Development of resazurin microtiter assay for drug ... - Springer Link

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Feb 28, 2006 - cytotoxicity of the reference drugs nifurtimox and benznidazole was measured with this assay and compared to the microscopic counting ...

Parasitol Res (2006) 99: 103–107 DOI 10.1007/s00436-006-0126-y

ORIGINA L PA PER

Miriam Rolón . Celeste Vega . José A. Escario . Alicia Gómez-Barrio

Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes Received: 14 October 2005 / Accepted: 7 December 2005 / Published online: 28 February 2006 # Springer-Verlag 2006

Abstract A quantitative colorimetric assay using the oxidation–reduction indicator resazurin was developed to measure cytotoxicity of compounds against the protozoan parasite Trypanosoma cruzi. This method is based on the detection of colorimetric changes caused by the oxidation (blue) and reduction (pink) capabilities of resazurin dye, an indicator for metabolic cell function. To validate the assay, the experimental conditions were adjusted, such as number of parasites, dye concentration, and time of incubation, with respect to linearity and lower limit of detection. We found that absorbances increased linearly, with the plating density of parasites as low as 5–100×104/well (r=0.99; p

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