Diminution of the gut resistome after a gut microbiota-targeted dietary intervention in obese children Guojun Wu, Chenhong Zhang, Jing Wang, Feng Zhang, Ruirui Wang, Jian Shen, Linghua Wang, Xiaoyan Pang, Xiaojun Zhang, Liping Zhao, Menghui Zhang *
Affiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R.China
* Corresponding authors: Menghui Zhang e-mail:
[email protected]
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Supplementary Figure S1 | The altered abundances of ARGs acting via three different mechanisms. The ARGs were assigned to the three major resistance mechanisms according to ARDB. The abundances of ARGs conferring resistance for the same mechanism were summed. The boxes denote the interquartile range (IQR) between the first and third quartiles (25th and 75th percentiles, respectively), and the line inside the boxes denotes the median. The whiskers denote the lowest and highest values within 1.5 times of the IQR from the first and third quartiles, respectively. The samples are displayed as the dot on the left. Statistical analysis was performed with a two-tailed Wilcoxon matched-pairs signed rank test (n = 35). The P-values were adjusted (Benjamini & Hochberg 1995).
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Supplementary Figure S2 | The altered profiles of ARG carriers after dietary intervention. PCA score plot based on the profile of 120 ARG carriers showing a trend for segregation between samples before and after dietary intervention (log-transformed, PERMONOVA P = 0.0584, permutation = 9999).
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Supplementary Table S1 |The information of the 96 ARGs with significant changes.
