Description of the phenotype - PLOS

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Anxiety assay, transitions between light and dark sides of a light-dark box for males [n]. 11391. Novel open field behavior, locomotion in the center from 30-45 ...
Description of the phenotype

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Fear conditioning response, activity during third tone-shock pairing for males [units] Novel open field behavior, locomotion in the center from 0-15 min for males [cm] Novel open field behavior, locomotion in the center from 0-15 min for males [n beam breaks] Novel open field behavior, locomotion from 5-10 min [cm] Total distance travelled day 1 of novel environment [cm] Anxiety assay, untreated baseline, percentage of entries into closed arms of a plus maze for males [%] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during last 5 min [beam breaks/sec] Locomotion, distance traveled in 30 min in open field, baseline [cm] Novel open field behavior, locomotion from 0-30 min [cm] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during 10 min [beam breaks/sec] Novel open field behavior, locomotion in the center from 0-60 min for males [n beam breaks] Fear conditioning response, activity during third tone-shock pairing for males and females [units] Novel open field behavior, locomotion in the center from 0-60 min for males [cm] Novel open field behavior, locomotion from 10-15 min [cm] Novel open field behavior, locomotion from 20-25 min [cm] Fear conditioning behavior, baseline activity in apparatus for males [units] Novel open field behavior, locomotion in the center from 15-30 min for males [n beam breaks] Fear conditioning response, activity during second tone-shock pairing for males and females [units] Locomotion in both compartments of a light-dark box for males and females [cm] Novel open field behavior, locomotion in the center from 15-30 min for males [cm] Fear conditioning response, activity during second tone-shock pairing for males [units] Novel open field behavior, locomotion in the center from 0-15 min for males and females [cm] Locomotion in both compartments of a light-dark box for males [cm] Locomotion in dark compartment of a light-dark box for males and females [cm] Fear conditioning response, baseline activity in apparatus for males and females [units] Locomotion in dark compartment of a light-dark box for males [cm] Novel open field behavior, locomotion in the center from 0-15 min for males and females [n beam breaks] Novel open field behavior, locomotion from 15-20 min [cm] Fear conditioning response, activity after second tone-shock pairing for males [n beam breaks/30 sec test] Open field behavior, locomotion from 0-5 min for males and females [cm] Fear conditioning, baseline preconditioning freezing during a 2 min acclimation period prior to first pairing of conditioned stimulus (CS) and unconditioned stimulus (US) [%] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during first 5 min [beam breaks/sec] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males and females during 10 min [percentage of time] Anxiety assay, locomotion in the light compartment of a light-dark box for males and females [cm] Fear conditioning response, contextual activity for males and females [units] Anxiety assay, transitions between light and dark sides of a light-dark box for males and females [n] Anxiety assay, transitions between light and dark sides of a light-dark box for males [n] Novel open field behavior, locomotion in the center from 30-45 min for males [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during last 5 min [beam breaks/sec] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males and females during first 5 min [percentage of time] Fear conditioning response, contextual activity for males [units] Open field behavior, locomotion from 0-5 min for females [cm] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males and females during last 5 min [percentage of time] Open field behavior, locomotion from 5-10 min for males and females [cm] Anxiety assay, time in closed arms of an elevated plus maze for males [sec] Anxiety assay, untreated baseline, percent time in closed arms of a plus maze for males [%] Open field behavior, locomotion for entire test period for males and females [cm] Fear conditioning response, activity after third tone-shock pairing for females [n beam breaks/30 sec test] Fear conditioning response, activity after first tone shock pairing for males [n beam breaks/30 sec test] Anxiety assay, locomotion in the light compartment of a light-dark box for males [cm] Fear conditioning response, activity after second tone-shock pairing for males and females [n beam breaks/30 sec test] Open field behavior, locomotion from 5-10 min for males [cm]

11399 11510 11506 10916 10037 11456 12353 10416 10910 12354 11500 11913 11501 10912 10914 11393 11507 11912 11900 11511 11398 12024 11386 11898 11907 11384 12020 10913 11401 11921 10899 12352 12358 11901 11908 11905 11391 11508 12363 12356 11394 11664 12357 11924 11466 11458 11932 11659 11400 11387 11915 11410

Locomotion in zero maze for males [n beam breaks] Fear conditioning response, activity during first tone-shock pairing for males [units] Novel open field behavior, locomotion in the center from 0-60 min for males and females [n beam breaks] Locomotion in both compartments of a light-dark box for females [cm] Novel open field behavior, locomotion in the center from 30-45 min for males [cm] Open field behavior, locomotion from 10-15 min for males [cm] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old females only during last 5 min [percentage of time] Open field behavior, locomotion from 0-5 min for males [cm] Novel open field behavior, locomotion in the center from 0-60 min for males and females [cm] Open field behavior, locomotion for entire test period for males [cm] Anxiety assay, locomotion in the light compartment of a light-dark box for females [cm] Open field behavior, locomotion from 10-15 min for males and females [cm] Novel open field behavior, locomotion from 25-30 min [cm] Fear conditioning response, activity after first tone-shock pairing for males and females [n beam breaks/30 sec test] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during last 5 min [n beam breaks] Novel open field behavior, locomotion from 0-5 min [cm] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old females only during 10 min [percentage of time] Locomotion in zero maze for males and females [n beam breaks] Novel open field behavior, locomotion in the center from 45-60 min for males [n beam breaks] Learning and memory performance, difference in latency to reach the platform between days 1 and 4 of acquisition of task using Morris water maze [s] Fear conditioning response, activity after third tone-shock pairing for males and females [n beam breaks/30 sec test] Fear conditioning response, activity during first tone-shock pairing for males and females [units] Locomotion in dark compartment of a light-dark box for females [cm] Learning and memory performance, difference in swim path to reach the platform between days 1 and 4 of acquisition of task using Morris water maze [cm] Novel open field behavior, locomotion in the center from 0-15 min for females [cm] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during 10 min [n beam breaks] Open field behavior, locomotion from 15-20 min for males and females [cm] Novel open field behavior, locomotion in the center from 0-15 min for females [n beam breaks] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males only during 10 min [percentage of time] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during last 5 min [n beam breaks] Novel open field behavior, locomotion in the center from 15-30 min for males and females [n beam breaks] Novel open field behavior, locomotion in the center from 45-60 min for males [cm] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during 10 min [beam breaks/sec] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males only during first 5 min [percentage of time] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during 10 min [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during first 5 min [n beam breaks] Anxiety assay, baseline untreated control (BASE group), latency to enter an open quadrant using an elevated zero maze in 60 to 120-day-old males and females [sec] Open field behavior, locomotion for entire test period for females [cm] Anxiety assay, transitions between light and dark sides of a light-dark box for females [n] Open field behavior, locomotion from 15-20 min for males [cm] Anxiety assay, untreated baseline, percentage of entries into closed arms of a plus maze for males and females [%] Novel open field behavior, locomotion in the center from 15-30 min for males and females [cm] Fear conditioning response, suppression of activity in altered context for males and females [units] Novel open field behavior, locomotion in the center from 0-60 min for males and females [n beam breaks] Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old females only during first 5 min [percentage of time] Open field behavior locomotion from 5-10 min for females [cm] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during first 5 min [n beam breaks] Novel open field behavior, locomotion in the center from 45-60 min for males and females [n beam breaks]

11433 11397 12014 11643 11512 11408 12337 11407 12015 11418 11644 11922 10915 11914 12350 10911 12338 11947 11509 10816 11916 11911 11641 10815 11767 12361 11923 11763 12348 12360 12021 11513 12364 12346 12351 12359 12355 11675 11648 11409 11970 12025 11910 11867 12336 11667 12339 12023

Anxiety assay, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to 120-day-old males only during last 5 min [percentage of time] Fear conditioning response, activity during third tone-shock pairing for females [units] Novel open field behavior, locomotion in the center from 0-60 min for males and females [cm] Fear conditioning response, suppression of activity in altered context for males [units] Anxiety assay, time in light side of a light-dark box for females [sec] Anxiety assay, time in dark side of a light-dark box for females [sec] Novel open field behavior, vertical activity (rears) from 15-30 min in the center for males [n beam breaks] Novel open field behavior, vertical activity (rears) from 0-60 min in the center for males [n beam breaks] Novel open field behavior, locomotion in the center from 30-45 min for males and females [n beam breaks] Fear conditioning response, baseline activity in apparatus for females [units] Novel open field behavior, locomotion in the center from 0-60 min for females [n beam breaks] Open field behavior, percentage of distance in the perimeter for males and females [%] Open field behavior, percentage of distance in the center for males and females [%] Anxiety assay, percentage of time in the light side of a light-dark box for females [%] Novel open field behavior, locomotion in the periphery from 0-60 min for males [n beam breaks] Fear conditioning response, activity during second tone-shock pairing for females [units] Novel open field behavior, vertical activity (rears) from 0-15 min in the center for males [n beam breaks] Novel open field behavior, locomotion in the center from 45-60 min for males and females [cm] Fear conditioning response, activity in altered context during presentation of cue for males [n beam breaks] Novel open field behavior, vertical activity (rears) in the periphery from 45-60 min for males [n beam breaks] Novel open field behavior, locomotion in the periphery from 0-60 min for males [cm] Novel open field behavior, locomotion in the center from 0-60 min for males [cm] Novel open field behavior, percentage of locomotion in the periphery for males [%] Novel open field behavior, vertical activity (rears) from 45-60 min in the center for males [n beam breaks] Fear conditioning, freezing response to conditioned cue after 24 hours (%) Open field behavior, percentage of distance in the perimeter for males [%] Open field behavior, percentage of distance in the center for males [%] Novel open field behavior, locomotion in the center from 0-60 min for males [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during 10 min [n beam breaks] Novel open field behavior, locomotion in the center from 30-45 min for males and females [cm] Fear response, response to contextual fear [% freezing] Novel open field behavior, locomotion in the center from 0-60 min for females [cm] Novel open fled behavior, habituation measured as the difference in locomotion between 0-5 min and 26-30 min periods for males and females [cm] Life span, longevity [days] (RWW winsorized outliers BXD2, BXD14, and DBA2J from 400, 500, and 175 days, respectively) Novel open field behavior, vertical activity (rears) from 30-45 min in the center for males [n beam breaks] Fear conditioning response, activity after second tone-shock pairing for females [n beam breaks/30 sec test] Anxiety assay, arm entries (total) in an elevated plus maze [n] Novel open field behavior, vertical activity (rears) in the periphery from 30-45 min for males [n beam breaks] Anxiety assay, untreated baseline, percentage of entries into closed arms of a plus maze for females [%] Anxiety assay, time in middle of an elevated plus maze for males [sec] Anxiety assay, untreated baseline, percent entries into open arms of an elevated plus maze for males [%] Motor coordination, time on dowel over a 2 min baseline test period for females [sec] Fear conditioning response, activity in altered context during presentation of cue for males and females [units] Pain response, thermal nociception, latency to paw withdrawal and licking response using a hot plate test [sec] Anxiety assay, baseline untreated control (BASE group), latency to enter an open quadrant using an elevated zero maze in 60 to 120-day-old females only [sec] Fear response, preconditioned stimulus [% freezing] Novel open field behavior, vertical activity (rears) in the periphery from 0-60 min for males [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males only during first 5 min [n beam breaks] Open field behavior, percentage of time in center for males and females [%] Open field behavior, percentage of time in perimeter for males and females [%] Open field behavior, vertical activity (rears) from 0-5 min for females [n beam breaks] Anxiety assay, entries into open quadrants of a zero maze for males and females [n] Open field behavior, vertical activity (rears) 10-15 min for males [n beam breaks] Anxiety assay, entries in closed quadrants of a zero maze for males and females [n]

12347 11656 11868 11396 11645 11642 11515 11502 12022 11650 11610 11933 11918 11647 11531 11655 11514 12027 11395 11529 11532 11354 11530 11517 11010 11419 11404 11353 12341 12026 10446 11611 10285 10112 11516 11658 10909 11528 11713 11467 11457 11562 11909 10897 12335 10447 11533 12349 11919 11934 11671 11949 11415 11948

Novel open field behavior, locomotion in the center from 0-60 min for females [n beam breaks] Anxiety assay, untreated baseline, entries into open arms of a plus maze for males [n] Anxiety assay, untreated baseline, percent time in closed arms of a plus maze for females [%] Anxiety assay, time in closed arms of an elevated plus maze for females [sec] Fear conditioning response, activity in altered context for males and females [units] Open field behavior, locomotion from 10-15 min for females [cm] Anxiety assay, untreated baseline, entries into closed arms of a plus maze for females [n] Fear conditioning response, activity in altered context for males [units] Novel open field behavior, percentage of locomotion in the periphery for males and females [%] Anxiety assay, percentage of time in light compartment of a light-dark test [%] Anxiety assay, percentage of locomotion in light side of a light-dark box for females [%] Startle response to loud acoustic stimulus Novel open field behavior, locomotion in the center from 0-60 min for females [cm] Open field behavior, vertical activity (rears) from 5-10 min for males and females [n beam breaks] Novel open field behavior, vertical activity (rears) in the periphery from 15-30 min for males [n beam breaks] Novel open field behavior, vertical activity (rears) from 45-60 min in the center for males and females [n beam breaks] Anxiety assay, open field behavior, duration in center of field [sec] Pain response, thermal nociception, 54 degree C hot plate latency baseline and post swim in maximum possible effect for males [%MPE=(postswim-baseline)/(cut-off-baseline)*100)] Anxiety assay, closed arm entries in an elevated plus maze test [n] Anxiety assay, time in the center of an elevated plus maze [sec] Open field behavior, locomotion from 15-20 min for females [cm] Fear conditioning response, activity after first tone-shock pairing for females [n beam breaks/30 sec test] Fear conditioning response, cue conditioning, activity suppression after third tone-shock pairing for females [units] Anxiety assay, open arm entries in an elevated plus maze test [n] Fear conditioning response, cue conditioning, activity suppression after third tone-shock pairing for males [units] Fear conditioning response, suppression of activity in altered context for females [units] Mean distance travelled (day 1 total distance-day 3 total distance) [cm] Open field behavior, percentage distance in the perimeter for females [%] Open field behavior, percentage of distance in the center for females [%] Open field behavior, vertical activity (rears) from 5-10 min for males [n beam breaks] Novel open fled behavior, habituation measured as the difference in locomotion between 0-5 min and 26-30 min periods for females [cm] Fear conditioning, freezing during conditioned stimulus exposure, 24 hr after conditioning [%] Open field behavior, percentage of time in perimeter for males [%] Open field behavior, percentage of time in center for males [%] Anxiety assay, time in open arms of a plus maze for males [sec] Anxiety assay, untreated baseline, percentage of time in open arms of a plus maze for males [%] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during last 5 min [beam breaks/sec] Fear conditioning response, contextual activity for females [units] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during last 5 min [n beam breaks] Pain response, thermal nociception, 54 degree C hot plate latency baseline and post swim in maximum possible effect for males and females [%MPE=(postswim-baseline)/(cut-off-baseline)*100)] Anxiety assay, entries in open quadrants of a zero maze for males [n] Anxiety assay, untreated baseline, percentage of time in open arms of a plus maze for females [%] Anxiety assay, entries in closed quadrants of a zero maze for males [n] Novel open field behavior, locomotion in the center from 45-60 min for females [n beam breaks] Novel open field behavior, vertical activity (rears) from 0-60 min in the center for males and females [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old males and females during first 5 min [beam breaks/sec] Anxiety assay, percentage of time in open arms of an elevated plus maze [%] Open field behavior, vertical activity (rears) from 5-10 min for females [n beam breaks] Activity level, locomotion in zero maze for females [n beam breaks] Fear conditioning response, activity after third tone-shock pairing for males [n beam breaks/30 sec test] Anxiety assay, untreated baseline, percent entries into open arms of an elevated plus maze for females [%] Novel open field behavior, locomotion in the periphery from 0-60 min for males and females [n beam breaks]

11757 11455 11715 11723 11906 11665 11711 11392 12044 10907 11646 11007 11758 11931 11527 12031 11015 10426 10896 10905 11666 11657 11660 10898 11403 11653 10038 11676 11661 11417 10332 10902 11420 11405 11468 11459 12343 11651 12340 10424 11435 11716 11434 11766 12016 12362 10908 11674 11690 11402 11714 12045

Anxiety assay, time in middle of an elevated plus maze for females [sec] Locomotor performance, duration on an accelerating rotarod, mean of three trials [s] Novel open field behavior, locomotion in the periphery from 0-60 min for males and females [cm] Open field behavior, vertical activity (rears) from 10-15 min for males and females [n beam breaks] Novel open field behavior, locomotion in the center from 45-60 min for females [cm] Anxiety assay, baseline untreated control (BASE group), latency to enter an open quadrant using an elevated zero maze in 60 to 120-day-old males only [sec] Motor coordination, rotarod performance, untreated training trial for males [sec] Novel open field behavior, vertical activity (rears) from 30-45 min in the center for males and females [n beam breaks] Open field behavior, percentage of time in center for females [%] Open field behavior, percentage of time in perimeter for females [%] Pain response, thermal nociception, 54 degree C hot plate latency baseline and post swim in maximum possible effect for females [%MPE=(postswim-baseline)/(cut-off-baseline)*100)] Open field behavior, time in corners for females [min] Novel open field behavior, vertical activity (rears) from 15-30 min in the center for males and females [n beam breaks] Fear conditioning, freezing response to conditioned cue after 48 hours (%) Pain response, 54 degree C hot plate latencies post-swim in male [sec] Anxiety assay, percentage of time in light side of a light-dark box for males [%] Anxiety assay, time in closed arms of an elevated plus maze for males and females [sec] Anxiety assay, untreated baseline, percent time in closed arms of a plus maze for males and females [%] Pain response, thermal nociception, 54 degree C hot plate latencies post-swim, male and female average [sec] Anxiety assay, untreated baseline, entries into closed arms of a plus maze for males and females [n] Novel open field behavior, vertical activity (rears) from 0-15 min in the center for males and females [n beam breaks] Floor preference baseline, intrinsic preference for the grid-textured floor for the control group of males [s/min] Novel open fled behavior, habituation measured as the difference in locomotion between 0-5 min and 26-30 min periods for males [cm] Anxiety assay, time in dark side of a light-dark box for males [sec] Anxiety assay, time in light side of a light-dark box for males [sec] Novel open field behavior, locomotion in the center from 30-45 min for females [n beam breaks] Pain response, 54 degree C hot plate latencies post-swim in female [seconds] Open field behavior, vertical activity (rears) from 0-20 min for males and females [n beam breaks] Floor preference baseline, intrinsic preference for the grid-textured floor for the control group of males [s/min] Open field behavior, vertical activity (rears) from 0-20 min for males [n beam breaks] Open field behavior, percentage time in perimeter minus time in corners for males [%] Novel open field behavior, locomotion in the center from 15-30 min for females [n beam breaks] Novel open field behavior, locomotion in the center from 30-45 min for females [cm] Open field behavior, vertical activity (rears) from 0-20 min for females [n beam breaks] Open field behavior, percentage of time in perimeter minus time in corners for males and females [%] Anxiety assay, entries into open quadrants of a zero maze for females [n] Anxiety assay, entries in closed quadrants of a zero maze for females [n] Novel open field behavior, vertical activity (rears) in the periphery from 0-15 min for males [n beam breaks] Anxiety assay, time in open arm of elevated plus maze [sec] Fear conditioning response, activity in altered context during presentation of cue for females [units] Open field behavior, vertical activity (rears) from 0-5 min for males and females [n beam breaks] Motor coordination, rotarod performance, untreated training trial for females [sec] Novel open field behavior, vertical activity (rears) in the periphery from 45-60 min for males and females [n beam breaks] Floor preference baseline, time on grid textured floor for experimental group of males [sec/min] Novel open field behavior, time in the field center [sec] Motor coordination, time on dowel over a 2 min baseline test period for males and females [sec] Novel open field behavior, vertical activity (rears) in the periphery from 30-45 min for males and females [n beam breaks] Open field behavior, time in corners for males and females [min] Hippocampus granule cell number [n total per side] Novel open field behavior, locomotion in the periphery from 0-60 min for females [n beam breaks] Novel open field behavior, vertical activity (rears) in the periphery from 0-15 min for females [n beam breaks] Learning and memory, spatial navigation, latency to reach platform using Morris water maze (test 1) [log

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sec] Anxiety assay, percentage of locomotion in light side of a light-dark box for males [%] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during 10 min [beam breaks/sec] Novel open field behavior, locomotion in the center from 15-30 min for females [cm] Novel open field behavior, percentage of locomotion in the periphery for females [%] Novel open field behavior, vertical activity (rears) in the periphery from 0-60 min for males and females [n beam breaks] Novel open field behavior, urinations for females [n/test period] Pain response, 54 degree C hot plate latencies baseline (before 3 min forced swim in 15 degree C water), male [seconds] Novel open field behavior, locomotion in the periphery from 0-60 min for females [cm] Novel open field behavior, vertical activity (rears) in the periphery from 15-30 min for females [n beam breaks] Fear conditioning, freezing in response to context exposure 48 hr after conditioning [%] Novel open field behavior, urinations for males and females [n/test period] Novel open field behavior, vertical activity (rears) from 45-60 min for males [n beam breaks] Novel open field behavior, vertical activity (rears) from 45-60 min for males and females [n beam breaks] Central nervous system, behavior: Learning and memory function, probe trial water maze time spent in swim path, day 5 [%] Anxiety assay, untreated baseline, entries into open arms of a plus maze for females [n] Anxiety assay, time in open arms of an elevated plus maze for females [sec] Pain response, thermal nociception, 54 degree C hot plate latency baseline test (before 3 min forced swim in 15 degree C water) for females [sec] Novel open field behavior, vertical activity (rears) in the periphery from 15-30 min for males and females [n beam breaks] Novel open field behavior, vertical activity (rears) in the periphery from 45-60 min for females [n beam breaks] Novel open field behavior, vertical activity (rears) from15-30 min in the center for females [n beam breaks] Prepulse inhibition of the acoustic startle response [%] Anxiety assay, locomotion in the light compartment relative to total in a light-dark test [%] Anxiety assay, time in closed quadrants of a zero maze for males and females [sec] Anxiety assay, time in open quadrants of a zero maze for males and females [sec] Novel open field behavior, vertical activity (rears) from 45-60 min in the center for females [n beam breaks] Open field behavior, vertical activity (rears) from 10-15 min for females [n beam breaks] Anxiety assay, time in middle of an elevated plus maze for males and females [sec] Novel open field behavior, vertical activity (rears) in the periphery from 0-60 min for females [n beam breaks] Anxiety assay, time in open arms of an elevated plus maze for males and females [sec] Anxiety assay, time in open quadrants of a zero maze for males [sec] Anxiety assay, time in closed quadrants of a zero maze for males [sec] Anxiety assay, untreated baseline, entries into open arms of a plus maze for males and females [n] Anxiety assay, percentage time in open quadrants of a zero maze for males and females [%] Novel open field behavior, vertical activity (rears) from 45-60 min for females [n beam breaks] Morris water maze behavior, mean swim speed during acquisition of task [cm/s] Response to auditory stimulus US in contextualized fear conditioning paradigm [% freezing] Novel open field behavior, vertical activity (rears) from 0-15 for females [n beam breaks] Learning and memory performance, time spent in target quadrant on day 5 (probe trial) using the Morris water maze [%] Novel open field behavior, vertical activity (rears) in the center from 0-60 min for males [n beam breaks] Fear conditioning response, cue conditioning, activity suppression after third tone-shock pairing for males and females [units] Novel open field behavior, vertical activity (rears) in the center from 0-60 min for males and females [n beam breaks] Anxiety assay, percentage time in light side of a light-dark box for males and females [%] Novel open field behavior, vertical activity (rears) from 30-45 min for males [n beam breaks] Anxiety assay, percentage of time in open quadrants of a zero maze for males [%] Novel open field behavior, vertical activity (rears) from min 30-45 for males and females [n beam breaks] Fear conditioning response, activity during first tone-shock pairing for females [units] Anxiety assay, untreated baseline, percent entries into open arms of an elevated plus maze for males and females [%] Open field behavior, vertical activity (rears) from 15-20 min for males [n beam breaks] Fear conditioning, freezing response to context after 48 hours (%) Novel open field behavior, vertical activity (rears) from 15-30 min for males [n beam breaks]

11389 12344 11768 11787 12047 11620 10420 11789 11784 10901 11877 11352 11866 10345 11712 11725 10419 12041 11786 11772 11008 10904 11952 11953 11774 11672 11981 11790 11982 11439 11438 11969 11951 11609 10814 10445 11606 10344 11355 11917 11869 11904 11351 11437 11865 11654 11971 11416 11011 11350

Learning and memory, spatial navigation, latency to reach platform using Morris water maze (test 3) [log sec] Novel open field behavior,vertical activity (rears) from 0-15 min for males and females [n beam breaks] Novel open field behavior, vertical activity (rears) in the center from 0-60 min for females [n beam breaks] Novel open field behavior, urinations for males [n/test period] Anxiety assay, time in light side of a light-dark box for males and females [sec] Anxiety assay, time in dark side of a light-dark box for males and females [sec] Motor performance, improvement in rotarod training measured as change in time on rod [sec] Open field behavior, time in corners for males [min] Motor coordination, rotarod performance, untreated training trial for males and females [sec] Fear conditioning response, activity in altered context for females [units] Anxiety assay, untreated baseline, percentage of time in open arms of plus maze for males and females [%] Anxiety assay, time in open quadrants of a zero maze for females [sec] Anxiety assay, time in closed quadrants of a zero maze for females [sec] Novel open field behavior, vertical activity (rears) from 0-15 min for males [n beam breaks] Novel open field behavior, vertical activity (rears) from 15-30 min for males and females [n beam breaks] Novel open field behavior, vertical activity (rears) in the periphery from 30-45 min for females [n beam breaks] Anxiety assay, number of closed arm entries using elevated plus maze [n] Open field behavior, locomotion [cm] % swim path spent in target quadrant Novel open field behavior, vertical activity (rears) in the periphery from 0-15 min for males and females [n beam breaks] Fear conditioning, freezing during consolidation period after final pairing of conditioned stimulus (CS) and unconditioned stimulus (US) [%] Anxiety assay, percentage of locomotion in light side of a light-dark box for males and females [%] Novel open field behavior, vertical activity (rears) from 30-45 min for females [n beam breaks] Open field behavior, vertical activity (rears) from 0-5 min for males [n beam breaks] Anxiety assay, percentage time in open quadrants of a zero maze for females [%] Novel open field behavior, vertical activity (rears) from 0-15 min in the center for females [n beam breaks] Mean latency to reach the platform [s] during acquisition of watermaze task Anxiety assay, transitions between light and dark compartments in light/dark test [n] Rotarod performance, baseline control (supplementary data to Brigman et al.) [sec] Open field behavior, vertical activity (rears) from 15-20 min for males and females [n beam breaks] Depression assay, immobility from 2-10 min in a 10 min Porsolt forced swim test [%] Motor coordination, time on dowel over a 2 min baseline test period for males [sec] Sleep need, slow wave sleep delta EEG power after 6 hrs sleep deprivation [%] Open field behavior, percentage time in perimeter minus time in corners for females [%] Learning and memory, spatial navigation, latency to reach platform using Morris water maze (test 2) [log sec] Novel open field behavior, vertical activity (rears) from 15-30 min for females [n beam breaks] Open field behavior, vertical activity (rears) from 15-20 min for females [n beam breaks] Anxiety assay, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60 to 120-day-old females only during first 5 min [beam breaks/sec] Novel open field behavior, vertical activity (rears) from 0-60 min in the center for females [n beam breaks] Anxiety assay, untreated baseline, entries into closed arms of a plus maze for males [n] Pain response, thermal nociception, 54 degree C hot plate latency baseline test (before 3 min forced swim in 15 degree C water) for males and females [sec] Novel open field behavior, vertical activity (rears) from 30-45 min in the center for females [n beam breaks] Learning and memory performance, mean path length to reach the platform during acquisition of task using Morris water maze [cm]

10415 11863 11612 11363 11902 11899 11005 11406 11979 11649 11973 11696 11695 11349 11864 11785 11013 11014 10807 12040 10900 11903 11608 11414 11694 11771 10808 10917 11004 11930 10903 11305 10143 11669 10414 11607 11673 12342 11759 11454 10418 11773 10810