VACO429 VACO457 VACO489 VACO4A VACO5 VACO576 VACO670 VACO8 WIDR AN3

728 780 6 728 3 171 1187 160 14 25 1 600 687 28 52 780 38

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Endometrial tumor

492 9 9 9 2051 724 2051 2051 996 996 2051 996 996 996 996 27 64

0 1.01 43.57 0 NA 1.95 12.41 NA 11.47 12.8 NA 0.55 0.91 26.1 10.12 1.01 2.19


Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Confirmed in two other publications. wt in COSMIC wt in COSMIC Mutation in COSMIC database Single report Confirmed in another publication. wt in COSMIC Single report Single report Confirmed in two other publications wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report v 1.0

28

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

89 389 273 213 248 286 195 248 248 154 273 138 282 246 246

CCC GGG CGT CGA CGG GAA ATC CGG CGG GGC CGT GCC CGG ATG ATG

del1c TGG ins1a TGA CAG AAA AAC CAG CAG GAC CAT GTC TGG GTG GTG

Pro Gly Arg Arg Arg Glu Ile Arg Arg Gly Arg Ala Arg Met Met

Fs. Trp Fs. Stop Gln Lys Asn Gln Gln Asp His Val Trp Val Val

DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

AN3-CA AN3-CA EN ESS-1 HEC-116 HEC-155 HEC-180 HEC-1-A HEC-1-B HEC-251 HEC-59 HHUA HOUA IK-90 ISHIKAWA

1 1 2 306 883 86 14 883 883 9 780 48 600 57 57

Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor

2249 2249 1585 2249 1935 1935 1935 64 64 1935 1935 339 339 339 64

NA 47.89 NA NA 0 11.07 10.54 0 0 39.65 1.01 31.84 0.55 0 0

175 306 156 218 218 274 273 309 277 310 248 234 245 278 278 220 337 193 255

CGC CGA CGC GTG GTG GTT CGT CCC TGT AAC CGG TAC GGC CCT CCT TAT CGC CAT ATC

CAC TGA del1a del3 del3a GAT AGT TCC TTT ins1a TGG TGC GTC TCT TCT TGT TGC CTT GTC

Arg Arg Arg Val Val Val Arg Pro Cys Asn Arg Tyr Gly Pro Pro Tyr Arg His Ile

His Stop Fs. InF InF Asp Ser Ser Phe Fs. Trp Cys Val Ser Ser Cys Cys Leu Val

SM SM DMU SM DMU SM DMU DMU SM SM SM SM SM SM SM SM SM DMU DMU

KLE MFE-296 RL95-2 RL95-2 RL95-2 OCIM2 Bic-1 Bic-1 Flo-1 OE19 COLO-680N EC-GI-10 HCE-4 HCE-6 HCE7 KYSE- 890 KYSE-110 KYSE-1170 KYSE-1170

1187 160 3 2 3 8 20 6 48 1 728 133 84 87 87 336 19 55 4

Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Erythroleukemia Esophageal ADC Esophageal ADC Esophageal ADC Esophageal ADC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC

64 2249 1625 64 1625 12 2004 2004 2004 2249 2249 2249 5 5 1317 634 634 634 634

12.41 NA NA NA NA 1.32 17.4 43.57 0.31 NA 0 2.14 0 0.34 0.34 1.21 11.86 11.02 80.3


Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publications. wt in COSMIC Mutation in COSMIC database Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Single report Single report Single report Single report Single report Single report

v 1.0 29

Cos. WT 194 193 342 193 248 266 195 203 337 248 248 337 179 339 241 343 266 251 273 175 214 272 272 242

CTT CAT CGA CAT CGG GGA ATC GTG CGC CGG CGG CGC CAT GAG TCC GAG GGA ATC CGT CGC CAT GTG GTG TGC

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

3 55 1 86 883 20 90 2 19 728 883 19 146 12 1 5 20 1 687 1187 69 105 105 58

Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC

634 634 634 634 2249 634 2249 634 634 634 634 2249 634 2249 634 2249 634 634 634 462 1006 1006 1144 1144

95.43 11.02 NA 10.15 0 10.77 11.24 8.55 11.86 0 0 11.86 13.02 NA NA NA 10.77 NA 0.91 12.41 3.12 8.79 8.79 0

GTT CTT del7a CGT CAG CGA ACC CTG TGC TGG CAG TGC CGT TAG del32c TAG CGA ins1a TGT CAC CGT ATG ATG TAC

Leu His Arg His Arg Gly Ile Val Arg Arg Arg Arg His Glu Ser Glu Gly Ile Arg Arg His Val Val Cys

Val Leu Fs. Arg Gln Arg Thr Leu Cys Trp Gln Cys Arg Stop Fs. Stop Arg Fs. Cys His Arg Met Met Tyr

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

KYSE-1240 KYSE-1250 KYSE-1260 KYSE-140 KYSE-150 KYSE-170 KYSE-180 KYSE-190 KYSE-200 KYSE-220 KYSE-350 KYSE-410 KYSE-450 KYSE-450 KYSE-510 KYSE-510 KYSE-590 KYSE-70 KYSE-850 SK-GT-4 T.T T.Tn TE-1 TE-10

110 CGT

CTT

Arg

Leu

SM

TE-11

28

Esophageal SCC

1006

12.26

237 342 248 237 267 194 175 176 273

ATT TGA CAG ATT del1b CGT CAC TTC TGT

Met Arg Arg Met Arg Leu Arg Cys Arg

Ile Stop Gln Ile Fs. Arg His Phe Cys


TE-11 TE-15 TE-6 TE-8 TE-9 19 58 59 63

52 74 883 52 2 66 1187 191 687

Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Esophageal SCC Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma

1144 2249 1144 2249 1144 328 328 328 328

0.43 NA 0 0.43 NA 10.61 12.41 22.88 0.91

ATG CGA CGG ATG CGG CTT CGC TGC CGT


Single report Single report Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report wt in COSMIC Single report Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Mutation in COSMIC database Single report Mutation in COSMIC database wt in COSMIC Single report Single report Single report Single report v 1.0

30

Cos. WT 285 118 283 181 164 273 273 273 176 141 176 176 273 277 273 176 175 213 248 282 307 175 11 179 104 145 273 128 272 152 245 193 173 173 143

GAG ACA CGC CGC AAG CGT CGT CGT TGC TGC TGC TGC CGT TGT CGT TGC CGC CGA CGG CGG GCA CGC GAG CAT CAG CTG CGT CCT GTG CCG GGC CAT GTG GTG GTG

Mut

A

AAG ins2 DEL1C CAC GAG TGT TGT CAT TTC TAC TTC TTC TGT TAT TGT TTC CAC TGA TGG TGG del7b CAC CAG CAG TAG CGG TGT del37 ATG ins1c AGC CCT ATG GCG GCG

Glu Thr Arg Arg Lys Arg Arg Arg Cys Cys Cys Cys Arg Cys Arg Cys Arg Arg Arg Arg Ala Arg Glu His Gln Leu Arg Pro Val Pro Gly His Val Val Val

Mut

Comp Name

Lys Fs. Fs. His Glu Cys Cys His Phe Tyr Phe Phe Cys Tyr Cys Phe His Stop Trp Trp Fs. His Gln Gln Stop Arg Cys Fs. Met Fs. Ser Pro Met Ala Ala

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM

64 A673 ES-1-OT EW-13 EW-24 IARC-EW2 RD-ES RM 82 SCMC-ES-2 SCMCS-ES-1 SK-ES-1 SK-PN-DW SMB STA-ET-2.1 STA-ET-7.1 W-ES FT-MZ-1 SW684 20M AKG ECC4 G42LATE GCIY GCIY GT3TKB H-111 H-162 H-30 H-55 HGC-27 HSC-39 HUG-1N JR1 KWS MKN-1

NB 165 1 4 34 25 687 687 780 191 103 191 191 687 28 687 191 1187 306 728 600 1 1187 10 14 18 10 687 2 105 2 440 18 77 20 20

Cancer

Ref

WAF1 Comments

Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Ewing's Sarcoma Fallopian tube ca. Fibrosarcoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma

328 226 245 2249 2249 226 226 226 245 245 226 2249 226 226 226 245 590 2249 71 1393 2249 1393 2249 2249 2249 1689 1689 1689 1689 2249 2087 2242 94 94 71

0.58 NA NA 34.07 12.39 0.91 0.91 1.01 22.88 9.84 22.88 22.88 0.91 0.68 0.91 22.88 12.41 NA 0 0.55 NA 12.41 67.48 17.51 NA 12.61 0.91 NA 8.79 NA 0 9.99 10.53 7.49 11.35


Single report wt in COSMIC Single report Mutation in COSMIC database Mutation in COSMIC database Single report Confirmed in another publication Single report Single report Single report Confirmed in another publication Mutation in COSMIC database Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Confirmed in another publication v 1.0

31

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

251 ATC 278 CCT

CTC TCT

Ile Pro

Leu Ser

SM SM

MKN-28 MKN-7

5 87

Gastric carcinoma Gastric carcinoma

94 2087

0 0.34

251 ATC

CTC

Ile

Leu

DMU

MKN-74

5

Gastric carcinoma

1006

0

251 ATC

CTC

Ile

Leu

SM

MKN-74

5

Gastric carcinoma

94

0

271 248 220 342 57 175 281 205 216 266 143 273 282 215 273 381 173 175 273 88 248 242 175 132 195 267 248 105 151

GCG CAG TGT TGA del17 CAC GAG TTT ATG GAA del1 CAT TGG AAT TGT del1a ATG CAC TGT del1b CAG TTC CAC ATG ACC TGG TGG AGC TCC

Glu Arg Tyr Arg Asp Arg Asp Tyr Val Gly Val Arg Arg Ser Arg Lys Val Arg Arg Ala Arg Cys Arg Lys Ile Arg Arg Gly Pro

Ala Gln Cys Stop Fs. His Glu Phe Met Glu Fs. His Trp Asn Cys Fs. Met His Cys Fs. Gln Phe His Met Thr Trp Trp Ser Ser

DMU SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

MKN-74 NCI-N87 NUGC-3 OKAJIMA SK-GT-1 SK-GT-2 SK-GT-5 SNU-16 SNU-216 SNU-484 SNU-55 SNU-601 SNU-638 SNU-668 TGBC11TKB TGBC11TKB TMK-1 YCC-3 SJ-G2 SJ-G3 SJ-G5 A-172 A-7 D456 G-1163GM G-1187GM G123 G-1265GM G-1301M

3 883 336 74 2 1187 26 3 74 74 2 780 600 15 687 1 77 1187 687 1 883 88 1187 9 90 37 728 1 92

Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Glial tumor Glial tumor Glial tumor Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

1006 46 94 71 462 462 462 46 747 747 46 747 747 747 2249 2249 71 46 1190 1190 1190 1397 474 472 2106 2106 370 2106 2106

16.97 0 1.21 NA NA 12.41 1.66 8.34 0.16 0 NA 1.01 0.55 3.97 0.91 NA 10.53 12.41 0.91 NA 0 13.79 12.41 14.93 11.24 1.68 0 5.58 0.85

GAG CGG TAT CGA GAC CGC GAC TAT GTG GGA GTG CGT CGG AGT CGT AAA GTG CGC CGT GCC CGG TGC CGC AAG ATC CGG CGG GGC CCC


Single report Single report Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Single report Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Confirmed in another publication Single report Single report Single report Single report wt in another publication Single report Single report Single report Single report Single report Single report Single report v 1.0

32

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments 14.82 21.63 0 12.41 12.41 80.42 0.97 12.63 0 80.42 10.83 12.41 0.55 0.43 12.41 12.42 46.58 13.79 15.17 12.39 12.36 10.53 0.55 0.91 3.61 NR 15.49 1.01

176 179 249 175 175 76 220 163 248 76 161 175 282 237 175 249 250 242 238 164 197 173 282 273 173 255 255 273

TGC CAT AGG CGC CGC GCA TAT TAC CGG GCA GCC CGC CGG ATG CGC AGG CCC TGC TGT AAG GTG GTG CGG CGT GTG ATC ATC CGT

TAC GAT ATG CAC CAC GGA CAT TCC TGG GGA TCC CAC TGG ATA CAC AGT GCC TTC TCT GAG CTG ATG TGG TGT CTG ATT ATG CAT

Cys His Arg Arg Arg Ala Tyr Tyr Arg Ala Ala Arg Arg Met Arg Arg Pro Cys Cys Lys Val Val Arg Arg Val Ile Ile Arg

Tyr Asp Met His His Gly His Ser Trp Gly Ser His Trp Ile His Ser Ala Phe Ser Glu Leu Met Trp Cys Leu Ile Met His

SM SM SM SM SM DMU DMU SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM

G-210GM G-211GM G-599GM G-750GM GM133 GM1596 GM1596 GM2217 GM2313 GM2328 GM2328 GM2345 GM2493 GM47.23 GM97 GT9 GT9 LG LN-18 LN-229 LN382 LN-428 LN-428 SK-D1 SK-MG-16 SK-MG-21 SK-MG-8 SNB19

88 19 64 1187 1187 9 15 5 728 9 5 1187 600 123 1187 389 14 88 10 25 2 77 600 687 23 5 3 780

Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

2106 2106 2106 2106 1950 1950 1950 1950 1950 1950 1950 1950 1950 987 1950 1066 1066 1397 277 277 2254 277 277 698 698 698 698 632

237 213 232 242 273 273

ATG CGA ATC TGC CGT CGT

ATA CAA ACC TTC CAT TGT

Met Arg Ile Cys Arg Arg

Ile Gln Thr Phe His Cys

SM SM DMU DMU SM SM

T98G U118-MG U-138MG U-138MG U251MG V-MG-35/CE

123 38 15 88 780 687

Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

277 472 1397 1397 2254 698


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in three other publications Confirmed in two other publications. 0.43 wt in COSMIC 2.19 wt in COSMIC 1.93 Single report 13.79 Single report 1.01 Single report 0.91 Single report

v 1.0 33

Cos. WT 218 245 265 241 342 286 286 266 176 273 248 342 258 275 175 281 258 180 275 183 146 332 308 282 173 126 192 151 248 193 175 188 213 248 248

GTG GGC CTG TCC CGA GAA GAA GGA TGC CGT CGG CGA GAA TGT CGC GAC GAA GAG TGT TCA TGG ATC CTG CGG GTG TAC CAG CCC CGG CAT CGC CTG CGA CGG CGG

Mut

A

GCG AGC CCG TTC TGA AAA AAA GAA TAC CAT CAG del1a AAA TAT CAC CAC AAA del1b TGG TAA TGA del99 del19 CCG del3b del21 TAG CAC TGG CTT CAC del12 TGA CTG TGG

Val Gly Leu Ser Arg Glu Glu Gly Cys Arg Arg Arg Glu Cys Arg Asp Glu Glu Cys Ser Trp Ile Leu Arg Val Tyr Gln Pro Arg His Arg Leu Arg Arg Arg

Mut

Comp Name

Ala Ser Pro Phe Stop Lys Lys Glu Tyr His Gln Fs. Lys Tyr His His Lys Fs. Trp Stop Stop InF Fs. Pro InF InF Stop His Trp Leu His InF Stop Leu Trp

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

V-MG-6 D-542MG GAMG KALS-1 KNS-42 MO59J MO59K SF188 SF210 SF268 SF295 SF-539 SNB75 GI-1 Detroit562 TYS 4197 A253 A30 BB30-HNC BICR-16 BICR-19 BICR-22 BICR-3 BICR-31 BICR-56 BICR-6 BICR-7 Ca9-22 CAL-27 CAL-33 DOK EVSCC1 FaDu FS-1

NB 6 440 23 101 74 86 86 74 88 780 883 5 73 87 1187 41 73 1 11 3 51 1 1 21 2 4 85 33 728 55 1187 1 306 124 728

Cancer

Ref

WAF1 Comments

Glioblastoma Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliosarcoma Head and Neck Head and Neck Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

698 2249 2249 2249 2249 1471 1471 664 664 664 1018 2249 1018 2249 2249 1056 1076 2249 2032 2249 208 208 208 208 208 208 208 208 105 1854 2249 418 153 153 2252

69.33 0 0 0 NA 11.07 11.07 0 14.82 1.01 0 NA 0.31 0.42 12.41 0.66 0.31 NA 0.73 NA NA NA NA 0 NA NA NA 10.75 0 11.02 12.41 NA NA 0 0


Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Confirmed in COSMIC database Controversy with other publications Confirmed in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Confirmed in two other publications Single report v 1.0

34

Cos. WT 267 193 266 285 126 281 205 305 248 237 266 194 298 52 248 176 179 258 280 282 126 273 218 256 151 151 283 244 196 220 248 132 173 266 283

CGG CAT GGA GAG TAC GAC TAT AAG CGG ATG GGA CTT GAG CAA CGG TGC CAT GAA AGA CGG TAC CGT GTG ACA CCC CCC CGC GGC CGA TAT CGG AAG GTG GGA CGC

Mut

A

TGG CTT CGA AAG TAG GAG TGT ins1a CAG ATA GAA CGT TAG ins3a TGG TGG CGT GGA CCA TGG TGC CTT GGG del1c TCC TCC CCC AGC TGA CAT TGG GAG TTG GAA CGG

Arg His Gly Glu Tyr Asp Tyr Lys Arg Met Gly Leu Glu Gln Arg Cys His Glu Arg Arg Tyr Arg Val Thr Pro Pro Arg Gly Arg Tyr Arg Lys Val Gly Arg

Mut

Comp Name

Trp Leu Arg Lys Stop Glu Cys Fs. Gln Ile Glu Arg Stop InF Trp Trp Arg Gly Pro Trp Cys Leu Gly Fs. Ser Ser Pro Ser Stop His Trp Glu Leu Glu Arg

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM MM

GL HN HO-1-N-1 HOC313 HOC605 HOC719 HOC815 HSC-3 HSC-4 HSC-5 HSQ-89 HU 281 JSQ-3 KOSC-2 KOSC-3 KUMA-3 LB771-HNC MDA-1186 MDA-1386 MDA-1386 MDA-1483 MDA-1586 MDA-1686 MDA-1686 MDA-183 MDA-686 MKG 7 MSK-QLL1 MSK-QLL2 NA NOS-1 OC2 OC3 OM1 OSC-1

NB 37 55 20 165 14 26 117 1 883 123 74 66 71 1 728 19 146 20 1 600 17 147 11 1 92 92 35 72 241 15 728 25 68 74 5

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC

1846 2249 2249 105 105 105 105 105 105 105 2252 202 160 2249 598 2252 2249 600 600 600 2032 600 600 600 2032 600 1846 600 600 105 1784 1854 2224 105 1207

1.68 11.02 10.77 0.58 NA 1.66 8.94 NA 0 0.43 0 10.61 NA NA 0 15.16 13.02 0 NR 0.55 11.62 0.86 61.46 NA 0.85 0.85 0.18 0.34 NA 0.97 0 0.56 3.61 0 NR


Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Single report wt in COSMIC Single report Single report Single report Single report v 1.0

35

Cos. WT 299 346 280 116 176 174 150 190 95 126 54 193 256 273 309 286 336 216 258 224 209 175 273 151 258 274 249 196 271 151 274 248 171 151 224

CTG GAG AGA TCT TGC AGG ACA CCT TCT TAC TTC CAT ACA CGT CCC GAA GAG GTG GAA GAG AGA CGC CGT CCC GAA GTT AGG CGA GAG CCC GTT CGG GAG CCC GAG

Mut

A

CTA AAG ACA TCC TTC AGA GCA CCC CCT TAG TAC TAT ATA CAT ACC AAA TAG GGG AAA ins5c del2a CAC CAT TCC AAA del32c AGC TGA del1 TCC del32 TGG del46c TCC TAG

Leu Glu Arg Ser Cys Arg Thr Pro Ser Tyr Phe His Thr Arg Pro Glu Glu Val Glu Glu Arg Arg Arg Pro Glu Val Arg Arg Glu Pro Val Arg Glu Pro Glu

Mut

Comp Name

Leu Lys Thr Ser Phe Arg Ala Pro Pro Stop Tyr Tyr Ile His Thr Lys Stop Gly Lys Fs. Fs. His His Ser Lys Fs. Ser Stop Fs. Ser Fs. Trp Fs. Ser Stop

MM MM SM DMU DMU SM DMU DMU DMU DMU SM DMU DMU DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

OSC-1 OSC-1 OSC-2 OSC-3 OSC-3 OSC-4 OSC-5 OSC-5 OSC-6 OSC-6 OSC-7 OSC-8 OSC-8 OSC-9 OSC-9 PCI-13 SAS SCC-12B SCC-13 SCC-15 SCC-25 SCC-27 SCC-35 SCC-4 SCC-4451 SCC-9 SKX SQ-38 SQ9G SSC-4 SSC-9 TSU TU-139 TU-177 UD-SCC-3

NB 2 1 92 2 191 7 3 2 1 14 2 41 6 780 2 86 5 5 73 1 20 1187 780 92 73 1 34 241 2 92 1 728 1 92 10

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 2252 2249 208 208 626 208 208 160 208 1937 2249 1846 160 160 202 160 105 626 626 1636

NR 60.67 0.29 NR 22.88 NR 52.88 NR 63.47 NA 60.64 4.97 5.83 1.01 60.26 11.07 NA 2.05 0.31 NA NA 12.41 1.01 0.85 0.31 NA 12.42 NA NA 0.85 NA 0 NA 0.85 NA


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Confirmed in COSMIC database Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

36

Cos. WT 222 179 220 248 155 349 270 205 245 245 242 104 163 277 277 280 248 148 71 220 176 273 248 158 213 278 157 273 242 248 193 306 155 179 248

CCG CAT TAT CGG ACC GAA TTT TAT GGC GGC TGC CAG TAC TGT TGT AGA CGG GAT CCC TAT TGC CGT CGG CGC CGA CCT GTC CGT TGC CGG CAT CGA ACC CAT CGG

Mut

A

del13 TAT TGT CTG AAC TAA del1a CAT TGC TGC TCC TAG TGC del30 del30 AGT CTG del10 del2a TGT TTC TGT CAG CCC TGA GCT TTC CTT TTC TGG CGT TGA CCC TAT CAG

Pro His Tyr Arg Thr Glu Phe Tyr Gly Gly Cys Gln Tyr Cys Cys Arg Arg Asp Pro Tyr Cys Arg Arg Arg Arg Pro Val Arg Cys Arg His Arg Thr His Arg

Mut

Comp Name

Fs. Tyr Cys Leu Asn Stop Fs. His Cys Cys Ser Stop Cys InF InF Ser Leu Fs. Fs. Cys Phe Cys Gln Pro Stop Ala Phe Leu Phe Trp Arg Stop Pro Tyr Gln

SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

UD-SCC-4 UD-SCC-5 UD-SCC-6 UD-SCC-7 UD-SCC-8 UMB-SCC-745 UMB-SCC-864 UMB-SCC-969 UM-SCC-10A UM-SCC-10B UM-SCC-11B UM-SCC-12 UM-SCC-13 UM-SCC-14 UM-SCC-14 UM-SCC-14 UM-SCC-16 UM-SCC-19 UM-SCC-20 UM-SCC-22 UM-SCC-23 UM-SCC-27 UM-SCC-3 UM-SCC-36 UM-SCC-4 UM-SCC-46 UM-SCC-5 UM-SCC-57 UM-SCC-63 UM-SCC-68 UM-SCC-81B UPCI:SCC-103 UPCI:SCC-105 UPCI:SCC-111 UPCI:SCC-114

NB 1 128 336 124 34 7 3 12 86 86 19 18 140 2 2 14 124 2 1 336 191 687 883 21 306 24 177 147 88 728 86 160 20 128 883

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

1636 1636 1636 1636 1636 2245 2245 2245 153 153 1636 1983 1983 1019 1636 1636 153 153 1983 2245 153 2245 2245 1983 2245 1983 2248 1983 153 1983 1983 1544 1544 1544 1544

NA 13.27 1.21 0 8.59 NA NA 6.44 0 0 0 NA 18.3 NA NA 20.55 0 NA NA 1.21 22.88 0.91 0 10.17 NA 14.87 9.06 0.86 13.79 0 10.15 NA 7.8 13.27 0


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

37

Cos. WT 175 273 151 224 282 155 294 280 195 248 179 177 196 144 306 342 110 232 285 275 248 238 282 282 248 151 266 255 273 282 236 258 273 279 281

CGC CGT CCC GAG CGG ACC GAG AGA ATC CGG CAT CCC CGA CAG CGA CGA CGT ATC GAG TGT CGG TGT CGG CGG CGG CCC GGA ATC CGT CGG TAC GAA CGT GGG GAC

Mut

A

CAC CAT CAC TAG TGG CCC TAG ACA TTC CAG AAT CGC TGA TAG TGA TGA TGT AAC AAG TTT TGG TTT CCG TGG del9 CAT GAA TTC CTT TGG TAA GCA CAT GAG CAC

Arg Arg Pro Glu Arg Thr Glu Arg Ile Arg His Pro Arg Gln Arg Arg Arg Ile Glu Cys Arg Cys Arg Arg Arg Pro Gly Ile Arg Arg Tyr Glu Arg Gly Asp

Mut

Comp Name

His His His Stop Trp Pro Stop Thr Phe Gln Asn Arg Stop Stop Stop Stop Cys Asn Lys Phe Trp Phe Pro Trp InF His Glu Phe Leu Trp Stop Ala His Glu His

SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

UPCI:SCC-116 UPCI:SCC-122 UPCI:SCC-125 UPCI:SCC-136 UPCI:SCC-16 UPCI:SCC-172 UPCI:SCC-182 UPCI:SCC-29B UPCI:SCC-36 UPCI:SCC-70 UPCI:SCC-72 UPCI:SCC-99 UT-SCC-1 UT-SCC-10 UT-SCC-10 UT-SCC-12 UT-SCC-16 UT-SCC-16 UT-SCC-19 UT-SCC-2 UT-SCC-20 UT-SCC-22 UT-SCC-30 UT-SCC-33 UT-SCC-4 UT-SCC-5 UT-SCC-7 UT-SCC-8 VU1131 VU1365 YD-10B YD-15 YD-8 ZA OHR

NB 1187 780 33 10 600 20 54 92 29 883 23 19 241 53 160 74 11 13 165 47 728 42 21 600 1 3 74 37 147 600 12 3 780 42 41

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC hepatoblastoma

1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1019 1019 1019 2250 1019 1019 2250 1019 1019 1636 1019 1636 2250 1019 2250 2250 1996 1996 2189 2189 2189 105 589

12.41 1.01 10.75 NA 0.55 7.8 NA 0.29 10.47 0 19.3 17.88 NA NA NA NA 10.91 0.72 0.58 0.02 0 0.82 0 0.55 NA 10.75 0 0.48 0.86 0.55 NA 0 1.01 0.27 0.66


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

38

Cos. WT 139 249 244 249 272 244 264 220 249 249 164 161 262 200 175 282 232 156 215 237 245 245 175 175 248 282 286 251 104 163 275 212 229 278 71

AAG AGG GGC AGG GTG GGC CTA TAT AGG AGG AAG GCC GGT AAT CGC CGG ATC CGC AGT ATG GGC GGC CGC CGC CGG CGG GAA ATC CAG TAC TGT TTT TGT CCT CCC

Mut

A

GAG AGT GCC AGC ATG GCC del18 TGT AGT AGT TAG ACC GAT AAA CAC TGG del11c CCC ins1 AAG AGC AGC CAC CAC CAG TGG GCA AAC TAG TGC TTT ins39 TGA TCT del1

Lys Arg Gly Arg Val Gly Leu Tyr Arg Arg Lys Ala Gly Asn Arg Arg Ile Arg Ser Met Gly Gly Arg Arg Arg Arg Glu Ile Gln Tyr Cys Phe Cys Pro Pro

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

Glu Ser Ala Ser Met Ala InF Cys Ser Ser Stop Thr Asp Lys His Trp Fs. Pro Fs. Lys Ser Ser His His Gln Trp Ala Asn Stop Cys Phe InF Stop Ser Fs.

SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM SM SM DMU SM DMU DMU SM DMU SM SM SM SM SM SM SM SM SM SM

3 389 10 34 105 10 1 336 389 389 18 75 6 1 1187 600 1 38 3 11 440 440 1187 1187 883 600 6 21 18 140 47 1 6 87 2

Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hodgkin disease Hodgkin disease Hodgkin disease Human transformed fibroblast Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Li-Fraumeni synd. Li-Fraumeni synd. Liposarcoma Lung (?) Lung (?) Lung (?) Lung (?) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

1042 1356 1069 230 1006 230 230 230 232 38 2249 2249 2249 1042 55 55 2249 1156 1552 2198 14 2198 2249 14 2249 982 982 2249 2242 2249 2249 1018 16 1382 1976

60.6 12.42 0 12.42 8.79 0 NA 1.21 12.42 12.42 NA 13.25 11.22 22.72 12.41 0.55 NA 8.22 NA 14.24 0 0 12.41 12.41 0 0.55 0.15 8.75 NA 18.3 0.02 NA NA 0.34 NA

HCC-T HCKI-1 HLE HLE HLE HLF HuH-4 HuH-7 Malhavu PLC/PRF/5 SNU-387 SNU-449 SNU-475 TONG/HCC CO CO HD-MY-Z HH4dd LMS6-93 SK-LMS-1 SK-LMS-1 SK-LMS-1 SK-UT-1 SK-UT-1 SK-UT-1 LBC282 LBC286 SW872 AOI BEN ChaGo-K-1 HOP-62 866MT ABC-1 ACC-LC-319


Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Confirmed in two other publications Single report wt in another publication Single report Confirmed in two other publications Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in another publication Single report Single report Mutation in COSMIC database Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report v 1.0

39

Cos. WT 281 209 135 280 237 196 175 171 187 203 204 273 248 349 139 154 234 175 132 249 237 241 216 248 272 11 273 248 248 245 282 175 337 237 121

GAC AGA TGC AGA ATG CGA CGC GAG GGT GTG GAG CGT CGG GAA AAG GGC TAC CGC AAG AGG ATG TCC GTG CGG GTG GAG CGT CGG CGG GGC CGG CGC CGC ATG TCT

Mut

A

GGC TGA TTC AAA ATT TGA CAC TAG del111 GTT TAG TGT CTG TAA del1c GTC TGC CTC AAT AGT ATT TGC TTG CTG ATG CAG CAT TGG CAG GTC TGG CAC TGC ATA del1c

Asp Arg Cys Arg Met Arg Arg Glu Gly Val Glu Arg Arg Glu Lys Gly Tyr Arg Lys Arg Met Ser Val Arg Val Glu Arg Arg Arg Gly Arg Arg Arg Met Ser

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

Gly Stop Phe Lys Ile Stop His Stop InF Val Stop Cys Leu Stop Fs. Val Cys Leu Asn Ser Ile Cys Leu Leu Met Gln His Trp Gln Val Trp His Cys Ile Fs.

SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

16 14 52 78 52 241 1187 25 1 2 46 687 124 7 1 65 133 25 23 389 52 36 13 124 105 10 780 728 883 84 600 1187 19 123 1

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

1976 1393 2249 1081 2249 16 1382 1382 1018 2249 2249 1386 1386 1386 1327 1327 1327 1018 2195 2249 364 2249 2249 1382 1006 1382 1081 2249 1382 1382 1574 1382 1382 1382 1382

12.06 NA 10.37 0.46 0.43 NA 12.41 NA NA NR NA 0.91 0 NA NA 8.39 2.14 13.16 10.49 12.42 0.43 0 2.73 0 8.79 67.48 1.01 0 0 0 0.55 12.41 11.86 0.43 NA

ACC-LC94 CAEP CAL-12T Ca-Lu-1 CALU-3 CALU6 CMRC-LCD EBC-1 EKVX EKVX EKVX GLCA2 GLCP1 GLCP1 HCC1438 HCC2108 HCC2279 HOP-92 IGR-Heu KNS-62 LC-1-SQ LC-2-ad LCLC-103H LCMS LK-2 LU-65 LX-1 LXF-289 MA1 MA-10 MA14 MA2 MA-24 MA25 MA-29


Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Confirmed in COSMIC database Single report Mutation in COSMIC database Confirmed in another publication Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Confirmed in another publication Single report Single report Confirmed in another publication Single report Confirmed in another publication Single report v 1.0

40

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments 0 0 1.01 NA NA 0 2.14 59.68 0.58 144.0 3 NA 6.4 0 NA NA 0 NA NA 0 NA 0.86 12.41 NA 14.82 0.58 0.86 13.79 NR 0 9.06 NA 1.01 0.86 0.43

245 245 273 239 298 248 234 224 285

GGC GGC CGT AAC GAG CGG TAC GAG GAG

TGC GTC CAT ins1b TAG TGG TGC GAC AAG

Gly Gly Arg Asn Glu Arg Tyr Glu Glu

Cys Val His Fs. Stop Trp Cys Asp Lys


MA3 MA36 NCI-H1155 NCI-H125 NCI-H1264 NCI-H1284 NCI-H1304 NCI-H1334 NCI-H1355

86 84 780 1 71 728 133 10 165

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

1382 1574 92 678 92 678 678 92 92

47 144 141 267 89 34 282 298 298 248 144 273 175 35 176 285 273 242 247 248 157 209 273 273 237

CCG CAG TGC CGG CCC CCC CGG GAG GAG CGG CAG CGT CGC TTG TGC GAG CGT TGC AAC CGG GTC AGA CGT CGT ATG

CTG TAG TGG CCG del1 ins1 CCG TAG TAG CTG TAG CTT CAC ins1b TAC AAG CTT TTC AAT TGG TTC TGA CAT CTT ATA

Pro Gln Cys Arg Pro Pro Arg Glu Glu Arg Gln Arg Arg Leu Cys Glu Arg Cys Asn Arg Val Arg Arg Arg Met

Leu Stop Trp Pro Fs. Fs. Pro Stop Stop Leu Stop Leu His Fs. Tyr Lys Leu Phe Asn Trp Phe Stop His Leu Ile

SM SM SM SM SM DMU SM SM DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

NCI-H1373 NCI-H1404 NCI-H1435 NCI-H1437 NCI-H1466 NCI-H157 NCI-H157 NCI-H157 NCI-H157 NCI-H1573 NCI-H1581 NCI-H1623 NCI-H1628 NCI-H1648 NCI-H1651 NCI-H1703 NCI-H1734 NCI-H1755 NCI-H1770 NCI-H1770 NCI-H1781 NCI-H1793 NCI-H1793 NCI-H1838 NCI-H1869

3 53 11 19 3 1 21 71 71 124 53 147 1187 1 88 165 147 88 39 728 177 14 780 147 123

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

106 92 92 92 92 1382 2021 92 1382 92 92 92 678 92 92 92 92 92 2249 2249 92 92 2249 2249 678


Confirmed in another publication Single report Confirmed in two other publications Single report Single report Single report wt in COSMIC Single report Confirmed in another publication Controversy with other publications Single report Single report Confirmed in another publication Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report wt in COSMIC Single report Single report wt in COSMIC Single report Controversy with other publications wt in COSMIC Single report Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Mutation in COSMIC database Single report

v 1.0 41

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments NA NA 14.19 0.86 11.71 14.19 0 9.06 0 71.32 22.88 NA 13.25 8.19 55.15 8.39 0.28 1.01 0 12.42 12.42 10.87

209 291 242 273 262 242 220 157 248 16 176 62 161 158 309 154 246 273 248 249 249 259

AGA AAG TGC CGT GGT TGC TAT GTC CGG CAG TGC GAA GCC CGC CCC GGC ATG CGT CGG AGG AGG GAC

TGA TAG TGG CTT GTT TGG TCT TTC TGG CTG TTC TAA ACC CTC GCC GTC ATC CAT CTG AGC AGC GTC

Arg Lys Cys Arg Gly Cys Tyr Val Arg Gln Cys Glu Ala Arg Pro Gly Met Arg Arg Arg Arg Asp

Stop Stop Trp Leu Val Trp Ser Phe Trp Leu Phe Stop Thr Leu Ala Val Ile His Leu Ser Ser Val

SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM DMU DMU

NCI-H1904 NCI-H1915 NCI-H1993 NCI-H2009 NCI-H2030 NCI-H2073 NCI-H2086 NCI-H2087 NCI-H2106 NCI-H2122 NCI-H2122 NCI-H2126 NCI-H2250 NCI-H226 NCI-H226 NCI-H2291 NCI-H23 NCI-H2405 NCI-H322 NCI-H324 NCI-H324 NCI-H324

14 9 16 147 14 16 20 177 728 1 191 8 75 92 1 65 6 780 124 34 34 21

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

92 92 2249 92 2249 92 106 92 106 2249 2249 2249 92 92 1018 2249 17 2249 92 678 92 92

158 146 191 245 164 215 217 248 102 176 162 267

CGC TGG CCT GGC AAG AGT GTG CGG ACC TGC ATC CGG

CTC TGA del1a TGC AAT ATT TTG CTG del1 TGG ins9c CCG

Arg Trp Pro Gly Lys Ser Val Arg Thr Cys Ile Arg

Leu Stop Fs. Cys Asn Ile Leu Leu Fs. Trp InF Pro

SM SM SM SM SM SM DMU DMU SM SM SM SM

NCI-H441 NCI-H520 NCI-H522 NCI-H596 NCI-H650 NCI-H661 NCI-H676 NCI-H676 NCI-H679 NCI-H720 NCI-H727 NCI-H738

92 51 8 86 8 25 3 124 1 19 1 19

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

92 92 92 92 92 92 92 92 92 92 1707 678


Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Mutation in COSMIC database Confirmed in another publication Mutation in COSMIC database Confirmed in another publication Controversy with other publications Controversy with other publications Controversy with other publications Confirmed in another publication. wt 8.19 in COSMIC NA wt in COSMIC NA Single report 0 Confirmed in another publication 22.27 Single report 8.11 wt in COSMIC 50.18 Single report 0 Single report NA Single report 15.16 wt in COSMIC NA Single report 0 Single report

v 1.0 42

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

ACA GAG AGG GGG GGC GGG CGG CGG CGG CAT CGG CAG GGC TTC CGC CAT

CCA AAG AGT GTG TGC GTG TGG CAG TGG CGT CAG TAG TGC TGC CTC CGT

Thr Glu Arg Gly Gly Gly Arg Arg Arg His Arg Gln Gly Phe Arg His

Pro Lys Ser Val Cys Val Trp Gln Trp Arg Gln Stop Cys Cys Leu Arg

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

NCI-H820 NCI-H854 OG56 PC-1 PC-10 PC-13 PC-14 PC-14 PC-3 PC-7 PC-9 PERF-LC-AI RAL RERF-LOCK SK-LC-6 SKLU1

10 165 389 3 86 3 728 883 600 69 883 18 53 9 92 86

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

106 92 1976 1382 364 1382 1382 2242 364 1382 364 1976 1393 1382 1976 16

7.25 0.58 12.42 25.23 0 25.23 0 0 0.55 3.12 0 NA 0 12.55 8.19 10.15

280 AGA

AAA

Arg

Lys

SM

SK-MES-1

78

Lung (NSCLC)

1081

0.46

298 277 167 347 175 175 204 157 220 126 249 110 152 245 306 281

TAG TTT TAG ACC del1 CAC TAG TTC TGT TGC AGT del1 ins1 TGC TGA CAC

Glu Cys Gln Ala Arg Arg Glu Val Tyr Tyr Arg Arg Pro Gly Arg Asp

Stop Phe Stop Thr Fs. His Stop Phe Cys Cys Ser Fs. Fs. Cys Stop His


SK-MES-1 SW1271 SW900 U-1752 U-1810 VRMC-LCD Y-ML-1B NCI-H2066 NCI-H2286 ACC-LC-172 ACC-LC-48 ACC-LC-49 ACC-LC-80 COLO-668 COR-L51 COR-L96CAR

71 48 40 3 3 1187 46 177 336 17 389 3 8 86 160 41

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (other) Lung (other) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

303 16 2249 2021 2021 1976 1867 92 92 1976 1976 1976 1976 2249 2249 2249

NA 0.31 NA 24.06 NA 12.41 NA 9.06 1.21 11.62 12.42 NA NA 0 NA 0.66

284 285 249 334 245 334 248 248 282 214 248 104 244 113 158 193

GAG TGT CAG GCC CGC CGC GAG GTC TAT TAC AGG CGT CCG GGC CGA GAC


Single report Single report Single report Single report Single report Single report Controversy with other publications Controversy with other publications Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Controversy with other publications. Excluded from the cosnensus Consensus based on three publications. Controversy with other publications Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database v 1.0

43

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

245 155 245 241

GGC ACC GGC TCC

GTC CCC TGC TTC

Gly Thr Gly Ser

Val Pro Cys Phe

SM SM SM SM

CPC-N DMS-153 DMS-273 DMS-53

84 20 86 101

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

2249 1953 2249 2249

0 7.8 0 0

278 237 273 282 53 146 175 158 132 332 326 218 144 280 193 294 294 278 278 244 248 157 342 179 156 244 298 298 46 273

CCT ATG CGT CGG TGG TGG CGC CGC AAG ATC GAA GTG CAG AGA CAT GAG GAG CCT CCT GGC CGG GTC CGA CAT CGC GGC GAG GAG TCC CGT

del2c ATA CTT TGG TGA TAG CAC CTC GAG del1c TAA GGG CCG GGA CTT TAG del1a CTT CTT TGC CTG TTC del1b TAT del13c AGC TAG TAG del1b TGT

Pro Met Arg Arg Trp Trp Arg Arg Lys Ile Glu Val Gln Arg His Glu Glu Pro Pro Gly Arg Val Arg His Arg Gly Glu Glu Ser Arg

Fs. Ile Leu Trp Stop Stop His Leu Glu Fs. Stop Gly Pro Gly Leu Stop Fs. Leu Leu Cys Leu Phe Fs. Tyr Fs. Ser Stop Stop Fs. Cys

SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU SM

DMS-79 DMS-92 GLC14 GLC28 GLC3 GLC35 GLC35 GLC36 GLC4 GLC42 GLC44 GLC45 GLC7 GLC8 HOV IST-SL2 LB647-SCLC LU-130 LU-134-A LU-135 LU-138 LU-139 LU-140 LU-141 LU-165 MS-1 N230 N231 NCI-H1048 NCI-H1048

1 123 147 600 7 46 1187 92 25 4 9 11 10 40 55 54 1 84 84 53 124 177 1 128 1 72 71 71 1 687

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

2249 16 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 2249 2249 1382 364 364 1382 364 1382 1382 2249 2242 1382 1382 2249 22

NA 0.43 0.86 0.55 NA NA 12.41 8.19 0.56 NA NA 61.46 13.75 21.11 11.02 NA NA 0.81 0.81 0 0 9.06 NA 13.27 NA 0.34 NA NA NA 0.91


Mutation in COSMIC database wt in COSMIC Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database. del2 and ins 1 Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Controversy with other publications Controversy with other publications

v 1.0 44

Cos. WT 273 249 334 175 179 194 318 68 151 248 266 241 273 273 213 245 147 214 220 248 209 144 157 134 65 277 236 282 283 171 248 342 242 242 298

CGT AGG GGG CGC CAT CTT CCA GAG CCC CGG GGA TCC CGT CGT CGA GGC GTT CAT TAT CGG AGA CAG GTC TTT AGA TGT TAC CGG CGC GAG CGG CGA TGC TGC GAG

Mut

A

TGT AGT GTG del1b CAG CGT del19b TAG CAC CTG GTA TGC CTT CTT TGA CGC GAT CGT GAT CAG TGA TAG TTC TTA TGA TTT TGC GGG CCC TAG CAG TGA TCC TCC TAG

Arg Arg Gly Arg His Leu Pro Glu Pro Arg Gly Ser Arg Arg Arg Gly Val His Tyr Arg Arg Gln Val Phe Arg Cys Tyr Arg Arg Glu Arg Arg Cys Cys Glu

Mut

Comp Name

Cys Ser Val Fs. Gln Arg Fs. Stop His Leu Val Cys Leu Leu Stop Arg Asp Arg Asp Gln Stop Stop Phe Leu Stop Phe Cys Gly Pro Stop Gln Stop Ser Ser Stop

DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

NCI-H1048 NCI-H1105 NCI-H1184 NCI-H1417 NCI-H1436 NCI-H1450 NCI-H146 NCI-H1514 NCI-H1607 NCI-H1618 NCI-H1672 NCI-H187 NCI-H1881 NCI-H1882 NCI-H1926 NCI-H1930 NCI-H1963 NCI-H1963 NCI-H2029 NCI-H211 NCI-H2141 NCI-H2171 NCI-H2196 NCI-H231 NCI-H2330 NCI-H250 NCI-H345 NCI-H510 NCI-H64 NCI-H69 NCI-H719 NCI-H774 NCI-H841 NCI-H889 NCI-N417

NB 687 389 3 2 14 66 1 5 33 124 51 36 147 147 306 20 7 69 5 883 14 53 177 4 9 48 75 48 35 25 883 74 19 19 71

Cancer

Ref

WAF1 Comments

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

2249 2249 22 678 678 17 2249 22 22 22 22 1712 22 678 22 2249 22 22 2249 22 22 2249 2249 9 2249 2249 2249 22 2249 47 2249 22 1382 17 106

0.91 12.42 25.23 NA 17.51 10.61 NA NA 10.75 0 0 0 0.86 0.86 NA 7.9 11.64 3.12 0.96 0 NA NA 9.06 10.71 NA 0.31 0.7 0.44 0.18 NA 0 NA 0 0 NA


Controversy with other publications Mutation in COSMIC database Single report wt in COSMIC wt in COSMIC Single report Mutation in COSMIC database Single report Single report wt in COSMIC Single report Single report Single report wt in COSMIC wt in COSMIC Mutation in COSMIC database wt in COSMIC wt in COSMIC Mutation in COSMIC database Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Mutation in COSMIC database Confirmed in three other publications Mutation in COSMIC database Confirmed in another publication Single report Confirmed in another publication Single report v 1.0

45

Cos. WT

Mut

A

Mut

Comp Name

298 GAG 120 AAG

TAG TAG

Glu Lys

Stop SM Stop SM

RERF-LCMA SBC-1

248 278 85 259 248 273 215 175 245 245 278 147 242 275 247 248 246 236 258 278 234 187 127 175 127 213 238 274 249 248 193 135

CTG CGT del1 GTC CTG TGT TGT CAC GAC AGC TCT GGT TTC GGT ACC TGG AGG CAC AAA TCT TGC AGT TGC CAC TTC CAA TAT TTT GGG TGG CGT CGC

Arg Pro Pro Asp Arg Arg Ser Arg Gly Gly Pro Val Cys Cys Asn Arg Met Tyr Glu Pro Tyr Gly Ser Arg Ser Arg Cys Val Arg Arg His Cys

Leu Arg Fs. Val Leu Cys Cys His Asp Ser Ser Gly Phe Gly Thr Trp Arg His Lys Ser Cys Ser Cys His Phe Gln Tyr Phe Gly Trp Arg Arg

SBC-5 SCLC-21H SK-LC-2 U-1690 U-1906 RPMI-8402 DEL A D JMN M15 Mino Daoy PFSK-1 8823 8823 136-2 1402P 14362M 16396M 17697M 20842P 2211M 30966M 4686M 518A2 607B A2058 A4-FUK BE CHL-1 COLO-800

CGG CCT CCT GAC CGG CGT AGT CGC GGC GGC CCT GTT TGC TGT AAC CGG ATG TAC GAA CCT TAC GGT TCC CGC TCC CGA TGT GTT AGG CGG CAT TGC

SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

NB 71 2 124 39 1 21 124 687 6 1187 171 440 87 8 88 11 6 728 13 13 73 87 133 12 3 1187 30 38 98 32 52 728 86 11

Cancer

Ref

Lung (SCLC) Lung (SCLC)

364 2249

NA NA

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lymphoid leukemia Malignant histiocytosis Malignant Mesothelioma Malignant Mesothelioma Malignant Mesothelioma Malignant Mesothelioma Mantle Cell Lymphoma Meduloblastoma Meduloblastoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

364 2249 1976 2021 2021 163 2249 69 69 138 138 1842 73 2249 1158 1158 1158 1927 1927 1927 1927 1927 1927 1927 1927 1158 1158 2249 2249 2068 2249 2249

0 0.72 NA 10.87 0 0.91 25.48 12.41 1.95 0 0.34 10.49 13.79 0.62 0.12 0 2.86 14.28 0.31 0.34 2.14 51.53 5.32 12.41 12.47 2.19 14.58 0.84 0.17 0 10.15 0.86


WAF1 Comments Confirmed in another publication Mutation in COSMIC database Confirmed in another publication. wt in COSMIC Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Confirmed in two other publications Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database

v 1.0 46

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments 0.72 0.92 NA NA 54.76 0 0 0.31 0.31 NA NR NA 21.11 0 67.3 18.3 0.48 NA NR 69.34 13.83 51.83 111.2 5 90.77 85.98 NA NA 0 12.61 0.31 1.68 1.01 NA 53.77

278 268 229 213 247 248 266 258 258 317 341 342 280 248 290 163 241 166 75 76 104 155

CCT AAC TGT CGA AAC CGG GGA GAA GAA CAG TTC CGA AGA CGG CGC TAC TCC TCA CCT GCA CAG ACC

CGT ATC del2b TGA AAA TGG GAA AAA AAA TAG TTT TGA GGA CAG CAC TGC CCC TGA CCC ACA CAC AGC

Pro Asn Cys Arg Asn Arg Gly Glu Glu Gln Phe Arg Arg Arg Arg Tyr Ser Ser Pro Ala Gln Thr

Arg Ile Fs. Stop Lys Trp Glu Lys Lys Stop Phe Stop Gly Gln His Cys Pro Stop Pro Thr His Ser

SM SM SM SM SM SM SM DMU SM DMU SM SM SM SM SM SM SM SM MM MM MM MM

DX-3 HMV-II IGR39D IPC-298 IST-MEL1 IST-MEL1 M14 MeWo MeWo MeWo MeWo MeWo MLMA MM229 MM386 MRI-H-221 NZM4 RPMI-7951 SK-MEL-110 SK-MEL-110 SK-MEL-110 SK-MEL-110

39 2 6 306 4 728 74 73 73 25 4 74 40 883 31 140 11 18 1 2 2 5

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

347 2249 1158 2249 2249 2249 1018 2249 1076 2249 2019 2019 2249 1158 1158 1023 1638 1570 2019 2019 2019 2019

289 296 61 104 125 245 145 258 267 273 283 340

CTC CAC GAT CAG ACG GGC CTG GAA CGG CGT CGC ATG

TTC TAC GTT TAG del2b AGC CGG AAA TGG CAT del2c ATA

Leu His Asp Gln Thr Gly Leu Glu Arg Arg Arg Met

Phe Tyr Val Stop Fs. Ser Arg Lys Trp His Fs. Ile

MM MM SM SM SM SM SM SM SM DMU DMU SM

SK-MEL-110 SK-MEL-110 SK-MEL-136 SK-MEL-146 SK-MEL-149 SK-MEL-2 SK-MEL-28 SK-MEL-29 SK-MEL-3 SK-MEL-30 SK-MEL-30 SK-MEL-37

5 7 1 18 1 440 10 73 37 780 1 1

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

2019 2019 2019 2019 2019 1018 1018 2019 2249 2249 2249 2019


Single report Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report

v 1.0 47

Cos. WT 295 195 177 220 241 278 241 278 272 248 248 255 248 175 251

CCT ATC CCC TAT TCC CCT TCC CCT GTG CGG CGG ATC CGG CGC ATC

174 AGG 172 GTT 261 273 132 143 337 261 184 286 285 161 126 176 181 282 280 280

AGT CGT AAG GTG CGC AGT GAT GAA GAG GCC TAC TGC CGC CGG AGA AGA

Mut

A

GCT ACC del18c TGT TTC TTT TTT TCT GAG CAG CAG del6b CAG CAC DEL1B DEL26 A DEL46 A DEL99 A CAT AAC ATG CTC ACT TAT AAA AAG ACC AAC TAC TGC TGG ACA ACA

Mut

Comp Name

Pro Ile Pro Tyr Ser Pro Ser Pro Val Arg Arg Ile Arg Arg Ile

Ala Thr InF Cys Phe Phe Phe Ser Glu Gln Gln InF Gln His Fs.

SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM

SK-MEL-93/4 UISO-MEL-11 UISO-MEL-23 WM164 WM852 WM983 MCC13 MCC13 MCC14/2 BT MA18 MEF-1 SKM-1 KY821 TF-1

Arg

Fs.

SM

THP-1

Val

Fs.

SM

U937

Ser Arg Lys Val Arg Ser Asp Glu Glu Ala Tyr Cys Arg Arg Arg Arg

InF His Asn Met Leu Thr Tyr Lys Lys Thr Asn Tyr Cys Trp Thr Thr


UT7 ARH-77 EJM HD-70 KMS-12-PE L-363 LB 831 LP-1 RPMI-8226 U266 XG-1 XG-2 XG-4 XG-5 CNE-1 CNE-2

NB

Cancer

Ref

WAF1 Comments

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Merkel cell carcinoma Merkel cell carcinoma Merkel cell carcinoma Mesothelioma Mesothelioma Multiple Myeloma Myelodysplastic synd. Myeloid leukemia Myeloid leukemia

2019 2020 2020 233 233 233 2088 2088 2088 1892 1574 1363 1363 126 126

91.58 11.24 NA 1.21 0 NR 0 0.34 11.52 0 0 NA 0 12.41 NA

1

Myeloid leukemia

126

NA

Confirmed in another publication

2

Myeloid leukemia

126

NA

Single report

Myeloid leukemia Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Nasopharyngeal carcinoma Nasopharyngeal carcinoma

126 2249 98 1004 2249 2249 98 2249 98 98 98 98 98 98 108 108

NA 1.01 10.49 10.31 10.45 96.18 58.13 11.07 0.58 13.25 12.03 14.82 26.1 0.55 0.29 0.29

1 90 1 336 101 9 6 87 12 883 883 2 883 1187 5

1 780 33 34 9 1 5 86 165 75 6 88 28 600 92 92


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report wt in COSMIC Single report

Single report Mutation in COSMIC database Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Confirmed in two other publications Confirmed in another publication v 1.0

48

Cos. WT 130 113 342 286 286 182 173 175 177 176 245 176 135 110 246 282 233 238 248 282 237 237 248 248 156 306 76 286 250 245 99 316 273 172 193

CTC TTC CGA GAA GAA TGC GTG CGC CCC TGC GGC TGC TGC CGT ATG CGG CAC TGT CGG CGG ATG ATG CGG CGG CGC CGA GCA GAA CCC GGC TCC CCC CGT GTT CAT

Mut

A

GTC TCC CTA AAA AAA TGA ATG CAC ACC TTC AGC TTC TTC CTT AGG del3a ins4b CGT TGG TGG ATA ATA CAG CAG CCC TGA ins1a AAA del8 AGC del2b CCT CAT TTT CTT

Leu Phe Arg Glu Glu Cys Val Arg Pro Cys Gly Cys Cys Arg Met Arg His Cys Arg Arg Met Met Arg Arg Arg Arg Ala Glu Pro Gly Ser Pro Arg Val His

Mut

Comp Name

Val Ser Leu Lys Lys Stop Met His Thr Phe Ser Phe Phe Leu Arg InF Fs. Arg Trp Trp Ile Ile Gln Gln Pro Stop Fs. Lys Fs. Ser Fs. Pro His Phe Leu

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM SM SM SM SM SM SM SM SM SM SM SM

NPC/HK1 ACN CHLA-119 CHLA-172 CHLA-90 LAN1 NB13 NB16 NB19 NB-SD NMB SJNB-4 SK-N-BE(2) SK-N-DZ SK-N-FI TGW CHP-100 HA1 KHYG-1 AHH-1 WTK1 OCI-Ly4 OCI-Ly4 WSU-NHL HOS HOSM-2 HuO-3N1 OH RIT-1B RIT2 6 222 2774 2780CP A. P.

NB 22 3 1 86 86 6 77 1187 1 191 440 191 52 28 13 1 1 26 728 600 123 123 883 883 38 160 1 86 1 440 1 6 780 19 55

Cancer

Ref

WAF1 Comments

Nasopharyngeal carcinoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroepithelioma Ni(II) NK-Leukemia None None Non-Hodgkin's Lymphomas Non-Hodgkin's Lymphomas Non-Hodgkin's Lymphomas Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

145 2249 1774 1774 1774 93 2249 2249 2249 1190 1772 1772 1772 2249 2249 2096 93 83 1125 1127 879 517 517 2249 25 1920 2249 272 65 65 376 798 798 963 1011

8.89 12.37 65.63 11.07 11.07 NA 10.53 12.41 18.58 22.88 0 22.88 10.37 12.26 2.86 NA NA 0.48 0 0.55 0.43 0.43 0 0 8.22 NA NA 11.07 NA 0 NA NR 1.01 8.17 11.02


Single report Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Confirmed in another publication Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

49

Cos. WT

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

TTT TGA CGT TAA GAT TGA CGC TGT del32 ACC CGT TGA TGG TGC TTA TGG TAC del21a CAT CGT TGC TTT CAG GTC ins3 del18

Val Arg His Gln Val Arg Cys Arg Val Ile Leu Arg Arg Tyr Phe Arg Asp Tyr Arg Ser Tyr Cys Arg Leu Glu Tyr

Phe Stop Arg Stop Asp Stop Arg Cys Fs. Thr Arg Stop Trp Cys Leu Trp Tyr InF His Arg Cys Phe Gln Val InF InF

SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM SM SM SM SM SM SM SM SM SM

A2780-CP-20 C. P./1 C. V. Caov-3 Caov-4 DDL11 EFO-21 EFO-27 EG F. P. G. C. G. M. GBM IGROV-1 IGROV-1/Pt IGROV-1/Pt KURAMOCHI NCI/ADR-RES OC-314 OV 90 OV1P OVCA 432 OVCAR-3 OVCAR-4 OVCAR-5 OVCAR-8

19 241 86 47 7 306 4 687 1 90 66 160 600 17 7 600 16 6 780 4 17 48 883 22 1 1

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

798 1011 1011 144 144 1011 2249 2249 798 1011 1011 1011 1011 2249 606 606 144 983 2249 1165 925 1011 144 2249 864 864

8.17 NA 10.15 NA 11.64 NA 8.62 0.91 NA 11.24 10.61 NA 0.55 11.62 8.14 0.55 7.28 NA 1.01 1.17 11.62 0.31 0 8.89 NA NA

239 AAC

GAC

Asn

Asp

SM

PA-1

53

Ovarian carcinoma

854

20.19

316 195 275 273 89 179 262

CCT ACC TAT CAT del1a CGT GTT

Pro Ile Cys Arg Pro His Gly

Pro Thr Tyr His Fs. Arg Val


PA-1 PM1015 R. B. S. P. SK-OV-3 SK-OV-3 SW626

6 90 87 780 3 146 14

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

144 798 1011 1011 2249 1018 864

NR 11.24 0.42 1.01 NA 13.02 11.71

172 196 193 136 147 213 124 273 143 195 194 306 282 126 270 282 281 126 273 215 126 277 248 130 224 126

GTT CGA CAT CAA GTT CGA TGC CGT GTG ATC CTT CGA CGG TAC TTT CGG GAC TAC CGT AGT TAC TGT CGG CTC GAG TAC

Mut

CCC ATC TGT CGT CCC CAT GGT


Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report wt in two other publications Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report wt in another publication wt in COSMIC Single report Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications v 1.0

50

Cos. WT

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

780 1187 1187 17 92

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Pancreatic cancer Pancreatic cancer

1011 1165 2249 428 428

1.01 12.41 12.41 13.27 0.85

CAT CAC CAC AGC TCC

Arg Arg Arg Cys Pro

His His His Ser Ser

SM SM SM SM SM

SW626 TOV 112D TYK-nu 8902 8988S

134 TTT

del1a

Phe

Fs.

SM

AsPC-1

4

Pancreatic cancer

397

NA

273 CGT 197 GTG 275 TGT

CAT TTG TAT

Arg Val Cys

His Leu Tyr

SM SM SM

ASPC-1 BI BJ

780 5 87

Pancreatic cancer Pancreatic cancer Pancreatic cancer

132 1653 1653

1.01 12.36 0.42

220 TAT

TGT

Tyr

Cys

SM

BxPC-3

336

Pancreatic cancer

176

1.21

159 273 242 175 272 262 151 266 193 152 249 181 282 255 130 306 132 273 248 209

GTC CAT CGC CAC TTG GTT TCC GAA CGT del1a ATG CAC TGG ACC GTC TGA AGG TGT TGG del2b

Ala Arg Cys Arg Val Gly Pro Gly His Pro Arg Arg Arg Ile Leu Arg Lys Arg Arg Arg

Val His Arg His Leu Val Ser Glu Arg Fs. Met His Trp Thr Val Stop Arg Cys Trp Fs.

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

CAPAN-1 capan-2 CFPAC-1 FAMPAC Ger H-74 HPAF-II HPC-Y19 HPC-Y21 HPC-YO Hs 700T HS766T HuP-T3 HuP-T4 IMIM-PC-1 IMIM-PC-2 MCC1 MDA-Panc3 MIA PaCa-2 MZ1-PC

50 780 14 1187 39 14 92 74 86 3 64 34 600 19 22 160 51 687 728 14

Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer

177 132 397 2116 1653 1689 177 440 440 440 132 132 2249 2249 177 177 2000 1653 177 2249

6.91 1.01 0 12.41 7.06 11.71 0.85 0 10.15 NA 0 34.07 0.55 11.89 8.89 NA 14.1 0.91 0 NA

273 175 175 176 151

CGT CGC CGC TGC CCC

Mut

GCC CGT TGC CGC GTG GGT CCC GGA CAT CCG AGG CGC CGG ATC CTC CGA AAG CGT CGG AGA


Controversy with other publications Single report Mutation in COSMIC database Single report Single report Consensus based on four publications. Controversy with other publications Controversy with other publications. Excluded from the consensus Single report Single report Consensus based on six publications. A single publication decsribes a second mutation (neutral mutation) Confirmed in two other publications. wt in COSMIC wt in COSMIC Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Confirmed in four other publications Mutation in COSMIC database v 1.0

51

Cos. WT

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

TGG TGG GGG TGT

Arg Cys Arg Tyr

Trp Trp Gly Cys

SM SM SM SM

MZ-PC-2 MZ-PC-4 PAN-03-JCK Panc 89

600 25 48 336

Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer

177 177 1556 176

0.55 12.8 0.44 1.21

273 CGT

CAT

Arg

His

SM

Panc-1

780

Pancreatic cancer

178

1.01

273 255 176 175 176 237 237 167 213 255 132 280 175 282 179 175 238 245 273 220 273 179 215 273 138 331 196 273

TGT AAC AGC CAC AGC ATA ATA TAG TGA ACC CAG AAA CAC TGG CGT CAC TAT AGC CAT TGT CAT CGT del3a CAT del1 CGG TGA CAT

Arg Ile Cys Arg Cys Met Met Gln Arg Ile Lys Arg Arg Arg His Arg Cys Gly Arg Tyr Arg His Ser Arg Ala Gln Arg Arg

Cys Asn Ser His Ser Ile Ile Stop Stop Thr Gln Lys His Trp Arg His Tyr Ser His Cys His Arg InF His Fs. Arg Stop His

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU

Panc-1 PANC-10-05 Panc-TU-I PC PC-44 PCI-55 PK-1 PK-8 PK-9 PL 45 PSN1 PT45 RWP-2 SK-PC-1 SK-PC-3 SNU-213 SNU-494 SU86.86 Suit-2 T3M4 UK Pan-1 YAPC MHH-ES-1 HS-Sch-2 1LN 22Rv1 ARCaP CWR-R1

687 6 17 1187 17 123 123 40 306 19 14 78 1187 600 146 1187 98 440 780 336 780 146 1 780 5 3 241 780

Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pelvic carcinoma Peripheral nerve Sheath Tumors Prostate ca. Prostate ca. Prostate ca. Prostate ca.

177 2249 176 1653 428 1556 1556 1556 1556 965 38 178 177 177 177 1491 1491 397 1653 1653 659 2249 2249 2078 220 1991 1991 1991

0.91 6.97 13.27 12.41 13.27 0.43 0.43 NA NA 11.89 10.86 0.46 12.41 0.55 13.02 12.41 14.58 0 1.01 1.21 1.01 13.02 NA 1.01 NA 67.76 NA 1.01

282 135 282 220

CGG TGC CGG TAT

Mut

CGT ATC TGC CGC TGC ATG ATG CAG CGA ATC AAG AGA CGC CGG CAT CGC TGT GGC CGT TAT CGT CAT AGT CGT GCC CAG CGA CGT


Single report Single report Single report Single report Consensus based on four publications. Controversy with one publication Controversy with other publications. Excluded from the cosnensus Mutation in COSMIC database Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report wt in COSMIC Single report Single report v 1.0

52

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments 67.76 Single report Consensus based on three publications. Controvery withy other publications (only one of the two 8.54 mutations is found) Consensus based on three publications. Controvery withy other publications (only one of the two 0.84 mutations is found) 0 Single report NA Single report 12.41 Single report NR Confirmed in another publication NR Single report 1.01 Single report NA Single report NA Confirmed in two other publications 0.27 Single report NA Single report 0 Single report 0.42 Single report 18.22 Single report 1.01 Single report 67.3 Single report 22.88 Single report NA Single report 2.64 Single report 2.64 Single report 0.42 Single report NA Single report 1.01 Single report 0 Single report 0.42 Single report NA Single report Controversy with other publications. 14.87 Splice in COSMIC NA Mutation in COSMIC database

331 CAG

CGG

Gln

Arg

DMU

CWR-R1

3

Prostate ca.

1991

223 CCT

CTT

Pro

Leu

DMD

DU-145

5

Prostate ca.

59

274 248 138 175 152 152 273 196 138 279 126 248 276 306 273 290 176 249 173 173 275 316 273 248 275 294

GTT CGG GCC CGC CCG CCG CGT CGA GCC GGG TAC CGG GCC CGA CGT CGC TGC AGG GTG GTG TGT CCC CGT CGG TGT GAG

TTT TGG del1 CAC CCA CCA CAT TGA del1 GAG TAG TGG GAC CAA CAT CAC TTC del1 GGG GGG TAT del1 CAT CAG TAT TAG

Val Arg Ala Arg Pro Pro Arg Arg Ala Gly Tyr Arg Ala Arg Arg Arg Cys Arg Val Val Cys Pro Arg Arg Cys Glu

Phe Trp Fs. His Pro Pro His Stop Fs. Glu Stop Trp Asp Gln His His Phe Fs. Gly Gly Tyr Fs. His Gln Tyr Stop

DMD SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

DU-145 DuCaP Dupro LAPC-4 LNCaP-ATCC LNCaP-GW LNCaP-GW MDA-PCa1 PC-3 PSK-1 TSU VCaP 1 2 4 6 9 107 110 111 121 123 124 154 121LN 122LN

278 CCT 258 GAA

GCT TAA

Pro Glu

Ala DMU Stop SM

786-0 A704

32 728 5 1187 13 13 780 241 5 42 14 728 12 1 780 31 191 6 15 15 87 1 780 883 87 54

Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca.

59 1991 220 1991 316 316 316 1991 59 1991 59 1991 1564 1564 1564 1564 1564 214 214 214 214 214 214 214 214 214

24 29

Renal cell ca. Renal cell ca.

1018 2249


v 1.0 53

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

176 260 175 336 219 264 252 98 219

TGC TCC CGC GAG CCC CTA CTC CCT CCC

TTC ins2a CAC TAG del1a CGA del9 TCT del4

Cys Ser Arg Glu Pro Leu Leu Pro Pro

Phe Fs. His Stop Fs. Arg InF Ser Fs.


KRC/Y RCC23 RXF393 SN12C SNU-267 TK10 VMRC-RCZ WERI-Rb-1 ctr

191 1 1187 5 10 8 4 4 1

Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Renal cell ca. Retinoblastoma Rhabdomyosarcoma

635 468 1018 1018 1404 1018 1006 2249 97

22.88 NA 12.41 NA NA 0.8 NA 4.84 NA

248 273 280 156 316 317 326 179 281 282 132 175 248

CGG CGT AGA CGC CCC CAG GAA CAT GAC CGG AAG CGC CGG

TGG TGT AGT CCC CCT TAG TAA TAT GAT TGG AAT CAC CAG

Arg Arg Arg Arg Pro Gln Glu His Asp Arg Lys Arg Arg

Trp Cys Ser Pro Pro Stop Stop Tyr Asp Trp Asn His Gln

SM SM SM SM SM SM SM DMD DMD DMD SM DMD DMD

728 687 14 38 6 25 9 128 29 600 23 1187 883

Rhabdomyosarcoma Rhabdomyosarcoma Rhabdomyosarcoma Rhabdomyosarcoma Sarcoma Sarcoma Sarcoma Skin Keratinocytes (immortalized) Skin Keratinocytes (immortalized) Skin Keratinocytes (immortalized) Spindle Cell SCC T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu

14 1190 97 14 2249 2249 2249 244 244 244 2231 3 3

0 0.91 20.55 8.22 NR NA NA 13.27 NR 0.55 10.49 12.41 0

37 TCC

CCC

Ser

Pro

MM

1

T-cell Acute Lymphoblastic Leu

1703

65.94 Single report

175 CGC

CAC

Arg

His

MM

1187


1703

12.41 Single report

248 196 360 196 256 259 260 272

CAG TGA del1a TGA GCA GGC GCC ATG

Arg Arg Gly Arg Thr Asp Ser Val

Gln Stop Fs. Stop Ala Gly Ala Met

MM DMU DMU MM MM MM MM SM

RD Rh30 RH30 RMS GCT GCT S-117 HaCaT HaCaT HaCaT SCSC CCRF-CEM CCRF-CEM CCRF-CEMVLB100 CCRF-CEMVLB100 CCRF-CEMVLB100 J-RT3-T3-5 J-RT3-T3-5 JURKAT JURKAT JURKAT JURKAT Loucy

883 241 2 241 9 6 4 105

T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu

1703 2249 2249 3 3 3 3 1510

0 NA NA NA 10.79 17.27 66.25 8.79

CGG CGA GGG CGA ACA GAC TCC GTG


Single report Single report Confirmed in COSMIC database Confirmed in COSMIC database Single report Confirmed in COSMIC database wt in COSMIC Mutation in COSMIC database Single report Confirmed in another publication. wt in COSMIC Controversy with other publications Controversy with other publications Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Confirmed in two other publications Confirmed in two other publications

Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report v 1.0

54

Cos. WT 273 237 244 111 248 306 11 248 248 273 248 267 273 278 175 176 196 272 273 248 273 259 251 248 161 255 273 152 245 273 192 266 273 273 223

CGT ATG GGC CTG CGG CGA GAG CGG CGG CGT CGG CGG CGT CCT CGC TGC CGA GTG CGT CGG CGT GAC ATC CGG GCC ATC CGT CCG GGC CGT CAG GGA CGT CGT CCT

Mut

A

CAT AGG TGC GTG CAG TGA CAG CCG CAG TGT CAG CTG CAT TCT GGC TAC TGA del1a CAT GGG CAT TAC ACC CAG GAC AGC CAT CTG AGC CAT TAG GTA CAT CAT CTT

Arg Met Gly Leu Arg Arg Glu Arg Arg Arg Arg Arg Arg Pro Arg Cys Arg Val Arg Arg Arg Asp Ile Arg Ala Ile Arg Pro Gly Arg Gln Gly Arg Arg Pro

Mut

Comp Name

His Arg Cys Val Gln Stop Gln Pro Gln Cys Gln Leu His Ser Gly Tyr Stop Fs. His Gly His Tyr Thr Gln Asp Ser His Leu Ser His Stop Val His His Leu

SM DMU DMU SM SM SM MM MM MM SM MM MM MM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

MOLT-13 MOLT-16 MOLT-16 MOLT-4 MOLT-4 MOLT-4 P12-ICHIKAWA P12-ICHIKAWA P12-ICHIKAWA PF-382 SUP-T1 SUP-T1 SUP-T1 ATL1K HATL MT1 HUT78 NCCIT 8305C 8505C ARO BCPAP BHT-101 C 643 CAL-62 CGTH-W-1 FTC-133 HTC-C3 Hth 7 KAT 4 KOA2 NPA PTC-UC 2.2 PTC-UC 4.1 RO82-W-1

NB 780 8 53 1 883 160 10 23 883 687 883 1 780 87 27 88 241 3 780 22 780 33 7 883 19 7 780 91 440 780 85 51 780 780 5

Cancer

Ref

WAF1 Comments

T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell leukemia T-cell leukemia T-cell leukemia T-cell Lymphoma Testicular ca. Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma

2249 3 3 2242 27 2249 2249 2249 2249 2249 2249 2249 2249 82 323 82 3 999 1006 155 188 2249 2249 2160 2249 2249 84 155 2160 2160 1049 188 780 780 2249

1.01 5.71 0 26.49 0 NA 67.48 6.51 0 0.91 0 3.41 1.01 0.34 10.52 14.82 NA NA 1.01 0 1.01 9.92 3.27 0 2.42 5.46 1.01 9.52 0 1.01 NA 0 1.01 1.01 8.54


Mutation in COSMIC database wt in COSMIC wt in COSMIC Controversy with other publications Controversy with other publications Controversy with other publications Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database v 1.0

55

Cos. WT 255 379 223 135 287 162 183 273 151 266 248 171 273 314 151 155 245 249 248 245

ATC CGC CCT TGC GAG ATC TCA CGT CCC GGA CGG GAG CGT TCC CCC ACC GGC AGG CGG GGC

Mut

A

AGC TGC CTT TTC del1a ATG TGA CAT CAC GTA CAG TAG TGT TCT CAC ATC AGC del1b CAG AGC

Ile Arg Pro Cys Glu Ile Ser Arg Pro Gly Arg Glu Arg Ser Pro Thr Gly Arg Arg Gly

Mut

Comp Name

Ser Cys Leu Phe Fs. Met Stop His His Val Gln Stop Cys Ser His Ile Ser Fs. Gln Ser

SM SM SM SM SM DMU DMU SM SM SM SM SM DMU DMU SM SM DMU DMU SM SM

SW579 TCO-1 WRO A388 UPT-1 UPT-29 UPT-29 A-431 CAL-39 SW962 UM-SCA-1 UM-SCV-2 UM-SCV-3 UM-SCV-3 UM-SCV-4 UM-SCV-5 UM-SCV-7 UM-SCV-7 UT-SCV-1 SK-NEP-1

NB 7 1 5 52 2 7 29 780 33 51 883 25 687 2 33 23 440 26 883 440

Cancer

Ref

WAF1 Comments

Thyroid Carcinoma Thyroid Carcinoma Thyroid Carcinoma Unknown Primary Tumor Unknown Primary Tumor Unknown Primary Tumor Unknown Primary Tumor Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Wilm's tumor

155 2249 188 2249 314 314 314 153 2249 2249 1625 681 681 681 681 681 681 681 1625 2249

5.46 47.81 8.54 10.37 NA 31.54 NA 1.01 10.75 0 0 NA 0.91 NR 10.75 8.85 0 NA 0 0


Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Confirmed in two other publications Mutation in COSMIC database Mutation in COSMIC database Single report Confirmed in another publication Confirmed in another publication Confirmed in another publication Confirmed in another publication Single report Confirmed in another publication Confirmed in another publication Single report Mutation in COSMIC database

v 1.0 56

Bladder Tumors Table I : cell lines with wt p53 Cell line BC16 HU456 HT1197* KK47 RT4 PSI* RT112 TCCSUP UCRU-BL13 UCRU-BL-28 MGH-U UM-UC2 UM-UC11 UM-UC6

ATCC

Reference 729 729 729 729 561 729 729 729

mdm2 overexpression Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) No data


57

v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 280 248 162 336 241 280 126 241 158 126 164 76 365 250

AGA CGG ATC GAG TCC AGA TAC TCC CGC TAC AAG GCA CAC CCC

Cancer

Ref

WAF1 Comments

92 883 4 5 36 92 14 101 105 14 25 1 1 53

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

561 689 2249 2249 564 859 689 729 2249 689 729 561 561 561

0.29 0 3.56 NA 0 0.29 NA 0 8.78 NA 12.39 NA 39.94 0

ACA CAG AAC TAG TGC ACA TAG TTC CAC TAG GAG del76 CGC CTC

Arg Arg Ile Glu Ser Arg Tyr Ser Arg Tyr Lys Ala His Pro

Thr Gln Asn Stop Cys Thr Stop Phe His Stop Glu Fs. Arg Leu

SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM

5637 639-V 647-V 647-V BFTC-909 BL-17 BT-1 CUBIII DSH1 EJ EJ FHS 738B1 HT-1197 HT-1376

261 AGT

del137

Ser

Fs.

DMU

J82

1

Bladder carcinoma

2249

NA

271 GAG 271 GAG

AAG AAG

Glu Glu

Lys Lys

MM MM

J82 J82

38 38


561 729

8.55 8.55

274 GTT 274 GTT

TTT TTT

Val Val

Phe Phe

MM MM

J82 J82

32 32


561 729

0.84 0.84

320 320 320 241 213 219 36 245 280 248 248

AAC AAC AAC TAC TGA del1a CCA GAC AAA CAG CAG

Lys Lys Lys Ser Arg Pro Pro Gly Arg Arg Arg

Asn Asn Asn Tyr Stop Fs. Pro Asp Lys Gln Gln

MM DMU MM SM SM SM SM SM SM SM SM

J82 J82 J82 LB831-BLC LD137 LD600 LD605 LD627 LD630 LD660 LD692

5 5 5 19 306 10 5 171 78 883 883

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

561 2249 729 2249 1163 1163 1163 1163 1163 1163 1163

30.3 30.3 30.3 6.57 NA NA NR 1.95 0.46 0 0

AAG AAG AAG TCC CGA CCC CCG GGC AGA CGG CGG


58

Confirmed in three other publications Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Single report Single report Single report Mutation in COSMIC database Controversy with other publications Controversy with other publications Single report wt in COSMIC Confirmed in two other publications Controversy with other publications Deletion of exon 8 Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications undocumented 4th mutation (deletion 210 bp) Controversy with other publications Controversy with other publications Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 158 183 248 248 110 110 116 273 126 126 349 113 126 175 175 158 158 163 278

CGC TCA CGG CGG CGT CGT TCT CGT TAC TAC GAA TTC TAC CGC CGC CGC CGC TAC CCT

CTC TGA CAG CAG CTT CTT TGT TGT TAG del3a TAA TGC TAG CAC CAC CTC CTC TGC CTT


Arg Ser Arg Arg Arg Arg Ser Arg Tyr Tyr Glu Phe Tyr Arg Arg Arg Arg Tyr Pro

Leu Stop Gln Gln Leu Leu Cys Cys Stop InF Stop Cys Stop His His Leu Leu Cys Leu

SM DMU SM DMU SM SM SM SM SM SM SM SM DMU SM DMU DMU SM DMU SM

LD700 RT-112 RT-112 RT-112 SCaBER SD SD SW1710 T-24 T-24 TCCSUP UM-UC-3 VM-CUB-1 VM-CUB-1 VM-CUB-1 VM-CUB-2 VM-CUB-2 VM-CUB-2 VM-CUB-3

92 29 883 883 28 28 3 687 14 1 7 9 14 1187 1187 92 92 140 84

Cancer

Ref

WAF1 Comments

Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma Bladder carcinoma

1163 2249 1733 2249 294 294 689 689 689 561 561 294 2249 689 2249 689 294 689 689

8.19 NA 0 0 12.26 12.26 10.91 0.91 NA NA NA 12.55 NA 12.41 12.41 8.19 8.19 18.3 0.81

59

Single report Controversy with other publications Controversy with other publications Controversy with other publications Confirmed in another publication Controversy with other publications Controversy with other publications Single report Controversy with other publications Controversy with other publications Single report Confirmed in two other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report

v 1.0

Brain Tumors Table I : cell lines with wt p53 Cell line CHP-134* KG-1-C GOTO IMR-32* LAN-5* NB-1 NH-12 NH-6 SK-N-AS* SK-N-SH* U-87-MG GM2300 GM1578 GM2455 GM1600 GM1592 GM139 GM2401 SK-MG-11 SK-MG-15 V-MG-33

ATCC

CCL-127

CRL-2137 HTB-11 HTB-14

Origin Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma

Reference

Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

2249 1950 1950 1950 1950 1950 1950 1950 698 698 698

1006 1006

1006 1006 1006

* Cytoplasmic p53 Table II : cell lines with p53 gene deletion or rearrangement Cell line LN-Z308

ATCC

Origin Glioblastoma

Reference

Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV)


60

v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB

Cancer

Ref

WAF1 Comments

115 55 273 273 248 248 245 245 239 236 245 248 175 282 110 159 245 273 273 273 238

CAT ACT CGT CGT CGG CGG GGC GGC AAC TAC GGC CGG CGC CGG CGT GCC GGC CGT CGT CGT TGT

TAT ins1c TGT CAT TGG TGG AGC AGC ins3b TGC AGC CAG CAC TGG CCT GTC AGC TGT TGT TAT GGT

His Thr Arg Arg Arg Arg Gly Gly Asn Tyr Gly Arg Arg Arg Arg Ala Gly Arg Arg Arg Cys

Tyr Fs. Cys His Trp Trp Ser Ser InF Cys Ser Gln His Trp Pro Val Ser Cys Cys Tyr Gly

SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM

440 622 8-MG-BA B2-17 CAS-1 D-336MG D-423MG D-566MG GB-1 GMS-10 KINGS-1 KINGS-1 LN-319 LN-405 MOG-G-CCM MOG-G-UVW no-10 no-11 SW1088 SW1783 TM-31

2 1 687 780 728 728 440 440 1 75 440 883 1187 600 11 50 440 687 687 3 10

Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma Astrocytoma

491 491 2249 2249 2249 2249 2249 2249 2249 2249 2249 2249 277 2249 2249 2249 2249 2249 2249 2249 1760

48.24 NA 0.91 1.01 0 0 0 0 NA 0.7 0 0 12.41 0.55 10.79 6.91 0 0.91 0.91 NR 13.93

Single report Single report Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Single report Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Mutation in COSMIC Single report

273 88 248 242 175 132 195 267 248 105 151 176

CGT GCC CGG TGC CGC AAG ATC CGG CGG GGC CCC TGC

TGT del1b CAG TTC CAC ATG ACC TGG TGG AGC TCC TAC

Arg Ala Arg Cys Arg Lys Ile Arg Arg Gly Pro Cys

Cys Fs. Gln Phe His Met Thr Trp Trp Ser Ser Tyr

SM SM SM SM SM SM SM SM SM SM SM SM

SJ-G2 SJ-G3 SJ-G5 A-172 A-7 D456 G-1163GM G-1187GM G123 G-1265GM G-1301M G-210GM

687 1 883 88 1187 9 90 37 728 1 92 88

Glial tumor Glial tumor Glial tumor Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

1190 1190 1190 1397 474 472 2106 2106 370 2106 2106 2106

0.91 NA 0 13.79 12.41 14.93 11.24 1.68 0 5.58 0.85 14.82

Single report Single report Single report wt in another publication Single report Single report Single report Single report Single report Single report Single report Single report


61

database database database database database database database database database database database database database database database database database

v 1.0


Cancer

Ref

WAF1 Comments 21.63 0 12.41 12.41 80.42 0.97 12.63 0 80.42 10.83 12.41 0.55 0.43 12.41 12.42 46.58 13.79 15.17 12.39 12.36 10.53 0.55 0.91 3.61 NR 15.49 1.01

179 249 175 175 76 220 163 248 76 161 175 282 237 175 249 250 242 238 164 197 173 282 273 173 255 255 273

CAT AGG CGC CGC GCA TAT TAC CGG GCA GCC CGC CGG ATG CGC AGG CCC TGC TGT AAG GTG GTG CGG CGT GTG ATC ATC CGT

GAT ATG CAC CAC GGA CAT TCC TGG GGA TCC CAC TGG ATA CAC AGT GCC TTC TCT GAG CTG ATG TGG TGT CTG ATT ATG CAT

His Arg Arg Arg Ala Tyr Tyr Arg Ala Ala Arg Arg Met Arg Arg Pro Cys Cys Lys Val Val Arg Arg Val Ile Ile Arg

Asp Met His His Gly His Ser Trp Gly Ser His Trp Ile His Ser Ala Phe Ser Glu Leu Met Trp Cys Leu Ile Met His

SM SM SM SM DMU DMU SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM

G-211GM G-599GM G-750GM GM133 GM1596 GM1596 GM2217 GM2313 GM2328 GM2328 GM2345 GM2493 GM47.23 GM97 GT9 GT9 LG LN-18 LN-229 LN382 LN-428 LN-428 SK-D1 SK-MG-16 SK-MG-21 SK-MG-8 SNB19

19 64 1187 1187 9 15 5 728 9 5 1187 600 123 1187 389 14 88 10 25 2 77 600 687 23 5 3 780

Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

2106 2106 2106 1950 1950 1950 1950 1950 1950 1950 1950 1950 987 1950 1066 1066 1397 277 277 2254 277 277 698 698 698 698 632

237 213 232 242 273 273 218

ATG CGA ATC TGC CGT CGT GTG

ATA CAA ACC TTC CAT TGT GCG

Met Arg Ile Cys Arg Arg Val

Ile Gln Thr Phe His Cys Ala

SM SM DMU DMU SM SM SM

T98G U118-MG U-138MG U-138MG U251MG V-MG-35/CE V-MG-6

123 38 15 88 780 687 6

Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma Glioblastoma

277 472 1397 1397 2254 698 698


62

Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in three other publications Confirmed in two other publications. 0.43 wt in COSMIC 2.19 wt in COSMIC 1.93 Single report 13.79 Single report 1.01 Single report 0.91 Single report 69.33 Single report

v 1.0


Cancer

Ref

WAF1 Comments

245 265 241 342 286 286 266 176 273 248 342 258 275

GGC CTG TCC CGA GAA GAA GGA TGC CGT CGG CGA GAA TGT

AGC CCG TTC TGA AAA AAA GAA TAC CAT CAG del1a AAA TAT

Gly Leu Ser Arg Glu Glu Gly Cys Arg Arg Arg Glu Cys

Ser Pro Phe Stop Lys Lys Glu Tyr His Gln Fs. Lys Tyr

SM SM SM SM SM SM SM SM SM SM SM SM SM

D-542MG GAMG KALS-1 KNS-42 MO59J MO59K SF188 SF210 SF268 SF295 SF-539 SNB75 GI-1

440 23 101 74 86 86 74 88 780 883 5 73 87

Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliomas Gliosarcoma

2249 2249 2249 2249 1471 1471 664 664 664 1018 2249 1018 2249

0 0 0 NA 11.07 11.07 0 14.82 1.01 0 NA 0.31 0.42

Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Confirmed in COSMIC database Controversy with other publications Confirmed in COSMIC database Mutation in COSMIC database

113 342 286 286 182 173 175 177 176 245 176 135 110 246 282 233

TTC CGA GAA GAA TGC GTG CGC CCC TGC GGC TGC TGC CGT ATG CGG CAC

TCC CTA AAA AAA TGA ATG CAC ACC TTC AGC TTC TTC CTT AGG del3a ins4b

Phe Arg Glu Glu Cys Val Arg Pro Cys Gly Cys Cys Arg Met Arg His

Ser Leu Lys Lys Stop Met His Thr Phe Ser Phe Phe Leu Arg InF Fs.


ACN CHLA-119 CHLA-172 CHLA-90 LAN1 NB13 NB16 NB19 NB-SD NMB SJNB-4 SK-N-BE(2) SK-N-DZ SK-N-FI TGW CHP-100

3 1 86 86 6 77 1187 1 191 440 191 52 28 13 1 1

Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroblastoma Neuroepithelioma

2249 1774 1774 1774 93 2249 2249 2249 1190 1772 1772 1772 2249 2249 2096 93

12.37 65.63 11.07 11.07 NA 10.53 12.41 18.58 22.88 0 22.88 10.37 12.26 2.86 NA NA

Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report

CAT

Arg

His

SM

HS-Sch-2

780

Peripheral nerve Sheath Tumors

2078

1.01

273 CGT


63

Single report

v 1.0

Breast Tumors Table I : cell lines with wt p53 Cell line MCF-7 MCF10-2A DU4475 MDA-MB175VII MPE600 SK-BR-7 SUM102PT UACC-812 ZR75-1 ZR75-30 HBL-100* MRK-NU-1 YMB-1 YMB-1-E

ATCC HTB-22

Reference 2091

HTB-123

2091

HTB-25

2091 2091 2091 2091 2091 2091 2091 2029 1006 1006 1006

CRL-1897 CRL-1500 CRL-1504

*Positive for SV40

Table II : cell lines with p53 gene deletion or rearrangement Cell line MDA- MB-157

ATCC HTB-24

Reference

Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) no data


64

v 1.0

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

WAF1 Comments

179 CAT 266 GGA

AAT TGA

His Gly

Asn SM Stop SM

3522 S2 BRC-230

23 19

Breast carcinoma Breast carcinoma

1017 1393

19.3 NA

132 285 246 246 249 224 132 280 241 248 262 281 281 248 248 108 108 175 175 74 220 294 256 306 306 163 163 248 248 283 241 213

CAG AAG del1c ATA AGC AAG GAG ACA TGC CAG GTT CAC TAC CAG CAG del3b del3a CAC CAC del6a TGT TAG ins2 TGA TGA TGC TGC TGG TGG TGC del1b TGA

Lys Glu Met Met Arg Glu Lys Arg Ser Arg Gly Asp Asp Arg Arg Gly Gly Arg Arg Ala Tyr Glu Thr Arg Arg Tyr Tyr Arg Arg Arg Ser Arg

Gln Lys Fs. Ile Ser Lys Glu Thr Cys Gln Val His Tyr Gln Gln InF InF His His InF Cys Stop Fs. Stop Stop Cys Cys Trp Trp Cys Fs. Stop

BT-20 BT-474 BT-483 BT-483 BT-549 CAL-148 CAL-85-1 CAMA-1 EVSA-T H-31 H-71 HCC1007 HCC1008 HCC1143 HCC1143 HCC1187 HCC1187 HCC1395 HCC1395 HCC1419 HCC1419 HCC1569 HCC1806 HCC1937 HCC1937 HCC1954 HCC1954 HCC2157 HCC2157 HCC2218 HCC2713 HCC2998

14 165 1 33 34 8 25 92 36 883 14 41 16 883 883 1 1 1187 1187 1 336 54 2 160 160 140 140 728 728 27 26 306

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

24 24 2029 2091 24 2249 2249 2029 2091 1689 1689 1396 2258 2249 2258 2258 2249 1396 2258 2249 2249 1396 1396 1396 2258 2258 2249 2249 2258 1396 2258 2249

10.86 0.58 NA 0.28 12.42 38.49 0.56 0.29 0 0 11.71 0.66 7.28 0 0 NA NA 12.41 12.41 NA 1.21 NA NA NA NA 18.3 18.3 0 0 25.27 NA NA

AAG GAG ATG ATG AGG GAG AAG AGA TCC CGG GGT GAC GAC CGG CGG GGT GGT CGC CGC GCC TAT GAG ACA CGA CGA TAC TAC CGG CGG CGC TCC CGA

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM


Single report Single report Confirmed in another publication. wt in COSMIC Confirmed in three other publications Controversy with other publications Controversy with other publications Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC wt in COSMIC Single report Single report Single report Mutation in COSMIC database

Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Confirmed in another publication

Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database v 1.0

65

Cos. WT

Mut

A

Mut

Comp Name

NB

Cancer

Ref

273 273 248 213 249 157 157 215 244 285 261 280 220 166 236 266 204 273 368 273 132 234 244 244 277 136 273

CGT CGT CGG CGA AGG GTC GTC AGT GGC GAG AGT AGA TAT TCA TAC GGA GAG CGT CAC CGT AAG TAC GGC GGC TGT CAA CGT

CTT CTT CAG TGA GGG TTC TTC ATT AGC AAG del26a AAA TGT TAA TGC GAA ins7c CAT del30 CAT AGG TAA AGC AGC TTT TAA TGT

Arg Arg Arg Arg Arg Val Val Ser Gly Glu Ser Arg Tyr Ser Tyr Gly Glu Arg His Arg Lys Tyr Gly Gly Cys Gln Arg

Leu Leu Gln Stop Gly Phe Phe Ile Ser Lys Fs. Lys Cys Stop Cys Glu Fs. His InF His Arg Stop Ser Ser Phe Stop Cys

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

HCC38 HCC38 HCC70 HDQ-P1 HMT-3909 Hs 578T HS578T L56BR-X1 MAST MDA-MB-134-VI MDA-MB-157 MDA-MB-231 MDA-MB-330 MDA-MB-361 MDA-MB-415 MDA-MB-435 MDA-MB-436 MDA-MB-436 MDA-MB-453 MDA-MB-468 MFM-223 MW1C-6.3 OCUB-F OCUB-M R11T R18T R30T

147 147 883 306 52 177 177 25 72 165 1 78 336 19 75 74 1 780 1 780 51 10 72 72 48 47 687

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

1396 2258 1396 1713 552 76 2258 1968 1393 2029 2029 24 2091 2029 2091 1018 2029 1367 147 9 2249 147 2091 2249 1806 1806 1806

175 161 135 237 158 144 317

CGC GCC TGC ATG CGC CAG CAG

CAC GAC TTC ATA ins3a TAG TAG

Arg Ala Cys Met Arg Gln Gln

His Asp Phe Ile InF Stop Stop


SK-BR-3 SK-BR-5 SUM1315MO2 SUM149PT SUM159PT SUM185PE SUM190PT

1187 19 52 123 1 53 25

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

76 2091 2091 2091 2091 2091 2091


WAF1 Comments 0.86 0.86 0 NA 0.17 9.06 9.06 8.11 0.34 0.58 NA 0.46 1.21 NA 0.7 0 NA 1.01 NA 1.01 14.1 NA 0.34 0.34 0.31 NA 0.91

Single report Single report Single report Single report Confirmed in two other publications

Single report Single report wt in COSMIC wt in COSMIC Confirmed in another publication Single report wt in COSMIC Single report Single report Controversy with other publications Controversy with other publications wt in COSMIC Confirmed in another publication Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Confirmed in another publication. wt 12.41 in COSMIC 2.42 Single report 10.37 Single report 0.43 Single report NA Single report NA Single report NA Single report v 1.0

66

Cos. WT 265 273 213 194 342

CTG CGT CGA CTT CGA

Mut

A

CCG TGT TGA TTT TGA

Leu Arg Arg Leu Arg

Mut

Comp Name

Pro Cys Stop Phe Stop

SM SM SM SM SM

SUM225CWN SUM229PE SUM52PE T47D UACC-893

NB 23 687 306 28 74

Cancer

Ref

WAF1 Comments

Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma Breast carcinoma

2091 2091 2091 9 2029

0 0.91 NA 14.38 NA


Single report Single report Single report Confirmed in another publication Single report

v 1.0 67

Colorectal Tumors Table I : cell lines with wt p53 Cell line C32 C99 COLO678 Gp2D HCT116 LOVO LS180 LS174T LS513 NCI-747 RKO SKCO-1 Col15 TC7 EB

ATCC

CCL-247 CCL-229 CL-187 CL-188 CRL-2134 CRL-2577 HTB-39

Reference 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2051 2251 2251 2251

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) no data


68

v 1.0


Cancer

Ref

WAF1 Comments

282 CGG 248 CGG

TGG TGG

Arg Arg

Trp Trp

SM SM

RG VACO330

600 728

Colorectal adenoma Colorectal adenoma

1 1

0.55 0

Single report Single report

301 245 125 196 249 52 342 204

del1a AGC ATG TGA AGC TAA TGA TAG

Pro Gly Thr Arg Arg Gln Arg Glu

Fs. Ser Met Stop Ser Stop Stop Stop

SM SM SM SM SM SM SM SM

ala C10 C106 C125-PM C75 C80 C84 CACO2

6 440 12 241 34 6 74 46

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

724 2051 2051 2051 2051 2051 2051 2051

NA 0 14.64 NA 12.42 NA NA NA

234 TAC

TGC

Tyr

Cys

SM

CaR-1

133


1006

2.14

272 245 245 126 278 241 241 134 193 144 273 196 103 266 248 321 273 248 241 176 277 245

ATG AGC AGC TAG CAT TTC TTC TTG CGT TAG TGT TGA del27 GAA TGG ins2a CAT CAG TTC TTC TTT GAC

Val Gly Gly Tyr Pro Ser Ser Phe His Gln Arg Arg Tyr Gly Arg Lys Arg Arg Ser Cys Cys Gly

Met Ser Ser Stop His Phe Phe Leu Arg Stop Cys Stop InF Glu Trp Fs. His Gln Phe Phe Phe Asp

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

CaR-1 CBS CC07 CC20 CCK-81 CLONE A CLONE D Co74 Co82 Co84 Co92 CoCM-1 COLO-205 COLO-205 COLO-320 COLO-741 CX-1 CX27 DLD-1 FET FRI GLY

105 440 440 14 13 101 101 6 86 53 687 241 1 74 728 1 780 883 101 191 48 171

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

2051 724 2051 2051 2051 492 492 2258 2258 2258 2258 2051 492 1018 38 2051 492 1 27 724 724 724

8.79 0 0 NA 0.28 0 0 10.71 10.15 NA 0.91 NA NA 0 0 NA 1.01 0 0 22.88 0.31 1.95

Single report Single report Single report Single report Single report Single report Single report Single report Controversy with other publications. wt in COSMIC Controversy with other publications. wt in COSMIC Single report Single report Single report Single report Single report Single report

CCA GGC ACG CGA AGG CAA CGA GAG

GTG GGC GGC TAC CCT TCC TCC TTT CAT CAG CGT CGA TAC GGA CGG AAA CGT CGG TCC TGC TGT GGC


69

Single report Controversy with other publications Controversy with other publications Confirmed in two other publications Single report Single report Single report Confirmed in three other publications Single report Single report Single report v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 273 306 272 300 213 153 241 273 273 213 163 72 179 234 245 175 126 241 161 273 342 224 158 272 306 190 248 254 175 273 175 166 273 273 218

CGT CGA GTG CCC CGA CCC TCC CGT CGT CGA TAC CCC CAT TAC GGC CGC TAC TCC GCC CGT CGA GAG CGC GTG CGA CCT CGG ATC CGC CGT CGC TCA CGT CGT GTG

CAT TGA del2b del1a TGA GCC TTC CAT CAT CTA CAC del1b CGT CAC GAC CAC TAA TTC ACC CAT TGA GAT CTC ATG TGA CTT TGG ACC CAC CTT CAC TGA TGT CAT TTG


Arg Arg Val Pro Arg Pro Ser Arg Arg Arg Tyr Pro His Tyr Gly Arg Tyr Ser Ala Arg Arg Glu Arg Val Arg Pro Arg Ile Arg Arg Arg Ser Arg Arg Val

His Stop Fs. Fs. Stop Ala Phe His His Leu His Fs. Arg His Asp His Stop Phe Thr His Stop Asp Leu Met Stop Leu Trp Thr His Leu His Stop Cys His Leu

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD DMD

H-110 H-173 HCA46 HCA7 HCC-2998 HCT-15 HCT-15 HRA19 HT-29 HT55 ISRECO1 KM12 KM12 LIM1863 LS-1034 LS-123 LS-411 MIP 101 MOSER NCI-H508 NCI-H630 NCI-H716 NCI-H747 P-6 RCM-1 SNU-1033 SNU-1040 SNU-1047 SNU-1097 SNU-503 SNU-61 SNU-C1 SNU-C2B SNU-C2B SNU-C5

780 160 1 8 306 5 101 780 780 33 26 1 146 25 171 1187 11 101 75 780 74 6 92 105 160 52 728 11 1187 147 1187 18 687 780 3

Cancer

Ref

WAF1 Comments

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

1689 1689 2051 2051 1018 1018 2251 2051 492 2051 724 2249 1018 2051 724 2051 2051 492 492 2249 2249 2051 219 1006 2051 1204 1204 1204 1204 1204 1204 2249 2051 2051 1038

1.01 NA NA NA NA 65.41 0 1.01 1.01 0.69 11.22 NA 13.02 0 1.95 12.41 NA 0 13.25 1.01 NA 59.68 8.19 8.79 NA 10.12 0 0.85 12.41 0.86 12.41 NA 0.91 1.01 54.76

70

Single report Single report Single report Single report Confirmed in COSMIC database Controversy with other publications Controversy with other publications Single report Confirmed in two other publications wt in COSMIC Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report v 1.0


Cancer

Ref

WAF1 Comments

248 CGG

TGG

Arg

Trp

DMD

SNU-C5

728


1038

0

159 237 248 51

GCC ATG CGG GAA

GAC del14 CAG TAA

Ala Met Arg Glu

Asp Fs. Gln Stop

SM SM SM SM

SW1116 SW1417 SW1463 SW403

8 1 883 6

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

219 492 2249 2051

10.18 NA 0 NA

248 273 309 248 117 245 175 306 154 135 329 282 273 181 190 273

CGG CGT CCC CGG GGG GGC CGC CGA GGC TGC ACC CGG CGT CGC CCT CGT

TGG CAT TCC TGG del1a GAC CAC TGA AGC TGG ins1a TGG TGT TGC CTT CAT

Arg Arg Pro Arg Gly Gly Arg Arg Gly Cys Thr Arg Arg Arg Pro Arg

Trp His Ser Trp Fs. Asp His Stop Ser Trp Fs. Trp Cys Cys Leu His

SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

SW48 SW480 SW480 SW837 SW948 V9P VACO10MS VACO429 VACO457 VACO489 VACO4A VACO5 VACO576 VACO670 VACO8 WIDR

728 780 6 728 3 171 1187 160 14 25 1 600 687 28 52 780

Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma Colorectal carcinoma

492 9 9 9 2051 724 2051 2051 996 996 2051 996 996 996 996 27

0 1.01 43.57 0 NA 1.95 12.41 NA 11.47 12.8 NA 0.55 0.91 26.1 10.12 1.01


71

Single report Confirmed in two other publications. wt in COSMIC wt in COSMIC Mutation in COSMIC database Single report Confirmed in another publication. wt in COSMIC Single report Single report Confirmed in two other publications wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication

v 1.0

Gastric Tumors Table I : cell lines with wt p53 Cell line SNU-520 SNU-719 NUGC-4 STKM-2 MKN-45 MKN-74

ATCC

-

Reference 747 747 462 1006 462 462

Table II : cell lines with p53 gene deletion or rearrangement Cell line KATO III

ATCC HTB-103

Reference

Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) No data


72

v 1.0


CGG CGG GCA CGC GAG CAT CAG CTG CGT CCT GTG CCG GGC CAT GTG GTG GTG ATC CCT

Cancer

Ref

WAF1 Comments

728 600 1 1187 10 14 18 10 687 2 105 2 440 18 77 20 20 5 87

Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma

71 1393 2249 1393 2249 2249 2249 1689 1689 1689 1689 2249 2087 2242 94 94 71 94 2087

0 0.55 NA 12.41 67.48 17.51 NA 12.61 0.91 NA 8.79 NA 0 9.99 10.53 7.49 11.35 0 0.34

TGG TGG del7b CAC CAG CAG TAG CGG TGT del37 ATG ins1c AGC CCT ATG GCG GCG CTC TCT

Arg Arg Ala Arg Glu His Gln Leu Arg Pro Val Pro Gly His Val Val Val Ile Pro

Trp Trp Fs. His Gln Gln Stop Arg Cys Fs. Met Fs. Ser Pro Met Ala Ala Leu Ser

SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

20M AKG ECC4 G42LATE GCIY GCIY GT3TKB H-111 H-162 H-30 H-55 HGC-27 HSC-39 HUG-1N JR1 KWS MKN-1 MKN-28 MKN-7

251 ATC

CTC

Ile

Leu

DMU

MKN-74

5

Gastric carcinoma

1006

251 ATC

CTC

Ile

Leu

SM

MKN-74

5

Gastric carcinoma

94

271 248 220 342 57 175 281 205 216 266 143 273

GCG CAG TGT TGA del17 CAC GAG TTT ATG GAA del1 CAT

Glu Arg Tyr Arg Asp Arg Asp Tyr Val Gly Val Arg

Ala Gln Cys Stop Fs. His Glu Phe Met Glu Fs. His

DMU SM SM SM SM SM SM SM SM SM SM SM

MKN-74 NCI-N87 NUGC-3 OKAJIMA SK-GT-1 SK-GT-2 SK-GT-5 SNU-16 SNU-216 SNU-484 SNU-55 SNU-601

3 883 336 74 2 1187 26 3 74 74 2 780

Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma

1006 46 94 71 462 462 462 46 747 747 46 747

GAG CGG TAT CGA GAC CGC GAC TAT GTG GGA GTG CGT


73

Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Confirmed in another publication Single report Single report Controversy with other publications. 0 wt in COSMIC Controversy with other publications. 0 wt in COSMIC Controversy with other publications. 16.97 wt in COSMIC 0 Single report 1.21 Confirmed in another publication NA Single report NA Single report 12.41 Single report 1.66 Single report 8.34 Single report 0.16 Single report 0 Single report NA Single report 1.01 Single report v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 282 215 273 381 173 175

CGG AGT CGT AAA GTG CGC

TGG AAT TGT del1a ATG CAC


Arg Ser Arg Lys Val Arg

Trp Asn Cys Fs. Met His

SM SM DMU DMU SM SM

SNU-638 SNU-668 TGBC11TKB TGBC11TKB TMK-1 YCC-3

600 15 687 1 77 1187

Cancer

Ref

WAF1 Comments

Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma Gastric carcinoma

747 747 2249 2249 71 46

0.55 3.97 0.91 NA 10.53 12.41

74

Single report Single report Mutation in COSMIC database Mutation in COSMIC database Confirmed in another publication Single report

v 1.0

Cervical carcinoma Table I : cell lines with wt p53 Cell line HeLa* ME-180* SKG-IIIa* SW756* CA-SKI* SIHA* KB* MS-715* C4II* SKG-II*

ATCC CCL-2 HTB-33 CRL-10302 CRL-1550 HTB-35 CCL-17 HTB-34 CRL-1595

Reference 1006 1006 1006 2249 2249 2249 2249 68 68

* HPV positive

Table II : cell lines with p53 gene deletion or rearrangement no data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) no data Ovarian Tumors Table I : cell lines with wt p53 Cell line KGN A2780 RMG-1 CH1 LK1 LK2

ATCC

Reference 2249 2249 2249 925 925 925

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) No data


75

v 1.0


Cancer

Ref

WAF1 Comments

109 273 245 175 131

TTC CGT GGC CGC AAC

TTA TGT GTC CAC del3a

Phe Arg Gly Arg Asn

Leu Cys Val His InF

SM SM SM SM SM

C2 C33A HT-3 IGR/Cut40 SKS

1 687 84 1187 7

Cervical Cancer Cervical Cancer Cervical Cancer Cervical Cancer Cervical Cancer

469 54 54 573 1782

9.61 0.91 0 12.41 NA

213 89 389 273 213 248 286 195 248 248 154 273 138 282 246 246

CGA CCC GGG CGT CGA CGG GAA ATC CGG CGG GGC CGT GCC CGG ATG ATG

CAA del1c TGG ins1a TGA CAG AAA AAC CAG CAG GAC CAT GTC TGG GTG GTG

Arg Pro Gly Arg Arg Arg Glu Ile Arg Arg Gly Arg Ala Arg Met Met

Gln Fs. Trp Fs. Stop Gln Lys Asn Gln Gln Asp His Val Trp Val Val

SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

AN3 AN3-CA AN3-CA EN ESS-1 HEC-116 HEC-155 HEC-180 HEC-1-A HEC-1-B HEC-251 HEC-59 HHUA HOUA IK-90 ISHIKAWA

38 1 1 2 306 883 86 14 883 883 9 780 48 600 57 57

Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor

64 2249 2249 1585 2249 1935 1935 1935 64 64 1935 1935 339 339 339 64

2.19 NA 47.89 NA NA 0 11.07 10.54 0 0 39.65 1.01 31.84 0.55 0 0

175 306 156 218 218

CGC CGA CGC GTG GTG

CAC TGA del1a del3 del3a

Arg Arg Arg Val Val

His Stop Fs. InF InF

SM SM DMU SM DMU

KLE MFE-296 RL95-2 RL95-2 RL95-2

1187 160 3 2 3

Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor Endometrial tumor

64 2249 1625 64 1625

Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publications. wt 12.41 in COSMIC NA Mutation in COSMIC database NA Controversy with other publications NA Controversy with other publications NA Controversy with other publications

175 CGC

CAC

Arg

His

SM

FT-MZ-1

1187

Fallopian tube ca.

590

12.41 Single report

99 TCC 316 CCC 273 CGT

del2b CCT CAT

Ser Pro Arg

Fs. Pro His

SM SM SM

6 222 2774

1 6 780

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

376 798 798

NA NR 1.01


Single report Confirmed in two other publications Confirmed in another publication Single report Single report

Single report Single report Single report v 1.0

76

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 172 193 172 196 193 136 147 213 124 273 143 195 194 306 282 126 270 282 281 126 273 215 126 277 248 130 224 126

GTT CAT GTT CGA CAT CAA GTT CGA TGC CGT GTG ATC CTT CGA CGG TAC TTT CGG GAC TAC CGT AGT TAC TGT CGG CTC GAG TAC

Cancer

Ref

WAF1 Comments 8.17 11.02 8.17 NA 10.15 NA 11.64 NA 8.62 0.91 NA 11.24 10.61 NA 0.55 11.62 8.14 0.55 7.28 NA 1.01 1.17 11.62 0.31 0 8.89 NA NA

TTT CTT TTT TGA CGT TAA GAT TGA CGC TGT del32 ACC CGT TGA TGG TGC TTA TGG TAC del21a CAT CGT TGC TTT CAG GTC ins3 del18

Val His Val Arg His Gln Val Arg Cys Arg Val Ile Leu Arg Arg Tyr Phe Arg Asp Tyr Arg Ser Tyr Cys Arg Leu Glu Tyr

Phe Leu Phe Stop Arg Stop Asp Stop Arg Cys Fs. Thr Arg Stop Trp Cys Leu Trp Tyr InF His Arg Cys Phe Gln Val InF InF

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM SM SM SM SM SM SM SM SM SM

2780CP A. P. A2780-CP-20 C. P./1 C. V. Caov-3 Caov-4 DDL11 EFO-21 EFO-27 EG F. P. G. C. G. M. GBM IGROV-1 IGROV-1/Pt IGROV-1/Pt KURAMOCHI NCI/ADR-RES OC-314 OV 90 OV1P OVCA 432 OVCAR-3 OVCAR-4 OVCAR-5 OVCAR-8

19 55 19 241 86 47 7 306 4 687 1 90 66 160 600 17 7 600 16 6 780 4 17 48 883 22 1 1

Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma Ovarian carcinoma

963 1011 798 1011 1011 144 144 1011 2249 2249 798 1011 1011 1011 1011 2249 606 606 144 983 2249 1165 925 1011 144 2249 864 864

GAC

Asn

Asp

SM

PA-1

53

Ovarian carcinoma

854

316 CCC CCT Pro 195 ATC ACC Ile 275 TGT TAT Cys 273 CGT CAT Arg 89 CCC del1a Pro Cell line p53 Handbook

Pro Thr Tyr His Fs.

SM SM SM SM SM

PA-1 PM1015 R. B. S. P. SK-OV-3

6 90 87 780 3


144 798 1011 1011 2249

239 AAC

77

Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report wt in two other publications Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Single report wt in another publication wt in COSMIC Single report Controversy with other publications. 20.19 wt in COSMIC Controversy with other publications. NR wt in COSMIC 11.24 Single report 0.42 Single report 1.01 Single report NA Controversy with other publications v 1.0


Cancer

Ref

WAF1 Comments

179 262 273 175 175

CAT GGT CGT CGC CGC

CGT GTT CAT CAC CAC

His Gly Arg Arg Arg

Arg Val His His His

SM SM SM SM SM

SK-OV-3 SW626 SW626 TOV 112D TYK-nu

146 14 780 1187 1187


1018 864 1011 1165 2249

13.02 11.71 1.01 12.41 12.41

Controversy with other publications Controversy with other publications Controversy with other publications Single report Mutation in COSMIC database

273 151 266 248 171 273 314 151 155 245 249 248

CGT CCC GGA CGG GAG CGT TCC CCC ACC GGC AGG CGG

CAT CAC GTA CAG TAG TGT TCT CAC ATC AGC del1b CAG

Arg Pro Gly Arg Glu Arg Ser Pro Thr Gly Arg Arg

His His Val Gln Stop Cys Ser His Ile Ser Fs. Gln

SM SM SM SM SM DMU DMU SM SM DMU DMU SM

A-431 CAL-39 SW962 UM-SCA-1 UM-SCV-2 UM-SCV-3 UM-SCV-3 UM-SCV-4 UM-SCV-5 UM-SCV-7 UM-SCV-7 UT-SCV-1

780 33 51 883 25 687 2 33 23 440 26 883

Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC Vulvar SCC

153 2249 2249 1625 681 681 681 681 681 681 681 1625

1.01 10.75 0 0 NA 0.91 NR 10.75 8.85 0 NA 0

Confirmed in two other publications Mutation in COSMIC database Mutation in COSMIC database Single report Confirmed in another publication Confirmed in another publication Confirmed in another publication Confirmed in another publication Single report Confirmed in another publication Confirmed in another publication Single report


v 1.0 78

Hepatocellular carcinoma Table I : cell lines with wt p53 Cell line HuH-6 clone 5 HuH-1* HCC-M* SK-Hep1 HepG2 WRL 68 FOCUS*,**

ATCC

HTB-52 HB-8065

Reference 1006 1042 1042 1042 1042 230 232

HBV positive Table II : cell lines with p53 gene deletion or rearrangement Cell line Hep3B*

ATCC HB-8064

Reference



79

v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 281 139 249 244 249 272 244 264 220 249 249 164 161 262 200

GAC AAG AGG GGC AGG GTG GGC CTA TAT AGG AGG AAG GCC GGT AAT

CAC GAG AGT GCC AGC ATG GCC del18 TGT AGT AGT TAG ACC GAT AAA


Asp Lys Arg Gly Arg Val Gly Leu Tyr Arg Arg Lys Ala Gly Asn

His Glu Ser Ala Ser Met Ala InF Cys Ser Ser Stop Thr Asp Lys

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

OHR HCC-T HCKI-1 HLE HLE HLE HLF HuH-4 HuH-7 Malhavu PLC/PRF/5 SNU-387 SNU-449 SNU-475 TONG/HCC

41 3 389 10 34 105 10 1 336 389 389 18 75 6 1

Cancer

Ref

WAF1 Comments

hepatoblastoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma

589 1042 1356 1069 230 1006 230 230 230 232 38 2249 2249 2249 1042

0.66 60.6 12.42 0 12.42 8.79 0 NA 1.21 12.42 12.42 NA 13.25 11.22 22.72

80

Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Confirmed in two other publications Single report wt in another publication Single report Confirmed in two other publications Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in another publication

v 1.0

Head and Neck SCC Table I : cell lines with wt p53 Cell line MO24 PCI-30 UM-SCC-17A/B UM-SCC-25 UM-SCC-47 UM-SCC-72 UM-SCC-74A UM-SCC-74B UM-SCC-81A UPCI-SCC-3 UPCI-SCC-30 UPCI-SCC-40 UPCI-SCC-56 UPCI-SCC-81 UPCI-SCC-104 UPCI-SCC-131 UPCI-SCC-142

ATCC

UPCI-SCC-154 HOC927 BICR-10 BICR-18 MSK-922 MSK-121 MDA-1986 MDA-886 584 185 1483 183A NHOK HOK-16B HOK-18A HAp-2

Ref. 2252 2252 2252 2252 2252 2252 2252 2252 2252 1544 1544 1544 1544 1544 1544 1544 1544

1544 105 208 208 600 600 600 600 600 600 600 600 626 626 626 626

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV)

Cell line HSC-2 BICR-22 NU


ATCC

Mutation Splice intron 6 Splice intron 8 Splice intron 6

81


v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 175 281 258 180 275 183 146 332 308 282 173 126 192 151 248 193 175 188 213 248 248 267 193 266 285 126 281 205 305 248 237 266 194 298

CGC GAC GAA GAG TGT TCA TGG ATC CTG CGG GTG TAC CAG CCC CGG CAT CGC CTG CGA CGG CGG CGG CAT GGA GAG TAC GAC TAT AAG CGG ATG GGA CTT GAG

CAC CAC AAA del1b TGG TAA TGA del99 del19 CCG del3b del21 TAG CAC TGG CTT CAC del12 TGA CTG TGG TGG CTT CGA AAG TAG GAG TGT ins1a CAG ATA GAA CGT TAG

Arg Asp Glu Glu Cys Ser Trp Ile Leu Arg Val Tyr Gln Pro Arg His Arg Leu Arg Arg Arg Arg His Gly Glu Tyr Asp Tyr Lys Arg Met Gly Leu Glu

His His Lys Fs. Trp Stop Stop InF Fs. Pro InF InF Stop His Trp Leu His InF Stop Leu Trp Trp Leu Arg Lys Stop Glu Cys Fs. Gln Ile Glu Arg Stop

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

Detroit562 TYS 4197 A253 A30 BB30-HNC BICR-16 BICR-19 BICR-22 BICR-3 BICR-31 BICR-56 BICR-6 BICR-7 Ca9-22 CAL-27 CAL-33 DOK EVSCC1 FaDu FS-1 GL HN HO-1-N-1 HOC313 HOC605 HOC719 HOC815 HSC-3 HSC-4 HSC-5 HSQ-89 HU 281 JSQ-3

1187 41 73 1 11 3 51 1 1 21 2 4 85 33 728 55 1187 1 306 124 728 37 55 20 165 14 26 117 1 883 123 74 66 71

Cancer

Ref

WAF1 Comments

Head and Neck Head and Neck Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC

2249 1056 1076 2249 2032 2249 208 208 208 208 208 208 208 208 105 1854 2249 418 153 153 2252 1846 2249 2249 105 105 105 105 105 105 105 2252 202 160

12.41 0.66 0.31 NA 0.73 NA NA NA NA 0 NA NA NA 10.75 0 11.02 12.41 NA NA 0 0 1.68 11.02 10.77 0.58 NA 1.66 8.94 NA 0 0.43 0 10.61 NA


Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Confirmed in two other publications Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

82


CAA CGG TGC CAT GAA AGA CGG TAC CGT GTG ACA CCC CCC CGC GGC CGA TAT CGG AAG GTG GGA CGC CTG GAG AGA TCT TGC AGG ACA CCT TCT TAC TTC CAT

ins3a TGG TGG CGT GGA CCA TGG TGC CTT GGG del1c TCC TCC CCC AGC TGA CAT TGG GAG TTG GAA CGG CTA AAG ACA TCC TTC AGA GCA CCC CCT TAG TAC TAT

Gln Arg Cys His Glu Arg Arg Tyr Arg Val Thr Pro Pro Arg Gly Arg Tyr Arg Lys Val Gly Arg Leu Glu Arg Ser Cys Arg Thr Pro Ser Tyr Phe His

InF Trp Trp Arg Gly Pro Trp Cys Leu Gly Fs. Ser Ser Pro Ser Stop His Trp Glu Leu Glu Arg Leu Lys Thr Ser Phe Arg Ala Pro Pro Stop Tyr Tyr

SM SM SM SM SM DMU DMU SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM MM MM MM SM DMU DMU SM DMU DMU DMU DMU SM DMU

KOSC-2 KOSC-3 KUMA-3 LB771-HNC MDA-1186 MDA-1386 MDA-1386 MDA-1483 MDA-1586 MDA-1686 MDA-1686 MDA-183 MDA-686 MKG 7 MSK-QLL1 MSK-QLL2 NA NOS-1 OC2 OC3 OM1 OSC-1 OSC-1 OSC-1 OSC-2 OSC-3 OSC-3 OSC-4 OSC-5 OSC-5 OSC-6 OSC-6 OSC-7 OSC-8

1 728 19 146 20 1 600 17 147 11 1 92 92 35 72 241 15 728 25 68 74 5 2 1 92 2 191 7 3 2 1 14 2 41

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

2249 598 2252 2249 600 600 600 2032 600 600 600 2032 600 1846 600 600 105 1784 1854 2224 105 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207 1207

NA 0 15.16 13.02 0 NR 0.55 11.62 0.86 61.46 NA 0.85 0.85 0.18 0.34 NA 0.97 0 0.56 3.61 0 NR NR 60.67 0.29 NR 22.88 NR 52.88 NR 63.47 NA 60.64 4.97


Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Single report wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

83


ACA CGT CCC GAA GAG GTG GAA GAG AGA CGC CGT CCC GAA GTT AGG CGA GAG CCC GTT CGG GAG CCC GAG CCG CAT TAT CGG ACC GAA TTT TAT GGC GGC TGC

ATA CAT ACC AAA TAG GGG AAA ins5c del2a CAC CAT TCC AAA del32c AGC TGA del1 TCC del32 TGG del46c TCC TAG del13 TAT TGT CTG AAC TAA del1a CAT TGC TGC TCC

Thr Arg Pro Glu Glu Val Glu Glu Arg Arg Arg Pro Glu Val Arg Arg Glu Pro Val Arg Glu Pro Glu Pro His Tyr Arg Thr Glu Phe Tyr Gly Gly Cys

Ile His Thr Lys Stop Gly Lys Fs. Fs. His His Ser Lys Fs. Ser Stop Fs. Ser Fs. Trp Fs. Ser Stop Fs. Tyr Cys Leu Asn Stop Fs. His Cys Cys Ser

DMU DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

OSC-8 OSC-9 OSC-9 PCI-13 SAS SCC-12B SCC-13 SCC-15 SCC-25 SCC-27 SCC-35 SCC-4 SCC-4451 SCC-9 SKX SQ-38 SQ9G SSC-4 SSC-9 TSU TU-139 TU-177 UD-SCC-3 UD-SCC-4 UD-SCC-5 UD-SCC-6 UD-SCC-7 UD-SCC-8 UMB-SCC-745 UMB-SCC-864 UMB-SCC-969 UM-SCC-10A UM-SCC-10B UM-SCC-11B

6 780 2 86 5 5 73 1 20 1187 780 92 73 1 34 241 2 92 1 728 1 92 10 1 128 336 124 34 7 3 12 86 86 19

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

1207 1207 1207 2252 2249 208 208 626 208 208 160 208 1937 2249 1846 160 160 202 160 105 626 626 1636 1636 1636 1636 1636 1636 2245 2245 2245 153 153 1636

5.83 1.01 60.26 11.07 NA 2.05 0.31 NA NA 12.41 1.01 0.85 0.31 NA 12.42 NA NA 0.85 NA 0 NA 0.85 NA NA 13.27 1.21 0 8.59 NA NA 6.44 0 0 0


Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Confirmed in COSMIC database Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

84


CAG TAC TGT TGT AGA CGG GAT CCC TAT TGC CGT CGG CGC CGA CCT GTC CGT TGC CGG CAT CGA ACC CAT CGG CGC CGT CCC GAG CGG ACC GAG AGA ATC CGG

TAG TGC del30 del30 AGT CTG del10 del2a TGT TTC TGT CAG CCC TGA GCT TTC CTT TTC TGG CGT TGA CCC TAT CAG CAC CAT CAC TAG TGG CCC TAG ACA TTC CAG

Gln Tyr Cys Cys Arg Arg Asp Pro Tyr Cys Arg Arg Arg Arg Pro Val Arg Cys Arg His Arg Thr His Arg Arg Arg Pro Glu Arg Thr Glu Arg Ile Arg

Stop Cys InF InF Ser Leu Fs. Fs. Cys Phe Cys Gln Pro Stop Ala Phe Leu Phe Trp Arg Stop Pro Tyr Gln His His His Stop Trp Pro Stop Thr Phe Gln

SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

UM-SCC-12 UM-SCC-13 UM-SCC-14 UM-SCC-14 UM-SCC-14 UM-SCC-16 UM-SCC-19 UM-SCC-20 UM-SCC-22 UM-SCC-23 UM-SCC-27 UM-SCC-3 UM-SCC-36 UM-SCC-4 UM-SCC-46 UM-SCC-5 UM-SCC-57 UM-SCC-63 UM-SCC-68 UM-SCC-81B UPCI:SCC-103 UPCI:SCC-105 UPCI:SCC-111 UPCI:SCC-114 UPCI:SCC-116 UPCI:SCC-122 UPCI:SCC-125 UPCI:SCC-136 UPCI:SCC-16 UPCI:SCC-172 UPCI:SCC-182 UPCI:SCC-29B UPCI:SCC-36 UPCI:SCC-70

18 140 2 2 14 124 2 1 336 191 687 883 21 306 24 177 147 88 728 86 160 20 128 883 1187 780 33 10 600 20 54 92 29 883

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC

1983 1983 1019 1636 1636 153 153 1983 2245 153 2245 2245 1983 2245 1983 2248 1983 153 1983 1983 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544 1544

NA 18.3 NA NA 20.55 0 NA NA 1.21 22.88 0.91 0 10.17 NA 14.87 9.06 0.86 13.79 0 10.15 NA 7.8 13.27 0 12.41 1.01 10.75 NA 0.55 7.8 NA 0.29 10.47 0


Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report v 1.0

85

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 179 177 196 144 306 342 110 232 285 275 248 238 282 282 248 151 266 255 273 282 236 258 273 279

CAT CCC CGA CAG CGA CGA CGT ATC GAG TGT CGG TGT CGG CGG CGG CCC GGA ATC CGT CGG TAC GAA CGT GGG

AAT CGC TGA TAG TGA TGA TGT AAC AAG TTT TGG TTT CCG TGG del9 CAT GAA TTC CTT TGG TAA GCA CAT GAG

His Pro Arg Gln Arg Arg Arg Ile Glu Cys Arg Cys Arg Arg Arg Pro Gly Ile Arg Arg Tyr Glu Arg Gly

Asn Arg Stop Stop Stop Stop Cys Asn Lys Phe Trp Phe Pro Trp InF His Glu Phe Leu Trp Stop Ala His Glu

SM SM SM DMU DMU SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

UPCI:SCC-72 UPCI:SCC-99 UT-SCC-1 UT-SCC-10 UT-SCC-10 UT-SCC-12 UT-SCC-16 UT-SCC-16 UT-SCC-19 UT-SCC-2 UT-SCC-20 UT-SCC-22 UT-SCC-30 UT-SCC-33 UT-SCC-4 UT-SCC-5 UT-SCC-7 UT-SCC-8 VU1131 VU1365 YD-10B YD-15 YD-8 ZA

23 19 241 53 160 74 11 13 165 47 728 42 21 600 1 3 74 37 147 600 12 3 780 42

Cancer

Ref

WAF1 Comments

Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and Neck SCC Head and neck SCC

1544 1544 1019 1019 1019 2250 1019 1019 2250 1019 1019 1636 1019 1636 2250 1019 2250 2250 1996 1996 2189 2189 2189 105

19.3 17.88 NA NA NA NA 10.91 0.72 0.58 0.02 0 0.82 0 0.55 NA 10.75 0 0.48 0.86 0.55 NA 0 1.01 0.27


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report

v 1.0 86

Leukemia / Lymphoma Table I : cell lines with wt p53 Cell line

ATCC

Origin AML

1006

CCL-159

CML-B

1006

J-111 IM-9

Ref

WMN FWL

Burkitt lymphoma Lymphoblastoid

447 447

NL2

Lymphoblastoid

447

AG876

Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma ALL

447

1627

ML-1

CML

KMS-12BM KMS-12-PE

Myeloma

1006

Myeloma

1006

Myeloma

1006 613

JJN-3

Diffuse large cell lymphoma Diffuse large cell lymphoma Myeloma

98

UoC-B1

ALL

1627

XG-3

Myeloma

98

UoC-B3

ALL

1627

PL-21

126

UoC-B4

ALL

1627

ALL-B

Myeloid Leukemia ALL

1303

UoC-B11

ALL

1627

ALL-G

ALL

1303

SUP-B7

ALL

1627

ALL-K

ALL

1303

SUP-B13

ALL

1627

NGR

ALL

1303

SUP-B15

ALL

1627

SCO-1

ALL

1303

EU-1

ALL

1627

BRE

ALL

1303

EU-3

ALL

1627

HAU

ALL

1303

RPMI-1788

CCL156

Ly3 DHL4

SHO JLP119 EW36

613

Reh

CRL-8286

447 447 447

Table II : cell lines with p53 gene deletion or rearrangement Cell line HL-60 EU-4

ATCC CCL-240

Origin promyelocytic leukemia ALL



v 1.0 87


Cancer

306 236 237 248 133 248 248 248 272 241 273 241 172 238 195 288

CGA TAC ATG CGG ATG CGG CGG CGG GTG TCC CGT TCC GTT TGT ATC AAT

TGA CAC GTG CAG AAG TGG TGG CAG ATG CCC CAT del1c TTT TAT ACC TAT

Arg Tyr Met Arg Met Arg Arg Arg Val Ser Arg Ser Val Cys Ile Asn

Stop His Val Gln Lys Trp Trp Gln Met Pro His Fs. Phe Tyr Thr Tyr

SM MM MM MM SM SM SM SM SM SM SM SM DMU DMU SM SM

2L1 CG2 CG2 CG2 HEL K051 K052 KASUMI-1 KMOE-2 ML-1 MONOMAC-1 NOMO-1 OHN-GM OHN-GM SHI-1 YSK-21

160 13 14 883 13 728 728 883 105 11 780 4 19 98 90 5

Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous Acute Myelogenous

109 265 248 109 248 248 248 273 248 273 248 246 124 273 224 225 177 245

TTC CTG CGG TTC CGG CGG CGG CGT CGG CGT CGG ATG TGC CGT GAG GTT CCC GGC

TCC CCG CAG TCC CAG CAG CAG CTT CAG CTT CAG ACG TGA TGT ins5 ATT TCC AGC

Phe Leu Arg Phe Arg Arg Arg Arg Arg Arg Arg Met Cys Arg Glu Val Pro Gly

Ser Pro Gln Ser Gln Gln Gln Leu Gln Leu Gln Thr Stop Cys Fs. Ile Ser Ser

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

ALL-A ALL-C ALL-PO ALL-W EU-10 EU-11 EU-13 EU-18 EU-2 EU-6 EU-7 GR-ST HPB-ALL KARPAS-45 KG-1 KG-1a KMO-90 KOPM30

3 23 883 3 883 883 883 147 883 147 883 13 1 687 1 4 19 440

B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic


88

Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia

Ref

WAF1 Comments

2048 356 356 356 1006 243 243 2249 2249 2198 2198 2249 2049 2049 2207 1710

NA 14.28 13.83 0 10.08 0 0 0 8.79 0.48 1.01 NA 8.17 14.58 11.24 50.99

from patient RUPN84. Single report Single report Single report Single report wt in COSMIC Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Mutation in COSMIC database Single report Single report Single report Single report

1303 1303 2249 1303 1627 1627 1627 1627 617 617 617 2249 2198 2249 126 163 239 661

11.17 0 0 11.17 0 0 0 0.86 0 0.86 0 0 NA 0.91 NA 57.07 34.33 0

Single report Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 245 257 282 209 175 248 358 246 175 220 281 248 132 285 237 330 280 127 176 248 273 274 213 234 283 216 273 158 176 238 176 245 282

Cancer

GGC CTG CGG AGA CGC CGG GAG ATG CGC TAT GAC CGG AAG GAG ATG CTT AGA TCC TGC CGG CGT GTT CGA TAC CGC GTG CGT CGC TGC TGT TGC GGC CGG

CGC GTG ins2b ins4b TGC CAG AAG ACG CAC TGT GGC CTG AGG AAG ATA CAT CGA TTC TCC CAG TGT CTT CAA CAC CAC ATG CAT CAC GGC CGT TTC GAC CCG

Gly Leu Arg Arg Arg Arg Glu Met Arg Tyr Asp Arg Lys Glu Met Leu Arg Ser Cys Arg Arg Val Arg Tyr Arg Val Arg Arg Cys Cys Cys Gly Arg

Arg Val Fs. Fs. Cys Gln Lys Thr His Cys Gly Leu Arg Lys Ile His Arg Phe Ser Gln Cys Leu Gln His His Met His His Gly Arg Phe Asp Pro

SM SM SM SM MM MM MM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMD DMD SM DMU DMU DMU DMU SM SM SM SM

KOPN32 KOPN35 KOPN49 KOPN63 SCMC-L9 SCMC-L9 SCMC-L9 Tanoue TMBL-1 U-698-M BALL-1 HL-60(TB) KU812 RPMI-8226 WI-L2-NS WI-L2-NS 2 3 5 6 8 9 A3/KAW A3/KAW DG-75 HT HT LY1 LY1 LY17 LY2 LY7 LY8 C3

20 7 1 1 28 883 1 13 1187 336 16 124 51 165 123 4 1 30 13 883 687 16 38 25 19 74 780 105 7 26 191 171 21

B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-Acute Lymphoblastic B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Leukemia B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma B-cell Lymphoma

248 CGG

CAG

Arg

Gln

SM

DB

883

B-Lineage Diffuse Large Cell L


89

Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia Leukemia

Ref

WAF1 Comments

661 661 661 661 478 478 478 2242 1967 2249 2242 1018 1006 1018 637 769 1098 1098 1098 1098 1098 1098 3 3 1143 2249 2249 613 613 613 613 613 613

7.9 1.08 NA NA 61.6 0 54.45 0 12.41 1.21 12.06 0 14.1 0.58 0.43 9.21 NR 12.47 13.27 0 0.91 1.22 2.19 0 0.46 0.16 1.01 8.78 15.95 0.48 22.88 1.95 0

2249

0

Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report wt in COSMIC Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Mutation in COSMIC database v 1.0


CGT AAG CGT CCT CGG TTG CAT CGT ATG ATG CGG TGC CGG CGG CGT CGA CGG CGC CGA GGA CGG TTG CCG ATC TAC AAG CGG CGG TGT TGT CGG TAC GAG CGT CGA

TGT TAG TGT del1 CAG del1 CGT TGT ACG ATA CAG TAC TGG TGG TGT TGA CAG CAC TGA GAA CAG del1 CTG AAC TGC CAG CAG CAG TAT TAT CAG CAC TAG TGT CAA


Arg Lys Arg Pro Arg Leu His Arg Met Met Arg Cys Arg Arg Arg Arg Arg Arg Arg Gly Arg Leu Pro Ile Tyr Lys Arg Arg Cys Cys Arg Tyr Glu Arg Arg

Cys Stop Cys Fs. Gln Fs. Arg Cys Thr Ile Gln Tyr Trp Trp Cys Stop Gln His Stop Glu Gln Fs. Leu Asn Cys Gln Gln Gln Tyr Tyr Gln His Stop Cys Gln

SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM DMD DMD SM SM SM SM SM SM SM DMU DMU SM DMD

KARPAS-299 KARPAS-422 Abana AKATA AKUA AS283A BJAB BL-113 BL-30 BL-37 BL-41 BL-49 BL-49 BL-60 BL-70 BL-99 CA46 CW678 DAUDI DAUDI DH978 EB3 GA-10 GA-10 JD38 JIYOYE KK124 KK125 LOUCKES MC116 NAMALWA P3HR1 P3HR1 PP984 RAJI

687 5 687 1 883 2 86 687 13 123 883 88 728 600 687 306 883 1187 306 74 883 2 91 13 133 14 883 883 98 98 883 26 14 687 38

Cancer

Ref

WAF1 Comments

B-Lineage Diffuse Large Cell L B-Lineage Diffuse Large Cell L Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma

2249 2249 2247 75 447 44 75 44 75 44 75 44 44 44 2249 44 447 44 44 2249 1030 44 1819 1819 44 75 1007 44 75 44 75 44 44 44 161

0.91 NA 0.91 NA 0 NA 10.15 0.91 0 0.43 0 14.82 0 0.55 0.91 NA 0 12.41 NA 0 0 NA 9.52 0.72 2.14 10.86 0 0 14.58 14.58 0 11.22 NA 0.91 2.19

90

Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Single report Single report Controversy with other publications Controversy with other publications Single report Single report Single report Single report Confirmed in another publication Confirmed in another publication Single report Single report Confirmed in another publication Confirmed in another publication Confirmed in three other publications Confirmed in two other publications Confirmed in two other publications Single report Confirmed in another publication v 1.0


Cancer

Ref

WAF1 Comments

234 TAC 254 ATC 175 CGC

CAC GAC ins3a

Tyr Ile Arg

His Asp InF

DMD SM SM

RAJI RAMOS SG568

25 3 1

Burkitt lymphoma Burkitt lymphoma Burkitt lymphoma

161 44 447

158 CGC

CAC

Arg

His

SM

ST486

105

Burkitt lymphoma

44

158 CGC

CAC

Arg

His

DMU

ST486

105

Burkitt lymphoma

2249

239 AAC

GAC

Asn

Asp

DMU

ST486

53

Burkitt lymphoma

2249

Some publications described only one of the two mutations Confirmed in another publication Some publications described only 0 one of the two mutations NR Confirmed in three other publications NA Single report Consensus based on three puublications. Controversy with other publications Second mutation found 8.78 by COSMIC Controversy with other publications. 8.78 Excluded from the consensus Controversy with other publications. 20.19 Excluded from the consensus

289 136 301 248 319

CAC ins1a DEL1 CAG TAG

Leu Gln Pro Arg Lys

His Fs. Fs. Gln Stop

SM SM SM SM SM

CG3 K-562 KCL-22 KYO-1 LAMA-84

2 1 2 883 5

Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia Chronic Myelocytic Leukemia

356 269 150 150 2249

87.06 NA NA 0 NA

274 GTT

GAT

Val

Asp

SM

OCIM2

1.32

175 CGC 282 CGG 232 ATC

CAC TGG del11c

Arg Arg Ile

His Trp Fs.

DMD DMD SM

273 CGT

TGT

Arg

Cys

147 GTT

GGT

Val

241 TCC 278 CCT 272 GTG

TTT TCT GAG

Ser Pro Val

CTC CAA CCA CGG AAG


Single report Confirmed in another publication Single report Single report Mutation in COSMIC database

8

Erythroleukemia

12

CO CO HD-MY-Z

1187 600 1

Hodgkin disease Hodgkin disease Hodgkin disease

55 55 2249

12.41 Single report 0.55 Single report NA Mutation in COSMIC database

SM

RPMI-8402

687

Lymphoid leukemia

163

0.91

Gly

SM

Mino

8

Mantle Cell Lymphoma

1842

10.49 Single report

Phe Ser Glu

DMU DMU SM

MCC13 MCC13 MCC14/2

6 87 12

Merkel cell carcinoma Merkel cell carcinoma Merkel cell carcinoma

2088 2088 2088

0 Single report 0.34 Single report 11.52 Single report

91

Single report

Single report

v 1.0


Cancer

Ref

WAF1 Comments

Multiple Myeloma

1363

NA

Single report

0

Single report

255 ATC

del6b

Ile

InF

SM

MEF-1

2

248 CGG

CAG

Arg

Gln

SM

SKM-1

883

Myelodysplastic synd.

1363

175 CGC 251 ATC

Arg Ile

His Fs.

SM SM

KY821 TF-1

1187 5

Myeloid leukemia Myeloid leukemia

126 126

Arg

Fs.

SM

THP-1

1

Myeloid leukemia

126

NA

Confirmed in another publication

Val

Fs.

SM

U937

2

Myeloid leukemia

126

NA

Single report

261 AGT

CAC DEL1B DEL26 A DEL46 A DEL99 A

Ser

InF

SM

UT7

1

Myeloid leukemia

126

NA

Single report

273 132 143 337 261 184 286 285 161 126 176 181 282

CGT AAG GTG CGC AGT GAT GAA GAG GCC TAC TGC CGC CGG

CAT AAC ATG CTC ACT TAT AAA AAG ACC AAC TAC TGC TGG

Arg Lys Val Arg Ser Asp Glu Glu Ala Tyr Cys Arg Arg

His Asn Met Leu Thr Tyr Lys Lys Thr Asn Tyr Cys Trp

SM SM SM SM SM SM SM SM SM SM SM SM SM

ARH-77 EJM HD-70 KMS-12-PE L-363 LB 831 LP-1 RPMI-8226 U266 XG-1 XG-2 XG-4 XG-5

780 33 34 9 1 5 86 165 75 6 88 28 600

Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma Myeloma

2249 98 1004 2249 2249 98 2249 98 98 98 98 98 98

1.01 10.49 10.31 10.45 96.18 58.13 11.07 0.58 13.25 12.03 14.82 26.1 0.55

248 CGG

TGG

Arg

Trp

SM

KHYG-1

728

NK-Leukemia

1125

0

237 ATG 248 CGG 248 CGG

ATA CAG CAG

Met Arg Arg

Ile Gln Gln

DMD DMD SM

OCI-Ly4 OCI-Ly4 WSU-NHL

123 883 883

Non-Hodgkin's Lymphomas Non-Hodgkin's Lymphomas Non-Hodgkin's Lymphomas

517 517 2249

0.43 0 0

37 TCC

CCC

Ser

Pro

MM

1


1703

65.94 Single report

175 CGC

CAC

Arg

His

MM

1187


1703

12.41 Single report

174 AGG 172 GTT


CCRF-CEMVLB100 CCRF-CEMVLB100

92

12.41 wt in COSMIC NA Single report

Mutation in COSMIC database Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database

v 1.0


248 196 360 196 256 259 260 272 273 237 244 111 248 306 11 248 248 273 248 267 273 278 175 176 196

CGG CGA GGG CGA ACA GAC TCC GTG CGT ATG GGC CTG CGG CGA GAG CGG CGG CGT CGG CGG CGT CCT CGC TGC CGA

CAG TGA del1a TGA GCA GGC GCC ATG CAT AGG TGC GTG CAG TGA CAG CCG CAG TGT CAG CTG CAT TCT GGC TAC TGA


Arg Arg Gly Arg Thr Asp Ser Val Arg Met Gly Leu Arg Arg Glu Arg Arg Arg Arg Arg Arg Pro Arg Cys Arg

Gln Stop Fs. Stop Ala Gly Ala Met His Arg Cys Val Gln Stop Gln Pro Gln Cys Gln Leu His Ser Gly Tyr Stop

MM DMU DMU MM MM MM MM SM SM DMU DMU SM SM SM MM MM MM SM MM MM MM SM SM SM SM

CCRF-CEMVLB100 J-RT3-T3-5 J-RT3-T3-5 JURKAT JURKAT JURKAT JURKAT Loucy MOLT-13 MOLT-16 MOLT-16 MOLT-4 MOLT-4 MOLT-4 P12-ICHIKAWA P12-ICHIKAWA P12-ICHIKAWA PF-382 SUP-T1 SUP-T1 SUP-T1 ATL1K HATL MT1 HUT78

883 241 2 241 9 6 4 105 780 8 53 1 883 160 10 23 883 687 883 1 780 87 27 88 241

Cancer

Ref

WAF1 Comments

T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell Acute Lymphoblastic Leu T-cell leukemia T-cell leukemia T-cell leukemia T-cell Lymphoma

1703 2249 2249 3 3 3 3 1510 2249 3 3 2242 27 2249 2249 2249 2249 2249 2249 2249 2249 82 323 82 3

0 NA NA NA 10.79 17.27 66.25 8.79 1.01 5.71 0 26.49 0 NA 67.48 6.51 0 0.91 0 3.41 1.01 0.34 10.52 14.82 NA

93

Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Mutation in COSMIC database wt in COSMIC wt in COSMIC Controversy with other publications Controversy with other publications Controversy with other publications Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report

v 1.0

Melanoma Table I : cell lines with wt p53 Cell line A375 C32TG HS939 HS944T K2 K4 K11 K19 K23 K25 MEL-JUSO MeWo MGH-BO-1 MGH-ST-1 MGH-MC-1 MGH-QU-1 MGH-TH-1 MJM MM455 MM608 RU SK-MEL-23 SK-MEL-28 SK-MEL-30 SK-MEL-63 SK-MEL-93 SK-NEL-119 SK-MEL-147 Swift WM1205 WM115 WM239A WM35

ATCC CRL-1619

Ref 1006

CRL-7690 CRL-7693

HTB-65

HTB-72

CRL-1675

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) no data Cell line p53 Handbook

94

v 1.0


AAC CGG ATG TAC GAA CCT TAC GGT TCC CGC TCC CGA TGT GTT AGG CGG CAT TGC CCT AAC TGT CGA AAC CGG GGA GAA GAA CAG TTC CGA AGA CGG CGC TAC

ACC TGG AGG CAC AAA TCT TGC AGT TGC CAC TTC CAA TAT TTT GGG TGG CGT CGC CGT ATC del2b TGA AAA TGG GAA AAA AAA TAG TTT TGA GGA CAG CAC TGC

Asn Arg Met Tyr Glu Pro Tyr Gly Ser Arg Ser Arg Cys Val Arg Arg His Cys Pro Asn Cys Arg Asn Arg Gly Glu Glu Gln Phe Arg Arg Arg Arg Tyr

Thr Trp Arg His Lys Ser Cys Ser Cys His Phe Gln Tyr Phe Gly Trp Arg Arg Arg Ile Fs. Stop Lys Trp Glu Lys Lys Stop Phe Stop Gly Gln His Cys

DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU SM DMU SM SM SM SM SM SM

8823 8823 136-2 1402P 14362M 16396M 17697M 20842P 2211M 30966M 4686M 518A2 607B A2058 A4-FUK BE CHL-1 COLO-800 DX-3 HMV-II IGR39D IPC-298 IST-MEL1 IST-MEL1 M14 MeWo MeWo MeWo MeWo MeWo MLMA MM229 MM386 MRI-H-221

6 728 13 13 73 87 133 12 3 1187 30 38 98 32 52 728 86 11 39 2 6 306 4 728 74 73 73 25 4 74 40 883 31 140

Cancer

Ref

WAF1 Comments

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

1158 1158 1158 1927 1927 1927 1927 1927 1927 1927 1927 1158 1158 2249 2249 2068 2249 2249 347 2249 1158 2249 2249 2249 1018 2249 1076 2249 2019 2019 2249 1158 1158 1023

0.12 0 2.86 14.28 0.31 0.34 2.14 51.53 5.32 12.41 12.47 2.19 14.58 0.84 0.17 0 10.15 0.86 0.72 0.92 NA NA 54.76 0 0 0.31 0.31 NA NR NA 21.11 0 67.3 18.3


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Mutation in COSMIC database Single report Single report Single report v 1.0

95


Cancer

Ref

WAF1 Comments 0.48 NA NR 69.34 13.83 51.83 111.2 5 90.77 85.98 NA NA 0 12.61 0.31 1.68 1.01 NA 53.77 91.58 11.24 NA 1.21 0 NR

241 166 75 76 104 155

TCC TCA CCT GCA CAG ACC

CCC TGA CCC ACA CAC AGC

Ser Ser Pro Ala Gln Thr

Pro Stop Pro Thr His Ser

SM SM MM MM MM MM

NZM4 RPMI-7951 SK-MEL-110 SK-MEL-110 SK-MEL-110 SK-MEL-110

11 18 1 2 2 5

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

1638 1570 2019 2019 2019 2019

289 296 61 104 125 245 145 258 267 273 283 340 295 195 177 220 241 278

CTC CAC GAT CAG ACG GGC CTG GAA CGG CGT CGC ATG CCT ATC CCC TAT TCC CCT

TTC TAC GTT TAG del2b AGC CGG AAA TGG CAT del2c ATA GCT ACC del18c TGT TTC TTT

Leu His Asp Gln Thr Gly Leu Glu Arg Arg Arg Met Pro Ile Pro Tyr Ser Pro

Phe Tyr Val Stop Fs. Ser Arg Lys Trp His Fs. Ile Ala Thr InF Cys Phe Phe

MM MM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM

SK-MEL-110 SK-MEL-110 SK-MEL-136 SK-MEL-146 SK-MEL-149 SK-MEL-2 SK-MEL-28 SK-MEL-29 SK-MEL-3 SK-MEL-30 SK-MEL-30 SK-MEL-37 SK-MEL-93/4 UISO-MEL-11 UISO-MEL-23 WM164 WM852 WM983

5 7 1 18 1 440 10 73 37 780 1 1 1 90 1 336 101 9

Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma Melanoma

2019 2019 2019 2019 2019 1018 1018 2019 2249 2249 2249 2019 2019 2020 2020 233 233 233


Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report Single report

v 1.0 96

Non Small Cell Lung Cancer Table I : cell lines with wt p53 Cell line A-549 LU99 LU99A LU99B A427 Ma-12 Ma-17 Ma-26 NCI-H460 NCI-H726 NCI-H838 NCI-H1385 NCI-H1568 NCI-H1570 NCI-H1653 NCI-H1725

ATCC CCL-185

HTB-53

HTB-177 CRL-5844 CRL-5867 CRL-5876

NCI-H1944 NCI-H2023 NCI-H2030 NCI-H2077 NCI-H2126 NCI-H2347 NCI-H1395 NCI-H1563 NCI-H1650 NCI-H1666 NCI-H1975 NCI-H2170 NCI-H2228 NCI-H2342 NCI-H2347 NCI-H810

Ref. 1006 1006 1006 1006 1382 1382 1382 1382 678 678 678 678 678 678 678 678

CRL-5907 CRL-5912 CRL-5914 CRL-5919 CRL-5925 CRL-5942 CRL-5868 CRL-5875 CRL-5883 CRL-5885 CRL-5908 CRL-5928 CRL-5935 CRL-5941 CRL-5970 CRL-5816

678 678 678 678 678 678 2249 2249 678 678 2249 2249 2249 2249 2249 678

Table II : cell lines with p53 gene deletion or rearrangement Cell line NCI-H1299 H-358 Calu-1

ATCC CRL-5803 CRL5807 HTB-54

Reference

Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) Cell line NCI-H648 NCI-H1710 NCI-H920 NCI-H1792


ATCC CRL-5834

Mutation intron7 intron8 intron5 c.672+1G>A

CRL-5850 CRL-5895

97

reference 92 92 92 92

v 1.0


TGT CCT CCC GAC AGA TGC AGA ATG CGA CGC GAG GGT GTG GAG CGT CGG GAA AAG GGC TAC CGC AAG AGG ATG TCC GTG CGG GTG GAG CGT CGG CGG GGC CGG CGC

TGA TCT del1 GGC TGA TTC AAA ATT TGA CAC TAG del111 GTT TAG TGT CTG TAA del1c GTC TGC CTC AAT AGT ATT TGC TTG CTG ATG CAG CAT TGG CAG GTC TGG CAC


Cys Pro Pro Asp Arg Cys Arg Met Arg Arg Glu Gly Val Glu Arg Arg Glu Lys Gly Tyr Arg Lys Arg Met Ser Val Arg Val Glu Arg Arg Arg Gly Arg Arg

Stop Ser Fs. Gly Stop Phe Lys Ile Stop His Stop InF Val Stop Cys Leu Stop Fs. Val Cys Leu Asn Ser Ile Cys Leu Leu Met Gln His Trp Gln Val Trp His

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

866MT ABC-1 ACC-LC-319 ACC-LC94 CAEP CAL-12T Ca-Lu-1 CALU-3 CALU6 CMRC-LCD EBC-1 EKVX EKVX EKVX GLCA2 GLCP1 GLCP1 HCC1438 HCC2108 HCC2279 HOP-92 IGR-Heu KNS-62 LC-1-SQ LC-2-ad LCLC-103H LCMS LK-2 LU-65 LX-1 LXF-289 MA1 MA-10 MA14 MA2

6 87 2 16 14 52 78 52 241 1187 25 1 2 46 687 124 7 1 65 133 25 23 389 52 36 13 124 105 10 780 728 883 84 600 1187

Cancer

Ref

WAF1 Comments

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

16 1382 1976 1976 1393 2249 1081 2249 16 1382 1382 1018 2249 2249 1386 1386 1386 1327 1327 1327 1018 2195 2249 364 2249 2249 1382 1006 1382 1081 2249 1382 1382 1574 1382

NA 0.34 NA 12.06 NA 10.37 0.46 0.43 NA 12.41 NA NA NR NA 0.91 0 NA NA 8.39 2.14 13.16 10.49 12.42 0.43 0 2.73 0 8.79 67.48 1.01 0 0 0 0.55 12.41

98

Single report Single report Single report Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Single report Single report Single report Single report Single report Single report Confirmed in COSMIC database Single report Mutation in COSMIC database Confirmed in another publication Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Confirmed in another publication Single report Single report Confirmed in another publication v 1.0


Cancer

Ref

WAF1 Comments 11.86 0.43 NA 0 0 1.01 NA NA 0 2.14 59.68 0.58 144.0 3 NA 6.4 0 NA NA 0 NA NA 0 NA 0.86 12.41 NA 14.82 0.58 0.86 13.79 NR 0 9.06 NA

337 237 121 245 245 273 239 298 248 234 224 285

CGC ATG TCT GGC GGC CGT AAC GAG CGG TAC GAG GAG

TGC ATA del1c TGC GTC CAT ins1b TAG TGG TGC GAC AAG

Arg Met Ser Gly Gly Arg Asn Glu Arg Tyr Glu Glu

Cys Ile Fs. Cys Val His Fs. Stop Trp Cys Asp Lys

SM SM SM SM SM SM SM SM SM SM SM SM

MA-24 MA25 MA-29 MA3 MA36 NCI-H1155 NCI-H125 NCI-H1264 NCI-H1284 NCI-H1304 NCI-H1334 NCI-H1355

19 123 1 86 84 780 1 71 728 133 10 165

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

1382 1382 1382 1382 1574 92 678 92 678 678 92 92

47 144 141 267 89 34 282 298 298 248 144 273 175 35 176 285 273 242 247 248 157 209

CCG CAG TGC CGG CCC CCC CGG GAG GAG CGG CAG CGT CGC TTG TGC GAG CGT TGC AAC CGG GTC AGA

CTG TAG TGG CCG del1 ins1 CCG TAG TAG CTG TAG CTT CAC ins1b TAC AAG CTT TTC AAT TGG TTC TGA

Pro Gln Cys Arg Pro Pro Arg Glu Glu Arg Gln Arg Arg Leu Cys Glu Arg Cys Asn Arg Val Arg

Leu Stop Trp Pro Fs. Fs. Pro Stop Stop Leu Stop Leu His Fs. Tyr Lys Leu Phe Asn Trp Phe Stop

SM SM SM SM SM DMU SM SM DMU SM SM SM SM SM SM SM SM SM SM SM SM SM

NCI-H1373 NCI-H1404 NCI-H1435 NCI-H1437 NCI-H1466 NCI-H157 NCI-H157 NCI-H157 NCI-H157 NCI-H1573 NCI-H1581 NCI-H1623 NCI-H1628 NCI-H1648 NCI-H1651 NCI-H1703 NCI-H1734 NCI-H1755 NCI-H1770 NCI-H1770 NCI-H1781 NCI-H1793

3 53 11 19 3 1 21 71 71 124 53 147 1187 1 88 165 147 88 39 728 177 14

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

106 92 92 92 92 1382 2021 92 1382 92 92 92 678 92 92 92 92 92 2249 2249 92 92


99

Single report Confirmed in another publication Single report Confirmed in another publication Single report Confirmed in two other publications Single report Single report Single report wt in COSMIC Single report Confirmed in another publication Controversy with other publications Single report Single report Confirmed in another publication Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Single report wt in COSMIC Single report Single report wt in COSMIC Single report Controversy with other publications wt in COSMIC Single report Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications

v 1.0


Cancer

Ref

WAF1 Comments 1.01 0.86 0.43 NA NA 14.19 0.86 11.71 14.19 0 9.06 0 71.32 22.88 NA 13.25 8.19 55.15 8.39 0.28 1.01 0 12.42 12.42 10.87

273 273 237 209 291 242 273 262 242 220 157 248 16 176 62 161 158 309 154 246 273 248 249 249 259

CGT CGT ATG AGA AAG TGC CGT GGT TGC TAT GTC CGG CAG TGC GAA GCC CGC CCC GGC ATG CGT CGG AGG AGG GAC

CAT CTT ATA TGA TAG TGG CTT GTT TGG TCT TTC TGG CTG TTC TAA ACC CTC GCC GTC ATC CAT CTG AGC AGC GTC

Arg Arg Met Arg Lys Cys Arg Gly Cys Tyr Val Arg Gln Cys Glu Ala Arg Pro Gly Met Arg Arg Arg Arg Asp

His Leu Ile Stop Stop Trp Leu Val Trp Ser Phe Trp Leu Phe Stop Thr Leu Ala Val Ile His Leu Ser Ser Val

SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM DMU DMU

NCI-H1793 NCI-H1838 NCI-H1869 NCI-H1904 NCI-H1915 NCI-H1993 NCI-H2009 NCI-H2030 NCI-H2073 NCI-H2086 NCI-H2087 NCI-H2106 NCI-H2122 NCI-H2122 NCI-H2126 NCI-H2250 NCI-H226 NCI-H226 NCI-H2291 NCI-H23 NCI-H2405 NCI-H322 NCI-H324 NCI-H324 NCI-H324

780 147 123 14 9 16 147 14 16 20 177 728 1 191 8 75 92 1 65 6 780 124 34 34 21

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

2249 2249 678 92 92 2249 92 2249 92 106 92 106 2249 2249 2249 92 92 1018 2249 17 2249 92 678 92 92

158 146 191 245 164 215 217 248 102

CGC TGG CCT GGC AAG AGT GTG CGG ACC

CTC TGA del1a TGC AAT ATT TTG CTG del1

Arg Trp Pro Gly Lys Ser Val Arg Thr

Leu Stop Fs. Cys Asn Ile Leu Leu Fs.

SM SM SM SM SM SM DMU DMU SM

NCI-H441 NCI-H520 NCI-H522 NCI-H596 NCI-H650 NCI-H661 NCI-H676 NCI-H676 NCI-H679

92 51 8 86 8 25 3 124 1

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

92 92 92 92 92 92 92 92 92


100

Controversy with other publications Mutation in COSMIC database Single report Single report Single report Mutation in COSMIC database Single report Mutation in COSMIC database Single report Single report Confirmed in another publication Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Mutation in COSMIC database Confirmed in another publication Mutation in COSMIC database Confirmed in another publication Controversy with other publications Controversy with other publications Controversy with other publications Confirmed in another publication. wt 8.19 in COSMIC NA wt in COSMIC NA Single report 0 Confirmed in another publication 22.27 Single report 8.11 wt in COSMIC 50.18 Single report 0 Single report NA Single report

v 1.0


TGC ATC CGG ACA GAG AGG GGG GGC GGG CGG CGG CGG CAT CGG CAG GGC TTC CGC CAT

Cancer

Ref

WAF1 Comments 15.16 NA 0 7.25 0.58 12.42 25.23 0 25.23 0 0 0.55 3.12 0 NA 0 12.55 8.19 10.15

TGG ins9c CCG CCA AAG AGT GTG TGC GTG TGG CAG TGG CGT CAG TAG TGC TGC CTC CGT

Cys Ile Arg Thr Glu Arg Gly Gly Gly Arg Arg Arg His Arg Gln Gly Phe Arg His

Trp InF Pro Pro Lys Ser Val Cys Val Trp Gln Trp Arg Gln Stop Cys Cys Leu Arg

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

NCI-H720 NCI-H727 NCI-H738 NCI-H820 NCI-H854 OG56 PC-1 PC-10 PC-13 PC-14 PC-14 PC-3 PC-7 PC-9 PERF-LC-AI RAL RERF-LOCK SK-LC-6 SKLU1

19 1 19 10 165 389 3 86 3 728 883 600 69 883 18 53 9 92 86

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

92 1707 678 106 92 1976 1382 364 1382 1382 2242 364 1382 364 1976 1393 1382 1976 16

280 AGA

AAA

Arg

Lys

SM

SK-MES-1

78

Lung (NSCLC)

1081

298 277 167 347 175 175 204

TAG TTT TAG ACC del1 CAC TAG

Glu Cys Gln Ala Arg Arg Glu

Stop Phe Stop Thr Fs. His Stop


SK-MES-1 SW1271 SW900 U-1752 U-1810 VRMC-LCD Y-ML-1B

71 48 40 3 3 1187 46

Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC) Lung (NSCLC)

303 16 2249 2021 2021 1976 1867

GAG TGT CAG GCC CGC CGC GAG


101

wt in COSMIC Single report Single report Single report Single report Single report Single report Single report Single report Controversy with other publications Controversy with other publications Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Controversy with other publications. 0.46 Excluded from the cosnensus Consensus based on three publications. Controversy with other NA publications 0.31 Single report NA Mutation in COSMIC database 24.06 Single report NA Single report 12.41 Single report NA Single report

v 1.0

Pancretic Tumors Table I : cell lines with wt p53 Cell line PK-45

ATCC

Reference 1556

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) No data


102

v 1.0


Cancer

Ref

176 TGC 151 CCC

AGC TCC

Cys Pro

Ser Ser

SM SM

8902 8988S

17 92

Pancreatic cancer Pancreatic cancer

428 428

134 TTT

del1a

Phe

Fs.

SM

AsPC-1

4

Pancreatic cancer

397

273 CGT 197 GTG 275 TGT

CAT TTG TAT

Arg Val Cys

His Leu Tyr

SM SM SM

ASPC-1 BI BJ

780 5 87


132 1653 1653

220 TAT

TGT

Tyr

Cys

SM

BxPC-3

336

Pancreatic cancer

176

159 273 242 175 272 262 151 266 193 152 249 181 282 255 130 306 132 273 248 209 282

GTC CAT CGC CAC TTG GTT TCC GAA CGT del1a ATG CAC TGG ACC GTC TGA AGG TGT TGG del2b TGG

Ala Arg Cys Arg Val Gly Pro Gly His Pro Arg Arg Arg Ile Leu Arg Lys Arg Arg Arg Arg

Val His Arg His Leu Val Ser Glu Arg Fs. Met His Trp Thr Val Stop Arg Cys Trp Fs. Trp

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

CAPAN-1 capan-2 CFPAC-1 FAMPAC Ger H-74 HPAF-II HPC-Y19 HPC-Y21 HPC-YO Hs 700T HS766T HuP-T3 HuP-T4 IMIM-PC-1 IMIM-PC-2 MCC1 MDA-Panc3 MIA PaCa-2 MZ1-PC MZ-PC-2

50 780 14 1187 39 14 92 74 86 3 64 34 600 19 22 160 51 687 728 14 600

Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer

177 132 397 2116 1653 1689 177 440 440 440 132 132 2249 2249 177 177 2000 1653 177 2249 177

GCC CGT TGC CGC GTG GGT CCC GGA CAT CCG AGG CGC CGG ATC CTC CGA AAG CGT CGG AGA CGG


WAF1 Comments 13.27 Single report 0.85 Single report Consensus based on four publications. Controversy with other NA publications Controversy with other publications. 1.01 Excluded from the consensus 12.36 Single report 0.42 Single report Consensus based on six publications. A single publication decsribes a second mutation (neutral 1.21 mutation) Confirmed in two other publications. 6.91 wt in COSMIC 1.01 wt in COSMIC 0 Single report 12.41 Single report 7.06 Single report 11.71 Single report 0.85 Confirmed in another publication 0 Single report 10.15 Single report NA Single report 0 Confirmed in another publication 34.07 Single report 0.55 Mutation in COSMIC database 11.89 Mutation in COSMIC database 8.89 Single report NA Single report 14.1 Single report 0.91 Single report 0 Confirmed in four other publications NA Mutation in COSMIC database 0.55 Single report

v 1.0 103


Cancer

Ref

WAF1 Comments

135 TGC 282 CGG 220 TAT

TGG GGG TGT

Cys Arg Tyr

Trp Gly Cys

SM SM SM

MZ-PC-4 PAN-03-JCK Panc 89

25 48 336


177 1556 176

12.8 0.44 1.21

273 CGT

CAT

Arg

His

SM

Panc-1

780

Pancreatic cancer

178

1.01

273 255 176 175 176 237 237 167 213 255 132 280 175 282 179 175 238 245 273 220 273 179

TGT AAC AGC CAC AGC ATA ATA TAG TGA ACC CAG AAA CAC TGG CGT CAC TAT AGC CAT TGT CAT CGT

Arg Ile Cys Arg Cys Met Met Gln Arg Ile Lys Arg Arg Arg His Arg Cys Gly Arg Tyr Arg His

Cys Asn Ser His Ser Ile Ile Stop Stop Thr Gln Lys His Trp Arg His Tyr Ser His Cys His Arg

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

Panc-1 PANC-10-05 Panc-TU-I PC PC-44 PCI-55 PK-1 PK-8 PK-9 PL 45 PSN1 PT45 RWP-2 SK-PC-1 SK-PC-3 SNU-213 SNU-494 SU86.86 Suit-2 T3M4 UK Pan-1 YAPC

687 6 17 1187 17 123 123 40 306 19 14 78 1187 600 146 1187 98 440 780 336 780 146

Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer Pancreatic cancer

177 2249 176 1653 428 1556 1556 1556 1556 965 38 178 177 177 177 1491 1491 397 1653 1653 659 2249

0.91 6.97 13.27 12.41 13.27 0.43 0.43 NA NA 11.89 10.86 0.46 12.41 0.55 13.02 12.41 14.58 0 1.01 1.21 1.01 13.02

CGT ATC TGC CGC TGC ATG ATG CAG CGA ATC AAG AGA CGC CGG CAT CGC TGT GGC CGT TAT CGT CAT


Single report Single report Single report Consensus based on four publications. Controversy with one publication Controversy with other publications. Excluded from the cosnensus Mutation in COSMIC database Confirmed in another publication Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Confirmed in another publication Single report Single report Single report Mutation in COSMIC database

v 1.0 104

Prostate Tumors Table I : cell lines with wt p53 Cell line LNCaP-FGC MDA-PCa2A PC-436C PC-82

ATCC CRL-1740

Reference 2249 1991 1991 59

Table II : cell lines with p53 gene deletion or rearrangement Cell line PC-3

ATCC CRL-1435

Reference



105

v 1.0

Table IV: cell lines with missense or small frameshift mutations. Pos. WT Mut AA Mut Comp Name NB 138 331 196 273 331

GCC CAG CGA CGT CAG

Cancer

Ref

WAF1 Comments NA 67.76 NA 1.01 67.76

del1 CGG TGA CAT CGG

Ala Gln Arg Arg Gln

Fs. Arg Stop His Arg

SM SM SM DMU DMU

1LN 22Rv1 ARCaP CWR-R1 CWR-R1

5 3 241 780 3

Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca.

220 1991 1991 1991 1991

223 CCT

CTT

Pro

Leu

DMD

DU-145

5

Prostate ca.

59

274 248 138 175 152 152 273 196 138 279 126 248

TTT TGG del1 CAC CCA CCA CAT TGA del1 GAG TAG TGG

Val Arg Ala Arg Pro Pro Arg Arg Ala Gly Tyr Arg

Phe Trp Fs. His Pro Pro His Stop Fs. Glu Stop Trp

DMD SM SM SM SM DMU DMU SM SM SM SM SM

DU-145 DuCaP Dupro LAPC-4 LNCaP-ATCC LNCaP-GW LNCaP-GW MDA-PCa1 PC-3 PSK-1 TSU VCaP

32 728 5 1187 13 13 780 241 5 42 14 728

Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca. Prostate ca.

59 1991 220 1991 316 316 316 1991 59 1991 59 1991

GTT CGG GCC CGC CCG CCG CGT CGA GCC GGG TAC CGG


Single report wt in COSMIC Single report Single report Single report Consensus based on three publications. Controvery withy other publications (only one of the two 8.54 mutations is found) Consensus based on three publications. Controvery withy other publications (only one of the two 0.84 mutations is found) 0 Single report NA Single report 12.41 Single report NR Confirmed in another publication NR Single report 1.01 Single report NA Single report NA Confirmed in two other publications 0.27 Single report NA Single report 0 Single report

v 1.0 106

Sarcoma Table I : cell lines with wt p53 Cell line FPBH SAL-2 STA-ET-1 WE-68 VH-64 STA-ET-6 TC 252

ATCC

Reference Ewing sarcoma Ewing sarcoma Ewing sarcoma Ewing sarcoma Ewing sarcoma Ewing sarcoma Ewing sarcoma

U2OS* SJSA**

HTB-96 CRL-2098

Osteosarcoma Osteosarcoma

HT1080

CCL-121

Fibosarcoma Undifferentiated sarcoma

A-204

* Mdm2 overexpression **Mdm2 amplification Table II : cell lines with p53 gene deletion or rearrangement Cell line Saos-2 SK-N-MC

ATCC HTB-85 HTB-10

Reference Osteosarcoma Ewing sarcoma



107

v 1.0


Cancer

Ref

WAF1 Comments

Angiosarcoma (hepatic)

1353

21.26 Single report

179 CAT

CTT

His

Leu

SM

ASL5

38

175 CGC

CAC

Arg

His

SM

CH-1

1187

Chondrosarcoma

1373

12.41 Single report

194 175 176 273 285 118 283 181 164 273 273 273 176 141 176 176 273 277 273 176

CGT CAC TTC TGT AAG ins2 DEL1C CAC GAG TGT TGT CAT TTC TAC TTC TTC TGT TAT TGT TTC

Leu Arg Cys Arg Glu Thr Arg Arg Lys Arg Arg Arg Cys Cys Cys Cys Arg Cys Arg Cys

Arg His Phe Cys Lys Fs. Fs. His Glu Cys Cys His Phe Tyr Phe Phe Cys Tyr Cys Phe

SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

19 58 59 63 64 A673 ES-1-OT EW-13 EW-24 IARC-EW2 RD-ES RM 82 SCMC-ES-2 SCMCS-ES-1 SK-ES-1 SK-PN-DW SMB STA-ET-2.1 STA-ET-7.1 W-ES

66 1187 191 687 165 1 4 34 25 687 687 780 191 103 191 191 687 28 687 191

Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's Ewing's

328 328 328 328 328 226 245 2249 2249 226 226 226 245 245 226 2249 226 226 226 245

10.61 12.41 22.88 0.91 0.58 NA NA 34.07 12.39 0.91 0.91 1.01 22.88 9.84 22.88 22.88 0.91 0.68 0.91 22.88

213 CGA

TGA

Arg

Stop SM

SW684

306

Fibrosarcoma

2249

NA

215 237 245 245 175 175

ins1 AAG AGC AGC CAC CAC

Ser Met Gly Gly Arg Arg

Fs. Lys Ser Ser His His

LMS6-93 SK-LMS-1 SK-LMS-1 SK-LMS-1 SK-UT-1 SK-UT-1

3 11 440 440 1187 1187

Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma Leyomyosarcoma

1552 2198 14 2198 2249 14

NA 14.24 0 0 12.41 12.41

CTT CGC TGC CGT GAG ACA CGC CGC AAG CGT CGT CGT TGC TGC TGC TGC CGT TGT CGT TGC

AGT ATG GGC GGC CGC CGC

SM DMU SM DMU DMU SM


Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma Sarcoma

Single report Single report Single report Single report Single report wt in COSMIC Single report Mutation in COSMIC database Mutation in COSMIC database Single report Confirmed in another publication Single report Single report Single report Confirmed in another publication Mutation in COSMIC database Single report Single report Single report Single report Mutation in COSMIC database Single report Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications Controversy with other publications v 1.0

108


Cancer

Ref

WAF1 Comments

248 CGG

CAG

Arg

Gln

DMU

SK-UT-1

883

Leyomyosarcoma

2249

0

251 ATC

AAC

Ile

Asn

SM

SW872

21

Liposarcoma

2249

8.75

Mutation in COSMIC database

156 306 76 286 250 245

CCC TGA ins1a AAA del8 AGC

Arg Arg Ala Glu Pro Gly

Pro Stop Fs. Lys Fs. Ser

SM SM SM SM SM SM

HOS HOSM-2 HuO-3N1 OH RIT-1B RIT2

38 160 1 86 1 440

Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma Osteosarcoma

25 1920 2249 272 65 65

8.22 NA NA 11.07 NA 0

Confirmed in another publication Single report Mutation in COSMIC database Single report Single report Single report

219 CCC

del4

Pro

Fs.

SM

ctr

NA

248 273 280 156 316 317 326

TGG TGT AGT CCC CCT TAG TAA

Arg Arg Arg Arg Pro Gln Glu

Trp Cys Ser Pro Pro Stop Stop


RD Rh30 RH30 RMS GCT GCT S-117

CGC CGA GCA GAA CCC GGC

CGG CGT AGA CGC CCC CAG GAA

1

Rhabdomyosarcoma

97

728 687 14 38 6 25 9

Rhabdomyosarcoma Rhabdomyosarcoma Rhabdomyosarcoma Rhabdomyosarcoma Sarcoma Sarcoma Sarcoma

14 1190 97 14 2249 2249 2249


Controversy with other publications

Single report Confirmed in another publication. wt 0 in COSMIC 0.91 Controversy with other publications 20.55 Controversy with other publications 8.22 Single report NR Mutation in COSMIC database NA Mutation in COSMIC database NA Mutation in COSMIC database

v 1.0 109


Cancer

Ref

WAF1 Comments

126 249 110 152 245 306 281 245 155 245 241

TAC AGG CGT CCG GGC CGA GAC GGC ACC GGC TCC

TGC AGT del1 ins1 TGC TGA CAC GTC CCC TGC TTC

Tyr Arg Arg Pro Gly Arg Asp Gly Thr Gly Ser

Cys Ser Fs. Fs. Cys Stop His Val Pro Cys Phe

SM SM SM SM SM SM SM SM SM SM SM

ACC-LC-172 ACC-LC-48 ACC-LC-49 ACC-LC-80 COLO-668 COR-L51 COR-L96CAR CPC-N DMS-153 DMS-273 DMS-53

17 389 3 8 86 160 41 84 20 86 101

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

1976 1976 1976 1976 2249 2249 2249 2249 1953 2249 2249

11.62 12.42 NA NA 0 NA 0.66 0 7.8 0 0

278 237 273 282 53 146 175 158 132 332 326 218 144 280 193 294 294 278 278 244 248 157 342

CCT ATG CGT CGG TGG TGG CGC CGC AAG ATC GAA GTG CAG AGA CAT GAG GAG CCT CCT GGC CGG GTC CGA

del2c ATA CTT TGG TGA TAG CAC CTC GAG del1c TAA GGG CCG GGA CTT TAG del1a CTT CTT TGC CTG TTC del1b

Pro Met Arg Arg Trp Trp Arg Arg Lys Ile Glu Val Gln Arg His Glu Glu Pro Pro Gly Arg Val Arg

Fs. Ile Leu Trp Stop Stop His Leu Glu Fs. Stop Gly Pro Gly Leu Stop Fs. Leu Leu Cys Leu Phe Fs.

SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM

DMS-79 DMS-92 GLC14 GLC28 GLC3 GLC35 GLC35 GLC36 GLC4 GLC42 GLC44 GLC45 GLC7 GLC8 HOV IST-SL2 LB647-SCLC LU-130 LU-134-A LU-135 LU-138 LU-139 LU-140

1 123 147 600 7 46 1187 92 25 4 9 11 10 40 55 54 1 84 84 53 124 177 1

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

2249 16 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 1386 2249 2249 1382 364 364 1382 364 1382

NA 0.43 0.86 0.55 NA NA 12.41 8.19 0.56 NA NA 61.46 13.75 21.11 11.02 NA NA 0.81 0.81 0 0 9.06 NA


Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database. del2 and ins 1 Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Single report Single report Single report Single report Single report v 1.0

110


CAT CGC GGC GAG GAG TCC CGT CGT AGG GGG CGC CAT CTT CCA GAG CCC CGG GGA TCC CGT CGT CGA GGC GTT CAT TAT CGG AGA CAG GTC TTT AGA TGT TAC CGG

TAT del13c AGC TAG TAG del1b TGT TGT AGT GTG del1b CAG CGT del19b TAG CAC CTG GTA TGC CTT CTT TGA CGC GAT CGT GAT CAG TGA TAG TTC TTA TGA TTT TGC GGG

His Arg Gly Glu Glu Ser Arg Arg Arg Gly Arg His Leu Pro Glu Pro Arg Gly Ser Arg Arg Arg Gly Val His Tyr Arg Arg Gln Val Phe Arg Cys Tyr Arg

Tyr Fs. Ser Stop Stop Fs. Cys Cys Ser Val Fs. Gln Arg Fs. Stop His Leu Val Cys Leu Leu Stop Arg Asp Arg Asp Gln Stop Stop Phe Leu Stop Phe Cys Gly

SM SM SM SM SM DMU SM DMU SM SM SM SM SM SM SM SM SM SM SM SM SM SM SM DMU DMU SM SM SM SM SM SM SM SM SM SM

LU-141 LU-165 MS-1 N230 N231 NCI-H1048 NCI-H1048 NCI-H1048 NCI-H1105 NCI-H1184 NCI-H1417 NCI-H1436 NCI-H1450 NCI-H146 NCI-H1514 NCI-H1607 NCI-H1618 NCI-H1672 NCI-H187 NCI-H1881 NCI-H1882 NCI-H1926 NCI-H1930 NCI-H1963 NCI-H1963 NCI-H2029 NCI-H211 NCI-H2141 NCI-H2171 NCI-H2196 NCI-H231 NCI-H2330 NCI-H250 NCI-H345 NCI-H510

128 1 72 71 71 1 687 687 389 3 2 14 66 1 5 33 124 51 36 147 147 306 20 7 69 5 883 14 53 177 4 9 48 75 48

Cancer

Ref

WAF1 Comments

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

1382 2249 2242 1382 1382 2249 22 2249 2249 22 678 678 17 2249 22 22 22 22 1712 22 678 22 2249 22 22 2249 22 22 2249 2249 9 2249 2249 2249 22

13.27 NA 0.34 NA NA NA 0.91 0.91 12.42 25.23 NA 17.51 10.61 NA NA 10.75 0 0 0 0.86 0.86 NA 7.9 11.64 3.12 0.96 0 NA NA 9.06 10.71 NA 0.31 0.7 0.44


Single report Mutation in COSMIC database Single report Single report Single report Controversy with other publications Controversy with other publications Controversy with other publications Mutation in COSMIC database Single report wt in COSMIC wt in COSMIC Single report Mutation in COSMIC database Single report Single report wt in COSMIC Single report Single report Single report wt in COSMIC wt in COSMIC Mutation in COSMIC database wt in COSMIC wt in COSMIC Mutation in COSMIC database Single report Single report Mutation in COSMIC database Mutation in COSMIC database Single report Mutation in COSMIC database Mutation in COSMIC database Mutation in COSMIC database wt in COSMIC v 1.0

111


Cancer

Ref

283 171 248 342 242 242 298 298 120

CGC GAG CGG CGA TGC TGC GAG GAG AAG

CCC TAG CAG TGA TCC TCC TAG TAG TAG

Arg Glu Arg Arg Cys Cys Glu Glu Lys

Pro Stop Gln Stop Ser Ser Stop Stop Stop


NCI-H64 NCI-H69 NCI-H719 NCI-H774 NCI-H841 NCI-H889 NCI-N417 RERF-LCMA SBC-1

35 25 883 74 19 19 71 71 2

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

2249 47 2249 22 1382 17 106 364 2249

248 278 85 259 248

CGG CCT CCT GAC CGG

CTG CGT del1 GTC CTG

Arg Pro Pro Asp Arg

Leu Arg Fs. Val Leu

SM SM SM SM SM

SBC-5 SCLC-21H SK-LC-2 U-1690 U-1906

124 39 1 21 124

Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC) Lung (SCLC)

364 2249 1976 2021 2021


WAF1 Comments 0.18 NA 0 NA 0 0 NA NA NA

Mutation in COSMIC database Confirmed in three other publications Mutation in COSMIC database Confirmed in another publication Single report Confirmed in another publication Single report Confirmed in another publication Mutation in COSMIC database Confirmed in another publication. wt 0 in COSMIC 0.72 Mutation in COSMIC database NA Single report 10.87 Single report 0 Single report

v 1.0 112

Small Cell Lung Cancer Table I : cell lines with wt p53 Cell line SBC-3 Lu24 MS-18 NCI-H209 NCI-H128 NCI-H1522 NCI-H2081 NCI-H446 NCI-H711 NCI-H748

ATCC

HTB-172 HTB-120 CRL-5874 CRL-5920 HTB-171 CRL-5836 CRL-5841

Reference 1006 1382 1382 1382 2249 2249 2249 2249 678 678

Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) Cell line NCI-H1694 NCI-H2227 NCI-H526



Mutation c.783-1G>T c.783-2A>C splice intron3

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