Jul 10, 2017 - ... Faculty of Veterinary Medicine, Cumhuriyet University, TR-58140 Sivas - TURKEY .... cattle in 25 different farms located in Elazig province,.
Article in Press
Kafkas Univ Vet Fak Derg x (x): xxx-xxx, 2017 DOI: 10.9775/kvfd.2017.17995
Kafkas Universitesi Veteriner Fakultesi Dergisi Journal Home-Page: http://vetdergikafkas.org Online Submission: http://submit.vetdergikafkas.org
Research Article
Development of A Multiplex PCR Method for Direct Detection of Common Mastitis Pathogens in Bovine Milk Samples [1] [2] Recep KALIN 1 Murat KARAHAN 1,2 Mehmet Nuri ACIK 3 Bülent TASDEMIR 4 Burhan CETINKAYA 5 This work was supported by General Directorate of Agricultural Research and Policy, Project number: TAGEM/HS/01/02/08/129 This work has been presented as poster in 10th National Veterinary Microbiology Congress, 24-27 September 2012, Kusadasi, Aydin, Turkey 1 Department of Microbiology, Faculty of Veterinary Medicine, Cumhuriyet University, TR-58140 Sivas - TURKEY 2 Faculty of Veterinary Medicine, Kyrgyz-Turkish Manas University, KG-720044, Bishkek-KYRGYZSTAN 3 Department of Microbiology, Faculty of Veterinary Medicine, Bingol University, TR-12000 Bingol - TURKEY 4 Veterinary Control Institute, TR-23200, Elazig - TURKEY 5 Department of Microbiology, Faculty of Veterinary Medicine, Firat University, TR-2311, Elazig - TURKEY [1] [2]
Article Code: KVFD-2017-17995 Received: 05.05.2017 Accepted: 10.07.2017 Published Online: 10.07.2017 Citation of This Article Kalin R, Karahan M, Acik MN, Tasdemir B, Cetinkaya B: Development of a multiplex PCR method for direct detection of common mastitis pathogens in bovine milk samples. Kafkas Univ Vet Fak Derg, 2017 (in Press). DOI: 10.9775/kvfd.2017.17995
Abstract The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli and Mycoplasma bovis) from milk samples. Of the 200 California Mastitis Test (CMT) positive milk samples, 45 (22.5%), 21 (10.5%) and 11 (5.5%) were detected as positive for the presence of S. aureus, S. agalactiae and E. coli by culture, respectively. In mPCR by DNA isolation method optimised here, S. aureus, S. agalactiae and E. coli were detected in 26.5% (53/200), 12% (24/200) and 6% (12/200) of the milk samples, respectively. The abovementioned agents were observed in similar proportions when the samples were analysed by a commercial DNA isolation kit. On the other hand, M. bovis was not detected in any of the milk samples by either culture or mPCR methods. A significant difference was determined between the results of culture and mPCRs (P
