Histone Acetylation Level and Histone ... - KoreaMed Synapse

0 downloads 0 Views 2MB Size Report
Feb 10, 2014 - Purpose: The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase ...
Original Article

http://dx.doi.org/10.3349/ymj.2014.55.5.1333 pISSN: 0513-5796, eISSN: 1976-2437

Yonsei Med J 55(5):1333-1340, 2014

Histone Acetylation Level and Histone Acetyltransferase/ Deacetylase Activity in Ejaculated Sperm from Normozoospermic Men Jee Hyun Kim,1,2 Byung Chul Jee,1,2 Jang Mi Lee,1 Chang Suk Suh,1,2 and Seok Hyun Kim2,3 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam; 2 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul; 3 Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.

1

Received: December 16, 2013 Revised: February 10, 2014 Accepted: March 3, 2014 Corresponding author: Dr. Byung Chul Jee, Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82 Gumi-ro 173beon-gil, Bundang-gu, Seongnam 463-707, Korea. Tel: 82-31-787-7254, Fax: 82-31-787-4054 E-mail: [email protected] ∙ The authors have no financial conflicts of interest.

Purpose: The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Materials and Methods: Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay. Results: The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p