with the development of T cell lymphoma, nasopharyngeal cancer (NPC), and EBV-associated gastric cancer (EBVaGC). This study assessed the expression of ...
MOLECULAR MEDICINE REPORTS 13: 4151-4158, 2016
Differential expression of EBV proteins LMP1 and BHFR1 in EBV‑associated gastric and nasopharyngeal cancer tissues AILIANG WANG1,2, WEI ZHANG2, MENG JIN2, JIANBO ZHANG3, SHENG LI3, FENG TONG3 and YANBING ZHOU1 1
Department of General Surgery, The Affiliated Hospital of Medical College of Qingdao University, Qingdao, Shandong 266003; 2Department of General Surgery, The Affiliated Hospital of Jining Medical University, Jining, Shandong 272000; 3Department of General Surgery, Shandong Tumor Hospital, Jinan, Shandong 250117, P.R. China Received February 5, 2015; Accepted January 29, 2016 DOI: 10.3892/mmr.2016.5087 Abstract. Epstein‑Barr virus (EBV) infection is associated with the development of T cell lymphoma, nasopharyngeal cancer (NPC), and EBV‑associated gastric cancer (EBVaGC). This study assessed the expression of the EBV‑associated proteins latent membrane protein 1 (LMP1) and BamHI‑A rightward frame 1 (BARF1) in NPC and EBVaGC tissue specimens and determined their association with clinicopathological data, microvessel density (MVD) and micro‑lymphatic vessel density (MLVD). This study collected 600 gastric cancer and 75 NPC tissue samples. EBV infection was assessed using in situ hybridization, and LMP1 and BARF1 expression was assessed using immunohistochemistry. The levels of MVD and MLVD were assessed using immunostaining of vascular endothelial growth factor (VEGF)‑C, CD34, and lymphatic vessel endothelial receptor 1 (LYVE‑1). Among the 600 gastric cancer cases, 30 were positive for EBV infection, which was shown to be associated with the age of patients (P=0.073), tumor differentiation (P60 345 13 334 Tumor differentiation ﹤0.0001 Well 68 8 60 15.69 Moderate 124 12 112 Poor 408 10 398 Tumor location ﹤0.0001 Gastric cardia region 102 12 90 14.99 Gastric body region 139 10 129 Gastric antral region 359 8 351 Lymph node metastasis 0.0001 Yes 580 21 559 40.39 No 20 9 11 EBV, Epstein‑Barr virus.
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B
Figure 1. In situ hybridization detection of EBV‑encoded small RNA 1 to indicate Epstein‑Barr virus infection in gastric cancer and nasopharingeal carcinoma tissues. (A) Positive and (B) negative expression.
vessels were labeled by anti‑LYVE‑1 antibody and reviewed and counted as same as MVD. Statistical analysis. All statistical analyses were performed using SPSS software, version 18.0 (SPSS Inc., Chicago, IL, USA). All data were analyzed using the Student's t‑test. P
